Patents Examined by Tracy Vivlemore
  • Patent number: 10415034
    Abstract: Disclosed is a method for the selection of aptamers that does not require immobilization of the target or the oligonucleotide library. The method includes: (i) combining a selection library of oligonucleotides that contain a random region flanked by primer recognition sites with blocker oligonucleotides that anneal to the primer recognition sites; (ii) exposing the blocked selection library to an immobilization field including random oligonucleotides and removing unbound selection library oligonucleotides; (iii) recovering the selection library oligonucleotides that bound to the immobilization field and combining with a target analyte of interest; (iv) exposing the selection library oligonucleotides-target analyte mixture to an immobilization field; and (v) recovering those selection library oligonucleotides that did not bind to the immobilization field.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: September 17, 2019
    Inventor: Gregory Penner
  • Patent number: 10415033
    Abstract: Disclosed herein is a phage-displayed single-chain variable fragment (scFv) library, which comprises a plurality of phage-displayed scFvs characterized in having a specific CS combination and a specific sequence in each CDR. The present scFv library is useful in efficiently producing different antibodies with binding affinity to different antigens. Accordingly, the present disclosure provides a potential means to generate different antigen-specific antibodies promptly in accordance with the need in experimental researches and/or clinical applications.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: September 17, 2019
    Assignee: Academia Sinica
    Inventors: An-Suei Yang, Jhih-Wei Jian, Hong-Sen Chen, Yi-Kai Chiu, Hung-Pin Peng, Chao-Ping Tung, Chung-Ming Yu, Wei-Ying Kuo, Hung-Ju Hsu
  • Patent number: 10415032
    Abstract: A method for determining past exposure to chemical agents or heavy metals may include coating a capture material with a capture reagent. The capture reagent may be selected based on an ability of the capture reagent to bind with a target antibody, and the target antibody may be an indicator associated with a particular chemical agent or heavy metal. The method may further include interrogating a clinical sample associated with an individual by forming a mixture of the capture material and the clinical sample, and determining an exposure status of the individual to the particular chemical agent or heavy metal based on whether the capture material demonstrates capture of the indicator.
    Type: Grant
    Filed: August 29, 2016
    Date of Patent: September 17, 2019
    Assignee: The Johns Hopkins University
    Inventor: Mekbib Astatke
  • Patent number: 10407678
    Abstract: Disclosed herein are compositions and methods for modulating expression of C9ORF72 antisense transcript in a cell or animal with C9ORF72 antisense transcript specific inhibitors. Such methods are useful to treat, prevent, or ameliorate neurodegenerative diseases in an individual in need thereof. Such C9ORF72 antisense transcript specific inhibitors include antisense compounds.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: September 10, 2019
    Assignee: Ionis Pharmaceuticals, Inc.
    Inventor: Frank Rigo
  • Patent number: 10400238
    Abstract: The invention relates to polynucleotide agents targeting the complement component C5 gene, and methods of using such polynucleotide agents to inhibit expression of C5 and to treat subjects having a complement component C5-associated disease, e.g., paroxysmal nocturnal hemoglobinuria.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: September 3, 2019
    Assignee: Alnylam Pharmaceuticals, Inc.
