Abstract: The invention relates to utilising red marine macroalgae to provide improvements in growth performance.

Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.

Abstract: A method and apparatus for screening of cell lines and culture conditions includes a target value setting step that sets a target value relating to a metabolic reaction of a culture cell; a first culturing step; an analysis step; an analysis value calculation step that performs a metabolism analysis based on an analysis result from the analysis step and calculates an analysis value; a culture condition computation step that computes, using a correlation model, a culture condition giving an analysis value most close to the target value, and modifies the culture condition set in the first culturing step to the computed culture condition; a second culturing step that cultures a cell under the modified culture condition; and a screening step.

Abstract: Methods and system for producing a slip stream of sugar, for example for use in the production of one or more chemicals, in ethanol fermentation facilities. In some embodiments, the methods and systems have little to no impact on the level of production of ethanol, despite also producing a slip stream of sugar. The methods and systems can be implemented in dry mill ethanol, wet mill ethanol, and lignocellulosic ethanol fermentation facilities and processes.

Abstract: The present disclosure relates to the technical field of biotechnology renewable energy, and more specifically, to a method for producing citric acid by degrading roughage with natural symbiotic mixed culture. The mixed culture YakQH5 is composed of anaerobic fungi (Neocallimastix frontalis) and methanogens (Methanobrevibacter gottschalkii). It was collected in the China General Microbiological Culture Collection Center on Mar. 9, 2020, with the collection number of CGMCC No. 19299. The mixed culture YakQH5 can degrade 15 kinds of roughage respectively and produce a large amount of citric acid. Especially when alfalfa is used as substrate, the yield of citric acid is as high as 46.0 mm. Adding compound antibiotics in the fermentation process can also prevent the mixed culture from being polluted by bacteria in the fermentation process and further improve the efficiency of anaerobic fermentation. The mixed culture YakQH5 has important industrial application value.

Abstract: A method for improving efficiency of anaerobic fermentation in a medium temperature-high temperature transition zone includes: After being subjected to heat exchange through a front heat exchanger, hydrolyzed slurry is pumped to a bottom of an anaerobic tank. A temperature of the slurry is controlled at 45±0.2° C. A central agitator is turned on after feeding is completed, to slowly gently stir the slurry in the anaerobic tank, and the central agitator is turned off after the slurry is uniformly mixed. In an operating state, making medium-temperature anaerobic flora and high-temperature anaerobic flora in methanogenic bacteria in the anaerobic sludge overlap at the fermentation temperature and be fully mixed with and in contact with the slurry, to continuously treat and convert organic substances in the slurry when the slurry is under an anaerobic fermentation condition in a medium temperature-high temperature transition zone.

Abstract: Solutions and methods are disclosed for the extraction and quantification of cellulose derived from cellulosic biomass samples. Improved extraction yields and selectivities are provided through the use of an extraction solvent mixture comprising an aprotic solvent such as DMSO, a quaternary ammonium salt such as TBAF, and an quaternary ammonium base such as TBAOH. The extracted cellulose can be optionally precipitated using disclosed precipitation solutions to further improve cellulose purity. Extracted cellulose can be measured by hydrolyzing the cellulose to glucose or cellobiose, or by using disclosed spectrophotometric assays of cellulose-salt complexes.

Abstract: Provided herein are methods involving an autocatalyzed looped relay amplification mechanism for detecting an analyte in a sample. The method is useful for detecting very low abundance analytes.

Abstract: The invention relates to the field of modified Escherichia coli strain Nissle 1917 (EcN) and its use for treating gastro-intestinal disorders. The invention is based on the study of the mechanisms implicated in the probiotic properties of the Escherichia coli strain Nissle 1917 (EcN). This study has allowed the inventors to decouple the probiotic activity of EcN from its genotoxic activity by demonstrating that EcN ClbP protein, the enzyme that activates the genotoxin colibactin, is also required for the siderophore-microcins activity of probiotic EcN, but interestingly, not its enzymatic domain that cleaves precolibactin to form active colibactin.

Abstract: It relates to Bacillus velezensis, a culture method and use thereof; Bacillus velezensis TA-3-BV serving as an endogenous antagonistic strain of sweet cherry for the first time, and a nucleotide sequence of 16S rDNA of the Bacillus velezensis TA-3-BV is shown as SEQ ID NO. 1. The Bacillus velezensis TA-3-BV has board-spectrum antibacterial activity for sweet cherry, has an inhibitory effect on a variety of pathogenic fungi, and especially has an excellent inhibitory effect on Rhizopus stolonifer serving as a soft rot pathogen of sweet cherry, with an inhibition rate of 69.82±1.43%; in addition, the Bacillus velezensis TA-3-BV has a good inhibitory effect on the postharvest rot of sweet cherry fruits, can slow down the rot process of fruits, can delay the rot of fruits by 2-3 d, thereby providing a novel method for postharvest preservation of sweet cherry.

