Patents Examined by Young J. Kim
  • Patent number: 12005447
    Abstract: An apparatus and method for bio-particle detection are provided. The apparatus for bio-particle detection includes: a bio-particle detection chip including a substrate having a plurality of through-hole groups, each through-hole group of the plurality of through-hole groups including through-holes which pass through the substrate from a first surface of the substrate toward an second surface of the substrate opposite to the first surface, and which are configured to accommodate a sample solution loaded therein; and a processor configured to determine a number of through-holes, among the through holes of at least one through-hole group of the plurality of through-hole groups, having a target material encapsulated therein, based on at least one of an electrical signal and an optical signal corresponding to the through-holes of the at least one through-hole group, and to estimate a concentration of the target material based on the determined number.
    Type: Grant
    Filed: August 16, 2022
    Date of Patent: June 11, 2024
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Won Jong Jung, Hyeong Seok Jang, Kak Namkoong, Hyung Jun Youn, Jae Hong Lee
  • Patent number: 12006544
    Abstract: The identification of mutations that are present in a small fraction of DNA templates is essential for progress in several areas of biomedical research. Though massively parallel sequencing instruments are in principle well-suited to this task, the error rates in such instruments are generally too high to allow confident identification of rare variants. We here describe an approach that can substantially increase the sensitivity of massively parallel sequencing instruments for this purpose. One example of this approach, called “Safe-SeqS” for (Safe-Sequencing System) includes (i) assignment of a unique identifier (UID) to each template molecule; (ii) amplification of each uniquely tagged template molecule to create UID-families; and (iii) redundant sequencing of the amplification products. PCR fragments with the same UID are truly mutant (“super-mutants”) if ?95% of them contain the identical mutation.
    Type: Grant
    Filed: November 27, 2023
    Date of Patent: June 11, 2024
    Assignee: The Johns Hopkins University
    Inventors: Bert Vogelstein, Kenneth W. Kinzler, Nickolas Papadopoulos, Isaac A. Kinde
  • Patent number: 11998912
    Abstract: A tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet microfluidic chip; a valve control system connected to the tubing-free, sample-to-droplet microfluidic chip; a vacuum system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip; and a droplet formation pressure system fluidly connected to the tubing-free, sample-to-droplet microfluidic chip. A microfluidic chip for a tubing-free, sample-to-droplet microfluidic system includes a tubing-free, sample-to-droplet interface section; a droplet mixing section in fluid connection with the tubing-free, sample-to-droplet interface section to received droplets therefrom; an incubation section in fluid connection with the droplet mixing section to receive droplets therefrom; and a detection section in fluid connection with the incubation section to receive droplets therefrom.
    Type: Grant
    Filed: September 27, 2022
    Date of Patent: June 4, 2024
    Assignee: The Johns Hopkins University
    Inventors: Tza-Huei Jeff Wang, Kuangwen Hsieh, Yi-Fan Frank Hsieh, Aniruddha Mrithinjay Kaushik, Fangchi Shao, Pengfei Zhang
  • Patent number: 11999996
    Abstract: A method of detecting a target polynucleotide sequence in a given nucleic acid analyte characterised by the steps of: a. annealing the analyte to a single-stranded probe oligonucleotide A0 to create a first intermediate product which is at least partially double-stranded and in which the 3? end of A0 forms a double-stranded complex with the analyte target sequence; b. pyrophosphorolysing the first intermediate product with a pyrophosphorolysing enzyme in the 3?-5? direction from the 3? end of A0 to create partially digested strand A1 and the analyte; c. (i) annealing A1 to a single-stranded trigger oligonucleotide B and extending the A1 strand in the 5?-3? direction against B; or (ii) circularising A1 through ligation of its 3? and 5? ends; or (iii) ligating the 3? end of A1 to the 5? end of a ligation probe oligonucleotide C; in each case to create an oligonucleotide A2; d. priming A2 with at least one single-stranded primer oligonucleotide and creating multiple copies of A2, or a region of A2; and e.
    Type: Grant
    Filed: February 22, 2021
    Date of Patent: June 4, 2024
    Assignee: BIOFIDELITY LTD
    Inventors: Barnaby Balmforth, Cameron Frayling, Ana Silva-Weatherley, Magdalena Stolarek-Januszkiewicz
  • Patent number: 11987836
    Abstract: A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: May 21, 2024
    Assignee: GLOBAL LIFE SCIENCES SOLUTIONS USA LLC
    Inventors: Ryan Charles Heller, Nichole Lea Wood, Robert Scott Duthie, John Richard Nelson, Wei Gao, Michael James Rishel, Klaus Gustav Hentrich
  • Patent number: 11975325
    Abstract: An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.
