Abstract: A system and method is provided for validating the manufacturing process for the production of complex biological compositions, and particularly for providing process validation information for evaluation by a federal regulatory agency. The system and method continuously and chronologically assess the concentration of proteoforms within the biological composition as it is being produced in a fermentor. Samples from the fermentor are analyzed in a pre-selected array of analysis columns, with data generated by the columns being accumulated and evaluated, and particularly compared with data from previous stages in the production process. A continuous process validation system includes top-down and bottom-up analysis sectors, each including a plurality of different analysis columns that can be selected by the controller for a particular biological composition and a particular production process.

Abstract: Embodiments herein relate to chemical sensors based on the non-covalent surface modification of graphene with compounds containing hydrazine or hydroxylamine functional groups for the detection of aldehyde and ketone-bearing analytes. In an embodiment, a medical device is included having a graphene varactor included a graphene layer and a self-assembled monolayer disposed on an outer surface of the graphene layer through electrostatic interactions between a partial positive charge on hydrogen atoms of one or more hydrocarbons of the self-assembled monolayer and a ?-electron system of graphene. The self-assembled monolayer can include one or more compounds having one or more hydrazine groups or hydroxylamine groups, substituted hydrazine or hydroxylamine groups, or derivatives thereof. Other embodiments are also included herein.

Abstract: Compositions and methods for determining the origin location of a subterranean sample are provided. Compositions include a nanoparticle tag including a superparamagnetic iron oxide core, an intermediate layer including a fluorescent dye, and a polymer shell. The nanoparticles can be synthesized by functionalizing a superparamagnetic iron oxide nanoparticle core and covalently bonding a fluorescent dye to the functionalized nanoparticle core. In some implementations, a polymer is covalently bonded to the functionalized, fluorescent superparamagnetic iron oxide nanoparticle core. The nanoparticle tag can be used to determine the origin location of a subterranean sample by mixing the nanoparticle tag into a fluid, flowing the fluid into a subterranean formation, recovering subterranean samples from the subterranean formation, and separating tagged samples from untagged samples using a magnet.

Abstract: Methods and apparatuses for the identification and/or characterization of properties of a macromolecule based on mass spectrometry data. Specifically, described herein are methods and apparatuses for converting peptide-level data into a pseudo-intact mass spectra. Also described herein are methods and apparatuses for converting peptide-level data into a pseudo-electropherogram. The methods may be well suited for analyzing proteins and protein complexes, including estimating properties of post-translational modifications of the proteins and protein complexes. Methods may include generating a theoretical graph or spectrum based on peptide-level mass spectrometry data. In some embodiments, the theoretical graph may be a theoretical intact mass spectrum or a theoretical charge distribution spectrum.

Abstract: Apparatus and methods for sample acquisition, including for example, samples for flow cytometry systems. Certain embodiments include a plurality of plates, valves, and conduits. In particular embodiments, the plates are stacked and the conduits extend through stack of plates, and in specific embodiments each valve is in fluid communication with a conduit.

Abstract: Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.

Abstract: The present disclosure relates to novel and improved methods of analyzing proteins, peptides and polypeptides by mass spectrometry using ion-ion reactions. More specifically the disclosure relates to improved methods for implementing the m/z selective arresting of ion-ion reactions within the ion-ion reaction cell of a mass spectrometer system during a period where ion-ion reactions are performed.

Abstract: The invention generally relates to methods and devices for synchronization of ion generation with cycling of a discontinuous atmospheric interface. In certain embodiments, the invention provides a system for analyzing a sample that includes a mass spectrometry probe that generates sample ions, a discontinuous atmospheric interface, and a mass analyzer, in which the system is configured such that ion formation is synchronized with cycling of the discontinuous atmospheric interface.

Abstract: A method is for determining the effect of a selected treatment administered to a patient. The method includes acquiring VOC-emission-data of pre-treatment-target-cells-cultures and producing a pre-treatment-target cells-VOC-profile from the VOC-emission of the pre-treatment-target-cells-cultures. An MCD-target-cells-VOC-profile can be produced by inducing MCD on non-treated target-cells-cultures thereby producing post-MCD-target-cells-cultures and acquiring VOC-emission-data of the post-MCD-target-cells-cultures. The selected treatment is applied to target-cells-cultures and VOC-emission-data of post-treatment-target-cells-cultures is acquired.

Abstract: A method of analysis using mass and/or ion mobility spectrometry or ion mobility spectrometry is disclosed comprising: using a first device to generate aerosol, smoke or vapour from one or more regions of a first target of biological material; and mass and/or ion mobility analysing and/or ion mobility analysing said aerosol, smoke, or vapour, or ions derived therefrom so as to obtain first spectrometric data. The method may use an ambient ionisation method.

