Patents Examined by Tekchand Saidha
  • Patent number: 11685945
    Abstract: Compositions and methods are disclosed that provide a rapid, sensitive, and accurate cell-based assay for enzyme activity, particularly for enzyme activities associated with botulinum toxins. A cell is provided that expresses a construct that includes an anchor region, a cleavage site, and a reporting region having two or more identical reporter peptides. Enzymatic activity at the cleavage site releases the reporter region into the cytosol, where multiple degradation events occur. The observed change in the signal is proportional to the enzymatic activity.
    Type: Grant
    Filed: February 6, 2020
    Date of Patent: June 27, 2023
    Assignee: BioMadison, Inc.
    Inventor: Ward C Tucker
  • Patent number: 11680252
    Abstract: A PET hydrolase having improved thermal stability is disclosed. The PET hydrolase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 248 or a corresponding position with proline.
    Type: Grant
    Filed: November 22, 2021
    Date of Patent: June 20, 2023
    Assignee: HUBEI UNIVERSITY
    Inventors: Chun-Chi Chen, Jian-Wen Huang, Xiuqin Li, Wei Zeng, Lilan Zhang, Yunyun Yang, Longhai Dai, Jian Min, Yu Yang, Rey-Ting Guo
  • Patent number: 11680231
    Abstract: The present invention relates to polypeptides having hexosaminidase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: June 20, 2023
    Assignee: NOVOZYMES A/S
    Inventors: Christian Berg Oehlenschlaeger, Dorotea Raventos Segura, Rebecca Munk Vejborg, Henrik Marcus Geertz-Hansen, Lilian Eva Tang Baltsen, Jesper Salomon
  • Patent number: 11680270
    Abstract: Disclosed is a method of producing lactic acid using a recombinant acid-resistant yeast with inhibited lactate metabolism and alcohol production. More specifically, disclosed are a recombinant acid-resistant yeast in which lactate consumption reaction is reduced and which is imparted with lactic-acid-producing ability to thereby exhibit improved lactic-acid-producing ability and reduced ethanol production, and a method of producing lactic acid using the same.
    Type: Grant
    Filed: October 7, 2020
    Date of Patent: June 20, 2023
    Assignee: SK Innovation Co., Ltd.
    Inventors: Jae Yeon Park, Tae Young Lee, Ki Sung Lee
  • Patent number: 11680279
    Abstract: A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism that is able to produce the objective substance, which microorganism has been modified so that the activity of an enzyme involved in SAM cycle (SAM cycle enzyme) is increased.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: June 20, 2023
    Assignee: AJINOMOTO CO., INC.
    Inventors: Benjamin Mijts, Christine Roche, Rekha Murali, Sayaka Asari, Keita Fukui, Miku Toyazaki, Keiko Noguchi
  • Patent number: 11673921
    Abstract: Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from Vibrio species such as cellular extracts from Vibrio natriegens.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: June 13, 2023
    Assignee: Northwestern University
    Inventors: Michael Christopher Jewett, Benjamin James Des Soye, Samuel Ryan Davidson
  • Patent number: 11667905
    Abstract: The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG.
    Type: Grant
    Filed: December 14, 2021
    Date of Patent: June 6, 2023
    Assignee: HANSA BIOPHARMA AB
    Inventors: Christian Kjellman, Sofia Jarnum, Emma Nordahl
  • Patent number: 11667896
    Abstract: The present invention refers to a modified D-amino acid oxidase (DAAO). In particular, the modified DAAO of the present invention has the activity of catalyzing the oxidation of D-glufosinate into PPO. Further, the modified DAAO of the present invention has increased activity of catalyzing the oxidation of D-glufosinate into PPO and/or increased stability as compared to SEQ ID NO: 4. The present invention also refers to the polynucleotide encoding the modified DAAO of the present invention, the vector and host cell expressing the modified DAAO of the present invention, and the method of producing L-glufosinate with the modified DAAO and host cell of the present invention.
