Abstract: The present invention relates a host cell comprising an expression vector comprising a nucleic acid molecule encoding a protein requiring gamma-carboxylation and associated expression control sequences and a nucleic acid molecule encoding a vitamin K epoxido reductase and associated expression control sequences and a nucleic acid molecule encoding a ?-glutamyl carboxylase and associated control sequences. The invention further relates to a method of producing a protein requiring gamma-carboxylation in high yields.
Abstract: The present invention relates to a polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated as GND) derived from a microorganism belonging to the genus Corynebacterium, said modification being substitution of the amino acid residue(s) at the position(s) corresponding to the 158th and/or the 361st amino acid(s) of the amino acid sequence shown in SEQ ID NO: 1, and having GND activity; DNA encoding the polypeptide; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a microorganism carrying the DNA on the chromosome; and a process for producing a useful substance which comprises culturing the transformant or the microorganism in a medium.
Abstract: The subject invention provides materials and methods wherein unique and advantageous combinations of gene mutations are used to direct carbon flow from sugars to a single product. The techniques of the subject invention can be used to obtain products from native pathways as well as from recombinant pathways. In preferred embodiments, the subject invention provides new materials and methods for the efficient production of acetate and pyruvic acid.
Type:
Grant
Filed:
September 22, 2008
Date of Patent:
July 12, 2011
Assignee:
University of Florida Research Foundation, Inc.
Inventors:
Thomas B. Causey, Lonnie O'Neal Ingram, Keelnatham Shanmugam, Shengde Zhou
Abstract: Coexpression of a polyhydroxyalkanoic acid synthase and a either a fatty acid:acyl-CoA transferase or an acyl-CoA synthetase in cells enables the biosynthesis of polyester materials. Plasmids, bacteria, materials, and methods for the preparation of polyesters are disclosed.
Type:
Grant
Filed:
June 14, 2004
Date of Patent:
June 28, 2011
Assignee:
Metabolix, Inc.
Inventors:
Silke Hein, Brigitte Sohling, Gerhard Gottschalk, Alexander Steinbuchel
Abstract: The present invention provides a novel genetically modified Escherichia coli JM109 bearing accession number PTA 1579, containing the gene coding for poly-beta-hydroxybutyrate synthesis and a method of using this bacterium to produce poly-beta-hydroxybutyrate to the extent of 60% or more of the cell weight.
Type:
Grant
Filed:
November 19, 2003
Date of Patent:
November 14, 2006
Assignee:
Council of Scientific & Industrial Research
Inventors:
L. H. Mahishi, G. Tripathi, T. V. N. Ramchander, Shuban Kishen Rawal
Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.
Type:
Grant
Filed:
December 16, 2002
Date of Patent:
August 8, 2006
Assignee:
Bristol-Myers Squibb Company
Inventors:
Steven L. Goldberg, Paul M. Cino, Ramesh N. Patel, Venkata B. Nanduri, Robert M. Johnston
Abstract: The present invention relates to a novel glucose-6-phosphate dehydrogenase (hereinafter referred to as “G6PD”) derived from a bacterium belonging to the genus Corynebacterium, a DNA encoding the enzyme, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a transformant comprising the DNA on its chromosome, and a process for producing L-amino acid or G6PD which comprises culturing the transformant. According to the present invention, a modified G6PD and a DNA encoding the G6PD are obtained, and the productivity of L-amino acid by a microorganism can be improved by using the modified G6PD.
Abstract: In this paper, we describe RT-PCR strategies that allowed us to identify and clone members of the NEP-like family. Degenerate oligoncleotide primers corresponding to consensus sequences located on either side of the HEXXH consensus sequence for zincins were designed and used in RT-PCR with mouse and human testis cDNAs. DNA fragments with lengths expected from the sequence of this class of enzympes were obtained. These DNA fragments were cloned and sequenced. Using this PCR strategy and the PCR fragments as probes to screen cDNA libraries, three zincin-like peptidases were identified in addition of known members of the family. The cDNA sequences allowed to derive specific probes for Northern and in situ hybridization, and probe human chromosomes to localize the gene and establish potential links to genetic diseases. Furthermore, these cDNA sequences were used to produce recombinant fusion proteins in Escherichia coli in order to raise specific antibodies.
Abstract: Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ?-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
Type:
Grant
Filed:
May 3, 2002
Date of Patent:
June 20, 2006
Assignee:
Cognis Corporation
Inventors:
C. Ron Wilson, David L. Craft, L. Dudley Eirich, Mark Eshoo, Krishna M. Madduri, Cathy A. Cornett, Alfred A. Brenner, Maria Tang, John C. Loper, Martin Gleeson
Abstract: The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the phospholipase peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the phospholipase peptides, and methods of identifying modulators of the phospholipase peptides.
