Patents by Inventor Amit Choudhary

Amit Choudhary has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11983746
    Abstract: Systems, apparatuses, and methods for providing a more effective customer service support system. In some embodiments, the systems, apparatuses, and methods include a user interface (UI) and process flow for implementing a conversation-based customer service support system. The user interface and associated processing enable a user/customer to quickly and accurately select an intent, goal, or purpose of their customer service request. In some embodiments, this is achieved by a set of user interface displays or screens and an underlying logic that provide a user with multiple ways of selecting, identifying, or describing the intent, goal, or purpose of their request for service or assistance.
    Type: Grant
    Filed: January 20, 2021
    Date of Patent: May 14, 2024
    Assignee: Helpshift, Inc.
    Inventors: Amit Choudhary, Swaroop Chitlur Haridas, Christian Leipski, Yashkumar Gandhi, Shyam Hirachand Shinde
  • Patent number: 11965159
    Abstract: Compositions and methods for conditionally regulating activities of CRISPR-Cas systems. In some embodiments, the methods comprise providing an inactive guide RNA comprising a regulatory domain bound by a lock nucleic acid different from the guide RNA; and displacing the lock nuclei acid from the regulatory domain by a trigger nucleic acid, thereby activating the guide RNA, wherein the activated guide RNA forms a complex with a Cas enzyme.
    Type: Grant
    Filed: January 29, 2020
    Date of Patent: April 23, 2024
    Assignees: The Broad Institute, Inc., The Brigham and Women's Hospital, Inc., The Regents of the University of California
    Inventors: Amit Choudhary, Kurt Cox, Hari Subramanian, Elisa Franco
  • Publication number: 20240115557
    Abstract: The need to control the activity and fidelity of CRISPR-associated nucleases has resulted in the demand for inhibitory anti-CRISPR molecules. Current small-molecule inhibitor discovery platforms are not generalizable to multiple nuclease classes, only target the initial step in the catalytic activity, and require high concentration of nuclease, resulting in inhibitors with suboptimal attributes, including poor potency. Herein, Applicants report a high-throughput discovery pipeline consisting of a FRET-based assay that is generalizable to contemporary and emerging nucleases, operates at low nuclease concentration, and targets all catalytic steps. Applicants applied this pipeline to identify BRD7586, a cell-permeable small-molecule inhibitor of SpCas9, that is 2-fold more potent than current inhibitors. Furthermore, unlike the reported inhibitors, BRD7586 enhanced SpCas9 specificity and its activity was independent of the genomic loci, DNA repair pathway, or mode of nuclease delivery.
    Type: Application
    Filed: July 28, 2023
    Publication date: April 11, 2024
    Inventors: Amit Choudhary, Donghyun Lim, Qingxuan Zhou, Kurt Cox, Benjamin Law, Miseon Lee, Praveen Kokkonda, Sreekanth Vedagopuram
  • Publication number: 20240024490
    Abstract: The present disclosure relates to bifunctional chemical conjugation molecules, which find utility as modifiers of target substrates. The present disclosure includes multifunctional compounds comprising an enzyme binding moiety, a chemical linker moiety, and a target binding moiety, which may further include an electrophilic reactive group. Molecules according to the present invention find use making substrate modifications such as post-translational modifications to proteins that are not the natural substrate of the enzyme. Diseases or disorders may be treated or prevented with molecules of the present disclosure.
    Type: Application
    Filed: April 8, 2022
    Publication date: January 25, 2024
    Inventors: Amit Choudhary, Veronika Shoba, Arghya Deb, Tuan Nguyen, Sophia Lai, Dhanushka Munkanatta Godage, Praveen Tiwari, Ashley Modell, Sachini Siriwardena
  • Patent number: 11787795
    Abstract: Compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.
    Type: Grant
    Filed: October 31, 2017
    Date of Patent: October 17, 2023
    Assignees: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, THE BRIGHAM AND WOMEN'S HOSPITAL, INC., THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Amit Choudhary, Peng Wu, Basudeb Maji, Elisa Franco, Hari K. K. Subramanian
  • Patent number: 11760984
    Abstract: The embodiments disclosed herein utilize fluorescence polarization based preliminary screen to identify a putative set of Cas inhibitors from an initial set of candidate inhibitors. The primary screening assay is followed by secondary screening assay to validate the putative set of inhibitors selected by the preliminary screen. In some embodiments, the present disclosure includes compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: September 19, 2023
    Inventors: Amit Choudhary, Kurt Cox, Basudeb Maji, Peng Wu, Hari Subramanian, Elisa Franco
  • Publication number: 20230192904
    Abstract: The present disclosure relates to chimeric small molecules, which find utility as modifiers of target substrates according to the formula A-L1-E-B or A-L1-E-L2-B, wherein A is a kinase binding moiety; B is a target binding moiety; L1 and L2 are each a linker; and E is an electrophilic reactive group. Molecules according to the present invention find use making substrate modifications such as post-translational modifications to targets that are not the natural substrate of the kinase; accordingly, diseases or disorders may be treated or prevented with molecules of the present disclosure.
