Patents by Inventor Akinobu Matsuyama

Akinobu Matsuyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20120142050
    Abstract: The present inventors constructed a transformation system which uses as a host the Kocuria rhizophila NBRC 103217 strain which can maintain its cellular structure in various organic solvents and shows little protein leakage. As a result, by developing novel shuttle vectors derived from Kocuria varians NBRC 15358, the present inventors successfully induced the expression of a foreign protein in microorganisms belonging to the genus Kocuria which can maintain their cellular structure in non-aqueous systems and show little protein leakage.
    Type: Application
    Filed: January 13, 2010
    Publication date: June 7, 2012
    Applicant: Daicel Chemical Industries, Ltd
    Inventors: Eitaro Matsumura, Momoko Hamasaki, Akinobu Matsuyama
  • Patent number: 7300784
    Abstract: An objective of the present invention is to provide a nitrile hydratase capable of producing 2-hydroxy-4-methylthiobutyroamide. The present invention provides a novel nitrile hydratase producing ?-hydroxyamide using, ?-hydroxnitrile as the substrate, and the encoding DNA thereof. The enzyme can be obtained from Rhodococcus sp. Further, the enzymatic activity of the enzyme can he maintained stably during the reaction. The present invention provides a method for producing amide compounds, the method comprising the step of reacting this enzyme to nitrile compounds. According to the present invention, from hydroxy nitrile compounds, corresponding amide compounds can be produced biochemically without reducing the enzyme activity of nitrile hydratase.
    Type: Grant
    Filed: March 1, 2002
    Date of Patent: November 27, 2007
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Toru Nagasawa, Akinobu Matsuyama
  • Patent number: 6905861
    Abstract: D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.
    Type: Grant
    Filed: September 10, 2002
    Date of Patent: June 14, 2005
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
  • Patent number: 6902915
    Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).
    Type: Grant
    Filed: February 7, 2003
    Date of Patent: June 7, 2005
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Shinji Tokuyama, Akinobu Matsuyama
  • Patent number: 6900037
    Abstract: The present invention provides a method for producing amide compounds using hydroxyl nitrile compounds and microorganisms and/or enzymatically active material having cyanide-resistant nitrile hydratase activity, e.g., Rhodococcus equi XL-l. Furthermore, the amide compounds can be produced while the enzymatic activity of this microorganism can be stably maintained during the reaction.
    Type: Grant
    Filed: June 13, 2002
    Date of Patent: May 31, 2005
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Tohru Nagasawa, Akinobu Matsuyama
  • Patent number: 6887697
    Abstract: The present invention provides the D-aminoacylase-encoding gene derived from Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the gene, and the homologues thereof. The D-aminoacylase of the present invention is capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful as a medicinal raw material or the like.
    Type: Grant
    Filed: December 31, 2003
    Date of Patent: May 3, 2005
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
  • Publication number: 20040209345
    Abstract: An objective of the present invention is to provide a nitrile hydratase capable of producing 2-hydroxy-4-methylthiobutyroamide. The present invention provides a novel nitrile hydratase producing &agr;-hydroxyamide using, &agr;-hydroxnitrile as the substrate, and the encoding DNA thereof. The enzyme can be obtained from Rhodococcus sp. Further, the enzymatic activity of the enzyme can he maintained stably during the reaction. The present invention provides a method for producing amide compounds, the method comprising the step of reacting this enzyme to nitrile compounds. According to the present invention, from hydroxy nitrile compounds, corresponding amide compounds can be produced biochemically without reducing the enzyme activity of nitrile hydratase.
    Type: Application
    Filed: March 25, 2004
    Publication date: October 21, 2004
    Inventors: Tohru Nagasawa, Akinobu Matsuyama
  • Publication number: 20040166564
    Abstract: The present invention provides the D-aminoacylase-encoding gene derived from Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the gene, and the homologues thereof. The D-aminoacylase of the present invention is capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful as a medicinal raw material or the like.
    Type: Application
    Filed: December 31, 2003
    Publication date: August 26, 2004
    Applicant: Daicel Chemical Industries, Ltd., a Japan corporation
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
  • Patent number: 6780619
    Abstract: The present invention provides the D-aminoacylase-encoding gene derived from Hypomyces mycophilus, a filamentous fungus, the polypeptide encoded by the gene, and the homologues thereof. The D-aminoacylase of the present invention is capable of producing D-tryptophan from N-acetyl-D-tryptophan. D-tryptophan is useful as a medicinal raw material or the like.
