Abstract: The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment.The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules.The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

Abstract: The present invention provides methods of Agrobacterium-mediated transformation of monocots. The invention further provides methods of making transgenic monocots, as well as transgenic monocots made by the present methods, and seeds and progeny thereof.

Abstract: A method of making recombinant plant cells having reduced variability of expression of foreign genes therein is described herein. The method comprises (a) providing a plant cell capable of regeneration; and (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and an insulator (or "boundary element") positioned (i) 5' to the transcription initiation region, (ii) 3' to the structural gene, or (iii) both 5' to the transcription initiation region and 3' to the structural gene. DNA constructs useful for carrying out the method and plant cells and plants produced by the method are also disclosed.

Abstract: A marker gene for use in the genetic transformation of plants is driven by a promoter which is capable of acting as a callus-specific promoter, for example in monocotyledonous plants. A suitable promoter naturally drives the expression of a gene encoding a predicted 16.92 kDa protein after wounding and/or callus formation in Asparagus officinalis or an equivalent protein in other members of Liliaceae or Amaryllidaceae. A preferred embodiment is designated the AoPR1 promoter. Under the control of a promoter of the invention, the marker gene (which may for example be an antibiotic- or herbicide-resistance gene) is expressed strongly in wounded tissues and cells and cultured explants, but not constitutively throughout the whole plant. Such promoters may also be used to drive expression of genes in plant tissue culture.

Abstract: Four novel Bacillus thuringiensis strains, which are deposited at the BCCM-LMG under accession nos. LMG P-12592, LMG P-12593, LMG P-12594, and LMG P-13493, produce new crystal proteins during sporulation that are toxic to Lepidoptera, more particularly against Noctuidae such as spodoptera spp. and Agotis ipsilon, against Pyralidae such as Ostrinia nubilalis, and against Yponomeutidae such as Plutella xylostella, and that are encoded by a novel gene. The crystal proteins contain protoxins, which can yield a toxin as trypsin-digestion product. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains nd that encodes its respective toxin, is resistant to Lipidoptera. Each strain, itself, or its crystal, crystal protein, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combating Lepidoptera.

Abstract: The present invention is directed to isolated non-coding nucleotide sequences useful as promoters for heterologous gene expression in plants. The present invention is also directed to vectors and plant cells comprising the isolated nucleotide sequences.AHAS promoters from maize are used to express introduced genes at high levels and in various plant tissues. Promoters from the als1 and als2 genes of maize are cloned and sequenced, and the promoter regions from these genes are then introduced into a plasmid 5' to the reporter gene beta-glucuronidase (GUS). Both promoter fragments are from the XI12 maize line. The als1 promoter fragment is approximately 1400 base pairs long, whereas the als2 promoter fragment contains 829 base pairs.

Abstract: Disclosed are methods and compositions for increasing the expression and recovery of heterologous polypeptides secreted by cultured plant cells, including DNA constructs for high-level gene expression, seretion of heterologous polypeptides through both the plasma membrane and cell wall into the plant culture medium to facilitate protein recovery, and novel plant culture media. Recovery of secreted heterologous polypeptides from plant cell culture medium is significantly improved by including in the plant culture medium a polypeptide stabilizer such as polyvinylpyrrolidone.

Abstract: A method of inducibly enhancing the constitutive expression of a DNA sequence of interest is described in which plant cells are transformed with a DNA sequence of interest that is operably joined to a plant ubiquitin regulatory region comprised of a heat shock element, a promoter, a transcription start site, an intron and a translation start site, When monocot or dicot plant cells are subjected to permissive heat shock temperatures, the level of expression of the DNA sequence of interest is enhanced.

Abstract: A full-length transcript promoter from figwort mosaic virus (FMV) is identified and its DNA sequence given. The promoter functions as a strong and uniform promoter for chimeric genes inserted into plant cells. This strong promoter function is exhibited by a histochemical assay in floral buds and by reproductive scores of transgenic plants including the promoter. The promoter preferably includes a 5' leader sequence that may be from the FMV itself or from a heterologous source with respect to the promoter. The promoter is used in a plant cassette vector, a chimeric gene and in methods for transforming plant cells to obtain transgenic plants, plant cells or seeds incorporating the FMV promoter.

Abstract: DNA sequences comprising, as coding region, all or part of the nucleotide sequence coding for an mRNA coding for a cinnamoyl CoA reductase (CCR), or all or part of the complementary nucleotide sequence thereof and coding for an anti-sense mRNA capable of hybridizing with the above-mentioned mRNA. The invention also concerns the use of said sequences for carrying out methods of regulation of plant lignin biosynthesis.