Abstract: The present disclosure provides feeder hydrogel particles that can function to support the growth, proliferation, and/or activation of a target cell in culture. The present disclosure also provides methods of culturing target cells with feeder hydrogel particles.

Abstract: The disclosure relates to methods of characterizing ampholyte compositions suitable for downstream applications such as capillary isoelectric focusing using liquid-chromatography-mass spectrometry.

Abstract: Fluorescent water soluble conjugated polymers including polycyclic aromatic comonomers are provided. The conjugated polymers can be linked to an acceptor fluorescent dye. The conjugated polymers find use in conjugates with biological substrates having applications in a variety of applications including methods of analyte detection.

Abstract: Provided herein are methods for determining the serotype of a virus particle and/or or determining the heterogeneity of a virus particle (e.g., an AAV particle). In other embodiments, the invention provides methods to determine the heterogeneity of AAV particles. In some aspects, the invention provides viral particles (e.g., rAAV particles) with improved stability and/or improved transduction efficiency by increasing the acetylation and/or deamidation of capsid proteins.

Abstract: The present disclosure provides a method for increasing a number of proteins identified, including: S1. adding a binding buffer and an aluminosilicate zeolite to a sample to be tested to obtain a first suspension; S2. incubating the first suspension, subjecting an incubated first suspension to first high-speed centrifugation to obtain a first supernatant and a first precipitate, discarding the first supernatant, and retaining the first precipitate; S3. repeating a process as follows several times: resuspending the first precipitate with a washing buffer to obtain a second suspension, subjecting the second suspension to second high-speed centrifugation to obtain a second supernatant and a second precipitate, discarding the second supernatant, and retaining the second precipitate; and S4. preparing the second precipitate into a protein sample, and subjecting the protein sample to mass spectrometry detection.

Abstract: A sample identification system for an automated sampling device is described. A system embodiment includes, but is not limited to, a sample holder having a plurality of apertures configured to receive a plurality of sample vessels therein, the sample holder having one or more corresponding sample holder identifiers positioned proximate to the sample holder; and an identifier capture device configured to detect the one or more sample holder identifiers positioned proximate to the sample holder and generate a data signal in response thereto, the data signal corresponding to at least an orientation of the sample holder relative to a surface on which the sample holder is positioned.

Abstract: The embodiments herein provide methods to analyze the in vitro stability of recalcitrant or membrane-bound proteins in simulated gastric fluid (SGF) comprising the proteolytic enzyme, pepsin, and in combination with a novel pepsin-trypsin assay employing state-of-the-art mass spectrometric approaches, such as LC-MS/MS, to monitor the precise degradation products. The extent of protein digestion can be evaluated by the appearance of peptic products and the disappearance of tryptic peptide products (as a proxy for intact protein). The embodiments herein also provide methods for protein quantitation using high-sensitivity LC-MRM-MS quantification. The methods embodied herein are particularly useful in charactering proteins produced in transgenic plants, such as canola genetically engineered to produce long chain omega-3 polyunsaturated fatty acids.

Abstract: Provided is an accurate, minimally invasive method for diagnosing dementia or mild cognitive impairment. The method for examining the likelihood of mild cognitive impairment or dementia, or the risk of dementia includes the steps of: measuring the amount of stereoisomers of proline in the biological sample collected from a subject; and comparing the level of D-proline with a reference value.

Abstract: Embodiments described herein generally relate to technologies for analyzing peptide structures for diagnosing and/or treating a disease state advancing through a disease progression. A non-limiting example of a method relating to the technologies described in the subject application may include receiving peptide structure data corresponding to the biological sample obtained from the subject, identifying a peptide structure profile, and diagnosing a disease state within a disease progression. The example may further include generating a diagnosis output relating to the disease state. In at least some cases, the peptide structure profile may include glycosylated peptides, aglycosylated peptides, or both.

Abstract: An analysis method incudes: preparing a sample containing microorganisms; placing the microorganisms under a first condition and then performing a first mass spectrometry on a substance produced by the microorganisms; and obtaining information on characteristics or classifications of the microorganisms contained in the sample on a basis of a difference between a first data obtained in the first mass spectrometry and a second data obtained when a second mass spectrometry is performed on a substance produced by the microorganisms after having been placed under a second condition as well as a reference data in which classifications or characteristics of the microorganisms are associated with the data obtained in the mass spectrometry of the microorganisms, in which the first condition and the second condition differ in a sugar concentration or an oxygen concentration in an environment in which the microorganisms are placed.