Patents Examined by Young J. Kim
  • Patent number: 11326215
    Abstract: The present invention provides novel methods of diagnosing and determining treatment strategies for Lyme disease and other tick-borne illnesses.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: May 10, 2022
    Inventors: Nikhat Parveen, Salvatore A. E. Marras
  • Patent number: 11293049
    Abstract: Disclosed is a method for isolating, amplifying, and sequencing infectious agents' nucleic acids from an acellular fraction of a biological fluid using detergents and nucleic acids-digesting enzymes. Also disclosed is a kit-of-parts including detergents and nucleic acids-digesting enzymes for the implementation of the methods described.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: April 5, 2022
    Assignee: PATHOQUEST
    Inventor: Éric Cabannes
  • Patent number: 11293055
    Abstract: The present invention relates to a nucleic acid detection kit and a nucleic acid detection method, which use nanoparticles. More specifically, the present invention relates to: a nucleic acid detection method comprising a step of amplifying and labeling nucleic acids, and then capturing the same by using nanoparticles and centrifuging the same; and a nucleic acid detection kit using the method. The present invention is effective since a negative or positive determination for a particular disease can be made, through the nucleic acid detection method not comprising a separation step, in a more rapid, simple, sensitive, and highly reliable manner.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: April 5, 2022
    Inventors: Sang Hyun Hwang, Ji Hyun Lim, Su Jin Gang, Do Hoon Lee
  • Patent number: 11279968
    Abstract: Methods for imaging are described, including, but not limited to a method comprising: (1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand, and wherein target-specific binding partners of different specificity are linked to different docking strands, (2) optionally removing unbound target-specific binding partners, (3) contacting the sample with antigen-bound imager strands and antigen-specific binding partners linked (directly or indirectly) to optical labels, wherein the antigen-bound imager strands have complementarity to a docking strand, directly or indirectly, and wherein each antigen-specific binding partner is linked to one or more optical labels, and wherein antigen-specific binding partners of different specificity are linked to distinct optical labels, (4) optionally removing unbound antigen-bound imager strands and/or antigen-specific binding partner
    Type: Grant
    Filed: February 12, 2019
    Date of Patent: March 22, 2022
    Assignee: Ultivue, Inc.
    Inventors: Stephanie Rae Hennek, Mael Manesse
  • Patent number: 11268146
    Abstract: The present invention relates to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, and more particularly to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, which can accurately diagnose the presence or absence of a mutation in exon 4 of BIGH3 gene, which is responsible for Avellino corneal dystrophy. The use of the primer pair and probe according to the invention can diagnose Avellino corneal dystrophy in a more rapid and accurate manner than a conventional method that uses a DNA chip or PCR.
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: March 8, 2022
    Assignee: Avellino Co., Ltd.
    Inventors: Gene Lee, Jung Kuk Yun
  • Patent number: 11266990
    Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.
    Type: Grant
    Filed: August 9, 2018
    Date of Patent: March 8, 2022
    Assignee: IMEC VZW
    Inventors: Paolo Fiorini, Tim Stakenborg, Frederik Colle
  • Patent number: 11266987
    Abstract: The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: March 8, 2022
    Assignee: HandyLab, Inc.
    Inventor: Kalyan Handique
  • Patent number: 11254960
    Abstract: Methods and compositions for the amplification of nucleic acids are disclosed. Amplification methods provided herein may be performed under isothermal conditions. Methods and compositions may include reagents such as nucleic acid polymerases, ligases, and primers.
    Type: Grant
    Filed: September 8, 2015
    Date of Patent: February 22, 2022
    Assignee: Labrador Diagnostics LLC
    Inventors: Kamila Belhocine, Pranav Patel, Josephine Lee, Aaron Richardson, Scott Tabakman
  • Patent number: 11235333
    Abstract: Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform PCR amplification. Embodiments of the invention are also compatible with quantitative Polymerase Chain Reaction (“qPCR”) processes. Microfluidic devices in accordance with the invention may contain a plurality of parallel processing channels. Fully independent reactions can take place in each of the plurality of parallel processing channels. The availability of independent processing channels allows a microfluidic device in accordance with the invention to be used in a number of ways. For example, separate samples could be processed in each of the independent processing channels. Alternatively, different loci on a single sample could be processed in multiple processing channels.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: February 1, 2022
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Morten J. Jensen, Andrea W. Chow, Colin B. Kennedy, Stephane Mouradian
  • Patent number: 11230738
    Abstract: Methods and kits for detection and quantification of EGFRvIII in the peripheral blood for monitoring the therapy of GBM patients.