    Type: Grant
    Filed: January 26, 2001
    Date of Patent: August 24, 2004
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
  • Patent number: 6699695
    Abstract: A nitrile compound having a complicated structure (e.g., 2-hydroxy-4-methylthiobutyronitrile) is converted into an amide compound with high production efficiency, by using a novel microorganism of which the gene 16S rRNA has a specific base sequence. As the microorganism, Rhodococcus sp. Cr4 strain and Rhodococcus sp. Am8 strain or the like is employed.
    Type: Grant
    Filed: August 14, 2000
    Date of Patent: March 2, 2004
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Masato Kawabe, Toru Nagasawa
  • Patent number: 6664083
    Abstract: A method for racemizing with N-acylamino acid racemase (NAAR) derived from Sebekia benihana and a method for producing optically active amino acids using the racemaization method are provided. The racemase of the present invention can efficiently catalyze the racemization of acylamino acid substrates including N-acylalanine, N-acylaspartic acid, N-acylleucine, and N-acylvaline. Furthermore, this method can be applied to efficient production of optically active amino acids, which are useful, for example, as medicinal raw materials.
    Type: Grant
    Filed: February 27, 2001
    Date of Patent: December 16, 2003
    Assignee: Daichel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Shinji Tokuyama
  • Publication number: 20030203455
    Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective , the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).
    Type: Application
    Filed: February 7, 2003
    Publication date: October 30, 2003
    Applicant: Daicel Chemical Industries, Ltd., a Japan corporation
    Inventors: Shinji Tokuyama, Akinobu Matsuyama
  • Publication number: 20030170869
    Abstract: D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.
    Type: Application
    Filed: January 17, 2003
    Publication date: September 11, 2003
    Applicant: Daicel Chemical Industries, Ltd., a Japan corporation
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
  • Publication number: 20030157665
    Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).
    Type: Application
    Filed: February 7, 2003
    Publication date: August 21, 2003
    Applicant: Daicel Chemical Industries, Ltd., a Japan Corporation
    Inventors: Shinji Tokuyama, Akinobu Matsuyama
  • Publication number: 20030143701
    Abstract: An (R)-2-amino-1-phenylethanol derivative shown by the general formula (IIa) 1
    Type: Application
    Filed: July 31, 2002
    Publication date: July 31, 2003
    Applicant: Daicel Chemical Industries, Ltd.
    Inventors: Akinobu Matsuyama, Michio Ito
  • Patent number: 6596528
    Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective, the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: July 22, 2003
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Shinji Tokuyama, Akinobu Matsuyama
  • Publication number: 20030124691
    Abstract: An objective of the present invention is to provide a method for producing amide compounds.
    Type: Application
    Filed: June 13, 2002
    Publication date: July 3, 2003
    Applicant: Daicel Chemical Industries, Ltd.
    Inventors: Tohru Nagasawa, Akinobu Matsuyama
  • Publication number: 20030113893
    Abstract: D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.
    Type: Application
    Filed: September 10, 2002
    Publication date: June 19, 2003
    Applicant: Daicel Chemical Industries, Ltd., a Japan corporation
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama
  • Publication number: 20030027306
    Abstract: The present invention provides amino alcohol dehydrogenases which catalyze a reaction to produce keto alcohols, ketones, aldehydes, keto acids from corresponding amino alcohols, amines, and amino acids in the presence of NAD+, and a reaction to produce amino alcohols, amines, amino acids from corresponding keto alcohols, ketones, aldehydes, and keto acids in the presence of NADH and ammonium ions. Also provided is a method for producing the enzymes and uses of the enzyme. The enzymes of the present invention can be obtained from microorganisms belonging to the genera Streptomyces, Pseudomonas, Burkholdenia, or Arthrobacter.
    Type: Application
    Filed: July 17, 2002
    Publication date: February 6, 2003
    Applicant: Daicel Chemical Industries, Ltd.
    Inventors: Nobuya Ito, Akinobu Matsuyama, Yoshinori Kobayashi
  • Patent number: 6514742
    Abstract: D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.
    Type: Grant
    Filed: July 27, 1999
    Date of Patent: February 4, 2003
    Assignee: Daicel Chemical Industries, Ltd.
    Inventors: Kazuya Mitsuhashi, Hiroaki Yamamoto, Akinobu Matsuyama, Shinji Tokuyama