Abstract: In a method for cultivating cells, there is provided a container (4) comprising an at least partly flexible container wall structure (41). The container is at least resting on a support structure (2). Into the container's interior space (42) there is introduced a gas, a liquid medium (5) and a cell culture. At least part of said support structure together with the container thus resting thereon are being moved, thereby inducing motion to the liquid medium in the container. An upwardly facing outer surface structure of said support structure comprises an elongate downwardly recessed portion (24, 25, 26) which causes a gutter (7) in the container's bottom wall structure (43).
Type:
Grant
Filed:
January 4, 2012
Date of Patent:
June 5, 2018
Assignee:
Strix Beheer B.V.
Inventors:
Nicolaas Marius Gerard Oosterhuis, Anthonie Tromper
Abstract: A bacterial composition for the treatment of colibacillosis on farms, in particular poultry farms, contains at least 105 cfu/ml of at least one of the following four strains of Bacillus subtilis or of Bacillus licheniformis: NOL01, NOL02, NOL03 and NOL04, and at least 105 cfu/ml of at least one lactic acid bacterium chosen from the following lactic acid bacteria: Lactococcus lactis spp lactis 1 strain NOL11 and Pediococcus pentosaceus 2 strain NOL12.
Abstract: The present invention relates generally to methods and pharmaceutical compositions for rejuvenating hematopoietic stem cells and progenitor cells from blood, intestinal tissue and dermal tissue by administration of at least one inhibitor of a GT-Pase, such as Cdc42 GTPase.
Type:
Grant
Filed:
April 30, 2013
Date of Patent:
May 29, 2018
Assignees:
CHILDREN'S HOSPITAL MEDICAL CENTER, UNIVERSITAET ULM
Inventors:
Hartmut Geiger, Maria Carolina Florian, Yi Zheng
Abstract: Provided is a biomass hydrothermal decomposition system which includes: a biomass supply unit that supplies a biomass raw material; hydrothermally decomposing the biomass raw material using pressurized hot water; a hydrothermal decomposition unit that dissolves lignin components and hemicellulose components in the pressurized hot water; a biomass solid fraction discharge unit that discharges a biomass solid fraction from the hydrothermal decomposition unit; an enzymatic liquefaction tank that is in connection with the biomass solid fraction discharge unit, and in which the discharged biomass solid fraction is introduced, and an enzyme is supplied to the biomass solid fraction to liquefy the biomass solid fraction; and a discharge unit that discharges the liquefied biomass solid fraction.
Type:
Grant
Filed:
July 11, 2012
Date of Patent:
May 29, 2018
Assignee:
MITSUBISHI HITACHI POWER SYSTEMS ENVIRONMENTAL SOLUTIONS, LTD.
Abstract: A method of manufacturing a nanopatterned biopolymer optical device includes providing a biopolymer, processing the biopolymer to yield a biopolymer matrix solution, providing a substrate with a nanopatterned surface, casting the biopolymer matrix solution on the nanopatterned surface of the substrate, and drying the biopolymer matrix solution to form a solidified biopolymer film on the substrate, where the solidified biopolymer film is formed with a surface having a nanopattern thereon. In another embodiment, the method also includes annealing the solidified biopolymer film. A nanopatterned biopolymer optical device includes a solidified biopolymer film with a surface having a nanopattern is also provided.
Type:
Grant
Filed:
November 5, 2007
Date of Patent:
May 15, 2018
Assignee:
Trustees of Tufts College
Inventors:
David Kaplan, Fiorenzo Omenetto, Brian Lawrence, Mark Cronin-Golomb
Abstract: The current invention concerns a method for obtaining a cellular composition comprising epithelial stem cells (EpSCs) from mammalian skin, whereby said composition comprises at least 90% of viable EpSCs, comprising the steps of: —obtaining a mammalian skin sample; —obtaining a cell suspension from said skin sample by performing at least one enzymatic dissociation step; and —culturing said cell suspension under low-attachment conditions. Preferably cellular composition comprises epithelial stem cells derived from the epidermal layer. In a second aspect, the current invention provides for a cellular composition obtained by the method according to the invention.
Abstract: The present technology pertains to methods and microbial co-cultures for converting lignocellulosic biomass to biofuels and/or other carbon-based chemicals. Aspects of the present disclosure relate to novel consolidated bioprocessing (CBP) methods by which the efficiency of the production of biofuels and/or other carbon-based chemicals from cellulosic biomass-containing materials can be increased. In particular, the present disclosure provides numerous microbiological co-cultures for increasing the efficiency of ethanol and/or lactic acid production from biomass.
Abstract: A process for removing sulphide from an aqueous solution comprising sulphide is disclosed, in which the aqueous solution is subjected to sulphide-oxidizing bacteria in the presence of oxygen in a reactor to oxidize sulphide to elemental sulphur. According to the process, a molecular-oxygen containing gas is supplied to a reactor containing the sulphide-oxidizing bacteria in an aqueous medium, such that one or more aerated zones and one or more non-aerated zones are created in the aqueous medium with upward liquid flow in the aerated zones and downward liquid flow in the non-aerated zones; and a feed stream of the aqueous solution comprising sulphide is injected into the reactor in the one or more non-aerated zones, wherein the one or more aerated zone(s) are not separated from the one or more non-aerated zone(s) by means of vertically extending reactor internal.
Type:
Grant
Filed:
September 26, 2014
Date of Patent:
April 17, 2018
Assignee:
Paques I.P. B.V.
Inventors:
Erik Van Zessen, Antonius Johannes Jorna, Sjoerd Hubertus Jozef Vellinga, Hendrik Dijkman
Abstract: This disclosure relates to inexpensive and efficient methods of preparing egg white (e.g., obtained from eggs laid by transgenic chickens) for bulk chromatographic isolation of proteins (e.g., recombinant proteins) from the egg white, as well as methods of filtering acidified egg white and methods of isolating proteins from the egg white.
Type:
Grant
Filed:
April 21, 2015
Date of Patent:
April 10, 2018
Assignee:
ALEXION PHARMACEUTICALS, INC.
Inventors:
Liang Chen, Markley C. Leavitt, Michael Titus
Abstract: The present invention concerns a cell-enriched fat graft and methods of making and using the same. The present invention also provides a kit for making and using the cell-enriched fat graft of invention.
Abstract: The invention relates to the compounds of formula I and pharmaceutically acceptable salts and esters thereof, wherein R1-R11 are as defined in the description and claims. In addition, the present invention relates to methods of manufacturing and using the compounds of formula I as well as pharmaceutical compositions containing such compounds. The compounds of formula I are useful in differentiating stem cells into more mature or adult-like hepatocytes for use as drug screening platforms and in disease modeling applications.
Type:
Grant
Filed:
March 12, 2014
Date of Patent:
May 10, 2016
Assignee:
Hoffmann-La Roche Inc.
Inventors:
Eric Chiao, Matthew Michael Hamilton, Sei Kameoka, Brian Leonard, Miriam Triyatni