Patents Examined by Susan Hanley
  • Inactivation method
    Patent number: 9273304
    Abstract: The present invention relates to the field of thermostable enzymes, methods for their inactivation, and related uses, kits and reagents. In particular, the invention relates to a method for reversible inactivation of thermophilic enzymes by chemical modification under aqueous or non-aqueous conditions.
    Type: Grant
    Filed: January 5, 2007
    Date of Patent: March 1, 2016
    Assignee: Thermo Fisher Scientific Baltics UAB
    Inventors: Arunas Lagunavicius, Daiva Tauraite, Jurgita Barkauskaite, Leonas Grinius
  • Methods and kits for obtaining a metabolic profile of living animal cells
    Patent number: 9274101
    Abstract: The present invention relates to growing and testing eukaryotic cells (e.g., animal or plant cells) in a multi-test format. In particular, the present invention provides methods and kits for obtaining a complex metabolic profile of animal cells. In addition, the present invention provides tools for assaying the effects of candidate compounds (e.g., hormones) on substrate utilization by mammalian cells.
    Type: Grant
    Filed: May 5, 2006
    Date of Patent: March 1, 2016
    Assignee: BIOLOG, Inc.
    Inventors: Barry Bochner, Larry Wiater
  • Method for production of L-amino acid
    Patent number: 9273332
    Abstract: The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate.
    Type: Grant
    Filed: October 11, 2007
    Date of Patent: March 1, 2016
    Assignee: Kaneka Corporation
    Inventors: Hiroyuki Kanamaru, Makoto Ueda, Hirokazu Nanba
  • Process for inhibiting microorganisms
    Patent number: 9253988
    Abstract: The invention relates to the use of Bacillus licheniformis strain OBT618 (ATCC PTA-122188) for inhibiting Clostridium pathogens in a material such as water or manure contaminated with the pathogen. The strain can be added to drinking water, animal feed or animal litter to reduce disease caused by the presence of Clostridium in an animal.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: February 9, 2016
    Assignee: Osprey Biotechnics, Inc.
    Inventors: Christopher J. Reuter, Steven J. MacKenzie, Lauren G. Danielson, Vincent Scuilla
  • Synbiotic mixture
    Patent number: 9254303
    Abstract: This invention relates to a preparation comprising N-acetyl-lactosamine and/or an oligosaccharide containing N-acetyl-lactosamine and a probiotic Lactobacillus sp. The invention extends to nutritional compositions comprising said preparation and to the use of the preparation in the prevention and treatment of pathogenic infections of the gastro-intestinal and upper respiratory tracts.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: February 9, 2016
    Assignee: Nestec S.A.
    Inventor: Norbert Sprenger
  • Aldose-ketose transformation for separation and/or chemical conversion of C6 and C5 sugars from biomass materials
    Patent number: 9242222
    Abstract: Systems for converting aldose sugars to ketose sugars and separating and/or concentrating these sugars using differences in the sugars' abilities to bind to specific affinity ligands are described.
    Type: Grant
    Filed: April 19, 2011
    Date of Patent: January 26, 2016
    Assignee: The University of Toledo
    Inventors: Sasidhar Varanasi, Patricia Relue, Bin Li
  • Hematopoietic stem cell identification probe
    Patent number: 9228952
    Abstract: The present invention provides a hematopoietic stem cell identification probe.
    Type: Grant
    Filed: July 10, 2013
    Date of Patent: January 5, 2016
    Assignee: Canon Kabushiki Kaisha
    Inventors: Kohei Watanabe, Tsuyoshi Nomoto, Mie Okano, Taichi Shintou, Takeshi Miyazaki, Yasuhiko Tabata, Hiroshi Kohara
  • Reagent and reagent kit for analysis of immature leukocyte
    Patent number: 9222934
    Abstract: The present invention provides a reagent for analysis of immature leukocytes comprising: a surfactant which can damage cell membranes of erythrocytes and mature leukocytes, a solubilizing agent which can shrink the damaged blood cells and at least one dye for staining nucleic acid selected from the dyes of the formulae (I) and (II), wherein X? is an anion.
