Abstract: The present invention relates to assays, including amplification assays, conducted in the presence of modulators. These assays can be used to detect the presence of particular nucleic acid sequences. In particular, these assays can allow for genotyping or other genetic analysis.
Type:
Grant
Filed:
December 18, 2018
Date of Patent:
September 6, 2022
Assignee:
CALIFORNIA INSTITUTE OF TECHNOLOGY
Inventors:
Rustem F. Ismagilov, Bing Sun, Jesus Rodriguez Manzano, Eugenia Khorosheva, Matthew S. Curtis
Abstract: Provided herein is technology relating to methods for assaying a cell-containing body sample and particularly, but not exclusively, to treating a sample under conditions to cause cell lysis, preferably by means of a detergent; and subjecting the lysed sample to conditions causing the cleavage of nucleic acid molecules. The technology additionally relates to using nucleic acid cleavage conditions to enhance a membrane assay, a device for carrying out such an assay, and a kit for use in the assay.
Type:
Grant
Filed:
January 10, 2018
Date of Patent:
August 30, 2022
Assignee:
ABBOTT RAPID DIAGNOSTICS INTERNATIONAL UNLIMITED COMPANY
Inventors:
Jostein Holtlund, Andrew Campbell, Stig Morten Borch
Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.
Type:
Grant
Filed:
July 23, 2019
Date of Patent:
August 23, 2022
Assignee:
GENETICS RESEARCH, LLC
Inventors:
Anthony P. Shuber, William Glover, Thomas C. Meyers
Abstract: A system for nucleic acid sequencing includes a machine-readable memory and a processor configured to execute machine-readable instructions. The instructions, when executed by the processor, cause the system to expose template polynucleotide strands in a plurality of defined spaces of a sensor array to a series of flows of nucleotide species, the series comprising a sequence of random flows; and obtain, for each of the series of flows of nucleotide species, a signal indicative of how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands.
Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.
Abstract: Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to methods, compositions, and systems for sequencing a nucleic acid using one or more labels and signal amplitude to distinguish bases.
Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
Type:
Grant
Filed:
May 7, 2021
Date of Patent:
May 31, 2022
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.
Type:
Grant
Filed:
July 30, 2018
Date of Patent:
May 31, 2022
Assignee:
Bio-Rad Laboratories, Inc.
Inventors:
Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou
Abstract: A leukemia diagnostic kit may be an RT-PCR kit enabling expression levels of prohibitin-1 and prohibitin-2 to be checked in a leukemia patient specimen, wherein the accuracy and the reproducibility of leukemia diagnosis of the kit are higher than those of a conventional RT-PCR kit, thereby being useful as a kit for diagnosing leukemia, examining residual lesions and evaluating therapeutic effects.
Type:
Grant
Filed:
February 21, 2017
Date of Patent:
May 24, 2022
Assignee:
INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
Inventors:
Myung Guen Shin, Hye Ran Kim, Yong Gwan Won
Abstract: The present invention features novel, diverse, hybrid and engineered recombinase enzymes, and the utility of such proteins with associated recombination factors for carrying out DNA amplification assays. The present invention also features different recombinase ‘systems’ having distinct biochemical activities in DNA amplification assays, and differing requirements for loading factors, single-stranded DNA binding proteins (SSBs), and the quantity of crowding agent employed.
Type:
Grant
Filed:
October 9, 2018
Date of Patent:
May 24, 2022
Assignee:
ABBOTT DIAGNOSTICS SCARBOROUGH, INC.
Inventors:
Olaf Piepenburg, Niall A. Armes, Matthew James David Parker
Abstract: Disclosed herein are adapter nucleic acid sequences, double-stranded complexed nucleic acids, compositions, and methods for sequencing a double-stranded target nucleic acid with applications to error correction by duplex sequencing.
Type:
Grant
Filed:
December 8, 2016
Date of Patent:
May 17, 2022
Assignee:
TwinStrand Biosciences, Inc.
