Patents by Inventor Alan Sloma
Alan Sloma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140038235Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: ApplicationFiled: October 10, 2013Publication date: February 6, 2014Applicant: Novozymes Biopharma DK A/SInventors: Alan Sloma, Regine Behr, William Widner, Stephen Brown, Maria Tang, David Sternberg
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Publication number: 20120149067Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: ApplicationFiled: February 21, 2012Publication date: June 14, 2012Applicant: Novozymes, Inc.Inventors: Alan Sloma, Leslie Naggiar, Regine Behr, William Widner, Maria Tang, David Sternberg, Linda Sternberg, Stephen Brown
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Patent number: 8137951Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: GrantFiled: April 11, 2011Date of Patent: March 20, 2012Assignee: Novozymes, Inc.Inventors: Alan Sloma, Leslie Naggiar, legal representative, Regine Behr, William Widner, Maria Tang, David Sternberg, Linda Sternberg, legal representative, Stephen Brown
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Patent number: 8124376Abstract: The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: GrantFiled: February 10, 2010Date of Patent: February 28, 2012Assignee: Novozymes, Inc.Inventors: Maria Tang, Alan Sloma, Leslie Naggiar, legal representative, David Sternberg, Linda Sternberg, legal representative, Regine Behr
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Patent number: 8093036Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: GrantFiled: September 27, 2010Date of Patent: January 10, 2012Assignee: Novozymes, Inc.Inventors: Alan Sloma, Leslie Naggiar, legal representative, Regine Behr, William Widner, Maria Tang, David Sternberg, Linda Sternberg, legal representative, Stephen Brown
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Publication number: 20110189737Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: ApplicationFiled: April 11, 2011Publication date: August 4, 2011Applicant: Novozymes, Inc.Inventors: Alan Sloma, Regine Behr, William Widner, Maria Tang, David Sternberg, Stephen Brown, Leslie Naggiar, Linda Sternberg
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Publication number: 20110014662Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: ApplicationFiled: September 27, 2010Publication date: January 20, 2011Applicant: Novozymes, Inc.Inventors: Alan Sloma, Leslie Naggiar, Regine Behr, William Widner, Maria Tang, David Sternberg, Linda Sternberg, Stephen Brown
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Publication number: 20100330618Abstract: The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: ApplicationFiled: February 10, 2010Publication date: December 30, 2010Applicant: Novozymes, Inc.Inventors: Maria Tang, Alan Sloma, Leslie Naggiar, David Sternberg, Linda Sternberg, Regine Behr
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Patent number: 7811806Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: GrantFiled: December 20, 2002Date of Patent: October 12, 2010Assignee: Novozymes, Inc.Inventors: Alan Sloma, Regine Behr, William Widner, Maria Tang, David Sternberg, Stephen Brown
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Patent number: 7666625Abstract: The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: GrantFiled: December 9, 2008Date of Patent: February 23, 2010Assignee: Novozymes, Inc.Inventors: Maria Tang, Alan Sloma, David Sternberg, Linda Sternberg, legal representative, Regine Behr
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Publication number: 20090098606Abstract: The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: ApplicationFiled: December 9, 2008Publication date: April 16, 2009Applicant: Novozymes, Inc.Inventors: Maria Tang, Alan Sloma, David Stemberg, Linda Stemberg, Regine Behr
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Patent number: 7476516Abstract: The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: GrantFiled: July 25, 2003Date of Patent: January 13, 2009Assignee: Novozymes, Inc.Inventors: Maria Tang, Alan Sloma, David Sternberg, Regine Behr
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Patent number: 7179634Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.Type: GrantFiled: April 12, 2001Date of Patent: February 20, 2007Assignee: Novozymes, Inc.Inventors: William Widner, Alan Sloma, Michael D. Thomas
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Patent number: 7091000Abstract: Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.Type: GrantFiled: February 10, 2003Date of Patent: August 15, 2006Assignee: DSM Nutritional Products, Inc.Inventors: John B. Perkins, Alan Sloma, Janice G. Pero, Randolph T. Hatch, Theron Hermann, Thomas Erdenberger
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Publication number: 20050221446Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the hyaluronic acid, wherein the Bacillus cell comprises a nucleic acid construct comprising a triple promoter comprising a variant amyL promoter having a mutation corresponding to position 590 of SEQ ID NO: 1, a consensus promoter having the sequence TTGACA for the “?35” region and TATAAT for the “?10” region, and a cryIIIA promoter, in which each promoter sequence of the triple promoter is operably linked to one or more coding sequences involved in the biosynthesis of the hyaluronic acid; and (b) isolating the hyaluronic acid from the cultivation medium.Type: ApplicationFiled: March 31, 2005Publication date: October 6, 2005Applicant: Novozymes Biopolymer A/S.Inventors: William Widner, Alan Sloma, Michael Thomas, Maria Tang
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Publication number: 20050074844Abstract: The present invention relates to arrays for bacterial gene expression profiling in response to treatment with antimicrobial compounds. Bacterial nucleic acid molecules are used as the hybridizable elements in various applications, such as arrays for gene expression profiling and for determining a mode of action for an antimicrobial compound. The present invention also relates to nucleic acid sequences which can be developed as targets for drug screening.Type: ApplicationFiled: September 5, 2003Publication date: April 7, 2005Applicant: Novozymes Biotech, Inc.Inventors: Debbie Yaver, Alan Sloma
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Publication number: 20040096944Abstract: The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypx and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: ApplicationFiled: July 25, 2003Publication date: May 20, 2004Applicant: Novozymes Biotech, Inc.Inventors: Maria Tang, Alan Sloma, David Sternberg, Regine Behr
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Patent number: 6673571Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: September 19, 2001Date of Patent: January 6, 2004Assignee: Novozymes Biotech, Inc.Inventors: Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto, Lene Venke Kofod
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Publication number: 20030232406Abstract: Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.Type: ApplicationFiled: February 10, 2003Publication date: December 18, 2003Applicant: ROCHE VITAMINS, INC.Inventors: John B. Perkins, Alan Sloma, Janice G. Pero, Randolph T. Hatch, Theron Hermann, Thomas Erdenberger
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Publication number: 20030186380Abstract: The present invention relates to recombinant methods for producing a secreted polypeptide having L-asparaginase activity, comprising (a) cultivating under conditions conducive for production of the polypeptide a host cell comprising a nucleic acid construct comprising a first nucleic acid sequence encoding a secretory signal peptide operably linked to second nucleic acid sequence encoding the polypeptide having L-asparaginase activity, wherein the signal peptide directs the polypeptide into the cell's secretory pathway; and (b) recovering the secreted polypeptide. The present invention also relates to isolated polypeptides having L-asparaginase activity and nucleic acids thereof.Type: ApplicationFiled: April 1, 2003Publication date: October 2, 2003Applicant: Novozymes Biotech, Inc.Inventors: Michael D. Thomas, Alan Sloma