Abstract: In one embodiment, a data processing method comprises obtaining one or more first photoplethysmography (PPG) signals based on one or more first light sources that are configured to emit light having a first light wavelength corresponding to a green light wavelength; obtaining one or more second PPG signals based on one or more second light sources that are configured to emit light having a second light wavelength corresponding to a red light wavelength, one or more of the first light sources and one or more of the second light sources being co-located; generating an estimated heart rate value based on one or more of the first PPG signals and the second PPG signals; and causing the estimated heart rate value to be displayed via a user interface on a client device.

Abstract: An activity monitoring device, methods and computer readable media are provided. The activity monitoring device includes a housing configured for attachment to a body part of a user and a display screen attached to the housing. Further included is a first sensor disposed in the housing for capturing motion of the activity monitoring device when attached to the body part of the user and a second sensor disposed in the housing for sampling a heart rate of the user. Memory is disposed in the housing for storing the motion captured by the first sensor and the heart rate sampled by the second sensor. A processor is disposed in the housing and is configured to determine a physical state of the user during a period of time. For motion that is below a threshold the processor identifies the physical state to be a sedentary state and for motion that is at or above the threshold the processor identifies the physical state to be an active state.

Abstract: The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black® RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is ˜200-fold over background, which is considerably better than other donor-quencher pairs (STNRs ˜10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.

Abstract: The disclosure provides BMDs that have multiple device modes depending on operational conditions of the devices, e.g., motion intensity, device placement, and/or activity type. The device modes are associated with various data processing algorithms. In some embodiments, the BMD is implemented as a wrist-worn or arm-worn device. In some embodiments, methods for tracking physiological metrics using the BMDs are provided. In some embodiments, the process or the BMD applies a time domain analysis on data provided by a sensor of the BMD for a first activity, and applies a frequency domain analysis on the data for a second activity, which contributes to improved accuracy and speed of biometric data.

Abstract: In one embodiment, a data processing method comprises obtaining one or more first photoplethysmography (PPG) signals based on one or more first light sources that are configured to emit light having a first light wavelength corresponding to a green light wavelength; obtaining one or more second PPG signals based on one or more second light sources that are configured to emit light having a second light wavelength corresponding to a red light wavelength, one or more of the first light sources and one or more of the second light sources being co-located; generating an estimated heart rate value based on one or more of the first PPG signals and the second PPG signals; and causing the estimated heart rate value to be displayed via a user interface on a client device.

Abstract: Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.

Abstract: Systems and methods for continuous flow polymerase chain reaction (PCR) are provided. The system comprises an injector, a mixer, a coalescer, a droplet generator, a detector, a digital PCR system, and a controller. The injector takes in a sample, partitions the sample into sample aliquots with the help of an immiscible oil phase, dispenses waste, and sends the sample aliquot to the mixer. The mixer mixes the sample aliquot with a PCR master mix and diluting water, dispenses waste, and sends the sample mixture (separated by an immiscible oil) to the coalescer. The coalescer coalesces the sample mixture with primers dispensed from a cassette, dispenses waste, and sends the reaction mixture (separated by an immiscible oil) to the droplet generator. The droplet generator converts the sample mixture into an emulsion where aqueous droplets of the reaction mixture are maintained inside of an immiscible oil phase and dispenses droplets to the digital PCR system.

Abstract: Biometric monitoring devices, including various technologies that may be implemented in such devices, are discussed herein. Additionally, techniques for utilizing gyroscopes in biometric monitoring devices are provided. Such techniques may, in some implementations, involve obtaining swimming metrics regarding stroke cycle count, lap count, and stroke type. Such techniques may also, in some implementations, involve obtaining performance metrics for bicycling activities.

Abstract: An aspect of the disclosure pertains to a blood pressure measurement device and methods of controlling an inflation rate in a blood pressure measurement. An inflatable bladder of the blood pressure measurement device defines, at least in part, a pressurizable volume. The inflatable bladder may be inflated to pressurize a user's appendage and temporarily occlude blood flow in the user's appendage. A pressure sensor of the blood pressure measurement device is configured to obtain blood pressure measurements, and a pump of the blood pressure measurement device is configured to inflate the inflatable bladder and control an inflation rate by controlling at least one of a duty cycle, a voltage, or a drive frequency.

