Abstract: In a method for measuring luminescence of a biological sample utilizing two different luminescence reagents, the sample is agitated to improve mixing with the second luminescence reagent, allowing for a shorter delay time between injection of the second reagent and measurement of the resulting luminescence activity. The improved mixing may also allow for a shorter measurement time, thereby improving throughput when assaying a large number of samples.

Abstract: A method to convert at least a majority of native cellulose I? to cellulose IIII in pretreated or untreated densified biomass is disclosed. The densified biomass (cellulosic or lignocellulosic) is pretreated with anhydrous liquid ammonia or liquid ammonia comprising a solution of at least 80% ammonia. The pretreating can be performed at a temperature from about 4° C. to about 140° C. and a pressure from about 14.7 to about 200 psi. In one embodiment, the densified cellulosic converting pretreatment process is followed by lignin extraction (LE). The total moisture content of the densified biomass and the solution is 30% or less and a weight ratio of liquid ammonia to the densified biomass is from about 0.3:1 to about 2:1. Various products and systems are also disclosed.

Abstract: Methods are provided to facilitate the detection and enumeration of neutrophils in bodily fluids, including milk. The methods incorporate the fluorescent staining of neutrophils using fluorogenic enzyme substrates, the imaging of fluorescent cells using a digital device, and the electronic counting of the cells.

Abstract: The present invention relates to a method of treating dermatitis, in particular radiation-induced dermatitis, with extracellular vesicles, in particular exosomes obtained from human cardiospheres or cardiosphere-derived cells. The present invention also provides a formulation comprising extracellular vesicles, in particular exosomes obtained from human cardiospheres or cardiosphere-derived cells, for use in the treatment of dermatitis, in particular radiation-induced dermatitis.

Abstract: The present invention pertains to a method for the mass cultivation of cells, and a cell cultivation device and kit. The present invention further pertains to a continuous cell cultivation method and a continuous cell cultivation device in which a carrier is used.

Abstract: The present invention relates to a microvesicle that is derived from nucleated mammalian cells, which are smaller than the nucleated cells. The microvesicles of the present invention can be used in the delivery of a therapeutic or diagnostic substance to specific tissues or cells, and more particularly, relates to microvesicles derived from monocytes, macrophages, dendritic cells, stem cells or the like, which can be used to deliver specific therapeutic or diagnostic substances for treating and/or diagnosing tissue associated with cancer, diseased blood vessels, inflammation, or the like.

Abstract: Provided is a dental pretreatment material for dental tissue regeneration by use of dental pulp stem cells, particularly a dental pretreatment material effectively enabling dental tissue regeneration even by use of dental pulp stem cells of middle-aged or older individuals. The dental pretreatment material is characterized by comprising a serine protease, specifically trypsin. The dental pretreatment material comprising trypsin is used as an injection into a root canal before a root canal filling material comprising dental pulp stem cells and an extracellular matrix is inserted into the root canal as an attempt to regenerate a dental pulp and a dentin. The root canal filling material includes an ALK5 inhibitor, a CCR3 antagonist, or a CCL11 neutralizing antibody.

Abstract: This invention is directed to the use of a protein hydrolysate, and in particular an egg lysozyme hydrolysate to assist an animal, including a human in overcoming an addition, or by breaking an unwanted habit.

Abstract: The purpose of the present invention is to provide a method for isolating, removing and analyzing cells. Provided is a method for isolating, removing and analyzing cells by filtering a liquid specimen containing cells through a porous polyimide film, and subjecting the cells captured by the film which did not pass through the porous polyimide film, or the cells in the liquid specimen which did pass through the porous polyimide film, to an examination of one or more cell properties selected from a group consisting of cell number or type, internal or external cell structure, type or amount of cell surface antigen, type or amount of material secreted from cells, cell adhesion, and cell survival rate.

Abstract: The present invention relates to the use of insulin-producing cells encapsulated in silanized hydroxypropyl methylcellulose (Si-HPMC) for the treatment of type 1 diabetes. Methods and kits are also provided for restoring and/or maintaining euglycemia in type 1 diabetic patients and in type 1 prediabetic patients.

