Patents Examined by Tekchand Saidha
  • Systems and methods for co-culture of oxygen sensitive bacteria and yeast
    Patent number: 11732278
    Abstract: Methods, compositions, and systems for propagation and fermentation of co-cultures of an oxygen sensitive bacteria and a yeast, particularly large scale operations for production of a bioproduct are provided. Methods of co-culture provide growth sufficient to minimize growth of contaminating bacteria.
    Type: Grant
    Filed: March 10, 2021
    Date of Patent: August 22, 2023
    Assignee: POET Research, Inc.
    Inventors: Benjamin M. Gerdes, Kyle R. Larson
  • Cleaning compositions comprising dispersin variants
    Patent number: 11732220
    Abstract: Cleaning compositions may include novel dispersin variants having improved properties relative to the parent dispersin. Such properties may include one or more of the following; wash performance, stability e.g. detergent and/or storage stability. The cleaning compositions may be suitable for use in cleaning processes and include detergent compositions, such as laundry compositions and dish wash compositions, including hand wash and automatic dish wash compositions.
    Type: Grant
    Filed: March 29, 2021
    Date of Patent: August 22, 2023
    Assignee: Henkel AG & Co. KGaA
    Inventors: Mirko Weide, Susanne Wieland, Lars Henrik Oestergaard, Annette Helle Johansen, Steffen Danielsen, Roland Alexander Pache, Johanne Moerch Jensen, Frank Winther Rasmussen, Martin Gudmand, Kasper Damgaard Tidemand
  • Sirtuin 4 (SIRT4) lipoamidase activity and uses thereof
    Patent number: 11726088
    Abstract: The present application provides a method of assaying pyruvate dehydrogenase complex (PDHC) activity in a mammalian cell that expresses human sirtuin 4 (SIRT4) comprising measuring a level of a dihydrolipoyllysine acetyltransferase (DLAT) lipoamide peptide comprising the amino acid sequence TDK[lipoyl]AT in the cell. The present application also demonstrates that sirtuin 4 (SIRT4) acts as a cellular lipoamidase that negatively regulates pyruvate dehydrogenase complex (PDHC) activity through hydrolysis of its lipoamide cofactors.
    Type: Grant
    Filed: August 12, 2020
    Date of Patent: August 15, 2023
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Thomas Shenk, Todd M. Greco, Ileana M. Cristea, Rommel A. Mathias, Adam Oberstein
  • Biofixation of greenhouse gas by mass culture of sp. microalga in high efficiency medium
    Patent number: 11725219
    Abstract: The invention is directed to a culture medium for Haematococcus that contains combustion gases like carbon dioxide, carbon monoxide, and oxides of nitrogen or sulfur and which fixes the carbon, nitrogen or sulfur in these combustion gases into biomass and to methods providing superior biomass yields using this culture medium to culture select species of Haematococcus such as Haematococcus sp. KAU-01.
    Type: Grant
    Filed: December 7, 2020
    Date of Patent: August 15, 2023
    Assignee: King Abdulaziz University
    Inventors: Adnan Jaman Turki, Md. Abu Affan, Salim Marzoog Al-Harbi
  • Production of fusel lactates via biocatalysis
    Patent number: 11725220
    Abstract: Microbial enzymes are used for esterification of biomass-derived substrates for production of industrially valuable esters. E. coli was used as an efficient platform for recombinant synthesis of fusel lactates such as the green solvent ethyl lactate.
    Type: Grant
    Filed: August 26, 2020
    Date of Patent: August 15, 2023
    Assignees: National Technology & Engineering Solutions of Sandia, LLC, Arizona Board of Regents on Behalf of Arizona State University
    Inventors: Ryan Wesley Davis, Arul Mohzy Varman, Aditya Pandharinath Sarnaik, Amit Kumar Jha
  • Lipase and uses of the same
    Patent number: 11718837
    Abstract: To find a novel lipase suitable for processing of oils and fats and to use and apply the lipase. Provided are a novel lipase including an amino acid sequence having 90% or more identity with an amino acid sequence of SEQ ID NO: 1 or 2, and an enzyme preparation containing the same as an active ingredient. The enzyme preparation is used for modifying and improving the physical properties of oils and fats.
    Type: Grant
    Filed: July 29, 2020
    Date of Patent: August 8, 2023
    Assignee: AMANO ENZYME INC.
    Inventors: Tomohiro Kimura, Tomoya Ryoki, Yusuke Tanaka, Naoto Katsumata, Yukihide Sato
  • Fermentative production of oligosaccharides by total fermentation utilizing a mixed feedstock
    Patent number: 11713475
    Abstract: Disclosed are genetically engineered microbial cells for the production of oligosaccharides comprising a galactose-?1,4-glucose moiety at their reducing end, wherein said microbial cells are able to produce said oligosaccharides in the absence of exogenously added lactose, and a method of producing said oligosaccharides using said microbial cells.
    Type: Grant
    Filed: September 2, 2019
    Date of Patent: August 1, 2023
    Assignee: Chr. Hansen HMO GmbH
    Inventors: Stefan Jennewein, Dirk Wartenberg, Katja Parschat
  • Synthetic metabolic funneling for biochemical production
    Patent number: 11708590
    Abstract: Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: July 25, 2023
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: David Nielsen, Brian Thompson
  • Method of protein purification from
    Patent number: 11697803
    Abstract: The present disclosure provides methods for releasing intracellular proteins. The method allows isolation of the protein of interest from the cell without the requirement for mechanical disruption of the cells, without the need for isolation of the cells from the culture media, and without the need for removal of the cells from the culture media.
    Type: Grant
    Filed: July 15, 2019
    Date of Patent: July 11, 2023
    Assignee: Ansun Biopharma, Inc.
