Patents Examined by Young J. Kim
  • Patent number: 10415103
    Abstract: Provided herein are a GeXP rapid detection primer set for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus, a kit, and use thereof. Disclosed are 9 pairs of specific primer and 1 pair of universal primer, for a GeXP rapid detection primer kit for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus. Eight different human-infected subtypes HA of avian influenza virus from the nine genes of M, H1, H2, H3, H5, H6, H7, H9 and H10 can be identified simultaneously with a sensitivity of 102 copies/?L.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: September 17, 2019
    Assignee: Guangxi Veterinary Research Institute
    Inventors: Zhixun Xie, Meng Li, Zhiqin Xie, Sisi Luo, Liji Xie, Li Huang, Xianwen Deng, Jiaoling Huang, Qing Fan, Yanfang Zhang, Tingting Zeng, Sheng Wang
  • Patent number: 10392654
    Abstract: The disclosure provides methods and kits involving site-specific endonuclease guided rolling circle amplification (RCA). Nucleic acid substrates, optionally generated by hybridizing a guidance primer to a single stranded nucleic acid, are cleaved with a site-specific endonuclease. In the presence of the target site, endonuclease cleavage of the substrate generates a nucleic acid having a free 3?-hydroxyl end, which is allowed to hybridize to covalently closed circular DNA probe (“take-off probe”), and initiate a rolling circle amplification (RCA) reaction. The methods and kits may be used to detect the presence of a target nucleic acid sequence, including detection of single nucleotide polymorphisms, and may be used to assess methylation status of a desired sequence, assess zygosity and/or ploidy status. The methods and kits may also be used to detect nucleic acids associated or indicative of medical conditions or pathogenic organisms.
    Type: Grant
    Filed: July 28, 2015
    Date of Patent: August 27, 2019
    Assignee: Simply Diagnostics Inc.
    Inventor: Francesco Merante
  • Patent number: 10392657
    Abstract: A method for determining the integrity of the genome of a sample and/or the quality of a library of DNA sequences obtained by deterministic restriction site whole genome amplification can include (a) amplifying the library of DNA sequences to produce first, second, and third PCR products each of a different size from 50 bp to 1000 bp, by PCR using at least one first primer pair, one second primer pair and one third primer pair, the primer pairs each hybridizing to a DNA sequence of the library having a length from 1000 bp to 5000 bp and corresponding to a sequence of the genome located respectively on a first, second and third chromosome arm; (b) detecting the first, second and third PCR products; (c) correlating the presence of the first, second and third PCR products with the integrity of the genome of the sample and/or the quality of the library.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: August 27, 2019
    Assignees: Menarini Silicon Biosystems S.p.A., Fraunhofer-Gesellschaft Zur Förderung Der Angewandten Forschung E.V.
    Inventors: Christoph Andreas Klein, Bernhard Michael Polzer, Nicolò Manaresi
  • Patent number: 10385412
    Abstract: Disclosed are methods and kits for identifying and characterizing polynucleotide sequences in a sample which may include a heterogeneous sample. Some of the methods and kits are directed to the identification and characterization of a virus in a sample, which may include HIV capable of cause AIDS or AIDS-like symptoms. The virus may be HIV-1, and may also include drug resistant mutations. The methods may include reacting a mixture that includes, in addition to nucleic acid isolated from the sample, at least one oligonucleotide capable of specifically hybridizing to HIV nucleic acid where the oligonucleotide includes at least one non-natural base.” fu addition, the methods may include detection of one or more mutations in HIV nucleic acid that are associated with drug resistance.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: August 20, 2019
    Inventor: Michael James Moser
  • Patent number: 10329605
    Abstract: A method for detecting a low-occurrence mutation in isolated DNA adds a blocking probe to reagents during amplification of the isolated DNA. The blocking probe is an oligonucleotide complementary to wild-type DNA corresponding to the sample. The blocking probe spans a site of a suspected mutation within a region of interest in the isolated DNA. After amplification, fragments of the amplified DNA is sequenced using next generating sequencing and an output is generated to display the sequenced fragments. In some embodiments, the blocking probe is locked nucleic acid (LNA).