    Type: Grant
    Filed: August 1, 2013
    Date of Patent: December 29, 2015
    Assignee: SYSMEX CORPORATION
    Inventors: Yukiko Kataoka, Tomohiro Tsuji, Shinichiro Oguni, Ayumu Yoshida, Masaki Abe
  • Microorganism comprising particles and uses of same
    Patent number: 9216158
    Abstract: A particle is disclosed. The particle comprising: (i) at least one inner core which comprises a solid matrix of nutrients for microorganism growth; (ii) an inner membrane being fabricated from a water-soluble polymer, the inner membrane surrounding the inner core and a population of dried microorganisms; and (iii) an outer porous membrane surrounding the inner membrane, the outer porous membrane being insoluble in water. Methods of generating same, propagating microorganisms within and uses of same are also disclosed.
    Type: Grant
    Filed: February 2, 2014
    Date of Patent: December 22, 2015
    Inventor: Ofir Menashe
  • Fluorescent probes for reactive sulfur species
    Patent number: 9212189
    Abstract: The invention provides reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells.
    Type: Grant
    Filed: June 11, 2012
    Date of Patent: December 15, 2015
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Christopher J. Chang, Alexander R. Lippert, Vivian S. Lin
  • Ceramide derivative and Golgi apparatus-labeling fluorescent probe using same
    Patent number: 9207232
    Abstract: A measure for selective fluorescent labeling of the Golgi apparatus, which has low cytotoxicity and excellent intracellular stability, is provided. According to the present invention, provided are a ceramide derivative represented by the following Chemical formula 1: in the formula, R1 is an alkyl group having 1 to 12 carbon atoms, R2 is an alkyl group having 1 to 20 carbon atoms, and n is an integer of 1 to 20, and a Golgi apparatus-labeling fluorescent probe using the same.
    Type: Grant
    Filed: August 13, 2012
    Date of Patent: December 8, 2015
    Assignee: National University Corporation Chiba University
    Inventors: Atsushi Nishida, Hiroyuki Nakamura, Tomohiko Makiyama, Toshihiko Murayama
  • Bimodal star polymer architectures as fluorescent and MRI imaging reagents
    Patent number: 9198985
    Abstract: Disclosed are star polymers comprising a polymeric body having a core with a site-isolated chromophore and a plurality of polymer chains emanating from the core; and at least one chelating moiety bonded to at least one polymer chain. Also disclosed are bimodal contrast agents derived from star polymers and further comprising at least one metal chelated by the at least one chelating moiety. Also disclosed are methods of making and using same. Also disclosed are imaging methods employing the disclosed star polymers and/or bimodal contrast agents. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
    Type: Grant
    Filed: May 21, 2010
    Date of Patent: December 1, 2015
    Assignee: Vanderbuilt University
    Inventor: Eva M. Harth
  • Methods for tumor diagnosis and therapy
    Patent number: 9186425
    Abstract: The present invention discloses a method for the enzyme-mediated, site-specific, in-vivo precipitation of a water soluble molecule in an animal. The enzyme is either unique to tumor cells, or is produced within a specific site (e.g., tumor) at concentrations that are higher than that in normal tissues. Alternatively, the enzyme is conjugated to a targeting moiety such as an antibody or a receptor-binding molecule.
