Patents Examined by Tekchand Saidha
  • Patent number: 11319543
    Abstract: The present invention relates to a yeast cell that is genetically modified comprising: a) a disruption of one or more aldehyde dehydrogenase (E.C:1.2.1.4) native to the yeast; b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.
    Type: Grant
    Filed: May 18, 2016
    Date of Patent: May 3, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Paul Klaassen, Jozef Petrus Johannes Schmitz, Paulus Petrus De Waal, Arjen Bouma
  • Patent number: 11319562
    Abstract: Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse ?-oxidation cycle.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: May 3, 2022
    Inventors: Ramon Gonzalez, James M. Clomburg
  • Patent number: 11312946
    Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
    Type: Grant
    Filed: May 28, 2019
    Date of Patent: April 26, 2022
    Assignee: NOVOZYMES A/S
    Inventors: Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Gylstorff, Jesper Vind, Christian Jackel
  • Patent number: 11304892
    Abstract: Alteromonas macleodii Dow02-2 is provided, wherein the Alteromonas macleodii Dow02-2 was deposited at German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen and Zellkulturen, DSMZ) under the accession number DSM33380, and its metabolites comprise 2-hydroxy-3-methylbutyric acid. A method for providing 2-hydroxy-3-methylbutyric acid is also provided, wherein the method comprises isolating the 2-hydroxy-3-methylbutyric acid from metabolites of Alteromonas macleodii Dow02-2 of biologically pure culture. A method for at least one of increasing expression of TGM1 gene, increasing expression of KRT1 gene, increasing expression of KRT10 gene, increasing expression of KRT14 gene, increasing expression of FLG gene, increasing expression of GBA gene, and increasing expression of HAS3 gene is also provided, wherein the method comprises administering to a subject in need thereof an effective amount of 2-hydroxy-3-methylbutyric acid.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: April 19, 2022
    Assignee: TCI Co., Ltd.
    Inventors: Chu-Han Huang, Huan-You Lin, Cheng-Yu Ho, Yung-Hsiang Lin
  • Patent number: 11306278
    Abstract: A cleaning agent for hard surfaces and methods for cleaning are provided herein. In one embodiment, the cleaning agent includes at least one first amylase, wherein the first amylase is an ?-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof. The cleaning agent further includes at least one second amylase, wherein the second amylase is an AA560 ?-amylase from Bacillus sp. or a functional fragment or a variant thereof. In another embodiment, the method includes dispensing the cleaning agent into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.
    Type: Grant
    Filed: July 12, 2019
    Date of Patent: April 19, 2022
    Assignee: HENKEL AG & CO. KGAA
    Inventors: Thomas Eiting, Nina Mussmann, Thorsten Bastigkeit, Inga Kerstin Vockenroth, Noelle Wrubbel, Thomas Weber, Silke Menke
  • Patent number: 11306338
    Abstract: Disclosed is a new tagatose 6-phosphate 4-epimerase, which is capable of converting fructose 6-phosphate into tagatose 6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: April 19, 2022
    Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Yanhe Ma, Yuanxia Sun, Yunjie Li, Jiangang Yang
  • Patent number: 11297862
    Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: April 12, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Viktor Marius Boer, Priscilla Zwartjens, Eric Van Den Berg
  • Patent number: 11293043
    Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto.
    Type: Grant
    Filed: April 10, 2020
    Date of Patent: April 5, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Viktor Marius Boer, Priscilla Zwartjens, Eric Van Den Berg
  • Patent number: 11293042
    Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.
    Type: Grant
    Filed: July 13, 2020
    Date of Patent: April 5, 2022
    Assignee: GLYCOM A/S
    Inventors: Margit Pedersen, Manos Papadakis, Peter Becker, Eric Samain, Pauline Peltier-Pain, Katrine Bych, Ted Johanson, Elise Champion, Gyula Dekany
  • Patent number: 11286470
    Abstract: Methods of making mutant Cas9 proteins are described.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: March 29, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Alejandro Chavez, Frank J. Poelwijk, George M. Church
  • Patent number: 11279957
    Abstract: To provide a method for producing a polyester comprising at least a 3-hydroxybutyrate unit as a polymerization unit and having a high molecular weight and a narrow molecular weight distribution (that is, small Mw/Mn). A method for producing a polyester comprising culturing a microorganism in a culture solution containing a carbon source and a nitrogen source, the polyester having a weight average molecular weight of 1,000,000 or greater and comprising at least a 3-hydroxybutyrate unit as a polymerization unit. The culture conditions include maintenance of an osmotic pressure of the culture solution from 200 mOsm to 900 mOsm during culture period, and maintenance of a nitrogen atom concentration of the culture solution at 0.30 g/L or greater during culture period.
    Type: Grant
    Filed: August 28, 2018
    Date of Patent: March 22, 2022
    Assignee: MITSUBISHI GAS CHEMICAL COMPANY, INC.
    Inventor: Akira Maehara
  • Patent number: 11279958
    Abstract: A method of producing lipids, containing the steps of: culturing a transformant into which a gene encoding at least one of the proteins selected from the group consisting of the following proteins (A) to (C) is introduced; and producing fatty acids or lipids containing the same as components: (A) A protein consisting of the amino acid sequence of the 23rd to 146th amino acids set forth in SEQ ID NO: 1; (B) A protein consisting of an amino acid sequence having 70% or more identity with the amino acid sequence of the protein (A), and having acyl carrier protein activity; and (C) A protein containing the amino acid sequence of the protein (A) or (B), and having acyl carrier protein activity.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: March 22, 2022
    Assignee: Kao Corporation
    Inventor: Tatsuro Ozaki
  • Patent number: 11279710
    Abstract: The present disclosure provides solid forms, including a salt or co-crystal, of Compound I: which exhibits Acetyl-CoA carboxylase (“ACC”) inhibitory activity and may be useful in treating ACC mediated diseases. Also provided herein are processes or steps for the preparation of a Compound I and intermediates useful for the processes or steps described herein.