    Type: Application
    Filed: November 18, 2022
    Publication date: June 22, 2023
    Inventors: Amit Choudhary, Vika Anokhina
  • Publication number: 20230151342
    Abstract: The disclosure includes compositions comprising synthetic zinc finger degrons, and their use with non-naturally occurring or engineered programmable nucleases. Compositions specifically targeting the engineered programmable nucleases for control of gene editing outcomes, and compositions, systems and method of use are further detailed.
    Type: Application
    Filed: February 26, 2021
    Publication date: May 18, 2023
    Inventors: Amit CHOUDHARY, Donghyun LIM, Sreekanth VEDAGOPURAM, Benjamin EBERT, Max JAN
  • Publication number: 20230117831
    Abstract: The present disclosure relates to multifunctional chemical conjugation molecules, which find utility as modifiers of target substrates. The present disclosure includes multifunctional compounds comprising a localizing moiety, a chemical linker moiety, an activator moiety, a first orienting adaptor interconnecting the chemical linker moiety on one end to the activator moiety, and optionally a second orienting adaptor interconnecting the chemical linker molecule on a different end to the localizing moiety. Molecules according to the present invention find use making post-translational modifications to macromolecules that are not the natural substrate of the activator moiety. Diseases or disorders may be treated or prevented with molecules of the present disclosure.
    Type: Application
    Filed: January 8, 2021
    Publication date: April 20, 2023
    Inventors: Amit CHOUDHARY, Sophia LAI, Veronika SHOBA, Praveen KOKKONDA, Dhanushka MUNKANATTA GODAGE, Sachini SIRIWARDENA
  • Publication number: 20220175848
    Abstract: An engineered cell is provided that may include one or more polynucleotides inserted into a sequence of a secretory protein of the cell, which may be a ?-cell.
    Type: Application
    Filed: April 1, 2020
    Publication date: June 9, 2022
    Inventors: Amit CHOUDHARY, Sreekanth VEDAGOPURAM, Donghyun LIM
  • Publication number: 20220162649
    Abstract: The present inventions generally relate to site-specific delivery of nucleic acid modifiers and includes novel DNA-binding proteins and effectors that can be rapidly programmed to make site-specific DNA modifications. The present inventions also provide synthetic all-in-one genome editor (SAGE) systems comprising designer DNA sequence readers and a set of small molecules that induce double-strand breaks, enhance cellular permeability, inhibit NHEJ and activate HDR, as well as methods of using and delivering such systems.
    Type: Application
    Filed: April 1, 2020
    Publication date: May 26, 2022
    Inventors: Amit CHOUDHARY, Donghyun LIM, Praveen KOKKONDA, Sophia LAI, Miseon LEE
  • Publication number: 20220154222
    Abstract: The present inventions generally relate to site-specific delivery of nucleic acid modifiers and includes novel DNA-binding proteins and effectors that can be rapidly programmed to make site-specific DNA modifications. The present inventions also provide a synthetic all-in-one genome editor (SAGE) systems comprising designer DNA sequence readers and a set of small molecules that induce double-strand breaks, enhance cellular permeability, inhibit NHEJ and activate HDR, as well as methods of using and delivering such systems.
    Type: Application
    Filed: March 13, 2020
    Publication date: May 19, 2022
    Inventors: Amit CHOUDHARY, Veronika SHOBA, Sreekanth VEDAGOPURAM
  • Publication number: 20220098620
    Abstract: The present inventions generally relate to site-specific delivery of nucleic acid modifiers and includes novel DNA-binding proteins and effectors that can be rapidly programmed to make site-specific DNA modifications. The present inventions also provide synthetic all-in-one genome editor (SAGE) systems comprising designer DNA sequence readers and a set of small molecules that induce double-strand breaks, enhance cellular permeability, inhibit NHEJ and activate HDR, as well as methods of using and delivering such systems.