Type:
Grant
Filed:
August 13, 2003
Date of Patent:
June 13, 2006
Assignee:
Applera Corporation
Inventors:
Karl Guegler, Ellen M. Beasley, Karen A. Ketchum, Valentina Di Francesco
Abstract: Genes have been isolated from Methylomonas 16a sp. encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from a Methylomonas strain that is capable of utilizing single carbon (C1) substrates as energy sources. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.
Type:
Grant
Filed:
November 3, 2003
Date of Patent:
June 6, 2006
Assignee:
E. I. du Pont de Nemours and Company
Inventors:
Qiong Cheng, Mattheos Koffas, Kelley C. Norton, James M. Odom, Stephen K. Picataggio, Andreas Schenzle, Jean-Francois Tomb, Pierre E. Rouviere
Abstract: Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the ?-hydroxylase complex of C. tropicalis 20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.
Type:
Grant
Filed:
May 3, 2002
Date of Patent:
May 23, 2006
Assignee:
Cognis Corporation
Inventors:
C. Ron Wilson, David L. Craft, L. Dudley Eirich, Mark Eshoo, Krishna M. Madduri, Cathy A. Cornett, Alfred A. Brenner, Maria Tang, John C. Loper, Martin Gleeson
Abstract: Modified water-soluble glucose dehydrogenases having pyrrolo-quinoline quinone as a coenzyme are provided wherein at least one amino acid residue is replaced by another amino acid residue in a specific region. Modified water-soluble PQQGDHs of the present invention have improved affinity for glucose.
Abstract: The invention relates to a novel peroxidase enzyme with high dye degradation activity, the genetic information thereof and a method for degrading and decolorizing dye by using the same. The invention enables the degradation and decolorizing of a wide range of dye types in an efficient manner with no occurrence of any problem, such as secondary pollution due to the generation of hazardous byproducts or the discharge of the greenhouse effect gas due to high-level energy consumption. In accordance with the invention, further, the enzyme can be supplied at a large quantity, on the basis of the genetic information, so the enzyme can be applied to the treatment of wastewater containing dyes and the like, in the fields of staining industry and the like.
Abstract: The present invention relates to a method of assaying modulators of the interaction between Wolframin protein and its identified cellular binding partner.
Abstract: The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them. A polypeptide having the following physicochemical properties (1) to (5): (1) Action: It asymmetrically reduces N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol with NADPH as a coenzyme; (2) Optimum action pH: 4.5 to 5.5; (3) Optimum action temperature: 40° C. to 45° C.; (4) Molecular weight: About 29,000 as determined by gel filtration analysis, about 35,000 as determined by SDS-polyacrylamide gel electrophoresis analysis; (5) Inhibitor: It is inhibited by the divalent copper ion.
Abstract: This invention identifies a novel family of bilin reductases. Designated herein HY bilin reductases, the enzymes of this invention are useful in a wide variety of contexts including but not limited to the conversion of biliverdins to phytobilins and the assembly of holophytochromes or phytofluors.
Type:
Grant
Filed:
May 29, 2001
Date of Patent:
April 25, 2006
Assignee:
The Regents of the University of California
Inventors:
John Clark Lagarias, Takayuki Kochi, Nicole Frankenberg, Gregory A. Gambetta, Beronda L. Montgomery
Abstract: The present invention relates to a modified enzyme of a non-heme iron (II) dependent family of oxygenases and oxidases which renders the enzyme dependent on bicarbonate for activity. In a preferred embodiment, the modification is an arginine, lysine, or other amino acid that is two amino acid residues upstream of a histidine residue that is an iron ligand in the enzyme and is one of the histidine residues of the 2-histidine-1-aspartic acid trifacial motif. In particular, the modified enzymes are isopenicillin N synthetase, deacetoxycephalosporin C synthetase, and deacetoxycephalosporin C synthetase/deacetylcephalosporin C synthetase which are used to make antibiotics. The present invention further provides a method for making antibiotics using a modified enzyme such as isopenicillin N synthetase, deacetoxycephalosporin C synthetase, and deacetoxycephalosporin C synthetase/deacetylcephalosporin C synthetase wherein the modification renders the enzyme dependent on bicarbonate for activity.
Type:
Grant
Filed:
August 8, 2001
Date of Patent:
April 18, 2006
Assignee:
Board of Trustees of Michigan State University
Inventors:
David R. Dilley, Dina K. Kadyrzhanova, Zhenyong Wang, Toni M. Warner
Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.
Type:
Grant
Filed:
December 18, 2002
Date of Patent:
April 18, 2006
Assignee:
Ajinomoto Co., Inc.
Inventors:
Mikiko Suga, Yoko Asakura, Masakazu Sugimoto, Hisao Ito