Abstract: The invention relates to the field of microbiology and detecting micro-organism. Provided is a method for the quantitative analysis of microbial cells in a liquid sample, comprising the steps of (i) contacting the sample with a nucleic acid binding fluorescent dye to stain for nucleic acids in said microbial cells; (ii) subjecting the labelled cells to a fluorescence microscopy imaging method and determining, based on the nucleic acid staining, for a plurality of single cells at least a first parameter reflecting the nucleic acid content of said cell and a second parameter reflecting the extent of stretching of said cell; (iii) classifying based on said at least first and second parameter said cell into one of the following categories: (A) Viable cultivable cells with a nucleic acid content value between >=0.1 and <1.0 and extent of stretching of the cell value between >=1.0 and 10 ???m2, (B) Viable non-cultivable cells with a nucleic acid content value of >?0.

Abstract: The present disclosure provides a Bifidobacterium lactis (B. lactis) strain XLTG11 and a use thereof. The strain belongs to Bifidobacterium lactis. The B. lactis strain XLTG11 was deposited in the China General Microbiological Culture Collection Center (CGMCC) on Oct. 25, 2019, with a taxonomic name of Bifidobacterium lactis XLTG11 and an accession number of CGMCC No. 18738. The B. lactis strain XLTG11 provided by the present disclosure can regulate an inflammatory cytokine, improve an intestinal barrier function, and modulate an intestinal flora by inhibiting the activation of a TLR4/MYD88/NF-?B signaling pathway, thereby alleviating dextran sulfate sodium (DSS)-induced colitis. In addition, the B. lactis strain XLTG11 has an immune-enhancing effect on a cyclophosphamide (CTX)-induced mouse immunosuppression model.

Abstract: Compositions comprising extracellular matrix (ECM) materials and methods of use thereof are disclosed. The compositions may comprise two or more ECM materials derived from different types of tissues, such as, e.g., lung tissue and spleen tissue, formulated for administration to a patient or configured as a medical device for implantation in or application to the patient. The compositions may combine complementary properties of different types of ECM materials for customized patient-specific and/or site-specific tissue repair and/or regeneration.

Abstract: The present invention discloses the use of plasminogen to regulate GLP-1/GLP-1R and treat a GLP-1/GLP-1R-related condition.

Abstract: The presently disclosed subject matter relates to isolated compositions and methods for treating and/or preventing sepsis and inflammatory conditions, such as Acute Respiratory Distress Syndrome (ARDS). In some embodiments, the presently disclosed subject matter relates to endothelial progenitor cell-derived exosomes to treat and/or prevent sepsis and inflammatory conditions, such as Acute Respiratory Distress Syndrome (ARDS). The endothelial progenitor cell-derived exosomes can be modified to overexpress one or more miRNAs.

Abstract: Provided are a cell population substantially consisting of mammalian cells that are positive for cell surface markers CD31 and CD200 and negative for CD45; a cell population substantially consisting of mammalian cells that are positive for cell surface markers CD31, CD157 and CD200 and negative for CD45; a mammalian vascular endothelial stem cell that is positive for the cell surface marker CD31, positive for at least one of CD157 and CD200, and negative for CD45; and a medicament for vascular regeneration, comprising any of the cell populations or the vascular endothelial stem cells as an active ingredient.

Abstract: Compositions comprising a population of oligodendrocyte progenitor cells (OPCs), as well as methods of making and using the same, are provided. In one aspect, a container comprising a composition, where the composition comprises a population of cells comprising a plurality of OPCs, and where the population of cells comprises less than 15% undesirable cell types is provided. In another aspect, the population of cells comprises less than 15% undesirable epithelial lineage cells. In yet another aspect, the population of cells comprises less than 2% K7 positive cells. In an aspect, a population of cells comprising a plurality of oligodendrocyte progenitor cells is capable of forming less than one epithelial cyst per 100,000 cells in a cyst assay is provided. An even further aspect of the present disclosure is a container comprising a composition, where the composition comprising a plurality of oligodendrocyte progenitor cells is useful in treating treat stroke, spinal cord injury, and multiple sclerosis.

Abstract: The present invention relates to a method of reducing total gas production and/or methane production in a ruminant animal.

Abstract: The present invention provides a novel daptomycin-producing Streptomyces strain and use thereof, the strain is a subspecies of streptomyces griseus, named Streptomyces griseus L340, and the preservation number is CGMCC17921. The genetic character and fermentation unit of the strain provided by the present invention are relatively stable, the culture and fermentation conditions are suitable for industrial production of daptomycin, and the interference of pigment in later separation and purification is eliminated. The secondary metabolite daptomycin is obtained through fermentation of the Streptomyces provided by the present invention, which has the advantages of no interference of pigment accumulation, cleaner metabolic spectrum and thus is obviously superior in later separation and purification.

Abstract: Methods for improving quality of life and/or increasing activity in an aging and/or chronically ill mammal via administration of a composition of egg powder protein are provided.