Abstract: An aspect of the disclosure pertains to finger blood pressure cuff. The finger blood pressure cuff may include a housing having an opening sized to receive a human finger and an inflatable elastic bladder disposed about an inward-facing surface of the opening. The inflatable elastic bladder may be inflated to pressurize a user's finger and temporarily occlude blood flow in the user's finger. The finger blood pressure cuff may include a pressure sensor for obtaining blood pressure measurements.

Abstract: An aspect of the disclosure pertains to a blood pressure measurement device and methods of obtaining pulse information from a blood pressure measurement. An inflatable bladder defines, at least in part, a pressurizable volume. The inflatable bladder may be inflated to pressurize a user's appendage and temporarily occlude blood flow in the user's appendage, where the inflatable bladder is inflated to a pressure greater than a maximum amplitude pressure from oscillometric data in a pressure profile. The inflatable bladder may be deflated and then re-inflated to a target pressure and held at the target pressure to generate pulse information, or the inflatable bladder may be deflated to the target pressure and held at the target pressure to generate the pulse information.

Abstract: An aspect of the disclosure pertains to detecting a position of a blood pressure measurement device. An inflatable bladder of the blood pressure measurement device defines, at least in part, a pressurizable volume. The inflatable bladder may be inflated to pressurize a user's appendage and temporarily occlude blood flow in the user's appendage. A pump may initiate inflation of the inflatable bladder when one or more accelerometers and/or one or more proximity sensors determine that the blood pressure measurement device is within sufficient proximity or elevation to a user's heart and stationary.

Abstract: The present invention is directed to a polymerase activity assay that produces a strong optical signal when a primer-template complex is extended to full-length product. The assay uses Cy3 as the molecular beacon and Iowa Black® RQ as the quencher. The signal-to-noise-ratio (STNR) of this donor-quencher pairing is ˜200-fold over background, which is considerably better than other donor-quencher pairs (STNRs ˜10-20-fold). The STNR allows for solution-based monitoring of polymerase activity. Because the sensor functions via Watson-Crick base pairing, the polymerase activity assay may also be used to evolve polymerases to accept xeno nucleic acids as substrates.

Abstract: Biometric monitoring devices, including various technologies that may be implemented in such devices, are discussed herein. Additionally, techniques for utilizing gyroscopes in biometric monitoring devices are provided. Such techniques may, in some implementations, involve obtaining swimming metrics regarding stroke cycle count, lap count, and stroke type. Such techniques may also, in some implementations, involve obtaining performance metrics for bicycling activities.

Abstract: In one embodiment, a data processing method comprises obtaining one or more first photoplethysmography (PPG) signals based on one or more first light sources that are configured to emit light having a first light wavelength corresponding to a green light wavelength; obtaining one or more second PPG signals based on one or more second light sources that are configured to emit light having a second light wavelength corresponding to a red light wavelength, one or more of the first light sources and one or more of the second light sources being co-located; generating an estimated heart rate value based on one or more of the first PPG signals and the second PPG signals; and causing the estimated heart rate value to be displayed via a user interface on a client device.

Abstract: The present invention provides methods for rapidly screening and measuring the ligand binding affinity of in vitro selected peptides to the cognate and off-target proteins. This general strategy is amenable to high throughput analysis because the peptides are synthesized by cell-free translation, as opposed to solid-phase synthesis required by traditional assays, and affinities can be readily measured in standard formats.

Abstract: Embodiments of the present invention relate to test management using distributed computing. A computing device transmits a test job to a test client for servicing, wherein the test client has an idle resource. The computing device receives results of the test job servicing from the test client, wherein the test job includes computer code that is under development and predefined information that reflects how the test client is to execute the test job.

Abstract: Embodiments of the present invention relate to test management using distributed computing. A computing device transmits a test job to a test client for servicing, wherein the test client has an idle resource. The computing device receives results of the test job servicing from the test client, wherein the test job includes computer code that is under development and predefined information that reflects how the test client is to execute the test job.

Abstract: The present invention provides methods for rapidly screening and measuring the ligand binding affinity of in vitro selected peptides to the cognate and off-target proteins. This general strategy is amenable to high throughput analysis because the peptides are synthesized by cell-free translation, as opposed to solid-phase synthesis required by traditional assays, and affinities can be readily measured in standard formats.