Abstract: An enzymatic process is described for the production of chemicals from carbon sources. In particular, according to one aspect, a process for the production of a target organic compound from a carbon source by a cell-free enzyme system is disclosed.

Abstract: The present invention relates to enzymes and processes. In particular, there is described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme and variants thereof.

Abstract: The invention concerns a process for the recovery of an organic moiecule from the overhead vapor phase of aqueous media by adsorbing onto an adsorbent, wherein no additional thermal energy for said vaporization is provided, and wherein the organic molecule is recovered afterwards by desorbing from the adsorber.

Abstract: The invention relates to a method for depolymerizing materials containing carbohydrates comprising the following steps: (a) treating a material containing carbohydrates with an inorganic catalyst in order to release defined monomeric or oligomeric building blocks from the material containing the carbohydrates; and (b) separating the defined monomeric or oligomeric building blocks produced in step (a) from the rest of the carbohydrate-containing material. Preferably, the inorganic catalyst used in step (a) comprises tectosilicates, phyilosilicates or hydrotalcites and more preferably zeolites or bentonites. The carbohydrate-containing material further comprises preferably LCB and the defined monomeric or oligomeric building blocks are preferably glucoses, xyloses, arabinoses and oligomers thereof. Other aspects of the invention refer to the use of solution promoters in combination with the inorganic catalyst.

Abstract: The invention relates to a synthetic bifunctional non-antibody compound comprising one or more effector moieties and one or more binder moieties, wherein the effector moieties are operably linked to the binder moieties via a linker, the effector moieties are ligands to at least one pathogen pattern recognition receptor (PRR) and the binder moieties bind to a marker of a tumor cell.

Abstract: The invention relates to microstructures and the use thereof for the directed evolution of biomolecules.

Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.

Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.

Abstract: A method of fluorescence measurement on analytes, marked with different fluorescent markers, which have spectrally different fluorescence emissions, in a sample is described, having the following steps: illumination of the sample in the measurement volume using a laser to excite the fluorescence emission of the at least two fluorescent markers, the illumination of the measurement volume being performed with a maximum of one single laser line, having an excitation intensity sufficiently high that the fluorescent markers are jointly excited through 2-photon absorptions, detection of the fluorescence emission using at least two detector devices which are designed for light detection in different spectral ranges in accordance with the spectral fluorescence properties of the fluorescent markers, and performance of a cross-correlation and/or coincidence analysis of detector signals of the detector devices. A measurement device for performing the method is also described.

Abstract: The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.

Abstract: The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b).

Abstract: A process for generating sequence-specific proteases by screening-based directed evolution is disclosed. The use of the process provides proteases recognizing and cleaving user-definable amino-acid sequences with high sequence-specificity. Proteases obtainable by the process can be used in a variety of medical, diagnostic and industrial applications.

Abstract: The present invention provides engineered enzymes generated from protein scaffolds combined with Specificity Determining Regions, the production thereof and the use of said engineered enzymes for research, nutritional care, personal care and industrial purposes.

Abstract: The present invention relates to enzymes and processes. In particular, there is described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme and variants thereof.

Abstract: A method for analyzing properties of a sample by measuring fluorescence parameters in multiple foci, comprises the steps of splitting a collimated primary laser beam with a splitting device into at least two collimated secondary laser beams and deflecting the secondary laser beams such that they propagate at different propagation angles with respect to an optical axis of a focussing optic, focussing the secondary laser beams with the focussing optic into at least two volume elements in the sample, detecting light emitted from the volume elements with a detecting device, and evaluating the detected light for obtaining the properties to be analyzed. Furthermore, a device for implementing this method is described. The invention is particularly suited for high-throughput screening applications.

Abstract: The present invention provides variants of serine proteases of the S1 class with altered sensitivity to one or more activity-modulating substances. A method for the generation of such proteases is disclosed, comprising the provision of a protease library encoding polynucleotide sequences, expression of the enzymes, screening of the library in the presence of one or several activity-modulating substances, selection of variants with altered sensitivity to one or several activity-modulating substances and isolation of those polynucleotide sequences that encode for the selected variants.

Abstract: The invention relates to microstructures and the use thereof for the directed evolution of biomolecules.

Abstract: The present invention provides method for the treatment of a disease by applying a medicament comprising a protease with a defined specificity is capable to hydrolyze specific peptide bonds within a target substrate related to such disease. The proteases with such a defined specificity can further be used for related therapeutic or diagnostic purposes.

Abstract: The present invention relates to a method for the production of nucleic acids consisting of stochastically combined parts of source nucleic acids as well as to a kit for carrying out said method.

Abstract: The invention concerns an autofluorescent fusion protein suitable for use as a protease substrate, a nucleic acid sequence which encodes this fusion protein, and a method of using the fusion protein and/or the nucleic acid sequence in a dual color, confocal fluorometric assay for the detection and quantification of proteolytic activity or proteolytic inhibitory activity in samples or cells.