    Type: Grant
    Filed: July 9, 2020
    Date of Patent: March 22, 2022
    Assignee: Gilead Sciences, Inc.
    Inventors: Michael Geier, Luke Humphreys, Mark E. Scott
  • Patent number: 11274283
    Abstract: A method for the enzymatic conversion of a phenol substrate into a corresponding catechol product comprises the step of incubating the phenol substrate with a Ralstonia solanacearum tyrosinase enzyme, or a functional derivative thereof, in a reaction mixture, for a period of time sufficient to allow the enzyme convert at least some of the phenol substrate into the catechol product.
    Type: Grant
    Filed: September 23, 2020
    Date of Patent: March 15, 2022
    Assignee: University College Dublin, National University of Ireland, Dublin
    Inventors: Kevin O'Connor, Susan Molloy, Reeta Davis, Wesley Shaw
  • Patent number: 11261450
    Abstract: Provided is a recombinant cell that produces isoprene or terpene, wherein the recombinant cell includes an ability to synthesize isopentenyl diphosphate through a mevalonate pathway (MVA pathway), wherein the recombinant cell lacks an ability to synthesize isopentenyl diphosphate through an endogenous non-mevalonate pathway (MEP pathway), wherein the recombinant cell includes an isoprene synthase gene or a terpene synthase gene as a foreign gene, and wherein the recombinant cell produces, with the expression of the foreign gene, isoprene or terpene having 10, 15, 20, 30, or 40 carbon atoms. The mevalonate pathway is preferably an exogenous mevalonate pathway.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: March 1, 2022
    Assignee: SEKISUI CHEMICAL CO., LTD.
    Inventors: Kana Matsushima, Masahiro Furutani, Kazufumi Kawabata
  • Patent number: 11261477
    Abstract: In alternative embodiments, provided are methods for the glycosylation modification of bioactive compounds and drugs using isolated, recombinant or genetically modified uridine diphosphate glycosyl-transferases (UGTs). In alternative embodiments, provided are methods for modifying UGTs to generate recombinant UGTs with altered donor and/or acceptor specificities. In alternative embodiments, provided are methods for screening for recombinantly engineered UGTs with new or altered properties, for example, for new or altered donor and/or acceptor specificities, where in alternative embodiments the screening comprise use of bacterial, yeast or baculovirus expression system.
    Type: Grant
    Filed: July 8, 2020
    Date of Patent: March 1, 2022
    Assignee: UNIVERSITY OF NORTH TEXAS
    Inventor: Xiaoqiang Wang
  • Patent number: 11261218
    Abstract: The present invention relates to a species of genus Pseudomonas identified as Pseudomonas BR11571, termed Candidatus Pseudomonas metallosolvens, having Accession Deposit Number DSM 32538.
    Type: Grant
    Filed: September 17, 2018
    Date of Patent: March 1, 2022
    Assignee: BRAIN AG
    Inventors: Esther Gabor, Renate Schulze, Arnulf Kletzin
  • Patent number: 11254718
    Abstract: An expression vector is provided for production of human alpha-synuclein (?S) protein or a conservative variant thereof that exhibits a decreased tendency to self-aggregate in an ?S seed amplification assay (SAA). The expression vector comprises a nucleic acid sequence coding for human ?S protein or a conservative variant, the nucleic acid sequence comprising codons that have been optimized to produce human ?S protein or a conservative variant when expressed by a host cell such as E. coli. The codons have been optimized to avoid amino acid misincorporation in the expressed protein. Methods for purification of the expressed protein are also provided.
    Type: Grant
    Filed: May 18, 2021
    Date of Patent: February 22, 2022
    Assignee: Amprion, Inc.
    Inventors: Luis Concha, Carly Farris, Bret Holguin, Yihua Ma
  • Patent number: 11248220
    Abstract: The present disclosure relates to a novel acetohydroxy acid synthase, a microorganism comprising the same, or a method for producing an L-branched-chain amino acid using the same.
    Type: Grant
    Filed: April 8, 2021
    Date of Patent: February 15, 2022
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ae Ji Jeon, Byeong Cheol Song, Ji Hye Lee, Jong Hyun Kim, Hye Won Kim
  • Patent number: 11248194
    Abstract: Cleaning compositions comprising endo-?-1,3-glucanase enzyme and cleaning adjunct. Methods of treating surfaces including fabrics by contacting the surface with an aqueous was liquor having the cleaning composition therein. The compositions and methods are particularly for cleaning cotton fabrics. The compositions and methods are particularly for removal of soils containing callose, curdlan, pachyman, scleroglucan or schizophyllan. The compositions and methods are particularly for improving whiteness of a fabric, improved soil removal from a fabric, for malodour removal from a fabric, for anti-wrinkle benefits and/or for improved drying of a fabric.
    Type: Grant
    Filed: March 12, 2020
    Date of Patent: February 15, 2022
    Assignee: The Procter & Gamble Company
    Inventors: Neil Joseph Lant, Katherine Esther Latimer