    Type: Application
    Filed: July 9, 2021
    Publication date: March 31, 2022
    Inventors: Amit CHOUDHARY, Donghyun LIM, Praveen KOKKONDA, Sophia LAI
  • Publication number: 20220010282
    Abstract: 3D cell cultures and devices for 3D cell culture, and methods of use thereof are provided. In some embodiments, the 3D cell culture comprise pancreatic ? cells and can be generated in multi-well plates, allowing for high throughput assays on the cell culture.
    Type: Application
    Filed: June 16, 2021
    Publication date: January 13, 2022
    Applicants: THE BROAD INSTITUTE, INC., THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
    Inventors: Jeffrey Karp, Amit Choudhary, Kisuk Yang, Miseon Lee, Peter Jones
  • Publication number: 20210355522
    Abstract: Compositions and methods are provided for the inhibition of the function of RNA guided endonucleases, including the identification and use of such inhibitors. Methods of identifying inhibitor compounds of RNA guided nucleases, assays for detecting nuclease activity and compounds for use therein are also provided.
    Type: Application
    Filed: August 20, 2019
    Publication date: November 18, 2021
    Inventors: Amit CHOUDHARY, Kurt COX
  • Publication number: 20210324357
    Abstract: The disclosure includes non-naturally occurring or engineered CRISPR Cas variant proteins comprising one or more functional domains and degrader compositions specifically targeting the CRISPR Cas variant proteins; compositions, systems and methods of using the CRISPR Cas variant proteins comprising one or more functional domains and degrader compounds for spatio-temporal control are also provided.
    Type: Application
    Filed: August 20, 2019
    Publication date: October 21, 2021
    Inventors: Amit CHOUDHARY, Qingxuan ZHOU, Praveen KOKKONDA
  • Publication number: 20210317429
    Abstract: The disclosure includes non-naturally occurring or engineered DNA- or RNA-guided nuclease systems, comprising CRISPR enzymes associated with at least one destabilization domain (DD) and photocaged stabilization ligands with at least one photocage molecule, along with compositions, systems and complexes involving such systems, nucleic acid molecules and vectors encoding the same, delivery systems involving the same, uses thereof.
    Type: Application
    Filed: August 20, 2019
    Publication date: October 14, 2021
    Inventors: Amit CHOUDHARY, Debasish MANNA, Basudeb MAJI, Soumyashree Ashok GANGOPADHYAY
  • Publication number: 20210224818
    Abstract: Systems, apparatuses, and methods for providing a more effective customer service support system. In some embodiments, the systems, apparatuses, and methods include a user interface (UI) and process flow for implementing a conversation-based customer service support system. The user interface and associated processing enable a user/customer to quickly and accurately select an intent, goal, or purpose of their customer service request. In some embodiments, this is achieved by a set of user interface displays or screens and an underlying logic that provide a user with multiple ways of selecting, identifying, or describing the intent, goal, or purpose of their request for service or assistance.
    Type: Application
    Filed: January 20, 2021
    Publication date: July 22, 2021
    Inventors: Amit Choudhary, Swaroop Chitlur Haridas, Christian Leipski, Yashkumar Gandhi, Shyam Hirachand Shinde
  • Publication number: 20210222164
    Abstract: The disclosure includes non-naturally occurring or engineered DNA- or RNA-guided nuclease systems, comprising guide-binding adaptors each associated with at least one destabilization domain (DD), along with compositions, systems and complexes involving such systems, nucleic acid molecules and vectors encoding the same, delivery systems involving the same, uses therefor.
    Type: Application
    Filed: June 29, 2017
    Publication date: July 22, 2021
    Inventors: Amit CHOUDHARY, Basudeb MAJI, Bernd ZETSCHE, Feng ZHANG
  • Publication number: 20210224306
    Abstract: Systems, apparatuses, and methods for providing a more effective customer service support system. A trained model may be created or generated by a process that includes acquiring a corpus of documents (such as customer generated messages), generating keyphrases from each document in the corpus of documents, generating word embeddings from each document in the corpus of documents, performing a clustering operation on the word embeddings, assigning each document in the corpus to a duster and labeling each duster, and training a machine learning or text classification model using the most relevant documents in a cluster and the label assigned to that cluster. The methods include a processing flow that provides multiple ways for a customer to navigate and select their desired intent or purpose in seeking customer assistance. As part of the processing flow, the trained machine learning model and intent tree data are provided to a customer seeking assistance.
    Type: Application
    Filed: January 20, 2021
    Publication date: July 22, 2021
    Inventors: Amit Choudhary, Swaroop Chitlur Haridas, Christian Leipski, Yashkumar Gandhi, Shyam Hirachand Shinde