    Type: Grant
    Filed: June 25, 2021
    Date of Patent: May 7, 2024
    Assignee: Illumina, Inc.
    Inventors: Sean M. Ramirez, Anmiv Prabhu, Rigo Pantoja, Michelle Higgins
  • Patent number: 11965809
    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
    Type: Grant
    Filed: November 12, 2021
    Date of Patent: April 23, 2024
    Inventors: Mark Andersen, Michael Pallas, Haopeng Wang
  • Patent number: 11959126
    Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.
    Type: Grant
    Filed: October 7, 2021
    Date of Patent: April 16, 2024
    Assignee: Handylab, Inc.
    Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
  • Patent number: 11938485
    Abstract: A heating device includes a heating element, a temperature sensor, a first heat pump element, a first heating block, a second heating block and a controller. The heating element is to receive an energy of the controller and convert the energy into a first thermal energy provided to the first heating block. A sensing result is generated by the temperature sensor according to the first thermal energy. The first heat pump element is to receive the energy of the controller for generating a temperature difference. The first thermal energy is conducted to the first heat pump element for forming a second thermal energy. The second heating block is to receive the second thermal energy. The controller correspondingly outputs the energy to the heating element and the first heat pump element according to the sensing result, and thereby controls the first thermal energy and the temperature difference.
    Type: Grant
    Filed: December 7, 2021
    Date of Patent: March 26, 2024
    Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    Inventor: Chien-Chih Kuo
  • Patent number: 11935626
    Abstract: The disclosure provides methods to assemble genomes of eukaryotic or prokaryotic organisms. The disclosure further provides methods for haplotype phasing and meta-genomics assemblies.
    Type: Grant
    Filed: October 9, 2020
    Date of Patent: March 19, 2024
    Assignee: The Regents of the University of California
    Inventors: Richard E. Green, Jr., Liana F. Lareau
  • Patent number: 11926869
    Abstract: The development of a simple sequence repeat (SSR) core primer group based on the whole genome sequence of pomegranate and the applications thereof are disclosed. The primer group includes 11 primer pairs: PG080, PG130, PG139, PG152, PG153, PG140, PG098, PG070, PG077, PG090, and PG093. The SSR core primer group of the present disclosure has the advantages such as high polymorphism, good repeatability, stable amplification, and clear and easy to score bands, and is applicable to the fields of pomegranate variety identification, DNA fingerprinting construction, genetic diversity assessment and phylogenetic study, and the like, providing a new tool for pomegranate molecular marker-assisted selection and having an excellent application prospect.
    Type: Grant
    Filed: July 30, 2022
    Date of Patent: March 12, 2024
    Assignee: INSTITUTE OF HORTICULTURE RESEARCH, ANHUI ACADEMY OF AGRICULTURAL SCIENCE
    Inventors: Gaihua Qin, Yiliu Xu, Chunyan Liu, Jiyu Li
  • Patent number: 11920186
    Abstract: Methods for imaging are described, including, but not limited to a method comprising: (1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand, and wherein target-specific binding partners of different specificity are linked to different docking strands, (2) optionally removing unbound target-specific binding partners, (3) contacting the sample with antigen-bound imager strands and antigen-specific binding partners linked (directly or indirectly) to optical labels, wherein the antigen-bound imager strands have complementarity to a docking strand, directly or indirectly, and wherein each antigen-specific binding partner is linked to one or more optical labels, and wherein antigen-specific binding partners of different specificity are linked to distinct optical labels, (4) optionally removing unbound antigen-bound imager strands and/or antigen-specific binding partner
    Type: Grant
    Filed: February 10, 2022
    Date of Patent: March 5, 2024
    Assignee: Ultivue, Inc.
    Inventors: Stephanie Rae Hennek, Mael Manesse
  • Patent number: 11920192
    Abstract: A method of sequencing a nucleic acid comprising (1) generating a stream of single nucleoside triphosphates by progressive enzymatic digestion of the nucleic acid; (2) producing at least one oligonucleotide used probe by reacting, in the presence of a polymerase, at least one of the single nucleoside triphosphates with a corresponding biological probe comprising (a) a first single-stranded oligonucleotide including an exonuclease blocking-site, a restriction endonuclease recognition-site located on the 5? side of the blocking-site and including a single nucleotide capture-site e, and at least one fluorophore region and (b) a second and optionally a third single-stranded oligonucleotide each separate from the first oligonucleotide; (3) cleaving the first oligonucleotide strand of the used probe at the recognition-site with a restriction endonuclease; (4) digesting the first oligonucleotide component with an enzyme to yield fluorophores in a detectable state and (5) detecting the fluorophores released in step (
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: March 5, 2024
    Assignee: LIGHTCAST DISCOVERY LTD
    Inventors: Barnaby Balmforth, Cameron Alexander Frayling, Mark Dethlefsen
  • Patent number: 11911770
    Abstract: A generic point of care based portable device and method thereof as a platform technology for detecting pathogenic infection via nucleic acid based testing achieving sample-to-result integration, comprising the following interconnected stand-alone modules: a thermal unit for executing piece-wise isothermal reactions in a pre-programmable concomitant fashion without necessitating in-between operative intervention; a colorimetric detection unit seamlessly interfaced with smartphone-app based analytics for detecting the target analyte.
