Patents Examined by Xiaoyun R Xu
  • Patent number: 11442046
    Abstract: The present invention relates to reagents suitable in the mass spectrometric determination of analyte molecules such as carbohydrates as well as adducts of such reagents and analyte molecules and applications of said reagents and adducts. Further, the present invention relates to methods for the mass spectrometric determination of analyte molecules.
    Type: Grant
    Filed: July 24, 2019
    Date of Patent: September 13, 2022
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Thomas Carell, Uwe Kobold, Dieter Heindl, Silvia Baecher, Andreas Leinenbach, Martin Rempt, Toni Pfaffeneder, Angie Kirchner, Olesea Kosmatchev, Rene Rahimoff, Sarah Schiffers, Markus Muller
  • Patent number: 11435372
    Abstract: Diagnostic analyzers with pretreatment carousels and related methods are disclosed. An example apparatus includes a first carousel that includes a first annular array of slots to receive a first vessel. The example first carousel also includes a first track of rotation about the first carousel having a first diameter and a second track of rotation about the first carousel having a second diameter smaller than the first diameter. The example apparatus includes a first diverter to move the first vessel from the first track to the second track. In addition, the example apparatus includes a second carousel coaxial with the first carousel. The example second carousel includes a second annular array of slots to receive a second vessel.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: September 6, 2022
    Assignee: Abbott Laboratories
    Inventors: Brian L. Ochranek, David C. Arnquist
  • Patent number: 11428639
    Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: August 30, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Chris T. Zimmerle, Michael J. Pugia
  • Patent number: 11428688
    Abstract: Compositions, methods, and systems for analyzing the protein corona are described herein, as well as its application in the discovery of advanced diagnostic tools as well as therapeutic targets.
    Type: Grant
    Filed: March 29, 2021
    Date of Patent: August 30, 2022
    Assignee: SEER, INC.
    Inventors: Hongwei Xia, Lyndal Hesterberg, Michael Figa, Xiaoyan Zhao, Gregory Troiano, William Manning, John Blume, Omid Farokhzad, Matthew McLean, Craig Stolarczyk, Marwin Ko, Theodore Platt
  • Patent number: 11424113
    Abstract: A method of mass spectrometry is disclosed comprising performing a plurality of experimental runs, wherein each experimental run comprises: periodically mass analysing fragment or product ions at a plurality of time intervals, wherein a delay time is provided between the start of the experimental run and the first time interval at which the fragment or product ions are mass analysed. Different delay times are provided in different ones of the experimental runs and fragment or product ions that have been analysed in the same time interval in at least one of said experimental runs and that have been analysed in different time intervals in at least one other of said experimental runs are identified as fragment or product ions of interest. These fragment or product ions are thus determined to relate to different precursor ions and are used to identify their respective precursor ions.
    Type: Grant
    Filed: June 11, 2015
    Date of Patent: August 23, 2022
    Assignee: Micromass UK Limited
    Inventor: Jason Lee Wildgoose
  • Patent number: 11410750
    Abstract: The present description provides a cancer assay panel for targeted detection of cancer-specific methylation patterns. Further provided herein are methods of designing, making, and using the cancer assay panel for the diagnosis of cancer.
    Type: Grant
    Filed: March 26, 2021
    Date of Patent: August 9, 2022
    Assignee: GRAIL, LLC
    Inventors: Samuel S. Gross, Oliver Claude Venn, Seyedmehdi Shojaee, John Beausang, Arash Jamshidi
  • Patent number: 11402387
    Abstract: The method presented herein includes acquiring a mass spectrum of a molecule that includes mass spectrum peaks corresponding to a precursor ion and fragment ions. The method also includes identifying at least a portion of the fragment ions in the mass spectrum as corresponding to one or more monomer subunit ion of the precursor ion by appending one or more of the fragment ions to an inferable constituent to produce a topology building block. The topology building block is then stored in a candidate pool as corresponding to one or more of the monomer subunit ion if the combined mass of the inferable constituent and one or more of the fragment ions satisfy a first user-defined mass tolerance. One or more candidate topology of the precursor ion is then obtained by combining a plurality of the topology building blocks that satisfy a second user-defined mass tolerance for the precursor ion.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: August 2, 2022
    Assignee: Brandeis University
    Inventor: Pengyu Hong
  • Patent number: 11398374
    Abstract: There are described a new type of sample holder for performing analyses of biological samples with mass spectrometry in MALDI mode, the process for its production and some protocols for the use of the sample holder in said technique. The sample holder, in its simplest embodiment (10), consists of a support (11) on a face (12) of which there is at least one porous deposit (13) consisting of nanoparticles of an oxide of a Group 4 metal.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: July 26, 2022
    Assignee: TETHIS S.P.A.
