Abstract: Provided are substantially dephosphorylated forms of lysosomal storage disease (LSD) proteins, including dephosphorylated forms of iduronate-2-sulfatase (IDS, or I2D) and iduronidase (IDU), having increased ability to traverse or penetrate the blood brain barrier (BBB) relative to phosphorylated forms of the protein, and p97 conjugates thereof. Also provided are compositions comprising such dephosphorylated LSD proteins and p97 conjugates, and methods of use thereof, for instance, to treat any one or more lysosomal storage diseases, such as Hunter Syndrome (or MPS Type II).

Abstract: There is provided a dried L-glutamate oxidase composition containing an L-glutamate oxidase, which is stable even if it is stored for such a long term as one year or longer. The composition is a dried composition, preferably lyophilized product, containing an L-glutamate oxidase and a disaccharide. A preferred example of the disaccharide is lactose. Content of the disaccharide per 100 U of the L-glutamate oxidase is preferably 0.5 to 50 mg.

Abstract: The present invention provides a lactobacillus strain-containing pharmaceutical composition and food composition for improving of kidney disease and inhibiting inflammation, which comprises an isolated lactic acid bacteria strain. The isolated lactic acid bacteria strain is at least one selected from a group including BLI-02 strain, CGMCC No. 15212, Bifidobacterium longum subsp. infantis, TYCA06 strain, CGMCC No. 15210, Lactobacillus acidophilus, VDD088 strain, CGMCC No. 15211, Bifidobacterium bifidum, and combinations thereof.

Abstract: A system and method for intra-operative sample analysis including acquiring a tissue sample, preparing the tissue sample for mass spectrometry imaging, conducting a mass spectrometry imaging procedure on the tissue sample to produce an image, and analyzing the image to determine the presence or absence of a bio-marker.

Abstract: A nerve growth promoter and an antioxidant containing a degradation product obtained by degrading a composition containing a hyaluronic acid and a protein with a protease. A would treatment agent containing an ethyl acetate extract of a degradation product obtained by degrading a composition containing a hyaluronic acid and a protein with a protease.

Abstract: The present invention relates to a process for freeze drying a bacteria-containing concentrate. Further, the present invention relates to the freeze dried concentrates per se.

Abstract: The invention is applicable within the food and pharmaceutical industry. More specifically, it relates to a novel strain of the species Bifidobacterium animalis subsp. lactis CECT 8145, the cell components, metabolites and secreted molecules thereof, which, incorporated into food and/or pharmaceutical formulations, can be used in the treatment and/or prevention of excess weight and obesity and related diseases such as metabolic syndrome, hypertension, glycemia, inflammation, type 2 diabetes, cardiovascular diseases, hypercholesterolemia, hormonal alterations, infertility, etc.

Abstract: The methods of harvesting cells are provided, wherein the methods comprise introducing a processing material and a source material into a processing loop. The processing loop comprises a processing chamber and a filtering device. The processing material and the source material are circulating through the processing chamber and the filtering device, wherein the processing chamber has a mass; balancing an influx of the processing material into the processing chamber with a permeate flux of the filtering device to maintain the mass of the processing chamber at a constant value; and collecting the cells in a collection chamber. Cell harvesting devices are also provided for processing and harvesting cells using a control law to balance the mass of the processing chamber through the entire process.

Abstract: A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

Abstract: The present disclosure provides methods, and related kits, for directing cell attachment and spreading on a substrate and inducing isotropic spreading of cells; provides methods, and related kits, for cell sorting; and further provides methods, and related kits, for guided induction of stem cell differentiation.

Abstract: Provided is an agent for treating or preventing a corneal endothelial disorder wherein cell proliferation is required. More specifically, provided is an agent for treating or preventing a corneal endothelial disorder, wherein cell proliferation is required, said agent comprising a p38MAP kinase inhibitor. In a preferred embodiment, the corneal endothelial disorder is a wound. In a preferred embodiment, the p38MAP kinase inhibitor is soluble in water. The p38MAP kinase inhibitor may comprise 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole (SB203580) or a salt thereof.

