Patents Examined by Young J. Kim
  • Patent number: 11466312
    Abstract: The present invention relates to a detection method for detecting a target RNA contained in a sample with high sensitivity by using nicking/extension chain reaction system-based isothermal nucleic acid amplification (NESBA) that uses activity of a cleavage enzyme and a DNA polymerase. The NESBA of the present invention is a new concept isothermal target RNA detection method that realizes higher amplification efficiency than the existing NASBA technology and is deemed to be utilizable as a new concept diagnosis technology that can replace conventional target RNA detection technologies.
    Type: Grant
    Filed: December 31, 2018
    Date of Patent: October 11, 2022
    Assignees: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, BIONANO HEALTH GUARD RESEARCH CENTER
    Inventors: Hyun Gyu Park, Hyo Yong Kim, Yong Ju, Jun Ki Ahn
  • Patent number: 11434537
    Abstract: Provided is a method of accurate and sensitive characterization and prognosis of colorectal cancer in a subject. The method includes obtaining a cancer tissue from the subject and determining the expression level of a miRNA and a target gene of the miRNA. Also provided is a kit for prognosis of colorectal cancer in a subject in need thereof.
    Type: Grant
    Filed: February 14, 2020
    Date of Patent: September 6, 2022
    Assignee: NATIONAL CHENG KUNG UNIVERSITY
    Inventors: Nan-Haw Chow, Chien-An Chu, Hsiao-Sheng Liu, Chung-Ta Lee
  • Patent number: 11434529
    Abstract: Described herein are assays and kits capable of specifically detecting Enterocytozoon hepatopenaei (EHP) in a nucleic acid sample. In some embodiments, the nucleic acid sample is obtained from shrimp tissue, shrimp feed, or a feed ingredient.
    Type: Grant
    Filed: February 21, 2020
    Date of Patent: September 6, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
    Inventors: Siddhartha Kanrar, Arun Dhar
  • Patent number: 11421286
    Abstract: The present invention provides a corn plant designated MON88017 and DNA compositions contained therein. Also provided are assays for detecting the presence of the corn plant MON88017 based on a DNA sequence and the use of this DNA sequence as a molecular marker in a DNA detection method.
    Type: Grant
    Filed: April 17, 2019
    Date of Patent: August 23, 2022
    Assignee: MONSANTO TECHNOLOGY LLC
    Inventors: Kim A. Beazley, Timothy R. Coombe, Mark E. Groth, Terri B. Hinchey, Jay C. Pershing, Ty T. Vaughn, Bei Zhang
  • Patent number: 11421267
    Abstract: The invention features compositions and methods that are useful for the measurement of the quantity of a nucleic acid target in a sample.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: August 23, 2022
    Assignee: Accugenomics, Inc.
    Inventor: Tom B. Morrison
  • Patent number: 11408903
    Abstract: Systems, methods, and devices for discretizing and analyzing fluidic samples are provided. In one aspect, a microfluidic array for discretizing a fluidic sample comprises one or more flow channels and a plurality of fluidic compartments in fluidic communication with the one or more flow channels. In another aspect, a system for discretizing and analyzing fluidic samples comprises a rotor assembly shaped to receive a microfluidic device.
    Type: Grant
    Filed: August 31, 2020
    Date of Patent: August 9, 2022
    Assignee: University of Washington
    Inventors: Daniel T. Chiu, Bryant S. Fujimoto, Jason E. Kreutz
  • Patent number: 11384384
    Abstract: Disclosed herein are methods, reagent kits, and compositions for performing a bisulfite conversion of DNA directly from a biological sample including a patient's urine sample or a slide-mounted FFPE tissue sample. For example, a method of performing a bisulfite conversion of DNA can comprise certain preliminary steps for processing the biological sample and transferring a portion of the processed sample into a reaction vessel containing a bisulfite mixture. The method can further comprise heating the reaction vessel containing the biological sample and the bisulfite mixture at several heating temperatures and subsequently holding the reaction vessel at a holding temperature for a holding period. The method can also comprise certain bisulfite removal steps, desulfonation steps, and removal of the desulfonation solution. A final elution step can yield the converted DNA for further downstream sequencing and analysis.
    Type: Grant
    Filed: April 15, 2022
    Date of Patent: July 12, 2022
    Inventors: Tom Cheng Xu, Kuikui Shen, Sally Li Shen, Jinlan Xu, Shilei Tang, Jusong Liang
  • Patent number: 11371091
    Abstract: Apparatuses and methods related to a point-of-care portable assay device and the use thereof with a test card are described herein. In a general embodiment, a device for monitoring a polymerase chain reaction in a fluid sample includes a vacuum source configured to pull the fluid sample through a microchannel, a current source configured to cause the polymerase chain reaction while the fluid sample is located within the microchannel, a light source configured to illuminate the polymerase chain reaction while the current source causes the polymerase chain reaction, a camera imaging device configured to record an image of the polymerase chain reaction while the light source illuminates the polymerase chain reaction, and a controller configured to analyze the image of the polymerase chain reaction and output a resulting analysis of the polymerase chain reaction.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: June 28, 2022
    Assignee: Fluxergy, Inc.
    Inventors: Ryan Alan Revilla, Roy James Heltsley, Steve Hoe Lee, Farzad Izadi Kharazi, Tej Rushikesh Patel
  • Patent number: 11369965
    Abstract: A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection subsystem. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.
