Abstract: Disclosed herein are methods for diagnosing or prognosticating SARS-CoV-2 infection and/or COVID-19 in a subject. The methods set forth improved immunoassays, sensing platforms, and methods for detecting SARS-CoV-2 infection and re-infection.

Abstract: Disclosed are methods of quantifying multiple quality attributes, such as post translational modifications, of multiple samples in a single mass spectrometry (MS) run, including contacting two or more samples with a digesting solution under conditions sufficient to digest samples, wherein each sample is digested separately and the digesting solution is a Tris-free buffer solution; contacting each of the two or more digested samples with a specific Tandem Mass Tag (TMT) labeling reagent under conditions sufficient to label peptides within each of the digested samples with the specific TMT labeling reagent; quenching labeling of peptides within each of the two or more digested samples; combining equal volumes of the two or more labeled, digested samples into a single combined sample solution; and analyzing the single combined sample solution by targeted mass spectral analysis, thereby allowing multiple quality attributes of the two or more samples to be quantified in a single mass spectrometry (MS) run.

Abstract: Devices and methods for measuring the quantity of multiple analytes in a sample can include a device designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and machine executable instructions configured to select the proper analyte sensing element corresponding to the analyte to be measured.

Abstract: Embodiments include a reaction vessel having a reaction chamber defined by opposing first and second interior-facing surfaces of the housing; a first light absorbing layer conforming to the first interior-facing surface of the housing component; and a second light absorbing layer conforming to the second interior-facing surface of the housing component; a first energy source configured to direct light through the housing at the first light absorbing layer; and a second energy source configured to direct light through the housing at the second light absorbing layer.

Abstract: The present invention discloses methods for identifying a protein interaction between one or more first proteins and one or more further proteins comprising the steps of: exposing one or more samples comprising the proteins to at least one preselected condition for at least one preselected duration; isolating and separating at least one soluble fraction from an insoluble fraction of said one or more samples; and analyzing the at least one soluble fraction or the insoluble fraction to identify said protein interaction between one or more first proteins and one or more further proteins. Use of the method of the invention are also disclosed.

Abstract: A method for determining in a sample, by mass spectrometry, the amount of one or more analytes selected from the group consisting of N-acetylthreonine, TMAP, phenylacetylglutamine, tryptophan, creatinine, meso-erythritol, arabitol, myo-inositol, N-acetyl serine, N-acetylalanine, 3-methylhistidine, trans-4-hydroxyproline, kynurenine, urea, C-glycosyltryptophan, 3-indoxyl sulfate, pseudouridine, and combinations thereof is described. The method comprises subjecting the sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of the one or more of the analytes; measuring, by mass spectrometry, the amount of the one or more ions from each of the one or more analytes; and using the measured amount of the one or more ions to determine the amount of each of the one or more analytes in the sample. Also described is a kit comprising one or more isotopically labeled analogues as internal standards for each of the one or more analytes.

Abstract: A method for analyzing single cells by mass spectrometry includes the steps of providing a plurality of cells in a liquid medium and placing the cells and liquid medium in a single cell isolation and ejection system. Liquid medium containing a single cell is released from the single cell isolation and ejection system. The liquid medium and single cell are captured in a capture probe containing a flowing capture probe solvent. The cell is lysed by a lysis inducer in the capture probe to disperse single cell components into the medium. The lysed single cell components are transported to a mass spectrometer, where the lysed single cell components entering the mass spectrometer are spatially and temporally separated from any dispersed components of another single cell from the sample entering the mass spectrometer. Mass spectrometry is conducted on the lysed single-cell components. A system for analyzing single cells by mass spectrometry is also disclosed.

Abstract: A detection chip, a using method for the same, and a reaction system. The detection chip includes a first substrate, a micro-cavity defining layer, and a heating electrode. The micro-cavity defining layer is on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is on the first substrate and is closer to the first substrate than the micro-cavity defining layer, and is configured to heat a plurality of micro-reaction chambers. The orthographic projection of the plurality of micro-reaction chambers on the first substrate is within the orthographic projection of the heating electrode on the first substrate.

Abstract: Among other things, the present invention is related to devices and methods of performing biological and chemical assays, such as but not limited to assay related to analysis of white blood cells.