    Type: Grant
    Filed: August 2, 2022
    Date of Patent: February 27, 2024
    Assignee: INDIAN INSTITUTE OF TECHNOLOGY, KHARAGPUR
    Inventors: Nandita Kedia, Sujay Kumar Biswas, Saptarshi Banerjee, Aditya Bandopadhyay, Arindam Mondal, Suman Chakraborty
  • Patent number: 11905566
    Abstract: The present invention relates to the identification of microorganisms from an environmental sample, and in particular to the rapid identification of Listeria spp. The methods and kits described herein provide a method of detecting Listeria spp. without the need for an enrichment step.
    Type: Grant
    Filed: August 10, 2021
    Date of Patent: February 20, 2024
    Assignee: NEOGEN CORPORATION
    Inventors: John Nelson, Preetha Biswas, Lei Zhang, Mark A. Mozola, Lisa Pinkava, David Brookman, Edan Robert Hosking
  • Patent number: 11905555
    Abstract: The methods, compositions, and kits of the disclosure provide a novel approach for a whole genome, unbiased DNA analysis method that can be performed on limited amounts of DNA. can be used to analyze DNA to determine its modification status. Aspects of the disclosure relate to a method for amplifying bisulfite-treated deoxyribonucleic acid (DNA) molecules comprising: (a) ligating an adaptor to the DNA molecules, wherein the adaptor comprises a RNA polymerase promoter comprising bisulfite-protected cytosines; (b) treating the ligated DNA molecules with bisulfite; (c) hybridizing the bisulfite-treated DNA molecules with a primer; (d) extending the hybridized primer to make double stranded DNA; and (e) in vitro transcribing the double-stranded DNA to make RNA.
    Type: Grant
    Filed: November 21, 2022
    Date of Patent: February 20, 2024
    Assignee: The University of Chicago
    Inventors: Chuan He, Ji Nie, Xiao-Long Cui
  • Patent number: 11904314
    Abstract: The invention is directed to devices and methods for performing rapid low-cost bioassays in self-contained disposable cartridges that provide efficient mixing of sample and reactants under a layer of liquid wax. Some embodiments additionally use gravity assisted distribution of sample and assay reagents in conjunction with an appliance containing all necessary valves, pneumatic sources, heat sources and detection stations.
    Type: Grant
    Filed: December 21, 2020
    Date of Patent: February 20, 2024
    Assignee: Wainamics, Inc.
    Inventors: Arturo M. Escajeda, Huyen Tran, Ming X. Tan
  • Patent number: 11904315
    Abstract: The invention is directed to devices and methods for performing rapid low-cost bioassays in self-contained disposable cartridges that provide efficient mixing of sample and reactants under a layer of liquid wax. Some embodiments additionally use gravity assisted distribution of sample and assay reagents in conjunction with an appliance containing all necessary valves, pneumatic sources, heat sources and detection stations.
    Type: Grant
    Filed: January 26, 2021
    Date of Patent: February 20, 2024
    Assignee: Wainamics, Inc.
    Inventors: Arturo M. Escajeda, Huyen Tran, Ming X. Tan
  • Patent number: 11905551
    Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.
    Type: Grant
    Filed: October 22, 2021
    Date of Patent: February 20, 2024
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti, George Karlin-Neumann
  • Patent number: 11898197
    Abstract: The invention is directed to devices and methods for performing rapid low-cost bioassays in self-contained disposable cartridges that provide efficient mixing of sample and reactants under a layer of liquid wax. Some embodiments additionally use gravity assisted distribution of sample and assay reagents in conjunction with an appliance containing all necessary valves, pneumatic sources, heat sources and detection stations.
    Type: Grant
    Filed: July 29, 2021
    Date of Patent: February 13, 2024
    Assignee: Wainamics, Inc.
    Inventors: Arturo M. Escajeda, Huyen Tran, Ming X. Tan