    Type: Grant
    Filed: December 9, 2013
    Date of Patent: November 17, 2015
    Assignee: President and Fellows of Harvard College
    Inventors: Amin I. Kassis, Ravi S. Harapanhalli
  • Separation of glycans by mixed-mode liquid chromatography
    Patent number: 9169331
    Abstract: An exemplary multimodal chromatographic medium of the invention includes one or more strong anion exchange, weak anion exchange, strong cation exchange and/or weak cation exchange binding sites in combination with one or more reverse phase and/or hydrophilic interaction chromatography binding site. In an exemplary embodiment, the sites interact with one or more glycans in a mixture of glycans in a manner that allows separation of glycans in the mixture and analysis of the glycan mixture. The media are incorporated into devices and systems for chromatographic analysis. Also provided are methods of using the multimodal media of the invention to analyze glycans.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: October 27, 2015
    Assignee: DIONEX CORPORATION
    Inventors: Xiaodong Liu, Udayanath Aich, Christopher A. Pohl
  • Biosynthesis of CMP-legionaminic acid from fructose-6-P, and respective pathway intermediates, using novel GDP-linked precursors
    Patent number: 9157105
    Abstract: The sialic acid-like sugar legionaminic acid is found as a virulence-associated surface glyco-conjugate in Legionella pneumophila and Campylobacter coli. In this work, we have purified and biochemically characterized eleven candidate biosynthetic enzymes from C. jejuni, thereby fully reconstituting the biosynthesis of legionaminic acid and its CMP-activated form, starting from fructose-6-P. This pathway involves unique GDP-linked intermediates and provides a facile means for the efficient large-scale synthesis of an important sialic acid mimic and novel precursors.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: October 13, 2015
    Assignee: National Research Council of Canada
    Inventors: Ian C. Schoenhofen, Susan M. Logan
  • Shikimate pathway inhibitors and the use thereof
    Patent number: 9145374
    Abstract: The present invention relates to methods of inhibiting shikimate pathway, comprising administering to a subject a pharmaceutically acceptable composition comprising a compound having a formula: or pharmaceutically acceptable salts thereof.
    Type: Grant
    Filed: January 10, 2014
    Date of Patent: September 29, 2015
    Assignee: NATIONAL TSING HUA UNIVERSITY
    Inventors: Wen-Ching Wang, Shih-Ching Chou, Ming-Hua Hsu
  • Synthesis of sialooligosaccharide derivatives
    Patent number: 9102966
    Abstract: The invention relates to a method for the synthesis of compounds of general formula (1A) and salts thereof wherein one of the R groups is an ?-sialyl moiety and the other is H, X1 represents a carbohydrate linker, A is a D-glucopyranosyl unit optionally substituted with fucosyl, R1 is a protecting group that is removable by hydrogenolysis, the integer m is 0 or 1, by a transsialidation reaction.
    Type: Grant
    Filed: July 15, 2011
    Date of Patent: August 11, 2015
    Assignee: GLYCOM A/S
    Inventors: Andreas Schroven, Elise Champion, Gyula Dekany, Christoph Röhrig, Ioannis Vrasidas, Ignacio Figueroa Pérez, Markus Hederos, Julien Boutet, Ágnes Ágoston, Piroska Kovács-Pénzes, Ferenc Horváth, Christian Risinger, Gergely Pipa, Sándor Demkó, Lars Kröger
  • Detection of bacteria and fungi
    Patent number: 9096884
    Abstract: A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described.
    Type: Grant
    Filed: April 19, 2010
    Date of Patent: August 4, 2015
    Assignee: MOMENTUM BIOSCIENCE LTD
    Inventors: Stuart Wilson, William Mullen
  • Sulfite pretreatment for biorefining biomass
    Patent number: 9090915
    Abstract: The present invention relates to a method using sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL). More specifically, it relates to a sulfite-based chemical process for pretreating biomass in solutions to reduce access barriers of enzymes to the lignocellulose, resulting in efficient conversion through enzymatic saccharification.
    Type: Grant
    Filed: April 17, 2009
    Date of Patent: July 28, 2015
    Assignees: Wisconsin Alumni Research Foundation, The United States of America as represented by the Secretary of Agriculture
    Inventors: Gaosheng Wang, Xuejun Pan, Jun Yong Zhu, Roland L. Gleisner
  • Process for the production of polyamines
    Patent number: 9085786
    Abstract: The present invention relates to a process for the production of a polyamine involving the use of enzymes; in particular to a process performed in aqueous environment; to the polyamines produced by said method; as well as the use of said polyamines for manufacturing paper, for immobilizing enzymes, or for preparing pharmaceutical or cosmetical compositions. The invention also relates to a novel method for in situ regeneration of cofactors NAD(P)+.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: July 21, 2015
    Assignee: BASF SE
    Inventors: Bernhard Hauer, David Karim Engelmark Cassimjee, Per Berglund