Abstract: Methods are provided for detecting enzymatic activity of various lysosomal storage enzymes using substrates that include: a sugar moiety; a linker moiety allowing the conjugation of sugar moiety with the remaining structure of the substrate; and two or more fatty acid chains or derivatives thereof at least one of which is sufficiently structured to provide improved solubility in aqueous or organic solvent systems. Also provided are internal standards, and inhibitors for use in detecting or reducing enzymatic activity using the inventive substrates.
Type:
Grant
Filed:
June 21, 2016
Date of Patent:
October 26, 2021
Assignees:
University of Washington through its Center for Commercialization, PerkinElmer Health Sciences, Inc.
Inventors:
Alexander Cherkassky, Jason Coumoyer, Michael Gelb
Abstract: A method for detecting at least one microorganism that may be present in a sample, comprising the steps of: a) bringing into contact, in a container: a culture medium that enables the growth and/or detection of microorganisms, said sample and a sensitized solid support; b) subjecting the whole to a temperature that promotes the growth and/or detection of microorganisms; and c) observing, in real time, the appearance of an agglutination indicating the presence of the microorganism(s) or confirming said presence when said microorganisms are detected in said culture medium, when step b) has been completed, and a method for detecting and identifying at least one target microorganism that may be present in a sample.
Type:
Grant
Filed:
March 12, 2009
Date of Patent:
August 17, 2021
Assignee:
BIOMERIEUX
Inventors:
Bruno Colin, David Mosticone, Jean-Claude Raymond, Thierry Sofia, Antoine Vimont
Abstract: This invention provides gels and matrices having a rigidity in the range of 150-750 Pa, methods of manufacturing same, and method of preserving a mesenchymal stem cell population or studying mesenchymal stem cells, comprising same.
Type:
Grant
Filed:
June 30, 2008
Date of Patent:
August 10, 2021
Inventors:
Makoto Funaki, Paul A. Janmey, Jessamine Winer
Abstract: A method for the anti-senescence of and/or rejuvenating a stem cell is provided. The method comprising treating the stem cell with trans-cinnamaldehyde (TC). A kit is also provided. The kit comprising (1) a first part, comprising a stem cell; (2) a second part, comprising TC; and (3) a culture medium of the stem cell, placed in at least one of the first part, the second part, and a third part. In addition, a method for stem cell therapy is provided. The method comprising administering to a subject in need an effective amount of a stem cell, wherein the stem cell has been treated with TC before being administered.
Type:
Grant
Filed:
May 11, 2017
Date of Patent:
June 1, 2021
Assignee:
GWO XI STEM CELL APPLIED TECHNOLOGY CO., LTD.
Abstract: The present invention relates to a method of processing allograft skin for transplantation and a cryopreserved allograft skin produced thereby. More specifically, the present invention relates to a method in which a cryoprotectant is prepared by adding sucrose to basic constituents comprising dimethyl sulfoxide, an animal cell culture medium and fetal bovine serum, and then the resulting solution is used to subject skin tissue for transplantation to a freezing process.
Abstract: The present invention relates to apparatus, methods, and applications for treating wastewater, and more particularly to biological processes for removing pollutants from wastewater. This invention further relates to apparatus and methods for growing microbes on-site at a wastewater treatment facility, and for economically inoculating sufficient microbes to solve various treatment problems rapidly.
Abstract: The present invention relates to a device and method for the detection of mastitis or other disease from a body fluid of a mammal for example from cow's milk. The device and method relates to a wedge microfluidic chamber for using a minimal amount of fluid and being able to use the device to observe leukocytes in a mono-layer for the purpose of disease detection, cell counts or the like.
Type:
Grant
Filed:
December 13, 2013
Date of Patent:
April 20, 2021
Assignee:
Advanced Animal Diagnostics, Inc.
Inventors:
Rodolfo R. Rodriguez, Charles F. Galanaugh
Abstract: The claimed subject matter comprises a device to collect and preserve cells comprising of: (1) a collection container comprised of a tube having an open end and a closed end, a closure in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and (2) compounds including an anticoagulant agent and a fixative agent, wherein the compounds are in a sufficient amount to preserve said cells' original morphology and antigenic sites without significant dilution of said cells, and thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment.
Abstract: A process providing a method to create 3D scaffolds using nano-scale fibers, comprising: deposition and alignment of a plurality of electrospun fiber layers on a substrate; application of a photosensitive biomedical polymer liquid to each fiber layer deposited on said substrate; deposition and cross-alignment of a plurality of electrospun fiber layers on said substrate; retaining said polymer liquid in place using said cross-aligned fiber layers; curing said polymer liquid on top of each fiber layer using UV light.
Abstract: Variations in the differentiation and lineage potential of stem cells, including mesenchymal stem cells, currently limit their therapeutic use. The ability to identify, isolate, and specifically amplify stem cell populations with desired differentiation potential would contribute the use of stem cells in research and therapy. The present invention discloses a method of assessing differentiation potential of stem cells by measuring the differential expression of antigens CD 146 and NG2 on the stem cells. The correlation between CD 146 and NG2 expression and differentiation and trilineage potential is explored. The invention also discloses methods to specifically amplify or enrich stem cells with desired differentiation potential, monitor the differentiation potential of a heterogeneous stem cell population, quantify the heterogeneity in differentiation potential of a stem cell culture, and remove stem cells with specific differentiation potentials from a heterogeneous cell culture.
Type:
Grant
Filed:
December 21, 2011
Date of Patent:
March 2, 2021
Assignee:
THE ADMINISTRATORS OF THE TULANE EDUCATIONAL FUND OFFICE OF TECHNOLOGY TRANSFER AND INTELLECTUAL PROPERTY DEVELOPMENT
Abstract: The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.
