Abstract: Methods, compositions and kits for determining the likelihood of reaching the blastocyst stage for one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.

Abstract: A method of managing information system resources to provide a message flow having a consistent level of quality of service (QoS) within and across a plurality of interconnected middleware domains is described. The method includes receiving a QoS message from a first QoS manager expressing at least one QoS requirement, translating the at least one QoS requirement to at least one parameter specific to a messaging service that communicatively couples multiple middleware domains, creating a client connection between a first middleware domain and the messaging service for receiving the message flow, transmitting the QoS message to a second middleware domain, and creating a client connection between the messaging service and the second middleware domain for transmitting the message flow.

Abstract: Engineered human tissue constructs are provided that are suitable for use in making humanized animals for use in pharmaceutical development. Humanized animals having the constructs implanted in vivo are provided. Methods of making and using the tissue-engineered constructs and humanized animals are also provided.

Abstract: A method of managing resources of an information system relative to clients of the system. A quality of service (QoS) message is received from a client expressing QoS requirements as parameter values. A contract is established with the client for quality of service based on the one or more parameter values. One or more resources of the system are allocated to the client based on the contract. This method enables uniform resource management and polymorphic resource allocation across multiple middleware platforms.

Abstract: An interactive, handheld apparatus is provided. The apparatus includes a housing, a display screen on the housing, an electronic position location system, a stylus for selecting images on the display screen, a directional control pad on the housing and a memory device. The electronic position location system includes a processor that is capable of determining a location of a selected region of the display screen. The memory device includes computer code for an educational game and is operatively coupled to the processor.

Abstract: A method of managing information system resources to provide a message flow having a consistent level of quality of service (QoS) within and across a plurality of interconnected middleware domains is described. The method includes receiving a QoS message from a first QoS manager expressing at least one QoS requirement, translating the at least one QoS requirement to at least one parameter specific to a messaging service that communicatively couples multiple middleware domains, creating a client connection between a first middleware domain and the messaging service for receiving the message flow, transmitting the QoS message to a second middleware domain, and creating a client connection between the messaging service and the second middleware domain for transmitting the message flow.

Abstract: A method of managing information system resources to provide a message flow having a consistent level of quality of service (QoS) within and across a plurality of interconnected middleware domains is described. The method includes receiving a QoS message from a first QoS manager expressing at least one QoS requirement, translating the at least one QoS requirement to at least one parameter specific to a messaging service that communicatively couples multiple middleware domains, creating a client connection between a first middleware domain and the messaging service for receiving the message flow, transmitting the QoS message to a second middleware domain, and creating a client connection between the messaging service and the second middleware domain for transmitting the message flow.

Abstract: Methods and compositions for tracking or monitoring uptake of siRNA by mammalian cells are provided. The methods and compositions may be used to monitoring the silencing activity of the internalized siRNA. The compositions contain an siRNA, an optically or magnetically detectable nanoparticle such as a quantum dot and, optionally, a transfection reagent. Cells are contacted with an siRNA and an optically or magnetically detectable nanoparticle, optionally in the presence of a transfection reagent. Detection of internalized nanoparticles is indicative of siRNA uptake. The invention allows analysis, identification, processing, etc., of cells that have efficiently taken up siRNA. In one embodiment, cells are sorted into at least two populations based on the amount of siRNA taken up.

Abstract: An interactive, handheld apparatus is provided. The apparatus includes a housing, a display screen on the housing, an electronic position location system, a stylus for selecting images on the display screen, a directional control pad on the housing and a memory device. The electronic position location system includes a processor that is capable of determining a location of a selected region of the display screen. The memory device includes computer code for an educational game and is operatively coupled to the processor.

Abstract: A method of managing information system resources to provide a message flow having a consistent level of quality of service (QoS) within and across a plurality of interconnected middleware domains is described. The method includes receiving a QoS message from a first QoS manager expressing at least one QoS requirement, translating the at least one QoS requirement to at least one parameter specific to a messaging service that communicatively couples multiple middleware domains, creating a client connection between a first middleware domain and the messaging service for receiving the message flow, transmitting the QoS message to a second middleware domain, and creating a client connection between the messaging service and the second middleware domain for transmitting the message flow.