    Type: Grant
    Filed: October 9, 2018
    Date of Patent: January 25, 2022
    Inventors: Olivier Rixe, El Mustapha Bahassi
  • Patent number: 11220715
    Abstract: The present invention provides gene sets the expression of which is important in the diagnosis and/or prognosis of breast cancer.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: January 11, 2022
    Assignee: Genomic Health, Inc.
    Inventors: Melody A. Cobleigh, Steven Shak, Joffre B. Baker, Maureen T. Cronin
  • Patent number: 11198122
    Abstract: A diagnostic test assembly, including: a substrate having formed therein a plurality of mutually spaced open reservoirs and fluidic channels; a deformable membrane attached to the substrate to cover the open reservoirs and fluidic channels; a rigid covering disposed over the deformable membrane, the rigid covering being configured to allow respective actuators external to the diagnostic test assembly to displace corresponding portions of the deformable membrane; wherein at least some of the portions of the deformable membrane act as pumping portions, each pumping portion being disposed over a corresponding one of the reservoirs and being configured so that when it is displaced by a corresponding actuator, it is displaced into the corresponding reservoir to pump fluid from the corresponding reservoir through at least a corresponding one of the fluidic channels; and wherein one or more of the portions of the deformable membrane act as valve portions, each valve portion being configured so that when it is displa
    Type: Grant
    Filed: July 15, 2016
    Date of Patent: December 14, 2021
    Assignee: AXXIN PTY LTD
    Inventor: William Hopper
  • Patent number: 11193167
    Abstract: Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer.
    Type: Grant
    Filed: May 9, 2019
    Date of Patent: December 7, 2021
    Inventors: Shaofeng Ding, Qiang Liu
  • Patent number: 11192103
    Abstract: The present disclosure describes systems and devices capable of providing rapid polymerase chain reaction processes. A microfluidic card is insertable into a heating assembly. The heating assembly provides separate temperature zones to the card. The card includes a channel array that traverses repeatedly through the separate temperature zones so that a reaction mixture passing through the channel is subjected to thermal cycling.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: December 7, 2021
    Assignee: University of Utah Research Foundation
    Inventor: James Trauba
  • Patent number: 11193168
    Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.
    Type: Grant
    Filed: February 12, 2020
    Date of Patent: December 7, 2021
    Assignee: Exact Sciences Development Company, LLC
    Inventors: Hatim T. Allawi, William G. Weisburg, Graham P. Lidgard, Michael W. Kaiser, Abram M. Vaccaro, Gracie Shea
  • Patent number: 11193872
    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: December 7, 2021
    Assignee: Life Technologies Corporation
    Inventors: Mark Andersen, Michael Pallas, Haopeng Wang
  • Patent number: 11186862
    Abstract: Improved multiple displacement amplification (MDA) reagents and methods are provided.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: November 30, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti, George Karlin-Neumann
  • Patent number: 11180815
    Abstract: This invention relates to prognostic signatures, and compositions and methods for determining the prognosis of cancer in a patient, particularly for colorectal cancer. Specifically, this invention relates to the use of genetic markers for the prediction of the prognosis of cancer, such as colorectal cancer based on signatures of genetic markers. In various aspects, the invention relates to a method of predicting the likelihood of long-term survival of a cancer patient, a method of determining a treatment regime for a cancer patient, a method of preparing a treatment modality for a cancer patient, among other methods as well as kits and devices for carrying out these methods.
    Type: Grant
    Filed: August 27, 2019
    Date of Patent: November 23, 2021
    Inventors: Hjalmar Nekarda, Jan Friederichs, Bernhard Holzmann, Robert Rosenberg, Anthony Edmund Reeve, Michael Alan Black, John Lindsay McCall, Yu-Hsin Lin, Robert Craig Pollock
  • Patent number: 11180798
    Abstract: A method of nucleic acid amplification involving using a first modified primer which provides protection to the amplification product from exonuclease degradation and a second primer. The method provides a double stranded nucleic acid, one strand of which is degraded by a double strand nucleic acid specific exonuclease to form a single stranded nucleic acid, which is protected from exonuclease degradation.
    Type: Grant
    Filed: July 18, 2014
    Date of Patent: November 23, 2021
    Assignee: Binx Health Limited
    Inventors: Daniel Adlerstein, David M. Pearce, Anna Dixon, Wellington Fakanya
  • Patent number: 11173490
    Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.
    Type: Grant
    Filed: July 16, 2018
    Date of Patent: November 16, 2021
    Assignees: University of Washington, Global Life Sciences Solutions Operations UK Ltd.
    Inventors: David Roger Moore, Matthew Jeremiah Misner, Andrew Arthur Paul Burns, Joshua Bishop, Lisa K. Lafleur, Maxwell Wheeler