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: June 25, 2019
    Inventor: Maher Albitar
  • Patent number: 10308988
    Abstract: The present invention provides a corn plant designated MON88017 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: June 4, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Kim A. Beazley, Timothy R. Coombe, Mark E. Groth, Terri B. Hinchey, Jay C. Pershing, Ty T. Vaughn, Bei Zhang
  • Patent number: 10294517
    Abstract: Methods and compositions disclosed herein generally relate to determination of susceptibility to eosinophilic esophagitis, asthma, and/or allergic diseases, disorders, and/or pulmonary and/or upper gastrointestinal conditions arising therefrom and/or related thereto and the diagnosis, treatment, and/or management of eosinophilic esophagitis, asthma, and/or allergic diseases, disorders, and/or pulmonary and/or upper gastrointestinal conditions arising therefrom and/or related thereto. Embodiments of the invention relate to the association between genes and specific polymorphisms of genes with eosinophilic esophagitis. Embodiments of the invention can be used to determine and manage patient risk factors for development of eosinophilic esophagitis; this determination can then be used to diagnose eosinophilic esophagitis and to treat a patient diagnosed with eosinophilic esophagitis.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: May 21, 2019
    Inventors: Marc E. Rothenberg, Leah Kottyan, John Harley
  • Patent number: 10280473
    Abstract: The present invention provides novel and non-obvious improvements to dried blood spot testing for HIV-1 viral load useful for diagnosis and monitoring treatment progression.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: May 7, 2019
    Assignee: Abbott Molecular Inc.
    Inventors: Shihai X. Huang, Chad Dunn, John Salituro, Brian Erickson
  • Patent number: 10273538
    Abstract: The invention relates to a novel method of error-free sequencing of DNA. Further, the present invention provides for a four-part oligonucleotide, comprising a fixed sequence, a randomized sequence, a restriction nuclease recognition site and/or restriction site, and a primer binding site. The invention also relates to the use of the sequenced DNA fragments obtained by the methods of the invention in methods for DNA sequence analysis, generation of cell lineage trees or assessment of copy numbers.
    Type: Grant
    Filed: February 5, 2015
    Date of Patent: April 30, 2019
    Assignee: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
    Inventors: Christoph Klein, Stefan Kirsch, Zbigniew Tadeusz Czyz, Urs Lahrmann
  • Patent number: 10273532
    Abstract: The invention provides an ultra-rapid nucleic acid amplification method performed in a flow channel. Specifically, the invention provides a nucleic acid amplification method for performing a PCR reaction by supplying a PCR sample solution to a nucleic acid amplification device comprising a serpentine channel adapted to perform at least one PCR cycle, the nucleic acid amplification device comprising a DNA denaturation temperature zone corresponding to the curved portions at one side, an annealing temperature zone corresponding to the curved portions at the other side, and an extension temperature zone positioned between the annealing and DNA denaturation temperature zones, wherein the PCR sample solution is introduced in the form of sample plugs separated by gas into the serpentine channel using a pump, the sample solution being supplied into the channel in a state such that the solution is separated by gas into a segment corresponding to one PCR cycle or smaller segments.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: April 30, 2019
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Hidenori Nagai, Yusuke Fuchiwaki
  • Patent number: 10272409
    Abstract: Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: April 30, 2019
    Inventors: Michael E. Hogan, Sergy Lemeshko, Yuri Belosludtsev, Thomas F. Powdrill, Rahul Mitra, Joseph G. Utermohlen, Frederick H. Eggers
  • Patent number: 10246738
    Abstract: Methods for imaging are described, including, but not limited to a method comprising: (1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand, and wherein target-specific binding partners of different specificity are linked to different docking strands, (2) optionally removing unbound target-specific binding partners, (3) contacting the sample with antigen-bound imager strands and antigen-specific binding partners linked (directly or indirectly) to optical labels, wherein the antigen-bound imager strands have complementarity to a docking strand, directly or indirectly, and wherein each antigen-specific binding partner is linked to one or more optical labels, and wherein antigen-specific binding partners of different specificity are linked to distinct optical labels, (4) optionally removing unbound antigen-bound imager strands and/or antigen-specific binding partner
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: April 2, 2019
    Assignee: Ultivue, Inc.