Abstract: An interactive, handheld apparatus is provided. The apparatus includes a housing, a display screen on the housing, an electronic position location system, a stylus for selecting images on the display screen, a directional control pad on the housing and a memory device. The electronic position location system includes a processor that is capable of determining a location of a selected region of the display screen. The memory device includes computer code for an educational game and is operatively coupled to the processor. A light is associated with the stylus. The light is illuminated to indicate that the stylus is available for use during operation of the educational game.

Abstract: A method for administering object-based multi-level security in a service oriented architecture includes: (a) defining a plurality of multi-level security attributes for each of selected respective life-cycle states of a plurality of life-cycle states of a service object; (b) receiving a request from a requestor for the service object; (c) determining permitted actions for the service object based upon at least one selected multi-level security attribute of the plurality of multi-level security attributes, and based upon at least one life-cycle state of the plurality of life-cycle states of the service object; and (d) generating a quality of service security contract based upon the determination of permitted actions.

Abstract: Method and system of building a reasoning model using relational databases is provided. The method includes identifying data objects in relational databases; determining dependency relationships between the data objects; translating the data objects into nodes of a Bayesian network; and automatically translating the dependency relationships into a graphical structure of a Bayesian network. The system includes at least one server for storing data of a system having numerous interconnected parts; monitoring agents for monitoring the data of the numerous interconnected parts stored in the system; an events log for storing any event observed by the monitoring agents; and relational databases for storing data objects, the data objects correspond to the data of the numerous interconnected parts.

Abstract: An interactive, handheld apparatus is provided. The apparatus includes a housing, a display screen on the housing, an electronic position location system, a stylus for selecting images on the display screen, a directional control pad on the housing and a memory device. The electronic position location system includes a processor that is capable of determining a location of a selected region of the display screen. The memory device includes computer code for an educational game and is operatively coupled to the processor.

Abstract: A method of managing resources of an information system relative to clients of the system. A quality of service (QoS) message is received from a client expressing QoS requirements as parameter values. A contract is established with the client for quality of service based on the one or more parameter values. One or more resources of the system are allocated to the client based on the contract. This method enables uniform resource management and polymorphic resource allocation across multiple middleware platforms.

Abstract: The present invention provides purified disease detection and treatment molecule polynucleotides (mddt). Also encompassed are the polypeptides (MDDT) encoded by mddt. The invention also provides for the use of mddt, or complements, oligonucleotides, or fragments thereof in diagnostic assays. The invention further provides for vectors and host cells containing mddt for the expression of MDDT. The invention additionally provides for the use of isolated and purified MDDT to induce anitbodies and to screen libraries of compounds and the use of anti-MDDT antibodies in diagnostic assays. Also provided are microarrays containing mddt and methods of use.

Abstract: An interactive, handheld apparatus is provided. The apparatus includes a housing, a display screen on the housing, an electronic position location system, a stylus for selecting images on the display screen, a directional control pad on the housing and a memory device. The electronic position location system includes a processor that is capable of determining a location of a selected region of the display screen. The memory device includes computer code for an educational game and is operatively coupled to the processor. A light is associated with the stylus. The light is illuminated to indicate that the stylus is available for use during operation of the educational game.

Abstract: The present invention provides novel JNK activating phosphatase polypeptides and nucleic acid molecules encoding the same. The invention also provides vectors, host cells, antibodies and methods for producing JNK activating phosphatase polypeptides. Also provided for are methods for the diagnosis and treatment of diseases associated with JNK activating phosphatase polypeptides.

Abstract: An interactive, handheld apparatus is provided. The apparatus includes a housing, a display screen on the housing, an electronic position location system, a stylus for selecting images on the display screen, a directional control pad on the housing and a memory device. The electronic position location system includes a processor that is capable of determining a location of a selected region of the display screen. The memory device includes computer code for an educational game and is operatively coupled to the processor.

Abstract: The present invention provides purified secretory polynucleotides (sptm). Also encompassed are the polypeptides (SPTM) encoded by sptm. The invention also provides for the use of sptm, or complements, oligonucleotides, or fragments thereof in diagnostic assays. The invention further provides for vectors and host cells containing sptm for the expression of SPTM. The invention additionally provides for the use of isolated and purified SPTM to induce antibodies and to screen libraries of compounds and the use of anti-SPTM antibodies in diagnostic assays. Also provided are microarrays containing sptm and methods of use.