Abstract: Methods for providing compositions to corn fields prior to harvesting are provided herein. These methods provide an extended time period for the use of lower height or standard height farm equipment in-season in corn fields, while reducing the risk of damage to the corn plants. These methods also allow for late season access with lower height or standard height farm equipment, while reducing the risk of damage to the corn plants.

Abstract: A donor construct and a gene knockout method, as well as a system and kit for the gene knockout are provided. The donor construct is a linear donor DNA or can be cleaved in a cell to produce the linear donor DNA. The gene knockout method uses a marker gene contained in the donor construct to enrich cells in which a gene is knocked out, thereby improving the efficiency of generating the gene knockout by a sequence-specific nuclease.

Abstract: A soybean cultivar designated 03070412 is disclosed. The invention relates to the seeds of soybean cultivar 03070412, to the plants of soybean cultivar 03070412, to the plant parts of soybean cultivar 03070412, and to methods for producing progeny of soybean cultivar 03070412. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 03070412. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 03070412, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 03070412 with another soybean cultivar.

Abstract: The invention relates to methods for improving abiotic stress tolerance (and/or yield) in plants, including enhanced drought tolerance. It discloses nucleic acid constructs comprising the isolated polynucleotides, transgenic plants expressing the same, and methods of using the same for increasing abiotic stress tolerance (and/or yield).

Abstract: A soybean cultivar designated 06310200 is disclosed. The invention relates to the seeds of soybean cultivar 06310200, to the plants of soybean cultivar 06310200, to the plant parts of soybean cultivar 06310200, and to methods for producing progeny of soybean cultivar 06310200. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar 06310200. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar 06310200, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar 06310200 with another soybean cultivar.

Abstract: The present disclosure relates to a mutant cell-free DNA isolation kit and a mutant cell-free DNA analysis method using a CRISPR-Cas system and an exonuclease.

Abstract: The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

Abstract: The invention relates to the production of graft-mediated hybrid monocotyledonous plants. Methods for the production of such plants are disclosed herein.

Abstract: A novel maize variety designated X03P560 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X03P560 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X03P560 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X03P560, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X03P560 are provided. Methods for producing maize varieties derived from maize variety X03P560 and methods of using maize variety X03P560 are disclosed.

Abstract: The invention relates to binary vectors based on compatible and autonomous origins, specifically based on the pBBR1 and RK2 replication origins. These binary vectors are useful for having a wide range of hosts, for their maintenance in Agrobacterium sp. and Escherichia coli, and as a new tool for plant synthetic biology as well as a flexible framework for assembly, transfer and characterization of multiple DNA elements. The binary vectors disclosed are small, preferably less than 3.8 kb in size, stable, include an origin compatible with the most commonly used binary T-DNA vectors, comply with current standards for plant synthetic biology, and allow the administration of multiple T-DNA cassettes by means of the multiplexing of the vectors. The present invention also relates to methods for transferring and expressing nucleic acid sequences using said binary vectors, and to the uses of the same.

Abstract: Autotrophic nitrogen fixation genes and an autotrophic nitrogenase expression cassette for plants and uses thereof are disclosed. Nitrogenase related genes of nitrogen fixation gene cluster from Paenibacillus sp. are specifically optimized by plant expression patterns through synthetic biology, to obtain autotrophic nitrogen fixation genes, which are then constructed into plant constitutive expression units. The expression units are assembled in a plant expression vector by isocaudarner cloning method and transformed into plants, thereby realizing autotrophic nitrogen fixation of the plants.

Abstract: This invention relates to CRISPR-Cas nucleases codon optimized for expression in plants and nucleic acid constructs encoding base editors comprising a CRISPR-Cas nuclease and a deaminase domain, wherein the nucleic acid constructs are optimized for expression in a plant. The invention further relates to methods of modifying nucleic acids using the nucleic acid constructs.

Abstract: A novel maize variety designated X13P468 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13P468 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13P468 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13P468, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13P468 are provided. Methods for producing maize varieties derived from maize variety X13P468 and methods of using maize variety X13P468 are disclosed.

Abstract: A novel maize variety designated X03P581 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X03P581 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X03P581 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X03P581, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X03P581 are provided. Methods for producing maize varieties derived from maize variety X03P581 and methods of using maize variety X03P581 are disclosed.

Abstract: Novel insecticidal proteins that are toxic to lepidopteran pests are disclosed. The DNA encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or the insecticidal proteins alone or in combination with an appropriate agricultural carrier can be used to control lepidopteran pests in various environments.

Abstract: The object of the present invention is to provide a spinach line with the novel feature of solid petioles at harvest time. As a result of developing many progeny lines having petioles in which the hollow portion is relatively small over many years, a spinach line having solid petioles at harvest time was constructed.

Abstract: The present invention relates to a transgenic plant which integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to one or more races of Puccinia graminis f. sp. tritici, such as the Ug99 group of races Puccinia graminis f. sp. tritici.

Abstract: The disclosure relates to lettuce variety NUN 09161 LTL as well as seeds and plants and heads or leaves thereof. NUN 09161 LTL is a multileaf green lettuce compact variety comprising resistance to Downy Mildew (Bremia lactucae) Isolates B1:16-37EU and Nasonovia ribisnigri Biotype Nr:0.

Abstract: A novel maize variety designated PH4DPN and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4DPN with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4DPN through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4DPN or a locus conversion of PH4DPN with another maize variety.

Abstract: A method for increasing the efficiency of homologous recombination-based gene editing in a plant according to an embodiment of the present invention includes optimizing temperature and photoperiod conditions during tissue culture of plant cells, expressing factors required for homology-directed DNA repair (HDR) and factors for increasing the HDR efficiency by using a multiple replicon, or regulating the HDR pathway or non-homologous end joining (NHEJ) pathway.