Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Abstract: Systems, devices, and methods are described herein for distributing syndicated content or content with one or more distribution restrictions. In one aspect, a method may include assigning a unique ID to a plurality of content items, where the unique ID of at least one of the content items is associated with one or more property distribution restrictions. The method may further include streaming in a video stream the content items to at least two properties and monitoring the unique IDs of the plurality of content items streamed in the video stream to detect least one content item associated with the one or more property distribution restrictions. The distribution of the least one content item associated with the one or more property distribution restrictions may be blocked from the one or more properties concurrently with streaming the video stream, based on the one or more property distribution restrictions.

Abstract: The present disclosure describes the engineering of microbial cells for fermentative production of 1,5-diaminopentane and provides novel engineered microbial cells and cultures, as well as related 1,5-diaminopentane production methods.

Abstract: The present invention includes a method of detecting and treating a patient suspected of having Barrett's esophagus comprising: obtaining a biological sample from an esophagus of the patient; determining a microbiome in the biological sample by detecting the presence of bacteria by plasmid dilution verification or quantitative polymerase chain reaction (qPCR); wherein if the patient has a microbiome indicative of an increased risk of esophageal cancer threating the patient with at least one of: removing at least part of the esophagus, esophagectomy probiotic therapy, or chemically targeting elimination of bacteria indicative of an increased risk of esophageal cancer.

Abstract: Apparatus and methods for processing a substrate including an injector unit insert with a plurality of flow paths leading to a first plenum, each of the flow paths providing one or more of substantially the same residence time, length and/or conductance. Injector units including the injector unit inserts have increased flow uniformity.

Abstract: A thermal and environmental noise suppressing interface circuit which is configured to operate cold and is configured to perform biasing with suppression of noise currents from room temperature noise voltages and dc coupled rf readout of a superconducting device under test with a single coaxial cable or equivalent conductor pair. The circuit is configured to suppress the propagation of thermal and environmental noises to/from sensors operating at a different temperature from its operating and control equipment while maintaining a single input-output channel, and provides for the placement of a local grounding impedance on an intercept board.

Abstract: The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.

Abstract: A seal row bench assembly includes a first bench and a second bench such that the first bench is configured to pivot about an axis relative to the second bench, an adjustable column coupled to the second bench that permits variable height of at least one of the first bench and the second bench, and a post assembly in communication with the first bench such that post assembly has one or more index locations to locate the first bench at a set angle relative to the second bench. Variations of the assembly include coupling the first bench to the second bench using a hinge and coupling the first bench to a second column using a hinge.

Abstract: Methods, compositions, and kits for high throughput DNA assembly reactions in vitro. Modular CRISPR DNA constructs comprising modular insert DNA parts flanked by cloning tag segments comprising pre-validated CRISPR protospacer/protospacer adjacent motif sequence combinations. High throughput methods of CRISPRi and CRISPRa.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Abstract: A cooking appliance including an oven body having an interior compartment and a door panel for accessing the interior compartment, wherein the interior compartment is sized to receive a food product; a removable heated tray positionable within the interior compartment, wherein the heated tray includes a top tray surface and a first heating source positioned below the top tray surface; and a second heating source positioned within the interior compartment.

Abstract: Systems, devices, and methods are described herein for distributing syndicated content or content with one or more distribution restrictions. In one aspect, a method may include assigning a unique ID to a plurality of content items, where the unique ID of at least one of the content items is associated with one or more property distribution restrictions. The method may further include streaming in a video stream the content items to at least two properties and monitoring the unique IDs of the plurality of content items streamed in the video stream to detect least one content item associated with the one or more property distribution restrictions. The distribution of the least one content item associated with the one or more property distribution restrictions may be blocked from the one or more properties concurrently with streaming the video stream, based on the one or more property distribution restrictions.

Abstract: The present disclosure relates to methods of joining three or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro or in vivo. The method allows the joining of a large number of DNA fragments, in a deterministic fashion. It can be used to rapidly generate nucleic acid libraries that can be subsequently used in a variety of applications that include, for example, genome editing and pathway assembly. Kits for performing the method are also disclosed.

Abstract: Embodiments disclosed herein describe a liner assembly including a plurality of individually separated gas passages. The liner assembly provides control of flow parameters, such as velocity, density, direction and spatial location, across a substrate being processed. The processing gas across the substrate being processed may be specially tailored for individual processes with a liner assembly according to the present embodiments.

Abstract: Systems, methods, and devices are described herein for placing secondary content into a break of unknown duration in a stream of primary content. In one aspect, the described techniques may include streaming a segment of primary content, for example, by a reduce capability client device, such as a mobile device or tablet. The device may receive information indicative of a break in the streaming of the primary content. The device may play secondary content based on receiving the information indicative of the break. The device may determine when a next segment of the primary content is available, and begin streaming the next segment of the primary content upon detecting that the second segment of the primary content is available.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.