    Type: Grant
    Filed: March 8, 2017
    Date of Patent: June 28, 2022
    Inventors: Ronald L. Biro, Beau Hunt
  • Patent number: 11371073
    Abstract: Provided are compositions, kits, and methods for the accurate detection of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: June 28, 2022
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Beatriz Quiñones, Bertram G. Lee, Jaszemyn C. Yambao
  • Patent number: 11365405
    Abstract: The invention is directed to methods and kits which utilize an immobilized solid support to extract the nucleic acids present in the human plasma sample Immobilized solid support further comprises: a) a pre-treated solid support material b) M-Aminophenylboronic acid (APBA), and c) levoglucosenone. The components and methods for activating solid support and extraction of nucleic acids used in the invention provide for high extraction efficiency and the resultant product is a highly purified plasma which is almost completely free of nucleic acids.
    Type: Grant
    Filed: March 19, 2020
    Date of Patent: June 21, 2022
    Assignee: ANCHOR MOLECULAR INC.
    Inventor: Yabin Lu
  • Patent number: 11351510
    Abstract: The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: June 7, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Darren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
  • Patent number: 11339424
    Abstract: The present disclosure relates to a method for amplification and quantitation of a small amount of mutation using a molecular barcode and a blocking oligonucleotide.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: May 24, 2022
    Assignee: DXOME CO., LTD.
    Inventor: Jun Hyuk Chung
  • Patent number: 11332777
    Abstract: Disclosed herein are methods, reagent kits, and compositions for performing a bisulfite conversion of DNA directly from a biological sample including a patient's urine sample or a slide-mounted FFPE tissue sample. For example, a method of performing a bisulfite conversion of DNA can comprise certain preliminary steps for processing the biological sample and transferring a portion of the processed sample into a reaction vessel containing a bisulfite mixture. The method can further comprise heating the reaction vessel containing the biological sample and the bisulfite mixture at several heating temperatures and subsequently holding the reaction vessel at a holding temperature for a holding period. The method can also comprise certain bisulfite removal steps, desulfonation steps, and removal of the desulfonation solution. A final elution step can yield the converted DNA for further downstream sequencing and analysis.
    Type: Grant
    Filed: October 28, 2021
    Date of Patent: May 17, 2022
    Assignee: URIT Medical Electronic Co., Ltd.
    Inventors: Tom Cheng Xu, Kuikui Shen, Sally Li Shen, Jinlan Xu, Shilei Tang, Jusong Liang
  • Patent number: 11326215
    Abstract: The present invention provides novel methods of diagnosing and determining treatment strategies for Lyme disease and other tick-borne illnesses.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: May 10, 2022
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: Nikhat Parveen, Salvatore A. E. Marras
  • Patent number: 11293049
    Abstract: Disclosed is a method for isolating, amplifying, and sequencing infectious agents' nucleic acids from an acellular fraction of a biological fluid using detergents and nucleic acids-digesting enzymes. Also disclosed is a kit-of-parts including detergents and nucleic acids-digesting enzymes for the implementation of the methods described.
    Type: Grant
    Filed: January 6, 2017
    Date of Patent: April 5, 2022
    Assignee: PATHOQUEST
    Inventor: Éric Cabannes
  • Patent number: 11293055
    Abstract: The present invention relates to a nucleic acid detection kit and a nucleic acid detection method, which use nanoparticles. More specifically, the present invention relates to: a nucleic acid detection method comprising a step of amplifying and labeling nucleic acids, and then capturing the same by using nanoparticles and centrifuging the same; and a nucleic acid detection kit using the method. The present invention is effective since a negative or positive determination for a particular disease can be made, through the nucleic acid detection method not comprising a separation step, in a more rapid, simple, sensitive, and highly reliable manner.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: April 5, 2022
    Assignee: NATIONAL CANCER CENTER
    Inventors: Sang Hyun Hwang, Ji Hyun Lim, Su Jin Gang, Do Hoon Lee
  • Patent number: 11279968
    Abstract: Methods for imaging are described, including, but not limited to a method comprising: (1) contacting a sample being tested for the presence of one or more targets with one or more target-specific binding partners, wherein each target-specific binding partner is linked to a docking strand, and wherein target-specific binding partners of different specificity are linked to different docking strands, (2) optionally removing unbound target-specific binding partners, (3) contacting the sample with antigen-bound imager strands and antigen-specific binding partners linked (directly or indirectly) to optical labels, wherein the antigen-bound imager strands have complementarity to a docking strand, directly or indirectly, and wherein each antigen-specific binding partner is linked to one or more optical labels, and wherein antigen-specific binding partners of different specificity are linked to distinct optical labels, (4) optionally removing unbound antigen-bound imager strands and/or antigen-specific binding partner
    Type: Grant
    Filed: February 12, 2019
    Date of Patent: March 22, 2022
    Assignee: Ultivue, Inc.
    Inventors: Stephanie Rae Hennek, Mael Manesse
  • Patent number: 11268146
    Abstract: The present invention relates to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, and more particularly to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, which can accurately diagnose the presence or absence of a mutation in exon 4 of BIGH3 gene, which is responsible for Avellino corneal dystrophy. The use of the primer pair and probe according to the invention can diagnose Avellino corneal dystrophy in a more rapid and accurate manner than a conventional method that uses a DNA chip or PCR.
    Type: Grant
    Filed: April 6, 2018
    Date of Patent: March 8, 2022
    Assignee: Avellino Co., Ltd.
    Inventors: Gene Lee, Jung Kuk Yun
  • Patent number: 11266987
    Abstract: The technology described herein generally relates to microfluidic cartridges configured to amplify and detect polynucleotides extracted from multiple biological samples in parallel. The technology includes a microfluidic substrate, comprising: a plurality of sample lanes, wherein each of the plurality of sample lanes comprises a microfluidic network having, in fluid communication with one another: an inlet; a first valve and a second valve; a first channel leading from the inlet, via the first valve, to a reaction chamber; and a second channel leading from the reaction chamber, via the second valve, to a vent.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: March 8, 2022
    Assignee: HandyLab, Inc.
    Inventor: Kalyan Handique