Abstract: The present invention is directed to a method and kit for inactivation of acid-fast bacteria. In some embodiments, the method may include: transferring a sample from a liquid culture containing acid-fast bacteria to a first tube, wherein the first tube comprises a body, a first end to the body having an opening, and a second end to the body having a frustoconical portion ending in a concave tip; centrifuging the tube to pellet the acid-fast bacteria in the concave tip and subsequently decanting a supernatant; resuspending the acid-fast bacteria pellet in alcohol; transferring the suspension to a second tube containing beads; agitating the second tube to disrupt acid-fast bacteria cells; and incubating the suspension to inactivate the acid-fast bacteria in the test sample. The method may also include identifying the acid-fast bacteria with mass spectrometry.

Abstract: The invention relates to a long term cell culturing method and a cell culturing apparatus and kit that employ a porous polyimide film. The invention further relates to a cell cryopreservation method and kit employing the porous polyimide film.

Abstract: An agent for removing malodor from a painting booth includes a volatile organic compound (VOC) degrading microorganism and a volatile fatty acid (VFA) degrading microorganism. The VOC degrading microorganism is configured to degrade VOC. The VFA degrading microorganism is configured to degrade VFA generated when the VOC is degraded. A method of removing malodor is also disclosed.

Abstract: A method of delivering exosomes and other micro vesicles to a biological target includes the steps of 1) providing blood, (2) separating plasma from the provided blood, and (3) separating the solution with the exosomes therefrom, (4) encapsulating the exosomes, and (5) delivering the exosomes. The step of encapsulation may be accomplished by water bead process, alginate bead process, spray drying bead formation, or plating. The biological target may be a human or animal heart, bone/joint, wound or skin. A method of encapsulating an exosome, a method of using an encapsulated exosome, compositions including encapsulated exosomes, and an encapsulated exosome per se are also disclosed.

Abstract: The present disclosure relates to a novel musculoskeletal stem cell (MSSC) differentiated from an ESC (embryonic stem cell) or an iPSC (induced pluripotent stem cell). The musculoskeletal stem cell of the present disclosure can be easily induced from a human embryonic stem cell or a human-derived pluripotent stem cell and can be effectively differentiated not only into bone but also into cartilage, tendon and muscle. Accordingly, it can be usefully used for prevention or treatment of various musculoskeletal diseases.

Abstract: The present invention relates to methods for generating mature insulin-producing ?-like cells comprising the steps: (a) isolating, purifying and/or enriching ?-cell progenitor cells from a population of cells; (b) differentiation of the ?-cell progenitor cells into immature insulin-producing ?-like cells; and (c) maturation of the immature insulin-producing ?-like cells into mature insulin-producing ?-like cells, comprising exposing the immature insulin-producing ?-like cells to calcitriol or an analogue thereof. More particularly, the present invention relates to methods for generating mature insulin-producing ?-like cells for use in treating diabetes.

Abstract: A Hunter syndrome therapeutic agent contains a first composition to be intravenously injected and a second composition to be subcutaneously injected. The agent can reduce the number of visits to the hospital by patients with Hunter syndrome to twice a month or less. It maintains a medicinal effect equivalent to or greater than that of a conventional once-a-week IV injection, increases drug-taking compliance of patients in comparison to conventional therapeutic agents and treatment methods, and enables enhanced patient welfare and convenience.

Abstract: The present disclosure relates generally to supplement compositions containing citrulline and taurine at a weight ratio of 4.56:1 to 2.57:1 which have been demonstrated to have a pleasant flavor and can enhance the exercise endurance in individuals in a synergistic manner.

Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.

Abstract: In a method for producing a cell concentrate using a cell suspension treatment system including a storage container of a cell suspension, which has a solution inlet port, a circulation outlet port, and a circulation inlet port, a cell suspension treatment device for concentrating the cell suspension by separating liquid from the cell suspension by filtration, the device including a container having a cell suspension introduction port, a cell suspension lead-out port, and a filtrate outlet, which is filled with a hollow fiber separation membrane, a circulation circuit for concentrating the cell suspension while circulating the cell suspension between the storage container and the cell suspension treatment device, a collection container of a cell concentrate obtained by concentration, a collection path for feeding the cell concentrate to the collection container, an injection path for injecting a solution into the solution inlet port of the storage container, and a detecting unit.