    Inventor: Stephen Hawley
  • Esterases and uses thereof
    Patent number: 11692181
    Abstract: The present invention relates to novel esterases, more particularly to esterase variants having improved activity and/or improved thermostability compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
    Type: Grant
    Filed: July 26, 2019
    Date of Patent: July 4, 2023
    Assignee: CARBIOS
    Inventors: Isabelle Andre, Vincent Tournier, Alexandre Gilles, Sophie Duquesne
  • Anaerobic fermentative production of furandicarboxylic acid
    Patent number: 11692210
    Abstract: The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.
    Type: Grant
    Filed: September 1, 2021
    Date of Patent: July 4, 2023
    Assignee: BRASKEM S.A.
    Inventors: Iuri Estrada Gouvea, Marcos Rogerio Simoes, Mariana Trovo Marchesin, Aline Silva Romão Dumaresq
  • Blood profiling with protease inhibitors
    Patent number: 11693006
    Abstract: The present disclosure relates to methods for generating blood protein profiles from whole blood, red blood cell enriched blood samples, or red blood cell components. The methods involve the comparison of protein levels before and after incubation with protease inhibitors, or the comparison of protein levels after incubation in the presence and absence of protease inhibitors. The protein profiles are used for monitoring and diagnosing diseases and disorders in subjects including colorectal cancer and preeclampsia.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: July 4, 2023
    Assignee: Sangui Bio Pty. Ltd
    Inventors: Benjamin Ross Herbert, Elisabeth Karsten
  • Halogenation of complex organic compounds
    Patent number: 11692176
    Abstract: The disclosure provides biocatalysts that halogenate complex chemical compounds in specific and predictable ways. Also disclosed are halogenated complex organic compounds. The disclosure further provides methods for the halogenation of complex chemical compounds and methods of inhibiting the contraction of smooth muscle in mammals.
    Type: Grant
    Filed: April 12, 2021
    Date of Patent: July 4, 2023
    Assignee: REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: David H. Sherman, Amy E. Fraley, Ashootosh Tripathi
  • Enzyme assay with duplicate fluorophores
    Patent number: 11685945
    Abstract: Compositions and methods are disclosed that provide a rapid, sensitive, and accurate cell-based assay for enzyme activity, particularly for enzyme activities associated with botulinum toxins. A cell is provided that expresses a construct that includes an anchor region, a cleavage site, and a reporting region having two or more identical reporter peptides. Enzymatic activity at the cleavage site releases the reporter region into the cytosol, where multiple degradation events occur. The observed change in the signal is proportional to the enzymatic activity.
    Type: Grant
    Filed: February 6, 2020
    Date of Patent: June 27, 2023
    Assignee: BioMadison, Inc.
    Inventor: Ward C Tucker
  • Detergent compositions and uses thereof
    Patent number: 11680231
    Abstract: The present invention relates to polypeptides having hexosaminidase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: June 20, 2023
    Assignee: NOVOZYMES A/S
    Inventors: Christian Berg Oehlenschlaeger, Dorotea Raventos Segura, Rebecca Munk Vejborg, Henrik Marcus Geertz-Hansen, Lilian Eva Tang Baltsen, Jesper Salomon
  • PET hydrolase having improved thermal stability
    Patent number: 11680252
    Abstract: A PET hydrolase having improved thermal stability is disclosed. The PET hydrolase has a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of asparagine at position 248 or a corresponding position with proline.
    Type: Grant
    Filed: November 22, 2021
    Date of Patent: June 20, 2023
    Assignee: HUBEI UNIVERSITY
    Inventors: Chun-Chi Chen, Jian-Wen Huang, Xiuqin Li, Wei Zeng, Lilan Zhang, Yunyun Yang, Longhai Dai, Jian Min, Yu Yang, Rey-Ting Guo
  • Recombinant acid-resistant yeast with inhibited lactate metabolism and alcohol production and method of producing lactic acid using the same
    Patent number: 11680270
    Abstract: Disclosed is a method of producing lactic acid using a recombinant acid-resistant yeast with inhibited lactate metabolism and alcohol production. More specifically, disclosed are a recombinant acid-resistant yeast in which lactate consumption reaction is reduced and which is imparted with lactic-acid-producing ability to thereby exhibit improved lactic-acid-producing ability and reduced ethanol production, and a method of producing lactic acid using the same.
    Type: Grant
    Filed: October 7, 2020
    Date of Patent: June 20, 2023
    Assignee: SK Innovation Co., Ltd.
    Inventors: Jae Yeon Park, Tae Young Lee, Ki Sung Lee
  • Method for producing objective substance
    Patent number: 11680279
    Abstract: A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism that is able to produce the objective substance, which microorganism has been modified so that the activity of an enzyme involved in SAM cycle (SAM cycle enzyme) is increased.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: June 20, 2023
    Assignee: AJINOMOTO CO., INC.
    Inventors: Benjamin Mijts, Christine Roche, Rekha Murali, Sayaka Asari, Keita Fukui, Miku Toyazaki, Keiko Noguchi
  • Cell-free protein synthesis platform derived from cellular extracts of
    Patent number: 11673921
    Abstract: Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from Vibrio species such as cellular extracts from Vibrio natriegens.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: June 13, 2023
    Assignee: Northwestern University
    Inventors: Michael Christopher Jewett, Benjamin James Des Soye, Samuel Ryan Davidson
  • Cysteine protease
    Patent number: 11667905
    Abstract: The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG.
    Type: Grant
    Filed: December 14, 2021
    Date of Patent: June 6, 2023
    Assignee: HANSA BIOPHARMA AB
    Inventors: Christian Kjellman, Sofia Jarnum, Emma Nordahl