    Inventor: Gregory Hinkle
  • Patent number: 10400234
    Abstract: A library of replicating entities, each entity comprises a recombinant vector comprising a randomized nucleic acid sequence, having the reading frame structure [NXX]n [CorAA] [NXX]m [NZZ]o, or [NZZ]o [NXX]m [CorAA] [NXX]n. Each NXX is independently a codon encoding for any amino acid except cysteine, CorAA is a codon encoding for cysteine or at least one other amino acid, each NZZ is independently a codon encoding for any amino acid, and n is an integer from 0 to 40, m is an integer from 1 to 20, o is an integer from 1 to 40, and at least 20 percent of CorAA encode for cysteine. The invention further relates to a set of recombinant vectors and to a set of randomized oligonucleotides, each oligonucleotide having said structure, as well as to a method for generating a library of replicating entities and to a method for identifying an amino acid polymer.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: September 3, 2019
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
    Inventors: Michael Szardenings, Nicolas Delaroque
  • Patent number: 10398722
    Abstract: The present invention concerns a treatment of diseases involving B cells, B lymphocytes or plasmocytes. In particular, it concerns an antisense oligonucleotide or a mixture of antisense oligonucleotides capable of inducing exon skipping at the RNA of the immunoglobulin heavy or light chains, for the use of same in the treatment of diseases involving B cells.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: September 3, 2019
    Assignees: Universite de Limoges, Centre National de la Recherche Scientifique
    Inventors: Laurent Delpy, Nivine Srour, Michel Cogné
  • Patent number: 10398721
    Abstract: Provided are methods of treatment in subjects having progeroid diseases and related conditions which rely upon LMNA-targeted antisense oligonucleotides for reducing expression of one or more aberrantly spliced LMNA mRNA isoforms that encode progerin.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: September 3, 2019
    Inventors: Ryszard Kole, Francis S. Collins, Michael R. Erdos, Kan Cao, Leslie B. Gordon
  • Patent number: 10385475
    Abstract: The invention is directed to a method of sequencing low-complexity amplicons randomly arrayed at high density on a surface. Methods of the invention include preparing amplicons for sequencing by a sets of primers that ensure initial signals front different amplicons on the surface will be evenly distributed among the different nucleotides being added in a sequencing by synthesis operation.
    Type: Grant
    Filed: August 28, 2012
    Date of Patent: August 20, 2019
    Inventors: Jianbiao Zheng, Thomas Willis, Malek Faham
  • Patent number: 10385391
    Abstract: Methods and compositions are provided for performing a set of N DNA sequencing reaction cycles whereby sequence information is obtained for approximately 2*N nucleotide bases.
    Type: Grant
    Filed: September 22, 2010
    Date of Patent: August 20, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Frederick P. Roth, Joseph C. Mellor, Yong Lu, Mark Chee
  • Patent number: 10386338
    Abstract: The present invention relates to a piezoelectric mechanical system (PEMS) microcantilever sensor that both detects the presence of viral RNA in an aqueous solution, such as a blood sample. The method provides for the formation of the sensor by attaching RNA, DNA, or an antibody to the microcantilever sensor surface via a hydrazone or an oxime chemical bond. The method provides for the detection of viral RNA viruses and viral DNA viruses upon the chemical binding/bonding of single-stranded viral nucleic acid to the microcantilever sensor surface. The method provides for the detection of DNA cancer mutations or variants that have been identified in a cancer cell upon the chemical binding/bonding of single-stranded DNA to the microcantilever sensor surface.
    Type: Grant
    Filed: June 21, 2018
    Date of Patent: August 20, 2019
    Inventor: Cynthia Rena Wright
  • Patent number: 10384187
    Abstract: Complete nucleic acid library preparation devices are provided. Aspects of the devices include: a thermal chip module comprising multiple nodes; one or more plate locations; a robotically controlled liquid handler configured to transfer liquid between the one or more plate locations and the thermal chip module; and a bulk reagent dispenser configured to access each node of the thermal chip module.
    Type: Grant
    Filed: February 9, 2015
    Date of Patent: August 20, 2019
    Inventors: Kieran Curran, Brian Chawke, Noel Sirr
  • Patent number: 10378054
    Abstract: There is provided a sol-gel chip using a porous substrate for entrapping small molecules and a method for screening a small molecule-specific material using the same, and more particularly, a porous substrate sol-gel chip characterized in that a sol-gel composition for entrapping small molecules is spotted on a surface of the porous substrate, a method for manufacturing the porous substrate sol-gel chip for entrapping small molecules, and a method for screening a material specifically binding to the small molecules using the porous substrate sol-gel chip for entrapping small molecules. According to the present invention, the small molecules can be effectively entrapped in the chip and the inflow of aptamers can be maintained as compared with the existing methods and thus aptamers specific to an extensive range of small molecules can be more easily selected.
    Type: Grant
    Filed: January 26, 2013
    Date of Patent: August 13, 2019
    Inventors: So Youn Kim, Ji Young Ahn
  • Patent number: 10378051
    Abstract: A reaction mixture including (a) a nucleic acid having a primer hybridized to a template, (b) nucleotide analogs, wherein the nucleotide analogs have a blocking moiety; (c) a polymerase that is capable of forming an extended primer by adding the nucleotide analogs to the primer, and (d) a deblocking agent that is capable of removing the blocking moiety from the extended primer. Also provided is a method of synthesizing a polynucleotide including sequentially adding a plurality of the different nucleotides analogs to the nucleic acid via several reaction cycles in the reaction mixture, wherein each reaction cycle includes (i) the polymerase adding a nucleotide analog to the nucleic acid to form a transient nucleic acid species comprising a blocking moiety, and (ii) the deblocking agent modifying the transient nucleic acid species to remove the blocking moiety.