Abstract: This invention provides novel gene constructs which enhance the efficiency of plant cells and cells of other photosynthetic organisms. Also provided are transgenic plants and seeds which overexpress proteins. Methods to elevate the amount of plastid proteins in plants and photosynthetic organisms are exemplified.

Abstract: Nematode-resistant transgenic plants are disclosed. The plants comprise plant cells containing a DNA construct comprising a transcription cassette, which construct comprises, in the 5' to 3' direction, a promoter operable in the plant cells, and a DNA comprising at least a portion of a DNA sequence encoding a nematode-inducible transmembrane pore protein in either the sense or antisense orientation. Intermediates for producing the same along with methods of making and using the same are also disclosed. In an alternate embodiment of the invention, the sense or antisense DNA is replaced with a DNA encoding an enzymatic RNA molecule directed against the mRNA transcript of a DNA sequence encoding a nematode-inducible transmembrane pore protein.

Abstract: The present invention provides a rapid and efficient method for transforming monocotyledonous plants. The invention particularly relates to the use of either intact tissue capable of forming compact embryogenic callus or compact embryogenic callus obtained from such tissue to obtain transgenic monocotyledonous plants. The present invention also provides novel transgenic plants obtained by the transformation method of the present invention.

Abstract: The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide in Erwinia chrysanthemi which elicits a hypersensitive response in plants. The encoding DNA molecule alone in isolated form or either in an expression system, a host cell, or a transgenic plant are also disclosed. Another aspect of the present invention relates to a method of imparting pathogen resistance to plants by transforming a plant with the DNA molecule of the present invention.

Abstract: According to the invention, there is provided an inbred corn plant designated NL085B. This invention thus relates to the plants, seeds and tissue cultures of the inbred corn plant NL085B, and to methods for producing a corn plant produced by crossing the inbred plant NL085B with itself or with another corn plant, such as another inbred. This invention further relates to corn seeds and plants produced by crossing the inbred plant NL085B with another corn plant, such as another inbred, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of the inbred corn plant NL085B, and also to the RFLP and genetic isozyme typing profiles of inbred corn plant NL085B.

Abstract: This invention provides for the secretion of heterologous protein in plant systems. In particular, this invention provides for the production of heterologous proteins by malting of monocot plant seeds. The heterologous genes are expressed during germination of the seeds and isolated from a malt. Also disclosed are chimeric genes, vectors and methods relating to the present invention. Protein production by cell culture techniques is also described.

Abstract: The present invention relates generally to a nucleic acid molecule encoding, or complementary to a nucleic acid molecule encoding, a polypeptide which confers, enhances, or otherwise facilitates resistance to a nematode in a plant cell. The nucleic acid molecule of the present invention is useful in the isolation of related nematode resistance, or nematode resistance-like genetic sequences, from other plants. Furthermore, the present invention provides for the generation of plants carrying non-endogenous nematode resistance, or nematode resistance-like genetic sequences, said plants exhibiting enhanced tolerance to parasitic nematodes and related pathogens.

Abstract: An inbred corn line, designated LH261, is disclosed. The invention relates to the seeds of inbred corn line LH261, to the plants of inbred corn line LH261 and to methods for producing a corn plant produced by crossing the inbred line LH261 with itself or another corn line. The invention further relates to hybrid corn seeds and plants produced by crossing the inbred line LH261 with another corn line.

Abstract: A method is disclosed for making more efficient the particle-mediated germ line genetic transformation of bean species such as soybean. After a particle-mediated transformation event, in the absence of a selectable marker gene, relatively large numbers of plants must be regenerated to find the relatively low likelihood germ line transformation events which have occurred. It has been discovered that using in the transformation process a marker gene linked to the gene of interest, and by excising a segment of the stem of the shoot during the regeneration process and assaying the segment for the marker gene, certain patterns or phenotypes can be identified in the stem segment which are associated with an increased frequency of germ line transformation events. As the plants are regenerated, other indices of gene expression, at the first trifoliate leaf stage and at the third or fourth trifoliate leaf stage, also serve as markers of the likelihood of germ line transformation.

Abstract: An inbred sweet corn line, designated W1498A, the plants and seeds of inbred sweet corn line W1498A, methods for producing a maize plant produced by crossing the inbred sweet corn line W1498A with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred sweet corn line W1498A with another maize line or plant.