    Inventors: Stephanie Rae Hennek, Mael Manesse
  • Patent number: 10227642
    Abstract: The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: March 12, 2019
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Ulrike Fischer, Michael Greif, Harald Sobek, Johann-Peter Thalhofer
  • Patent number: 10213785
    Abstract: Method and apparatus for amplifying a target nucleic acid sequence of a reaction mixture in a Polymerase Chain Reaction (PCR). The method includes contacting the reaction mixture with EMR frequency absorbing particles formed from a material having a transition metal, transition metal oxide or a transition metal hydroxide, or a nitride, a phosphide or an arsenide of a Group III metal doped with the transition metal or a transition metal oxide, or silicon dioxide doped with the transition metal, transition metal oxide, or transition metal hydroxide; and irradiating the EMR absorbing particles with EMR having a frequency of about 200 kHz to 500 THz to amplify the target nucleic acid sequence, wherein the Group III metal is any one of Al, Ga, and In, and the transition metal is any one of Mn, Fe, Co and Cu.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: February 26, 2019
    Inventors: Dar-Bin Shieh, Chih-Chia Huang, Chen-Min Chang, Tsung-Ju Li, Po-Yang Chang, Ming-Chi Hsieh
  • Patent number: 10208339
    Abstract: Provided herein are systems and methods for whole genome amplification and sequencing. In particular, provided herein are systems and methods for detection of nucleic acid variants (e.g., rare variants) in limited samples.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: February 19, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Alain-Albert Mir, Thomas David Schaal, Jude Dunne, Maithreyan Srinivasan
  • Patent number: 10195607
    Abstract: A microchip comprises a PCR section, a denaturing section, and an electrophoresis section. In the PCR section, a desired region in DNA is amplified. In the denaturing section, PCR amplicon of double-strand DNA is denatured into single-strand DNA. In the electrophoresis section, the PCR amplicon is separated based on the length of sequence.
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: February 5, 2019
    Assignee: NEC Corporation
    Inventors: Minoru Asogawa, Hisashi Hagiwara, Yoshinori Mishina, Yasuo Iimura
  • Patent number: 10190161
    Abstract: An apparatus for nucleic acid sequencing includes: a base-detection device in a detection site, the base-detection device being configured to detect bases of a portion of a nucleic acid strand at the detection site; and a conveying device, configured to extend the nucleic acid strand and to cause the extended nucleic acid strand to slide through the detection site along a path. The base-detection device includes a plurality of field-effect nanowire detectors, arranged along the path and each including a respective nanowire and nucleic acid probes, which are defined by respective base sequences and are fixed to the respective nanowire.
    Type: Grant
    Filed: April 1, 2015
    Date of Patent: January 29, 2019
    Inventors: Marco Angelo Bianchessi, Francesco Ferrara
  • Patent number: 10167520
    Abstract: The present invention includes a method for determining the identify of an organism or virus in a sample comprising the steps of: isolating DNA or RNA from the sample; combining the DNA or RNA directly or with one or more universal or target specific amplification primers, wherein the one or more primers are specific for one or more group of target microorganisms or virus; and amplifying the DNA, or the RNA following reverse transcription with a reverse transcriptase; and contacting the amplification product with one or more species-, organism- or virus-specific detectable marker.
    Type: Grant
    Filed: December 6, 2012
    Date of Patent: January 1, 2019
    Inventor: Scot E. Dowd
  • Patent number: 10159972
    Abstract: A fluidic testing system is presented that includes a plurality of test chambers, a plurality of inlet channels, and a fluidic network that connects the inlet channels to one or more other chambers. The plurality of test chambers are each characterized by a length and a hydraulic diameter. The length of each test chamber is aligned substantially parallel to a gravity vector. Each of the test chambers has only one opening disposed along the length of the corresponding test chamber. Additionally, each of the test chambers is coupled via its respective opening to only one of the plurality of inlet channels.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: December 25, 2018
    Assignee: Stat-Diagnostica & Innovation, S.L.
    Inventors: Rafael Bru Gilbert, Jordi Carrera Fabra, Anna Comengés Casas, José Antonio Garcia Sánchez
  • Patent number: 10160987
    Abstract: A method of cleaving DNA molecules into smaller fragments by the creation of “cleavage trigger sites” followed by enzymatic processing. In some embodiments the “cleavage trigger sites” are created by the incorporation of nucleotides having uracil bases into a DNA molecule and processing with uracil DNA glycosylase, endonuclease IV and T7 endonuclease I.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: December 25, 2018
    Inventors: Rebecca F. McClure, Tyler P. Kirwan