Abstract: Some embodiments are directed to a process for forming products from animal waste (e.g., poultry litter). The products may include a controlled release fertilizer and renewable natural gas. The process may include providing a feedstock comprising greater than about 80 percent poultry waste and a moisture content of about 25 percent. The feedstock may be diluted to form a slurry having a moisture content of about 90%. The slurry may be mechanically refined to reduce the particle size distribution and the average particle size of the slurry. The slurry may be anaerobically digested to produce biogas and a digestate. The biogas may be converted to renewable natural gas, and the digestate may be converted to a controlled release fertilizer. Additionally, some embodiments are directed to methods of reducing nitrogen emission from soil amendments. Some embodiments are directed to reducing nitrogen emissions sufficient to produce emissions offset credits.

Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Abstract: The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

Abstract: Provided herein are methods of generating a population of enucleated erythroid cells.

Abstract: The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

Abstract: A playback page, which can include embedded media players and a user interface that functions as an Internet media station tuner, can be transmitted from a web server to a web browser running on a client computer. In response to receiving a message from the playback page indicating a user-selected media station, the web server transmits a message instructing the media station to transmit a tagged media stream to one media player (a Content Playback Instance), and transmits a browser file to an advertisement server. The browser file includes information used by the advertisement server to select targeted media content to be inserted into an untagged media stream delivered to another media player (an Ad Spot Playback Instance). The web server controls whether the Content Playback Instance presents a tagged media stream, or the Ad Spot Playback Instance presents targeted content, at any particular time.

Abstract: The present disclosure pertains to a mammalian cell culture genetically modified to express, and which expresses, a neublastin antibody polypeptide, or fragment thereof, in the culture, and to a neublastin antibody polypeptide, or fragment thereof, made by a mammalian cell culture genetically modified to express, and which expresses, the neublastin antibody polypeptide, or fragment thereof.

Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluyveromyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.

Abstract: Some aspects of the present disclosure provide methods, compositions and kits for identifying one or more reagents, such as a culture medium, that are effective for recombinant protein production.

Abstract: The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Abstract: A playback page, which can include embedded media players and a user interface that functions as an Internet media station tuner, can be transmitted from a web server to a web browser running on a client computer. In response to receiving a message from the playback page indicating a user-selected media station, the web server transmits a message instructing the media station to transmit a tagged media stream to one media player (a Content Playback Instance), and transmits a browser file to an advertisement server. The browser file includes information used by the advertisement server to select targeted media content to be inserted into an untagged media stream delivered to another media player (an Ad Spot Playback Instance). The web server controls whether the Content Playback Instance presents a tagged media stream, or the Ad Spot Playback Instance presents targeted content, at any particular time.

Abstract: The present invention pertains to a cell culture medium comprising hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine as media supplements which is shown to control viability, growth, and waste byproduct accumulation. The present invention further pertains to a method of producing a polypeptide of interest in a large scale cell culture containing hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine.

Abstract: Playback of a tagged audio stream at mobile communications device, or other processing device, can be controlled transparently to a user of the device by implementing multiple instances of a media player. A Spot Caching Instance of the media player can receive and cache an untagged content stream from an advertisement server. A Content Playback Instance of the media player can be used to play back and process a tagged content stream from a radio station to identify tags. When the Content Playback Instance detects a tag, it transfers control to an Ad Spot Playback Instance of the media player, which can play back part or all of the untagged content stream from cache. When playback of the untagged content stream is completed, the Ad Spot Playback Instance transfers control back to the Content Playback Instance.

Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.