Abstract: Described are an isolated DNA fragment incorporating an osmotin gene promoter sequence, recombinant DNA incorporating a foreign structural gene under control of an osmotin gene promoter sequence, as well as methods and transformants involving the isolated DNA fragment and recombinant DNA. Also described are methods for the inhibition of fungal, insect, nematode, and viral pathogens in a plant using such recombinant DNA.
Abstract: A method for obtaining plants with reduced susceptibility to invasion by a plant parasitic nematode, and plants having reduced susceptibility to invasion by a plant parasitic nematode obtained, for example, with such method.
Type:
Grant
Filed:
May 19, 1994
Date of Patent:
February 2, 1999
Assignee:
Mogen International, N.V.
Inventors:
Peter Christiaan Sijmons, Oscar Johannes Maria Goddijn, Petrus Josephus Maria Van Den Elzen, Frederique Marianne Van Der Lee
Abstract: A method for producing a genetically modified plant by introducing a polynucleotide to an intact plant or plant cell(s) via electroporation, in the absence of cell wall-degrading enzymes. Genetically engineered plants produced by the method of the invention are also provided. The invention also provides a method for producing a polypeptide in an intact plant cell, including plant tissue or a whole plant by introducing a biologically active polypeptide directly into the plant.
Abstract: Sugar beet plants which are resistant to both imidazolinone and sulfonylurea herbicides are described. The sugar beet plants are derived from susceptible cells by sequential selection of SU-R cells, plant regeneration, and re-selection for IM-R. The resistant sugar beet plants derived from the cells can be grown in fields where imidazolinones and sulfonylureas have been used for weed control.
Type:
Grant
Filed:
December 6, 1996
Date of Patent:
January 12, 1999
Assignee:
Board of Trustees Operating Michigan State University
Abstract: A modular promoter construct is described which possesses a promoter which is active in plant cells and a DNA sequence from exon 1 of the rice actin 1 gene. This modular promoter construct gives rise, where appropriate together with additional regulatory DNA sequences, to a substantial increase in gene expression in plant cells.
Type:
Grant
Filed:
February 7, 1995
Date of Patent:
January 12, 1999
Assignee:
Maxplanck-Gesellschaft Zur Forderung Der Wissenschaften E.V.
Inventors:
Christoph Maas, Jeff Schell, Hans-Henning Steinbiss
Abstract: Isolated DNA elements that direct either pistil-specific or anther-specific expression of a polypeptide-encoding gene are disclosed. Plants comprising the DNA fragment operably linked to a gene are also disclosed. A further embodiment comprises a method of producing a female sterile plant, the method comprising growing a plant having integrated into its genome an isolated DNA element that directs pistil-specific expression of a nucleotide sequence that encodes or is transcribed into a moiety that causes female sterility in plants.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
January 12, 1999
Assignee:
Cornell Research Foundation, Inc.
Inventors:
Mikhail E. Nasrallah, June B. Nasrallah, Mary K. Thorsness
Abstract: The present invention relates to an isolated protein or polypeptide corresponding to a protein or polypeptide in Erwinia chrysanthemi which elicits a hypersensitive response in plants. The encoding DNA molecule alone in isolated form or either in an expression system, a host cell, or a transgenic plant are also disclosed. Another aspect of the present invention relates to a method of imparting pathogen resistance to plants by transforming a plant with the DNA molecule of the present invention.
Abstract: A 1.2 kb fragment of the 5' regulatory region (from bp -1107 to +100) was fused to the GUS (.beta.-glucuronidase) reporter gene and BN115-promoted GUS expression was observed in green tissues of transgenic Brassica napus plants only after incubation at 2.degree. C. No expression was observed after incubation at 22.degree. C., either in the presence or absence of abscisic acid. Microprojectile bombardment of winter B. napus leaves with a BN115 promoter/GUS construct yielded similar results and was used to analyze a series of deletions from the 5' end of the promoter. Results obtained from transient expression studies showed that the low temperature regulation of BN115 expression involves a possible enhancer region between bp -1107 and -606 and a second positive regulatory region located between bp -302 and -274.
Type:
Grant
Filed:
April 12, 1995
Date of Patent:
December 8, 1998
Assignees:
Her Majesty the Queen in right of Canada as represented by the Minister of Agriculture, Agri-Food Canada
Inventors:
Jas Singh, Theresa Catherine White, Chao Jiang
Abstract: A method of inhibiting the production of mycotoxins of fungus, such as aflatoxin-producing and sterigmatocystin-producing fungi, in plants susceptible to contamination by such mycotoxins consists of introducing into the susceptible plant a gene encoding for lipoxygenase pathway enzyme of the mycotoxin. Exemplary of the lipoxygenase pathway enzymes are soybean lipoxygenase, allene oxidase, hydroperoxide lyase and hydroperoxide dehydratase. The resulting transgenic plant demonstrates substantial resistance to mycotoxin contamination of such fungus. Plants which are substantially resistant to mycotoxin contamination of Aspergillus spp. are further obtained by incorporating into mycotoxin susceptible plant antisense genes for the 9-hydroperoxide fatty acid producing lipoxygenases.
Abstract: Methods and compositions based upon natural aromatic compounds are provided, which find use as pesticides. The pesticides are formulated in a variety of ways, including dusts, sprays, shampoos and soaps, and can be bound to a solid support or provided as bait or directly impregnated into organic matter infested by or susceptible to infestation by a target pest. Pests controlled include mosquitos, lice, ants, cockroaches, lice, and ticks.