    Inventors: Emanuele Barborini, Simone Vinati, Marc Henrik Baumann
  • Patent number: 11385145
    Abstract: Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).
    Type: Grant
    Filed: September 13, 2019
    Date of Patent: July 12, 2022
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Timothy Wiles, John D. Mantlo
  • Patent number: 11378581
    Abstract: The present invention provides a method for the determination of the monoisotopic mass of a macromolecule from a mass Mmono spectrometry spectrum of said macromolecule based on the experimentally determined most abundant mass, with accuracy in the low parts-per-million (ppm) range. The method uses a simple, double-linear model for predicting the monoisotopic mass based on the experimentally determined most abundant mass, comprising the steps of (a) deriving the most abundant mass MMostAb from the spectrum; and (b) calculating the monoisotopic mass MMono from the most abundant mass MMostAb, using MMono=a+?MMOSTAB+?; wherein ? is a scalar slope obtainable by fitting the slope of monoisotopic mass versus most abundant mass for a plurality of macromolecules from a macromolecule database; and ? is a scalar residue of the form ?=?int+sfrac, ?int being an integer, and ?frac being a sawtooth function of MMostAb.
    Type: Grant
    Filed: October 20, 2017
    Date of Patent: July 5, 2022
    Assignees: VITO NV, Universiteit Antwerpen
    Inventors: Dirk Valkenborg, Jef Hooyberghs, Frank Sobott, Kris Laukens, Frederik Lermyte
  • Patent number: 11370756
    Abstract: The present disclosure relates to sulfoxide-based reagents suitable in the mass spectrometric determination of analyte molecules such as peptides as well as adducts of such reagents and analyte molecules and applications of the reagents and adducts. Further, the present disclosure relates to methods for the mass spectrometric determination of analyte molecules.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: June 28, 2022
    Assignee: Roche Diagnostics Operations, Inc.
    Inventor: Thomas Carell
  • Patent number: 11371918
    Abstract: In mass spectrometry significant error is introduced during sample preparation (sample-to-sample error), during ion generation (ion suppression), and during ion transmission (ion transmission losses). We demonstrate the ability to correct for ion suppression and ion transmission losses, and that once corrected for ion losses, a sample-to-sample normalization of the analytical sample to the internal standard is possible. By normalizing to a standard sample the analytical sample becomes completely comparable to any similarly treated sample.
    Type: Grant
    Filed: June 24, 2019
    Date of Patent: June 28, 2022
    Assignee: Iroa Technologies, LLC
    Inventors: Christopher William Ward Beecher, Felice de Jong
  • Patent number: 11367605
    Abstract: A method of ion imaging is disclosed that includes automatically sampling a plurality of different locations on a sample using a front device which is arranged and adapted to generate aerosol, smoke or vapour from the sample. Mass spectral data and/or ion mobility data corresponding to each location is obtained and the obtained mass spectral data and/or ion mobility data is used to construct, train or improved a sample classification model.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: June 21, 2022
    Assignee: MICROMASS UK LIMITED
    Inventors: Emrys Jones, Steven Derek Pringle, Zoltan Takats
  • Patent number: 11360059
    Abstract: A system and method for sample analysis using a portable gas chromatography (GC)-mass spectrometry (MS) is provided. The GC-MS system includes an injector configured to accept a sample containing a mixture of chemicals and release at least part of the sample for a separation by GC, a MEMS GC column with an integrated heater configured to accept and at least partly separate the mixture of chemicals, and a mass analyzer in a vacuum chamber configured to accept and mass-analyze the released separated chemicals. The MEMS GC column with the integrated heater is located mostly inside the MS vacuum chamber.
    Type: Grant
    Filed: May 31, 2019
    Date of Patent: June 14, 2022
    Assignee: Mass Tech, Inc.
