Patents Examined by Young Kim
  • Quantitative PCR method to enumerate DNA copy number
    Patent number: 6180349
    Abstract: The present invention provides rapid and inexpensive methods for determining the copy number of a test locus of interest. The methods generally involve monitoring the formation of amplification product using real time amplification detection systems to quantify the amount of test locus and reference loci in a test subject and the amount of test locus and reference loci in at least one control subject. The methods can be used to interrogate the copy number of loci containing simple sequence repeats. Since such sequences are ubiquitous in eukaryotic genomes, the present methods have wide-ranging applicability. The methods of the present invention can be used as diagnostic and prognostic tools and in correlating abnormal copy number values for specific loci with disease and effectiveness of different treatment options.
    Type: Grant
    Filed: May 18, 1999
    Date of Patent: January 30, 2001
    Assignee: The Regents of the University of California
    Inventors: David G. Ginzinger, Tony E. Godfrey, Ronald H. Jensen, Joe W. Gray
  • Method of determining interdomain orientation and changes of interdomain orientation on ligation
    Patent number: 6171804
    Abstract: The present invention provides methods for determining the relative orientation of the individual components of a macromolecule in solution with respect to the global molecular coordinate frame of the macromolecule. The present invention further provides methods for applying this structural information, including for rational drug design.
    Type: Grant
    Filed: July 12, 1999
    Date of Patent: January 9, 2001
    Assignee: The Rockefeller University
    Inventors: David Cowburn, Rong Xu, David Fushman
  • Non-radioactive methods for chemical cleavage sequencing and footprinting of nucleic acids
    Patent number: 6165726
    Abstract: A method of sequencing a nucleic acid is provided, comprising: a) labeling the nucleic acid with biotin; b) subjecting the nucleic acid of step a) to base specific chemical modifications; c) cleaving the nucleic acid of step b) at the modified bases with hot piperidine; d) contacting the nucleic acid of step c) with immobilized streptavidin, whereby biotin-containing fragments are bound to immobilized streptavidin and unbound fragments are washed off; e) extracting the streptavidin bound fragments of step d); f) resolving the fragments of step e) on a denaturing polyacrylamide gel; and g) staining the fragments in the gel of step f) by silver staining.
    Type: Grant
    Filed: September 21, 1999
    Date of Patent: December 26, 2000
    Assignee: UT-Battelle, LLC
    Inventor: Narayana Rao Iyyalasomayazula
  • Increased throughput analysis of small compounds using multiple temporally spaced injections
    Patent number: 6156178
    Abstract: The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.
    Type: Grant
    Filed: July 13, 1999
    Date of Patent: December 5, 2000
    Assignee: Molecular Dynamics, Inc.
    Inventors: Elaine S. Mansfield, Christine Peponnet, John S. Bashkin, Curtis R. Kautzer