Abstract: Disclosed herein include methods, compositions, and kits suitable for spectral unmixing and compensation in multi-parameter flow cytometry. Disclosed herein include reagents which comprise two antibodies conjugated to one another to form a bispecific reagent (e.g., a bispecific probe). A first antibody can have affinity for a highly expressed antigen on the surface of a cell of interest and a second antibody can have affinity for each of the antibody-dye conjugates in a multi-parameter panel. There are provided, in some embodiments, methods of using the bispecific reagent to determine spillover, perform compensation, and generate a compensation matrix.

Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.

Abstract: Aspects of the present disclosure include methods for calculating one or more cell parameters of cells in a sample. Methods according to certain embodiments include irradiating a sample comprising cells in a flow stream with frequency-modulated beams of light, measuring light from the cells in the sample, generating image data of the cells from the measured light and calculating one or more cell parameters based on the generated image data. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also described. Non-transitory computer readable storage media are also provided.

Abstract: Aspects of the present disclosure include methods for assessing morphology of cell mitochondria (e.g., for use to determine viability of cells of a sample). Methods according to certain embodiments include measuring light from cells in a sample having fluorescently-labeled mitochondria, generating images of the cell mitochondria from the measured light, calculating an image parameter from the generated images of the cell mitochondria and assessing morphology of the cell mitochondria based on the calculated image parameter. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also described. Non-transitory computer readable storage media are also provided.

Abstract: Computer-implemented methods of classifying analyte data are provided. Methods of interest include applying a regression model to determine a relationship between an initial set of analyte features and a cluster criterion, generating a sparse set from at most a portion of the initial set of the analyte features based on the relationship, generating a classification model based on the sparse set, and applying the classification model to classify the analyte data into the clusters. Systems and non-transitory computer-readable storage media configured to carry out the subject methods are also provided.

Abstract: According to a present invention embodiment, a system for controlling communications comprises one or more memories and at least one processor coupled to the one or more memories. The system receives a communication for an intended recipient. The communication includes a communication identifier for the intended recipient that serves as a destination for the communication. The communication is verified against a communication control policy of the intended recipient. The communication control policy includes at least one condition for attributes of a blockchain identity of one or more users to control communications from the one or more users to the intended recipient. Transference of the communication to the intended recipient is controlled in accordance with the communication control policy. Embodiments of the present invention further include a method and computer program product for controlling communications in substantially the same manner described above.

Abstract: A welding process may be configured to convert a substrate into a welded substrate by applying a process solvent to the substrate, wherein the process solvent interrupts one or more intermolecular force between one or more component in the substrate. The substrate may be configured as a natural fiber, such as cellulose, hemicelluloses, and silk. The process solvent may be configured as an ionic-liquid based solvent and the welded substrate may be a congealed network after the process solvent has been adequately swollen and/or mobilized the substrate. A welding process may be configured such that individual fibers of a substrate are not fully dissolved such that material in the fiber core may be left in the native state by controlling process variables. The welding process fibers may have a tenacity 10% or 20% greater or a diameter 25% less than that of a cellulosic-based yarn substrate.

Abstract: In one embodiment, a method includes one or more computing systems determining a fleet-level objective for a vehicle. The fleet-level objective is associated with instructing the vehicle to travel a route according to route criteria based on the fleet-level objective. The one or more computing systems may determine one or more of an urgency score indicative of an urgency to fulfill the fleet-level objective or a risk factor score indicative of an overall condition of the vehicle. Based on one or more of the urgency score or the risk factor score, the one or more computing systems may select a particular operating mode from a plurality of operating modes for the vehicle to operate. The one or more computing system may instruct the vehicle to operate in the particular operating mode so as to fulfill the fleet-level objective.

Abstract: A novel soybean variety, designated 5PLLA47 is provided. Also provided are the seeds of soybean variety 5PLLA47, cells from soybean variety 5PLLA47, plants of soybean 5PLLA47, and plant parts of soybean variety 5PLLA47. Methods provided include producing a soybean plant by crossing soybean variety 5PLLA47 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PLLA47, methods for producing other soybean varieties or plant parts derived from soybean variety 5PLLA47, and methods of characterizing soybean variety 5PLLA47. Soybean seed, cells, plants, germ plasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PLLA47 are further provided.

Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

Abstract: In one embodiment, a method includes determining a fleet-level objective for a vehicle associated with instructing the vehicle to travel a route according to route criteria based on the fleet-level objective. The method includes receiving a ride request from a ride requestor associated with ride criteria including a pick-up location and a drop-off location. The method includes determining that the ride requestor is a passenger for the vehicle to satisfy the fleet-level objective contingent on modifications to the ride criteria. The method includes providing incentives to the ride requestor contingent on acceptance of the modifications to the ride criteria. The method includes, after receiving the acceptance of the modifications to the ride criteria, modifying the ride criteria in accordance with the route criteria. The method includes instructing the vehicle to transport the ride requestor based on the modified ride criteria so as to fulfill the fleet-level objective.

Abstract: Dissolvable pump down devices, dissolvable pump down assemblies, and methods to propel a bottomhole assembly through a lateral section are presented. A dissolvable pump down device includes an engageable portion, and one or more expandable pieces that are initially in a first position before the dissolvable pump down device is deployed in a wellbore, and configured to expand from the first position to a second position in response to a force generated by fluid flow of fluid through the wellbore. A diameter of the expandable pieces while the expandable pieces are in the first position is less than the diameter of the one or more expandable pieces while the one or more expandable pieces are in the second position. The engageable portion and the one or more expandable pieces are configured to dissolve after the dissolvable pump down device is positioned at the desired wellbore location.

Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and accurate incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

Abstract: A power supply unit adapts based on an image to be displayed by a display panel. The power supply unit includes a controller circuit for generating a power control signal, and a power supply circuit for generating one or more power supply voltages having a waveform based the power control signal. The controller circuit receives a set of configuration parameters from a display device and generates the power control signal based on the set of configuration parameters. The configuration parameters are stored in a look-up table of the display device.

Abstract: In particular embodiments, a computing system may determine a predicted amount of ride requests for a plurality of collectively-managed vehicles and determine an availability of the collectively-managed vehicles to satisfy the predicted amount of ride requests. Subsequent to determining that the availability fails to satisfy one or more predetermined criteria for servicing the predicted amount of ride requests, the system may determine status information associated with the collectively-managed vehicles and determine, based on at least the status information, one or more minimum services for servicing one or more vehicles among the plurality of collectively-managed vehicles at one or more service centers such that the availability satisfies the one or more predetermined criteria. The system may instruct the one or more vehicles that are to receive the one or more minimum services to travel to the one or more service centers to be serviced.

Abstract: The disclosed coil-integrated housing component may include an injection-molded housing dimensioned to house a display module of a computing device. Additionally, the coil-integrated housing component may include a conductive coil integrated into the injection-molded housing to surround a periphery of the display module, wherein the conductive coil may be electronically coupled to the computing device. Various other apparatuses, systems, and methods are also disclosed.

Abstract: The present disclosure relates to systems, methods, and products for adaptive design and optimization using a physics-informed neural network (PINN). The system includes a non-transitory memory and a processor. The processor executes instructions to cause the system to: input collocation points and design parameters into the PINN to obtain an output; calculate a loss function based on a set of governing equations and the output; determine whether the PINN is convergent based on the calculated loss function; in response to the PINN being convergent, export the PINN; and in response to the PINN not being convergent: determine whether to resample the collocation points; determine an optimum number of collocation points; determine a set of optimal network parameters for adjusting the PINN; and input the collocation points and the set of optimal network parameters to the PINN for a next iteration.

Abstract: In particular embodiments, a computing system may determine a predicted amount of ride requests for a plurality of collectively-managed vehicles and determine an availability of the collectively-managed vehicles to satisfy the predicted amount of ride requests. Subsequent to determining that the availability fails to satisfy one or more predetermined criteria for servicing the predicted amount of ride requests, the system may determine status information associated with the collectively-managed vehicles and determine, based on at least the status information, one or more minimum services for servicing one or more vehicles among the plurality of collectively-managed vehicles at one or more service centers such that the availability satisfies the one or more predetermined criteria. The system may instruct the one or more vehicles that are to receive the one or more minimum services to travel to the one or more service centers to be serviced.

Abstract: Phosphoramidate-based monomers are provided for use in the synthesis of expandable polymers for nanopore-based sensing. Such monomers comprising a reporter construct that contain a first reporter code, a symmetrical chemical brancher bearing a translocation control element, and a second reporter code, wherein the ends of the reporter construct are attached to phosphoramidate-nucleoside. Related methods and products are also provided.

Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and accurate incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.