Abstract: Synthesis of a sulfoxide-containing homobifunctional cysteine-reactive mass spectrometry-cleavable cross-linker for mapping intra-protein interactions in a protein and inter-protein interactions in a protein complex is provided. Methods for mapping intra-protein interactions in a protein and inter-protein interactions in a protein complex and cross-linking mass spectrometry for identifying one or more cross-linked peptides using the cross-linker are provided.

Abstract: This document relates to methods for detecting and quantifying heavy and light chains of immunoglobulin using mass spectrometry techniques.

Abstract: The present disclosure provides methods and compositions for the determining the abundance and/or concentration of protein biomarkers in a biological sample.

Abstract: A gas sensor 10 has a measuring channel 11 with a gas inlet 12 and with a gas outlet 13, at least one receptor layer 20, a reference electrode 30 and a voltage-controlled analysis unit 50. The reference electrode 30 is capacitively coupled with the receptor layer 20. The reference electrode 30 is connected to the analysis unit 50 in an electrically conductive manner. The receptor layer 20 is formed in measuring channel 11. The measuring channel 11 forms a dielectric layer between the receptor layer 20 and the reference electrode 30. The receptor layer 20 has a support 21 and an analyte-binding layer 22. The present invention provides for the analyte-binding layer 22 to be a self-assembling monolayer (SAM).

Abstract: A biosensor for the rapid, inexpensive, quantitative, and convenient detection of SARS-CoV-2 biomarkers, methods of manufacturing, and methods of using the same, to identify a patient's prognosis and past/present SARS-CoV-2 infection status, wherein the biosensor comprises a microfluidics layer, a multimodal sensing layer comprising two or more working electrodes, and a logic circuit that may include a processor and non-transitory memory with computer executable instructions embedded thereon.

Abstract: Compositions and methods for analyzing disulfide bonds are provided. An exemplary method includes preparing peptide standards having no disulfide bonds, scrambled disulfide bond peptide standards, and native disulfide bond peptide standards according to the sequence of the region of the protein drug product that includes the disulfide bond, digesting a sample of protein drug product into peptides, separating the protein drug product peptides, analyzing the protein drug product peptides and the peptide standards, identifying scrambled and native disulfide bond peptides by retention time, and quantifying the level of scrambled disulfide bond peptides.

Abstract: Apparatus, methods and kits for detecting the contamination of a meat sample with another type of meat using parent-daughter ion transition monitoring that identifies peptides specific to a particular type of meat. The meat types detected can include pork, beef, lamb, chicken, duck and/or horse and one or more combinations thereof.

Abstract: The present invention relates to a novel use of total cellular iron, preferably under the form of ferrous iron (Fe2+), as a marker of cancer stem cells (CSCs). The invention also relates to methods using said iron marker, in particular for metastatic cancer diagnosis or treatment, for screening for compounds of interest, as well as for killing CSCs.

Abstract: The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.

Abstract: A microwave microstrip resonator apparatus including a housing; a resonator within the housing; an output conductor within the housing and spaced apart from the resonator so as to define a capacitive gap therebetween; a reaction vessel configured to reside with the capacitive gap; and a power supply coupled to the resonator whereby contents within the reaction vessel are heated when energy is supplied to the resonator by the power supply. A mass spectrometer may also be coupled to an outlet end of the reaction vessel such that the contents within the reaction vessel are, simultaneously, delivered to the mass spectrometer for analysis.

Abstract: To provide a method for discriminating a microorganism by selecting and using a marker protein capable of reproducibly and quickly discriminating a bacterial species of the genus Listeria. The method for discriminating a microorganism according to the present invention includes: a step of subjecting a sample containing a microorganism to mass spectrometry to obtain a mass spectrum; a reading step of reading a mass-to-charge ratio m/z of a peak derived from a marker protein from the mass spectrum; and a discrimination step of discriminating which bacterial species of Listeria bacteria the microorganism contained in the sample contains based on the mass-to-charge ratio m/z, in which at least one of 17 ribosomal proteins L3, L4, L23, L2, L24, L6, L18, S5, L15, S13, S11, L10, L21, L13, S9, L31, S16 is used as the marker protein and particularly at least one of 8 ribosomal proteins L24, L6, L18, L15, S9, L31, S16 among the 17 ribosomal proteins is used.