Type:
Grant
Filed:
March 25, 1996
Date of Patent:
November 24, 1998
Assignee:
Proguard, Inc.
Inventors:
Ralph W. Emerson, Bradford G. Crandall, Jr.
Abstract: A method for producing transgenic seeds and plants, which comprises isolating, from anthers, microspores in their uninucleate stage and removing tissue in which they are embedded, culturing the isolated microspores in a nutrient solution, transferring foreign genetic material into the isolated microspores to obtain transformed microspores, bringing about complete maturation of the transformed microspores to obtain transformed pollen grains in vitro, pollinating receiver plants with the transformed pollen grains and obtaining seeds from the pollinated receiver plants.
Type:
Grant
Filed:
March 27, 1997
Date of Patent:
November 24, 1998
Assignee:
Mogen International nv
Inventors:
Erwin Heberle-Bors, Rosa Maria Benito Moreno, Anna Alwen, Alisher Tourajew, Eva Maria Stoger
Abstract: An isolated DNA molecule which comprises a DNA promoter sequence which directs root cortex specific transcription of a downstream heterologous DNA segment in a plant cell. A DNA construct which comprises an expression cassette comprising, in the 5' to 3' direction, a promoter of the present invention and a heterologous DNA segment positioned downstream from the promoter and operatively associated therewith. Transformed plants, such as tobacco plants, comprise transformed plant cells containing a heterologous DNA construct comprising an expression cassette as described above.
Type:
Grant
Filed:
July 28, 1995
Date of Patent:
November 17, 1998
Assignee:
North Carolina State University
Inventors:
Mark A. Conkling, Nandini Mendu, Wen Song
Abstract: A DNA which has the sequence shown in FIG. 5 and which defines a gene promoter region has been isolated from maize roots. The promoter may be used for driving expression of foreign genes in the roots of plants. This is particularly useful for expressing an insecticidal toxin, such as a delta-endotoxin of Bacillus thuringiensis, to impart resistance to insect attack on the roots of plants by Coleopteran insects.
Type:
Grant
Filed:
August 10, 1994
Date of Patent:
November 17, 1998
Assignee:
Zeneca Limited
Inventors:
Susan Ely, Ian Jeffrey Evans, Wolfgang Walter Schuch
Abstract: A method is disclosed for making more efficient the particle-mediated germ line genetic transformation of bean species such as soybean. After a particle-mediated transformation event, in the absence of a selectable marker gene, relatively large numbers of plants must be regenerated to find the relatively low likelihood germ line transformation events which have occurred. It has been discovered that using in the transformation process a marker gene linked to the gene of interest, and by excising a segment of the stem of the shoot during the regeneration process and assaying the segment for the marker gene, certain patterns or phenotypes can be identified in the stem segment which are associated with an increased frequency of germ line transformation events. As the plants are regenerated, other indices of gene expression, at the first trifoliate leaf stage and at the third or fourth trifoliate leaf stage, also serve as markers of the likelihood of germ line transformation.
Abstract: Nucleotide sequences encoding a maize 5C9 polypeptide, the 5C9 polypeptide and structural and functional equivalents, and methods for controlling insect infestation in plants are provided.
Type:
Grant
Filed:
May 25, 1995
Date of Patent:
October 20, 1998
Assignee:
Pioneer Hi-Bred International, Inc.
Inventors:
Timothy W. Fox, Carl W. Garnaat, Terry EuClaire Meyer
Abstract: A method for commercial production of aprotinin entails heterologous expression of the protein in plants, preferably at a level such that aprotinin accounts for at least 0.1% of the total extracted protein. An aprotinin-expressing plant also has potential of increased insecticidal resistance by virtue of producing the protein. A genetic map of the integration locus allows identification of plants derived from the transgenic plant. This approach also reveals genetic loci on a plant chromosome that support high levels of gene expression and can be used as site of integration for expression of other genes of interest.
Type:
Grant
Filed:
November 6, 1995
Date of Patent:
October 20, 1998
Inventors:
Chris Baszczynski, Thomas Czapla, Elizabeth Hood, Terry EuClaire Meyer, David Peterson, A. Gururaj Rao, James C. Register, III, Derrick Witcher, John A. Howard
Abstract: A method of controlling nematodes, the method includes the steps of identification of a gene induced within a successfully infected plant by nematode infection of said plant and modifying the gene to confer nematode resistance to the plant.
Abstract: Methods of providing and breeding trees having more easily extractable lignin due to the presence of a cinnamyl alcohol dehydrogenase (Cad) null gene are presented.
Type:
Grant
Filed:
July 26, 1996
Date of Patent:
October 20, 1998
Assignee:
North Carolina State University
Inventors:
John MacKay, David O'Malley, Ross Whetten, Ronald Sederoff
Abstract: By selection for resistance to aryloxyphenoxyalkane-carboxylic acid herbicides, herbicide-tolerant maize cell lines, calli and plants regenerated therefrom can be obtained which pass this herbicide tolerance on to their progeny in a stable manner.
Abstract: A method for commercial production of GUS entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the GUS-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of GUS expression and that can be used as a site of integration for high level expression of other genes of interest.
Type:
Grant
Filed:
November 6, 1995
Date of Patent:
September 8, 1998
Assignee:
ProdiGene, Inc.
Inventors:
Wesley B. Bruce, Elizabeth Hood, David J. Peterson, James C. Register, III, Derrick Witcher, John A. Howard