Type:
Grant
Filed:
June 14, 2019
Date of Patent:
December 1, 2020
Assignee:
THE FYNDER GROUP, INC.
Inventors:
Mark A. Kozubal, Richard E. Macur, William P. Inskeep
Abstract: The present invention relates to a process for preparing erythrocytes loaded with one or more substance of pharmaceutical interest. The present invention is also directed to loaded erythrocytes thus obtained and pharmaceutical compositions comprising said population of loaded erythrocytes as well as therapeutic application thereof, in particular in the treatment of Ataxia telangiectasia.
Type:
Grant
Filed:
May 9, 2014
Date of Patent:
December 1, 2020
Assignee:
ERYDEL S.P.A.
Inventors:
Giovanni Mambrini, Luca Benatti, Giovanni Capogrossi, Marco Mandolini
Abstract: Sample-collecting devices, articles and methods of use are disclosed. Premoistened sample-collecting devices comprise 1,2-propanediol as a humectant, which promotes the retention of a liquid solution on the sample-collecting device during storage. 1,2-propanediol as humectant can be compatible with proteins and other reagents used to detect an analyte.
Type:
Grant
Filed:
March 1, 2016
Date of Patent:
November 24, 2020
Assignee:
3M INNOVATIVE PROPERTIES COMPANY
Inventors:
Nerys Bennion, James Watkin, Russell M. I. Thomas, William J. Simpson
Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to TMJ implantation materials and implants (e.g., temporomandi bular joint (TMJ) methods of making TMJ implantation materials and implants, methods of forming a TMJ implantation material or an implant, and the like.
Type:
Grant
Filed:
December 7, 2012
Date of Patent:
November 10, 2020
Assignee:
University of Florida Research Foundation, Incorporated
Abstract: The present invention relates to a method for detecting the presence of expanded-spectrum ß-lactamase (ß-lactamase hydrolyzing expanded-spectrum cephalosporin)-producing bacteria in a sample, said method comprising the steps of: a) performing cell lysis on a test sample in order to obtain an enzymatic suspension; b) reacting a fraction of the enzymatic suspension obtained in step a) with a reagent kit, said reagent kit comprising —expanded-spectrum ß-lactamase substrate selected from the group consisting of cephalosporins, aztreonam, and cephamycins, —a pH color indicator which will change color when the pH of the reaction mixture is comprised between 6.4 and 8.4, wherein a color change after step b) indicates the presence of expanded-spectrum ß-lactamase-producing bacteria in the test sample. The invention also relates to a reagent kit, to a microtiter plate and to their uses in detecting the presence of expanded-spectrum ß-lactamase producers in a test sample.
Type:
Grant
Filed:
November 16, 2012
Date of Patent:
October 6, 2020
Assignees:
INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), UNIVERSITÉ PARIS-SACLAY, ASSISTANTE PUBLIQUE HOPITAUX DE PARIS (APHP)
Abstract: This invention relates to the use of Lactobacillus delbrueckii to elicit a plant cell of an Iridaceae and the use of these elicited cells in treating sensitive skins.
Abstract: A method to generate dioxolanes from renewable feedstocks, and more specifically, these oxygenated hydrocarbons can be used as gasoline-range fuels and diesel additives.
Type:
Grant
Filed:
August 24, 2015
Date of Patent:
July 14, 2020
Assignee:
The Government of the United States of America as represented by the Secretary of the Navy
Inventors:
Benjamin G. Harvey, Heather A. Meylemans
Abstract: A microfluidic system for causing perturbations in a cell membrane, the system including a microfluidic channel defining a lumen and being configured such that a cell suspended in a buffer can pass therethrough, wherein the microfluidic channel includes a cell-deforming constriction, wherein a diameter of the constriction is a function of the diameter of the cell.
Type:
Grant
Filed:
October 17, 2012
Date of Patent:
June 30, 2020
Assignee:
Massachusetts Institute of Technology
Inventors:
Armon R. Sharei, Andrea Adamo, Robert S. Langer, Klavs F. Jensen
Abstract: Probiotic compositions that comprise one or more bacteria species in spore form, a thickener to form a stabilized suspension and to preferably act as a prebiotic, one or more acids or salts of acids, and optionally a water activity reducer. A system for delivering probiotic compositions by gravity feed or non-contact pump to a point of consumption by a plant or animal, preferably in conjunction with acidified drinking water, comprising a collapsible container with attachable tubing that prevent contamination of the probiotic composition within the container. Delivery may be actuated in response to a timer, motion detector, fluid level sensor, RFID tag, or other mechanism to periodically or continuously dispense a dosage of probiotic composition directly to the soil surrounding a plant or to the water or feed for an animal. A method for increasing beneficial bacteria in an animal's GIT comprises adding probiotics to acidified drinking water.
Type:
Grant
Filed:
October 27, 2014
Date of Patent:
May 19, 2020
Assignee:
NCH Corporation
Inventors:
Scott Martell Boyette, Judith Gayle Pruitt, John Knope, Adrian Denvir, Charles Greenwald, Alex Erdman
Abstract: The present invention relates to edible webs comprising microorganisms, such as probiotics. In particular, the present invention relates to an edible web having microorganisms, such as bacteria, probiotic bacteria, bacteriophages or viruses printed thereon, e.g. by the use of inkjet printing. In addition the invention relates to methods of producing such edible webs, to various products comprising the edible webs as well as to use of the edible webs comprising microorganisms.