    Type: Grant
    Filed: September 26, 2012
    Date of Patent: August 13, 2019
    Assignee: Illumina Cambridge Limited
    Inventors: Wouter Meuleman, Elena Cressina, Geoffrey Paul Smith, Rosamond Jackson, Xiaohai Liu
  • Patent number: 10378016
    Abstract: The invention provides aptamer-gene modulator conjugates, where the aptamer and the gene modulator are linked together. The invention further provides a method for cell-specific delivery of gene modulators to hard to transfect cells such as CD4+ cell.
    Type: Grant
    Filed: December 6, 2011
    Date of Patent: August 13, 2019
    Assignee: Children's Medical Center Corporation
    Inventors: Judy Lieberman, Lee Adam Wheeler
  • Patent number: 10364440
    Abstract: The present invention is directed to transfection complexes of rosette nanotubes and one or more nucleic acids.
    Type: Grant
    Filed: January 3, 2012
    Date of Patent: July 30, 2019
    Assignees: Brown University, Rhode Island Hospital, The Governors of the University of Alberta, National Research Council of Canada
    Inventors: Thomas J. Webster, Qian Chen, Yupeng Chen, Hicham Fenniri, Usha Devi Hemraz
  • Patent number: 10358641
    Abstract: Provided herein are monoliths with attached recognition compounds which selectively bind ligands, methods of preparing such monoliths, arrays thereof and uses thereof. For example, monoliths provide herein can be used in columns and arrays thereof.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: July 23, 2019
    Assignee: DiCE Molecules SV, LLC
    Inventors: Pehr Harbury, Madan Paidhungat, Robin Prince
  • Patent number: 10357543
    Abstract: Disclosed are methods and compositions related to treatment and prevention of sarcopenia and/or nerve injury by increasing survival motor neuron (SMN) levels in an individual in need thereof.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: July 23, 2019
    Assignees: Ohio State Innovation Foundation, The Research Institute At Nationwide Children's Hospital
    Inventors: Arthur Burghes, William Arnold, Brian Kaspar, Vicki McGovern
  • Patent number: 10352927
    Abstract: It is intended to develop and provide a method for detecting a particular glycan-isoform rapidly and specifically by a small number of steps. The present invention provides a glycan-isoform detection method comprising quantifying an immune complex formed by the mixing of a test sample with a sugar chain non-reducing terminal residue-binding lectin and an antibody specifically binding to the protein moiety of the glycan-isoform, etc., comparing the obtained amount of the immune complex with the amount of a control immune complex obtained when a control sample is not mixed with the sugar chain non-reducing terminal residue-binding lectin or is mixed with a control protein, and determining the presence or absence of the glycan-isoform of interest in the test sample on the basis of the difference between these amounts.
    Type: Grant
    Filed: August 9, 2013
    Date of Patent: July 16, 2019
    Inventors: Hisashi Narimatsu, Atsushi Kuno, Yuzuru Ikehara, Yasuhiro Hashimoto, Keiro Shirotani, Kiyomitsu Nara, Yoshinobu Kariya, Hiromi Ito, Kyoka Hoshi
  • Patent number: 10351846
    Abstract: An apparatus and method for applying a chemistry to samples of interest are provided. The apparatus and method include a flexible tape mounted on an arrangement of guide rollers. Samples of interest (e.g., clusters of DNA templates) are bound to at least one surface of the flexible tape. The method and apparatus further comprise one or more read heads in relation to the flexible tape and a plurality of reservoirs along a path of the flexible tape. The reservoirs comprise liquids comprising chemical reagents for performing the chemistry on the samples of interest bound to the at least one surface of the flexible tape. The method and apparatus further comprise a drive system for driving at least one of the guide rollers to advance the flexible tape into and out of the reservoirs.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: July 16, 2019
    Inventors: Jonathan Mark Boutell, Gary Mark Skinner, Gareth Jenkins