    Type: Grant
    Filed: June 9, 2022
    Date of Patent: June 6, 2023
    Assignee: Hunan Lier Biotech Co., LTD
    Inventors: Xinkai Xie, Wei Xu, Junying Fan
  • Patent number: 11660961
    Abstract: The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.
    Type: Grant
    Filed: October 9, 2020
    Date of Patent: May 30, 2023
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Taek Soon Lee, Aram Kang
  • Patent number: 11655275
    Abstract: Nucleases and methods of using these nucleases for alteration of a CFTR gene and generation of cells and animal models.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: May 23, 2023
    Assignee: Sangamo Therapeutics, Inc.
    Inventor: Anthony Conway
  • Patent number: 11655484
    Abstract: The present invention provides for a mechanism to completely replace the electron accepting function of glycerol formation with an alternative pathway to ethanol formation, thereby reducing glycerol production and increasing ethanol production. In some embodiments, the invention provides for a recombinant microorganism comprising a down-regulation in one or more native enzymes in the glycerol-production pathway. In some embodiments, the invention provides for a recombinant microorganism comprising an up-regulation in one or more enzymes in the ethanol-production pathway.
    Type: Grant
    Filed: November 13, 2020
    Date of Patent: May 23, 2023
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: Arthur J. Shaw, IV, Aaron Argyros, Trisha Barrett
  • Patent number: 11655489
    Abstract: This invention discloses a method for improving n-3 polyunsaturated fatty acid enrichment efficiency in glyceride, belonging to the field of deep processing of oil. The method of this invention includes providing oil, lipase and metal ionic liquid into a reactor. After a reaction time, a mixture is obtained and free fatty acid is removed from the mixture, and the product is the n-3 polyunsaturated fatty acid-rich glyceride. Compared with the traditional chemical method, the method of this invention is characterized by mild reaction conditions and producing lower level of by-products. Meanwhile, metal ions selected in the present invention are able to promote the hydrolysis of lipase, solving the problem of long reaction time in the conventional enzymatic method. Rapid hydrolysis is achieved by this method so as to realize enrichment of EPA and DHA in glyceride.
    Type: Grant
    Filed: August 25, 2021
    Date of Patent: May 23, 2023
    Assignee: Jiangnan University
    Inventors: Xiaosan Wang, Ye Chen, Cong Jiang, Zhuangzhuang Yang, Yifan Wang, Wenhua Jin
  • Patent number: 11655478
    Abstract: The present invention relates to a novel promoter for regulating ADH gene expression in an organic acid-resistant yeast, and a method of producing an organic acid by expressing an organic acid production-related gene using the same. When an organic acid production-related target gene is expressed in the organic acid-resistant yeast using the novel promoter according to the present invention, there is an advantage in that the yeast can produce the organic acid with high efficiency while having resistance to the organic acid without inhibiting the growth ability of the yeast.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: May 23, 2023
    Assignee: SK Innovation Co., Ltd.
    Inventors: Jae Yeon Park, Tae Young Lee, Ki Sung Lee, Outi Koivistoinen, Kari Koivuranta
  • Patent number: 11643674
    Abstract: Disclosed are methods for the pre-treatment of lignocellulose by adding an alkali or acid reagent(s) during the densification thereof, and for the biotransformation thereof. In the method, an alkali reagent(s) or acid reagent(s) is added to a lignocellulosic raw material for a densification pre-treatment to form an alkali- or acid-containing densified lignocellulose with a compressed compact shape, thereby achieving the pre-treatment. The acid or alkali in the pre-treated lignocellulose can further pre-treat the lignocellulosic raw material in a mild manner during the subsequent transportation and storage processes. If a subsequent pre-treatment is needed, then the severity thereof is reduced substantially; in addition, the uniform mixing of the acid or alkali with the lignocellulose and a large density of the raw material promote a high efficiency and a high loading capacity of the subsequent pre-treatment of the densified lignocellulose. The method is simple and efficient.