Inventors:
Michael W. Schmitt, Lawrence A. Loeb, Jesse J. Salk
Abstract: Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5? of the target-specific primer sequence; a tag sequence 5? of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.
Abstract: Methods of preparing a plurality of sample-barcoded anchor-domain-flanked gene specific deoxyribonucleic acid (DNA) fragments from a template nucleic acid, e.g., ribonucleic acid (RNA), sample are provided. Aspects of the methods include employing a set of gene specific primer pairs, wherein each pair of gene specific primers is made up of a forward primer and a reverse primer, at least one of which includes a sample barcode domain. The methods find use in a variety of different applications, including high-throughput sequencing, e.g., expression profiling, applications, including of small biological samples, e.g., single-cells.
Type:
Grant
Filed:
August 16, 2019
Date of Patent:
March 15, 2022
Assignee:
Cellecta, Inc.
Inventors:
Alex Chenchik, Costa Frangou, Mikhail Makhanov, Russell Paul Darst, IV, Donato Tedesco, Lester Kobzik
Abstract: Disclosed are methods and kits for endonuclease-assisted DNA amplification reaction using decontaminated primer solutions that are pre-treated with a nuclease. Nucleic acid amplification assays that employ nuclease-resistant, inosine-containing primers, endonuclease V enzymes to introduce a nick into a target DNA comprising at least one inosine, and a DNA polymerase to generate amplicons of a target DNA are also disclosed.
Type:
Grant
Filed:
March 30, 2018
Date of Patent:
March 8, 2022
Assignee:
GLOBAL LIFE SCIENCES SOLUTIONS OPERATIONS UK LTD
Inventors:
Robert Scott Duthie, John Richard Nelson, Anuradha Sekher
Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
Type:
Grant
Filed:
October 7, 2019
Date of Patent:
December 14, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
Abstract: The present invention is directed to methods, compositions and reaction mixtures for multiplexing COLD-PCR/ice-COLD-PCR to enrich simultaneously several low abundance alleles (mutant target sequences) from a sample. The invention also involves COLD-PCR/ice-COLD-PCR amplification performed on DNA fragments that have different melting temperatures, and therefore different critical denaturation temperatures, in a graded temperature approach such that mutation enrichment is achieved on all diverse DNA fragments simultaneously (temperature-independent COLD-PCR or TI-COLD-PCR). The invention also involves methods for enabling identification of variant-sequence alleles in the presence of a large excess of non-variant alleles in nucleic acids without the complication of polymerase-introduced errors or other primer-introduced artifacts.
Abstract: The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
Type:
Grant
Filed:
September 15, 2017
Date of Patent:
June 15, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
Abstract: The present invention describes methods for performing higher multiplexed PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.
Type:
Grant
Filed:
September 15, 2017
Date of Patent:
June 8, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Igor Kozlov, Amar Gupta, Randall Saiki, Alison Tsan
Abstract: Provided are methods for multiplex polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci that can be used to rapidly generate a highly specific STR profile from target nucleic acids. The resulting STR profiles are useful for human identification purposes in law enforcement, homeland security, military, intelligence, and paternity testing applications.
Type:
Grant
Filed:
October 16, 2017
Date of Patent:
June 1, 2021
Assignee:
ANDE CORPORATION
Inventors:
James W. Schumm, Richard F. Selden, Eugene Tan
Abstract: A method of making optically pure preparations of chiral ?PNA (gamma peptide nucleic acid) monomers is provided. Nano structures comprising chiral ?PNA structures also are provided. Methods of amplifying and detecting specific nucleic acids, including in situ methods are provided as well as compositions and kits useful in those methods. Lastly, methods of converting nucleobase sequences from right-handed helical PNA, nucleic acid and nucleic acid analog structures to left-handed ?PNA, and vice-versa, are provided.
Type:
Grant
Filed:
May 8, 2015
Date of Patent:
December 1, 2020
Assignee:
Carnegie Mellon University
Inventors:
Danith H. Ly, Wei-Che Hsieh, Iulia Sacui, Arunava Manna