Abstract: Aspects of the invention include WBC analysis reagents, systems and methods that can be used for analyzing a sample of whole blood to identify, classify, and/or quantify white blood cells (WBC) and WBC sub-populations in the sample. The WBC analysis reagents of the present disclosure generally include at least one membrane-permeable fluorescent dye, a WBC protecting reagent, and a surfactant. In some embodiments, the WBC reagents include a suitable amount of an osmolality adjusting component to adjust the osmolality of the WBC reagent into a desired range.

Abstract: A water soluble polymer, in particular polysialic acid (PSA) or a modified PSA (mPSA), is conjugated to an oxidized carbohydrate moiety of a glycoprotein other than a blood coagulation protein or to a ganglioside or drug delivery system by contacting the oxidized carbohydrate moiety with the water soluble polymer, wherein said water soluble polymer contains an aminooxy group and an oxime linkage is formed between the oxidized carbohydrate moiety and the aminooxy group on the water soluble polymer or wherein said water soluble polymer contains a hydrazide group and a hydrazone linkage is formed between the oxidized carbohydrate moiety and the hydrazide group on the water soluble polymer. Conjugates of aminooxy- or hydrazide-water soluble polymer, such as PSA and mPSA, are thus obtained in which the PSA or mPSA is attached via a carbohydrate moiety.

Abstract: Cell-based therapies for cartilage repair and regeneration selectively using chondrocytes carrying a CD24 surface marker are disclosed. In particular, chondrocytes carrying the CD24 surface marker have a high regenerative potential and low responsiveness to inflammatory cues. Since cartilage injuries as well as chronic cartilage degenerative conditions are often accompanied by a heightened inflammatory environment, cartilage cell populations carrying the CD24 surface marker, which are resistant to the inflammatory environment, provide more efficient cartilage repair. Thus, chondrocytes carrying the CD24 surface marker are useful in cell-based therapies for regenerating or repairing cartilage.

Abstract: Intravenous ferumoxytol is used to effectively label mesenchymal stem cells (MSCs) in vivo and is used for in vivo tracking of stem cell transplants with magnetic resonance (MR) imaging. The method eliminates risk of contamination and biologic alteration of MSCs associated with ex-vivo-labeling procedures.

Abstract: The present disclosure relates to a compound and method of using such compound, preferably in the form of a dietary supplement that, when administered, is capable of treating thyroid disease and various thyroid-related disorders, such as Hashimoto's thyroiditis. The unique combination of the composition is preferably administered orally via acid resistant or enteric-coated capsule, soft gel or tablet. The composition is preferably comprised of at least Catalase, Reduced Glutathione, Acetylated Glutathione, DPP-IV protease enzyme, Pepsin, Bromelain, Pancreatin, Vitamin D, and Selenium. The composition can further comprise a palliative agent, and can be provided in specific dosages or administered in forms besides those listed above.

Abstract: A single-cell-based electromechanical method for cancerous state detection including the steps of preparing a suspension of individually suspended biological cells, extracting a single cell from the suspension, holding the extracted single cell from the suspension, measuring a first electrical response of the held single cell, step-wised mechanical aspirating the held single cell to form a mechanically deformed cell, and measuring an electrical response of the held single cell after each step of mechanical aspirating. The cancerous state of the single cell is determined based on the changes in the measured electrical responses.

Abstract: Disclosed herein is a process for recycling water in a cellulosic conversion process that comprises selecting process stream(s) that may contain reduced levels of inhibitors and subsequently subjecting the stream(s) to a treatment process to produce treated water. The treated water is thereafter recycled to the cellulosic conversion process, associated utilities or the seal water system. The process streams selected for treatment may comprise less than 5 wt % organic content and/or less than 5 wt % inorganic content. The process of the invention comprises at segregating process streams based on their treatment requirements. The segregated process streams may be sent to respective separate treatments selected from anaerobic digestion, aerobic digestion, physical separation and chemical treatment.

Abstract: The present invention describes a method for the treatment of lignocellulosic material which method comprises contacting said lignocellulosic material with a composition comprising two or more enzyme activities, said enzyme activities being cellulase and/or hemicellulase activities, wherein the pH during the treatment is about 4.5 or lower, and the treatment is carried out at a dry matter content of 15% or more.

Abstract: A tissue sample that has been removed from a subject can be evaluated. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The monitoring can detect movement of fluid with the sample and cross-linking. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy.