Abstract: A method of additive manufacturing is disclosed, comprising using a powder comprising a first particulate component (1) with a first mean particle diameter, and a second particulate component (2) with a second mean particle diameter. The first mean particle diameter is at least twice the second mean particle diameter. The particles (2) of the second component are bonded to the particles (1) of the first component, and the first and second components comprise different materials. The powder is deposited.

Abstract: Methods of preparing double-stranded nucleic acids with single-stranded overhangs for amplification and sequencing are disclosed. Contacting a blunt-ended double-stranded nucleic acid molecules with Taq results in non-templated directed addition of a single nucleotide to the 3? ends of the nucleic acid with A added most frequently followed by G followed by C and T. G tailing is sufficiently frequent that the efficiency of ligation of nucleic acid molecules to adapters can be significantly increased by including adapters tailed with T and C. The ligation efficiency can be increased even further with blunted-ended adapters to ligate to blunt-ended nucleic acid molecules that failed to undergo tailing.

Abstract: In an aspect, a method for evaluating the partitioning of nucleic acid molecules in a sample of polynucleotides based on epigenetic state, comprising: (a) adding a set of epigenetic-control nucleic acid molecules to the nucleic acid molecules in the sample of polynucleotides, whereby producing a spiked-in sample; (b) partitioning nucleic acid molecules of the spiked-in sample into plurality of partitioned sets; (c) enriching a subset of molecules from the plurality of partitioned sets to generate enriched molecules, wherein the enriched molecules comprises a group of epigenetic-control nucleic acid molecules and a group of nucleic acid molecules from the sample of polynucleotides; (d) sequencing the enriched molecules to produce sequencing reads; (e) analyzing the sequencing reads to generate one or more epigenetic partition scores of the epigenetic-control nucleic acid molecules; and (f) comparing the one or more epigenetic partition scores with one or more epigenetic partition cut-offs.

Abstract: This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.

Abstract: The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis.

Abstract: Provided herein are methods that are useful in determining the cellular origin of cell-free nucleic acid (cfNA) fragments from cfNA samples, such as liquid biopsy samples. The methods disclosed herein typically improve the specificity and/or sensitivity of assays for detecting diseased cell nucleic acids (e.g., cancer cell DNA) in cfNA samples by identifying variant alleles produced by non-target cells, such as hematopoietic stem cells, in certain embodiments. Yet other aspects include related systems and computer readable media, among numerous other applications.

Abstract: Disclosed herein are methods, compositions, and devices for use in early detection of cancer. The methods include sequencing a panel of regions in cell-free nucleic acid molecules and detecting one or more tumor markers that are indicative of a cancer.

Abstract: A prosthetic socket fitment system for fitting a prosthesis to a patient can include at least one transceiver assembly in contact with an external frame coupled to a patient, and a processing module in electrical communication with the at least one transceiver assembly. The at least one transceiver assembly can be aligned to the bone of the patient and configured to generate a signal directed to the bone of the patient and receive a reflected signal from the bone of the patient.

Abstract: An external catalyst cooler arrangement for an FCC regenerator improves the operation of the catalyst cooler by the use of a heat removal unit design utilizing a central supply tube and central heat removal conduit surrounded by external heat removal tubes connected directly to the central heat removal conduit.

Abstract: Methods of preparing double-stranded nucleic acids with single-stranded overhangs for amplification and sequencing are disclosed. Contacting a blunt-ended double-stranded nucleic acid molecules with Taq results in non-templated directed addition of a single nucleotide to the 3? ends of the nucleic acid with A added most frequently followed by G followed by C and T. G tailing is sufficiently frequent that the efficiency of ligation of nucleic acid molecules to adapters can be significantly increased by including adapters tailed with T and C. The ligation efficiency can be increased even further with blunted-ended adapters to ligate to blunt-ended nucleic acid molecules that failed to undergo tailing.

Abstract: The present disclosure is directed to a bicycle parking structure comprising a plurality of bicycle hangers supported by a crossbar assembly. Each bicycle hanger is configured to support a bicycle in a substantially vertical position. The bicycle hangers are spaced apart from one another along the length of the crossbar assembly. Each of the bicycle hangers may be secured to the crossbar assembly so that the bicycle hanger is fixed at a desired position or unsecured from the crossbar assembly so that the position of the bicycle hanger along the length of the crossbar assembly may be adjusted. In this manner, the distance between adjacent bicycles need not be fixed and the bicycle parking structure can be adapted for a particular space. The bicycle parking structure is designed to provide for a space-efficient, high-volume parking of bicycles.

Abstract: This invention discloses reagents and methods for increasing specificity and efficiency of RNA-guided genome editing.

Abstract: Imidamide (amidine) analogs that can inhibit the activity of sphingosine kinase 1 and sphingosine kinase 2 (SphK1 & SphK2) are provided. The compounds can prevent angiogenesis in tumors.

Abstract: The present disclosure is directed to a bicycle parking structure comprising a plurality of bicycle hangers supported by a crossbar assembly. Each bicycle hanger is configured to support a bicycle in a substantially vertical position. The bicycle hangers are spaced apart from one another along the length of the crossbar assembly. Each of the bicycle hangers may be secured to the crossbar assembly so that the bicycle hanger is fixed at a desired position or unsecured from the crossbar assembly so that the position of the bicycle hanger along the length of the crossbar assembly may be adjusted. In this manner, the distance between adjacent bicycles need not be fixed and the bicycle parking structure can be adapted for a particular space. The bicycle parking structure is designed to provide for a space-efficient, high-volume parking of bicycles.

Abstract: A virtual edge router network for providing managed services to distributed remote office locations can include routing components that are capable of being autonomously deployed at the network edge, as well as remotely managed, thereby obviating the need for on-site technical support in remote offices of the a small and medium business (SMB) client. Autonomous deployment and remote management is achieved through abstraction of the control and management planes from the data plane. Virtual edge routers may include virtual forwarding units and virtual remote agents instantiated on host devices in each remote office location, as well as a virtual network controller instantiated on a host device in a head-office location. A data plane of the virtual edge router communicatively couples the virtual forwarding units to one another, while a control plane communicatively couples the virtual network controller to each virtual data forwarding unit.

Abstract: This invention relates to reagents and methods for increasing specificity and efficiency of genome editing by CRISPR associated (Cas) protein systems, more particularly by Cas:guide RNA complexes, by blocking off-target nucleic acids from cleavage by Cas:guide RNA complexes.

Abstract: The invention relates to inhibitors of Sphingosine Kinase enzymatic activity, and methods of treating diseases and disorders by administering inhibitors of Sphingosine Kinase enzymatic activity.

Abstract: Imidamide (amidine) analogs that can inhibit the activity of sphingosine kinase 1 and sphingosine kinase 2 (SphK1 & SphK2) are provided. The compounds can prevent angiogenesis in tumors.

Abstract: The present invention relates to guide RNAs comprising adaptor segments having one or more modifications, and their use in homologous recombination by CRISPR:Cas systems. The modified adaptor segments are resistant to degradation by RNaseH. The present invention also relates to a dual guide RNA strategy in which a first guide RNA directs a Cas enzyme to make a double-strand break at a first target sequence, and a second guide RNA comprises an adaptor segment attached to a donor polynucleotide, and binds a second target sequence that is offset from the first target sequence.