Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

Abstract: Dissolvable pump down devices, dissolvable pump down assemblies, and methods to propel a bottomhole assembly through a lateral section are presented. A dissolvable pump down device includes an engageable portion, and one or more expandable pieces that are initially in a first position before the dissolvable pump down device is deployed in a wellbore, and configured to expand from the first position to a second position in response to a force generated by fluid flow of fluid through the wellbore. A diameter of the expandable pieces while the expandable pieces are in the first position is less than the diameter of the one or more expandable pieces while the one or more expandable pieces are in the second position. The engageable portion and the one or more expandable pieces are configured to dissolve after the dissolvable pump down device is positioned at the desired wellbore location.

Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.

Abstract: Methods, compositions and devices for single molecule sequencing are provided, particularly for solid-state synthesis and processing of expandable polymers (e.g., Xpandomers), as well as methods and compositions for producing new expandable polymer constructs that provide more accurate sequence information when passed through a nanopore sensor.

Abstract: A novel soybean variety, designated 5PWGS87 is provided. Also provided are the seeds of soybean variety 5PWGS87, cells from soybean variety 5PWGS87, plants of soybean 5PWGS87, and plant parts of soybean variety 5PWGS87. Methods provided include producing a soybean plant by crossing soybean variety 5PWGS87 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PWGS87, methods for producing other soybean varieties or plant parts derived from soybean variety 5PWGS87, and methods of characterizing soybean variety 5PWGS87. Soybean seed, cells, plants, germ plasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PWGS87 are further provided.

Abstract: Disclosed herein include methods, compositions, and kits suitable for spectral unmixing and compensation in multi-parameter flow cytometry. Disclosed herein include reagents which comprise two antibodies conjugated to one another to form a bispecific reagent (e.g., a bispecific probe). A first antibody can have affinity for a highly expressed antigen on the surface of a cell of interest and a second antibody can have affinity for each of the antibody-dye conjugates in a multi-parameter panel. There are provided, in some embodiments, methods of using the bispecific reagent to determine spillover, perform compensation, and generate a compensation matrix.

Abstract: A wellbore isolation device, comprising: a mandrel 24 having a cylindrical body, a sealing element 38 disposed about the mandrel 24 and radially expandable from a first run-in diameter to sealing engagement with the an inner surface 50 of the wellbore 6; a metal backup ring 60 disposed on the mandrel 24 contacting one side of the sealing element system 38 with an initial height H and radially expandable and axially deformable to abut the inner surface 50 of the wellbore 6; wherein the metal backup ring 60 is a concave cross-section about the mandrel 24 with an uphole leg and a downhole leg; and wherein an applied compressive force expands the sealing element system 38 into sealing engagement with the wellbore 6 and the metal backup ring 60 radially expands while axially deforming to abut the inner surface 50 of the wellbore 6 with no contact pressure.

Abstract: A novel soybean variety, designated 5PFAH54 is provided. Also provided are the seeds of soybean variety 5PFAH54, cells from soybean variety 5PFAH54, plants of soybean 5PFAH54, and plant parts of soybean variety 5PFAH54. Methods provided include producing a soybean plant by crossing soybean variety 5PFAH54 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PFAH54, methods for producing other soybean varieties or plant parts derived from soybean variety 5PFAH54, and methods of characterizing soybean variety 5PFAH54. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PFAH54 are further provided.

Abstract: A novel soybean variety, designated 5PGYS00 is provided. Also provided are the seeds of soybean variety 5PGYS00, cells from soybean variety 5PGYS00, plants of soybean 5PGYS00, and plant parts of soybean variety 5PGYS00. Methods provided include producing a soybean plant by crossing soybean variety 5PGYS00 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PGYS00, methods for producing other soybean varieties or plant parts derived from soybean variety 5PGYS00, and methods of characterizing soybean variety 5PGYS00. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PGYS00 are further provided.

Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and accurate incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.

Abstract: The invention relates to methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

Abstract: The invention relates to compounds, methods and compositions for improving on nucleic acid polymerization, including DNA replication by in vitro primer extension to generate, for example, polymers for nanopore-based single molecule sequencing of a DNA template. A nucleic acid polymerase reaction composition is provided with polymerization enhancement moieties, which allows enhanced DNA polymerase activity with nucleotide analogs, resulting in improved length of primer extension products for sequencing applications.