    Type: Grant
    Filed: July 17, 2019
    Date of Patent: May 9, 2023
    Assignee: NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Mingjie Jin, Xiangxue Chen, Xinchuan Yuan
  • Patent number: 11639512
    Abstract: Provided is a method for screening a Corynebacterium constitutive expression vector promoter on the basis of transcriptome sequencing; and further provided are the Corynebacterium constitutive expression vector promoter screened on the basis of transcriptome sequencing, an expression vector comprising the promoter, a recombination strain obtained by transforming a host cell Corynebacterium glutamicum using the expression vector, and applications thereof.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: May 2, 2023
    Inventors: Panpan Xing, Haixia Su, Jiong Wang, Xuechen Mei, Kun Wan, Mengjun Song, Jing Li, Aifu Liu
  • Patent number: 11634554
    Abstract: The invention relates to treatment of waste with one or more microorganisms for the purposes of, including but not limited to, degrading waste, bioremediation of waste, enhancing waste stabilization, reducing contaminants in waste, reducing odor in waste, reducing organics in waste, and combinations thereof. More particularly, the invention relates to isolated Bacillus strains, and strains having all of the identifying characteristics of these strains, and combinations thereof, for uses comprising the above-mentioned uses.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: April 25, 2023
    Assignee: Microbial Discovery Group, LLC
    Inventors: Michael R. King, Sona Son, Claire Heile
  • Patent number: 11634734
    Abstract: The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).
    Type: Grant
    Filed: February 8, 2021
    Date of Patent: April 25, 2023
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: Charles F. Rice, Ryan Skinner, Trisha Barrett, Aaron Argyros
  • Patent number: 11634698
    Abstract: Disclosed herein are polypeptides having nuclease activity. Some of the polypeptides having nuclease activity were generated by an improved gene site mutagenesis (“GSSM”) method or the tailored multi-site combinatorial assembly (“TMCA”) method. Also disclosed are compositions and kits comprising the polypeptides having nuclease activity, and methods for making and using these polypeptides, compositions and kits.
    Type: Grant
    Filed: December 10, 2018
    Date of Patent: April 25, 2023
    Assignee: BASF SE
    Inventors: Xuqiu Tan, Kenneth Barrett, Jared Dennis, Dongmei Ren, Corey Dodge, Bin Zhang
  • Patent number: 11634462
    Abstract: Methods of processing protein, methods of generating products with the processed protein, and products comprising and/or made with the processed protein. The methods of processing protein include hydrolyzing the protein with a proteolytic agent such as thermolysin to generate hydrolyzed peptides and, optionally, crosslinking the hydrolyzed peptides with a transglutaminase to generate crosslinked peptides. The methods reduce the allergenicity of allergenicity proteins such as ?-lactoglobulin and casein. The methods of generating products with the processed protein include methods of making foams, emulsions, and/or food products with the processed protein. The products comprising and/or made with the processed protein accordingly include foams, emulsions, and food products. The foams, emulsions, and food products have decreased allergenicity compared to corresponding products made with non-processed proteins.
    Type: Grant
    Filed: May 20, 2019
    Date of Patent: April 25, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventor: Srinivasan Damodaran
  • Patent number: 11629328
    Abstract: The present application relates to plant growth promoting microbes (PGPMs), compositions comprising these PGPMs and methods of using these PGPMs and/or compositions for enhancing plant health, plant growth and/or plant yield, and/or for preventing, inhibiting, or treating the development of plant pathogens or the development of phytopathogenic diseases. This application also provides non-naturally occurring plant varieties that are artificially infected with a PGPM descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.
    Type: Grant
    Filed: November 11, 2020
    Date of Patent: April 18, 2023
    Assignee: TAXON BIOSCIENCES INC.
    Inventors: Matthew N. Ashby, Christopher P. Belnap, Matthew Cornyn Kuchta, Victor Kunin, Caroline Kostecki, Ulrika Lidstrom, Natalia Shestakova, Ladonna Wood