Abstract: An improved method of in situ hybridization which relies on an improved formulation of the in situ hybridization buffer is described. In at least some formulations the buffer are non-toxic. The combination of Locked Nucleic Acid (LNA) comprising ISH probes and the improved ISH buffer are useful for detection of small non-coding RNA as well as in the manufacturing of ISH kits directed to the detection of such small non-coding RNA. Further disclosed is a method of semi-quantitative ISH and demonstration of the semi-quantitative ISHs diagnostic potential.

Abstract: A system and method for requesting, validating, and monitoring hall passes digitally.

Abstract: Embodiments relate to an off-road vehicle comprising a frame, including at least one cargo box support member, a suspension movably coupled to the frame, a passenger compartment, an engine, a transmission operatively coupled to the engine, and a cargo box. The cargo box includes a floor and a plurality of upwardly extending sidewalls, wherein at least a portion of the cargo box floor extends over the at least one cargo box support member and wherein the cargo box is removably coupled to the at least one cargo box support members and is removable from the off-road vehicle via the removal of fewer than eight fasteners.

Abstract: An improved method of in situ hybridization which relies on an improved formulation of the in situ hybridization buffer is described. In at least some formulations the buffer are non-toxic. The combination of Locked Nucleic Acid (LNA) comprising ISH probes and the improved ISH buffer are useful for detection of small non-coding RNA as well as in the manufacturing of ISH kits directed to the detection of such small non-coding RNA. Further disclosed is a method of semi-quantitative ISH and demonstration of the semi-quantitative ISHs diagnostic potential.

Abstract: A medication analysis system maintains a database of medication profiles for users of medications, each medication profile being associated with a person and includes demographic information about the person and user experience information describing the person's experiences with one or more medications. An input from a user is received that requests information about a medication for a desired demographic. A cluster of medication profiles is identified from the database of medication profiles that include user experience information describing peoples' experiences with the medication. One or more satisfaction metrics are determined for the medication using user experience information in the identified set of medication profiles.

Abstract: An improved method of in situ hybridization which relies on an improved formulation of the in situ hybridization buffer is described. In at least some formulations the buffer are non-toxic. The combination of Locked Nucleic Acid (LNA) comprising ISH probes and the improved ISH buffer are useful for detection of small non-coding RNA as well as in the manufacturing of ISH kits directed to the detection of such small non-coding RNA. Further disclosed is a method of semi-quantitative ISH and demonstration of the semi-quantitative ISHs diagnostic potential.

Abstract: An improved method of in situ hybridization which relies on an improved formulation of the in situ hybridization buffer is described. In at least some formulations the buffer are non-toxic. The combination of Locked Nucleic Acid (LNA) comprising ISH probes and the improved ISH buffer are useful for detection of small non-coding RNA as well as in the manufacturing of ISH kits directed to the detection of such small non-coding RNA. Further disclosed is a method of semi-quantitative ISH and demonstration of the semi-quantitative ISHs diagnostic potential.

Abstract: Automatically characterizing a behavior of an object or objects by processing object data to obtain a data set that records a measured parameter set for each object over lime, providing a learning input that identifies when the measured parameter set of an object is associated with a behavior, processing the data set in combination with the learning input to determine which parameters of the parameter set over which range of respective values characterize the behavior; and sending information that identifies which parameters of the parameter set over which range of respective values characterize the behavior for use in a process that uses the characteristic parameters and their characteristic ranges to process second object data and automatically identify when the behavior occurs. Also disclosed is a method of tracking one or more objects.

Abstract: A bowling lane maintenance apparatus includes a transfer roller and a dressing oil tank configured to contain dressing oil. A wick is configured to pass dressing oil from the tank to the transfer roller. A buffer brush is configured to receive dressing oil from the transfer roller and spread the dressing oil on a bowling lane. The wick may comprise a foam material having a first region having a first capillarity and a second region having a second capillarity differing from the first capillarity. The wick may also be maintained in contact with the transfer roller.

Abstract: A method of determining the position of a target comprising the steps of: providing a transmitter or a plurality of transmitters to transmit a signal to the target, providing a receiver or a plurality of receivers to receive signals reflected from said target. Determining the time of arrival information of said reflected signal at the or each receiver; using information pertaining to the position of the or each receiver and/or the or each transmitter and with the information obtained determining the target position. Preferably the signal includes a modulated or coded portion unique to the transmitter; and said signal portion is associated with the or each particular transmitter by virtue of said signal portion. The signal maybe a mobile phone transmission, DAB, digital TV, digital Radio or digital satellite transmissions.

Abstract: A CELO virus obtained by in vitro manipulation of a plasmid-cloned CELO virus DNA is suitable for the production of vectors for gene therapy and as a vaccine against infectious diseases in humans and animals, particularly birds.

Abstract: A CELO virus obtained by in vitro manipulation of a plasmid-cloned CELO virus DNA is suitable for the production of vectors for gene therapy and as a vaccine against infectious diseases in humans and animals, particularly birds.

Abstract: The toxicity problems which occur when foreign material is introduced into higher eukaryotic cells, particularly during transfection with DNA, are countered by causing gene products to be expressed in the cell, which block the apoptosis triggered by the transfection process, and/or by treating the cells with anti-inflammatory substances. Preferably, Bcl-2, E1B 19K or an anti-apoptotically active gene of chicken adenovirus CELO is used as the anti-apoptosis gene whilst the inflammatory substance used is adenovirus VA1, which is introduced into the cell in the form of VA1-DNA. Using these methods, long-lasting gene expression can be achieved.

Abstract: A CELO virus obtained by in vitro manipulation of a plasmid-cloned CELO virus DNA is suitable for the production of vectors for gene therapy and as a vaccine against infectious diseases in humans and animals, particularly birds.

Abstract: The toxicity problems which occur when foreign material is introduced into higher eukaryotic cells, particularly during transfection with DNA, are countered by causing gene products to be expressed in the cell, which block the apoptosis triggered by the transfection process, and/or by treating the cells with anti-inflammatory substances. Preferably, Bcl-2, E1B 19K or an anti-apoptotically active gene of chicken adenovirus CELO is used as the anti-apoptosis gene whilst the inflammatory substance used is adenovirus VA1, which is introduced into the cell in the form of VA1-DNA. Using these methods, long-lasting gene expression can be achieved.