    Inventors: Vladimir M. Doroshenko, Victor V. Laiko
  • Patent number: 11360058
    Abstract: A method of quantifying a target compound includes applying an oxidation/reduction potential to an electrochemical cell (14); measuring an electrochemical current during the application of the oxidation/reduction potential; and ionizing and directing the target compound before and after the application of the oxidation/reduction potential to a mass spectrometer (16) that measures a target compound ion intensity. The method further includes determining a target compound ion intensity change due to the application of the oxidation/reduction potential and determining a total amount of the target compound in the sample using the measured electrochemical current and the target compound ion intensity change. Determining the target compound ion intensity change may comprise either comparing the target compound ion intensity before and after the electrolysis relative to a reference peak or comparing the integrated peak area of a target compound ion in an extracted ion chromatogram before and after the electrolysis.
    Type: Grant
    Filed: October 25, 2017
    Date of Patent: June 14, 2022
    Assignee: Ohio University
    Inventor: Hao Chen
  • Patent number: 11360082
    Abstract: A detection kit is suitable for detecting a target compound. The detection kit includes a reaction container, an inspection solution composed of a hydrophobic solvent, and a plurality of fluorescent materials. The inspection solution is disposed in the reaction container. The fluorescent materials are dispersed in the inspection solution. The fluorescent material emits fluorescence, and after the fluorescent materials interact with the target compound, the intensity of the fluorescence emitted by the fluorescent materials is reduced.
    Type: Grant
    Filed: June 12, 2019
    Date of Patent: June 14, 2022
    Assignee: National Taiwan University
    Inventors: Huan-Tsung Chang, Yao-Te Yen, Yu-Shen Lin, Yu-Syuan Lin
  • Patent number: 11346844
    Abstract: Methods and apparatuses for the identification and/or characterization of properties of a macromolecule based on mass spectrometry data. Specifically, described herein are methods and apparatuses for converting peptide-level data into a pseudo-intact mass spectra. Also described herein are methods and apparatuses for converting peptide-level data into a pseudo-electropherogram. The methods may be well suited for analyzing proteins and protein complexes, including estimating properties of post-translational modifications of the proteins and protein complexes. Methods may include generating a theoretical graph or spectrum based on peptide-level mass spectrometry data. In some embodiments, the theoretical graph may be a theoretical intact mass spectrum or a theoretical charge distribution spectrum.
    Type: Grant
    Filed: April 27, 2020
    Date of Patent: May 31, 2022
    Assignee: Protein Metrics Inc.
    Inventors: Andrew Nichols, Marshall Bern, Yong Joo Kil, Eric Carlson
  • Patent number: 11333669
    Abstract: The invention provides mass spectrometry methods, compositions and systems which enable a unique platform for analyte quantitation accessing very high degrees of multiplexing and accurate quantification, particularly well-suited for a range of quantitative analysis for proteomics applications. Embodiments of the present methods and systems combine isotopic coding agents characterized by very small differences in molecular mass with mass spectrometry methods providing large resolving power to provide relative or absolute analyte quantification in a large number of samples.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: May 17, 2022
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Joshua J. Coon, Alex Hebert
  • Patent number: 11335545
    Abstract: A method of ion imaging is disclosed that includes automatically sampling a plurality of different locations on a sample using a front device which is arranged and adapted to generate aerosol, smoke or vapour from the sample. Mass spectral data and/or ion mobility data corresponding to each location is obtained and the obtained mass spectral data and/or ion mobility data is used to construct, train or improved a sample classification model.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: May 17, 2022
    Assignee: MICROMASS UK LIMITED
    Inventors: Emrys Jones, Steven Derek Pringle, Zoltan Takats
  • Patent number: 11307196
    Abstract: Apparatus and methods are described for determining a property of a biological sample. A sample carrier (22) includes one or more sample chambers (40) that define at least a first region (42) and a second region (44), the height of the one or more sample chambers varying between the first and second regions in a predefined manner. A computer processor (28) receives data relating to a first optical measurement that is performed upon a portion of the sample that is disposed within the first region, receives data relating to a second optical measurement that is performed upon a portion of the sample that is disposed within the second region, and determines the property of the sample by using a relationship between the first optical measurement, the second optical measurement, and the predefined variation in height between the regions. Other applications are also described.
    Type: Grant
    Filed: May 11, 2017
    Date of Patent: April 19, 2022
    Assignee: S.D. Sight Diagnostics Ltd.
    Inventors: Joseph Joel Pollak, Sarah Levy Schreier, Yochay Shlomo Eshel, Amir Zait, Sharon Pecker, Trevor Ruggiero