Abstract: In one embodiment, a method includes determining a fleet-level objective for a vehicle associated with instructing the vehicle to travel a route according to route criteria based on the fleet-level objective. The method includes receiving a ride request from a ride requestor associated with ride criteria including a pick-up location and a drop-off location. The method includes determining that the ride requestor is a passenger for the vehicle to satisfy the fleet-level objective contingent on modifications to the ride criteria. The method includes providing incentives to the ride requestor contingent on acceptance of the modifications to the ride criteria. The method includes, after receiving the acceptance of the modifications to the ride criteria, modifying the ride criteria in accordance with the route criteria. The method includes instructing the vehicle to transport the ride requestor based on the modified ride criteria so as to fulfill the fleet-level objective.

Abstract: In particular embodiments, a computing system may determine a predicted amount of ride requests for a plurality of collectively-managed vehicles and determine an availability of the collectively-managed vehicles to satisfy the predicted amount of ride requests. Subsequent to determining that the availability fails to satisfy one or more predetermined criteria for servicing the predicted amount of ride requests, the system may determine status information associated with the collectively-managed vehicles and determine, based on at least the status information, one or more minimum services for servicing one or more vehicles among the plurality of collectively-managed vehicles at one or more service centers such that the availability satisfies the one or more predetermined criteria. The system may instruct the one or more vehicles that are to receive the one or more minimum services to travel to the one or more service centers to be serviced.

Abstract: In an embodiment, a power management system includes a detection unit configured to detect a power interruption to a power supply. The system further includes a communication interface configured to, in response to the detected power interruption, provide a message regarding the detected power interruption. In response to the detected power interruption, a computer network switch provides notifications to a plurality of servers connected to the switch to allow the plurality of servers to prepare for a loss in power.

Abstract: Phase modulated Optical Parametric Amplification Imaging (p-OPA), can be used to determine the magnitude and the sign of the second-order nonlinear susceptibility of a material, and its spatial variation.

Abstract: Phosphoramidate esters and related nucleotide analogs useful in polynucleotide sequencing techniques, and synthetic methods for preparing those compounds, are disclosed, including compounds having the following structure: wherein R1, R2, R3 and R4 are as defined herein. These compounds include nucleotide phosphoramidates analogs that are modified on the alpha-phosphate to enable attachment of a variety of application-specific substituents such as tether molecules.

Abstract: In one embodiment, a method includes a computing system receiving information from an autonomous vehicle (AV). Based on the received information, the system may identify a target objective, with an associated target destination, for the AV and determine that the AV is able to transport passengers while furthering the target objective. The system may receive multiple ride requests from ride requestors, respectively. Each of the ride requests is associated with an origination location and a destination location. The system may match the AV with one of the ride requests based on a location of the AV, the target destination of the target objective, and the origination location and destination location associated with the ride request. The system may instruct the AV to perform a transportation task from the origination location to the destination location associated with the ride request, and drive to the target destination after completing the transportation task.

Abstract: In particular embodiments, a computing system may generate a prediction of requests for autonomous vehicles in a fleet of collectively managed autonomous vehicles based on a current condition and a future event, the prediction including a predicted request level and a predicted duration of the request level. The system may receive status information from fleet vehicles and identify a vehicle in need of service. The system may receive status information from service centers and identify a service center to service the vehicle. The system may determine a time at which to service the vehicle at the identified service center based on the generated prediction of requests, schedule the vehicle for service at the identified service center at the determined time, and instruct the vehicle to drive to the service center to be serviced at the determined time. In particular embodiments, the prediction of requests may be generated using machine learning.

Abstract: This disclosure is related to systems, devices, processes, and methods to optimize a laser power boost amplitude, a laser power boost duration, or both in a heat-assisted data recording device, such as in heat-assisted magnetic recording (HAMR). The amplitude and duration for the laser power boost may be determined for a specific portion of a write operation, such as a first sector of the write operation. During operation of a data storage device, the laser power boost may provide additional power to the laser for the specific portion. Once the laser power boost duration has elapsed, the data storage device may continue providing power to the laser at the normal power input range of the laser. The laser power boost settings may be determined on a per head per zone basis, per track basis, or another configuration.