Patents by Inventor Alan Sloma
Alan Sloma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20030175902Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.Type: ApplicationFiled: December 20, 2002Publication date: September 18, 2003Applicant: Novozymes Biotech, Inc.Inventors: Alan Sloma, Regine Behr, William Widner, Maria Tang, David Sternberg, Stephen Brown
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Publication number: 20030170876Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.Type: ApplicationFiled: April 12, 2001Publication date: September 11, 2003Applicant: Novozymes Biotech, Inc.Inventors: William Widner, Alan Sloma, Michael D. Thomas
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Patent number: 6551813Abstract: Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.Type: GrantFiled: May 6, 1999Date of Patent: April 22, 2003Assignee: Roche Vitamins Inc.Inventors: John B. Perkins, Alan Sloma, Janice G. Pero, Randolph T. Hatch, Theron Hermann, Thomas Erdenberger
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Publication number: 20030044949Abstract: The present invention relates to isolated polypeptides having gamma-glutamyl transpeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: ApplicationFiled: March 27, 2002Publication date: March 6, 2003Applicant: Novozymes Biotech, Inc.Inventors: Regine Behr, Alan Sloma
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Publication number: 20020177210Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: ApplicationFiled: September 19, 2001Publication date: November 28, 2002Applicant: Novozymes Biotech, Inc.Inventors: Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto, Lene Venke Kofod
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Patent number: 6303360Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: April 5, 2000Date of Patent: October 16, 2001Assignees: Novozymes Biotech, Inc,, Novozymes A/S, Japan Tobacco, Inc.Inventors: Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto, Lene Venke Kofod
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Patent number: 6255076Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.Type: GrantFiled: February 26, 1999Date of Patent: July 3, 2001Assignee: Novozymes Biotech, Inc.Inventors: William Widner, Alan Sloma, Michael D. Thomas
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Patent number: 6184020Abstract: The present invention relates to isolated polypeptides having aminopeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: November 13, 1998Date of Patent: February 6, 2001Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/S, Japan Tobacco, Inc.Inventors: Alexander Blinkovsky, Tony S. Byun, Alan V. Klotz, Alan Sloma, Kimberly Brown, Maria Tang, Mikio Fujii, Chigusa Marumoto
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Patent number: 5958728Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a Bacillus cell, wherein the mutant (i) comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of a surfactin or isoform thereof under conditions conducive for the production of the polypeptide and (ii) the mutant produces less of the surfactin or isoform thereof than the Bacillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.Type: GrantFiled: November 18, 1997Date of Patent: September 28, 1999Assignee: Novo NordiskBiotech, Inc.Inventors: Alan Sloma, David Sternberg, Lee F. Adams, Stephen Brown
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Patent number: 5955310Abstract: The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.Type: GrantFiled: February 26, 1998Date of Patent: September 21, 1999Assignee: Novo Nordisk Biotech, Inc.Inventors: William Widner, Alan Sloma, Michael D. Thomas
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Patent number: 5925538Abstract: Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.Type: GrantFiled: August 24, 1998Date of Patent: July 20, 1999Assignee: Roche Vitamins Inc.Inventors: John B. Perkins, Alan Sloma, Janice G. Pero, Randolph T. Hatch, Theron Hermann, Thomas Erdenberger
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Patent number: 5891701Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having protease activity, in which the polypeptides are obtainable from an alkalophilic Bacillus species having enhanced stability towards bleaching agents of the peroxy type. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.Type: GrantFiled: June 12, 1997Date of Patent: April 6, 1999Assignee: Novo Nordisk BioTech Inc.Inventors: Alan Sloma, Lynne Christianson
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Patent number: 5874278Abstract: A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (RP-I) and proteolytically active residual protease II (RP-II) (mpr).Type: GrantFiled: April 15, 1997Date of Patent: February 23, 1999Assignee: OmniGene Inc.Inventors: Alan Sloma, Gerald A. Rufo, Jr., Cathy Faye Rudolph, Barbara J. Sullivan, Janice Pero
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Patent number: 5837528Abstract: Vectors and recombinant bacteria for overproducing riboflavin, in which nucleic acid overproducing riboflavin biosynthetic proteins is introduced in the chromosome of the host organism, e.g. at multiple sites and in multiple copies per site. A rib operon having at least five genes is used to make such recombinant bacteria.Type: GrantFiled: February 6, 1995Date of Patent: November 17, 1998Assignee: Hoffmann La Roche, Inc.Inventors: John B. Perkins, Alan Sloma, Janice G. Pero
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Patent number: 5620880Abstract: A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (RP-I) and proteolytically active residual protease II (RP-II).Type: GrantFiled: September 7, 1990Date of Patent: April 15, 1997Assignee: Omnigene, Inc.Inventors: Alan Sloma, Gerald A. Rufo, Jr., Cathy F. Rudolph, Barbara J. Sullivan, Janice Pero
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Patent number: 5589383Abstract: A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (bpr) and proteolytically active residual protease II (RP-II).Type: GrantFiled: August 12, 1994Date of Patent: December 31, 1996Assignee: Omnigene, Inc.Inventors: Alan Sloma, Gerald A. Rufo, Jr., Cathy F. Rudolph, Barbara J. Sullivan, Janice Pero
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Patent number: 5294542Abstract: A Bacillus cell containing a mutation in the residual protease III (rp-III) gene resulting in the inhibition of the production by the cell of proteolytically active RP-III.Type: GrantFiled: March 19, 1991Date of Patent: March 15, 1994Assignee: Omnigene, Inc.Inventors: Alan Sloma, Gerald A. Rufo, Jr., Janice Pero
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Patent number: 5171673Abstract: A vector for high-level expression of a heterologous gene in Bacillus, including a DNA sequence which encodes all or part of the structural gene and contains the promoter and ribosome binding site of a Bacillus coagulans amylase gene; within or immediately downstream of the structural gene is a site for insertion of heterologous DNA; when inserted, the heterologous DNA is in the same translational reading frame as the amylase gene; the heterologous DNA thus is under control of the B. coagulans amylase gene regulatory elements and expressed at high levels. A signal-encoding sequence may be inserted independent of, or along with, the heterologous DNA leading to authentic secretion of the heterologous protein.Type: GrantFiled: July 18, 1988Date of Patent: December 15, 1992Assignee: Biotechnica International, Inc.Inventors: Alan Sloma, Nancy M. Hannett, M. A. Stephens, Cathy F. Rudolph, Gerald A. Rufo, Jr., Janice Pero
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Patent number: 5017477Abstract: A bacterial cell transformed with a gene encoding a desired product and with an enhancing DNA sequence capable of enhancing the production of the desired product in the bacterial cell, the enhancing DNA sequence being further characterized in that it is capable of enhancing the production of an endogenous protease in a Gram-positive bacterial cell.Type: GrantFiled: October 20, 1986Date of Patent: May 21, 1991Assignee: BioTechnica International, Inc.Inventors: Alan Sloma, Rosalind C. Lee, Janice Pero
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Patent number: 4748233Abstract: A cloned human alpha-interferon Gx-1 gene, plasmids containing the human alpha-interferon Gx-1 gene, and microorganisms transformed by those plasmids are disclosed. Also disclosed is the polypeptide, alpha-interferon Gx-1.Type: GrantFiled: May 28, 1987Date of Patent: May 31, 1988Assignee: Bristol-Myers CompanyInventor: Alan Sloma