Abstract: The present disclosure relates to methods of and systems for modifying the transcriptional regulation of stem or progenitor cells to promote their differentiation or reprogramming of somatic cells. Further, the labeling and editing of human genomic loci in live cells with three orthogonal CRISPR/Cas9 components allow multicolor detection of genomic loci with high spatial resolution, which provides an avenue for barcoding elements of the human genome in the living state.

Abstract: The present invention utilizes a Cas9 nickase which nicks a flanking target sequence to a duplicated gene sequence (e.g., a retroviral LTR). This nicking causes a genomic collapse of the sequence between the nick and the LTR, thereby deleting the sequence from the genome. Because the nickase does not introduce mutations at the target site, this method can be repeated maximize the efficiency (e.g., 100% of retroviral genome excision. For example, this method is useful to delete all PERVs within a pig genome intended for human transplantation. Further, such PERV-free cells can then be used to clone PERV-free pigs. Furthermore, this method is useful to remove amplified gene repeats in cancer cells.

Abstract: A method of leveraging a dialogue history of a conversational computing interface to execute an updated dialogue plan. The method comprises maintaining an annotated dialogue history of the conversational computing interface. The annotated dialogue history includes a plurality of traced steps defining a data-flow including input data used to execute a context-dependent operation and output data recorded from a previous execution of the context-dependent operation. The method further comprises recognizing an updated dialogue plan including a prefix of executable steps and an updated executable step following the prefix. The method further comprises automatically computer-recognizing that the prefix of executable steps of the updated dialogue plan matches a corresponding prefix of traced steps in the annotated dialogue history. The method further comprises re-using the data-flow from the prefix of traced steps in the annotated dialogue history to automatically determine input data of the updated executable step.

Abstract: A method includes receiving an utterance at a computerized automated assistant system, and detecting, via a date/time constraint module of the computerized automated assistant system, one or more constraints in the utterance associated with a date or time. The utterance is associated with a domain. The method further comprises generating, via the date/time constraint module, a periodic set for each of the one or more constraints associated with the date or time, and combining, via the date/time constraint module, the one or more periodic sets. The method further comprises processing, via a dialogue manager module of the computerized automated assistant system, the combined periodic sets to determine an action, and executing the action at the computerized automated assistant system.

Abstract: A method of adapting a computerized assistant program to satisfy an updated constraint. The method comprises maintaining a dialogue history including a first utterance that indicates an initial constraint. The method further comprises receiving a second utterance indicating a new constraint that conflicts with the initial constraint. The method further comprises recognizing a revision function statement parametrized by a reference to an initial computerized assistant program configured to satisfy the initial constraint, and a reference to the new constraint. The method further comprises executing instructions derived from the revision function statement to return a revised computerized assistant program that is configured to satisfy the new constraint.

Abstract: A matchmaking service can match a satellite owner/operator with antennas that are available and capable of servicing a communication request from the satellite owner/operator (e.g., to exchange data with the satellite owner/operator's satellite). The communication request may be received at a ground station service, and information included therein may be compared to capabilities of antennas registered with the ground station service to determine antennas that are compatible with the request. The ground station service may reserve a time slot for a selected compatible antenna to service the request and control the selected compatible antenna (e.g., to track and communicate with the satellite) during the time slot. In some embodiments, the matchmaking of satellite owners/operators to antennas uses models to determine communication parameters for a communication request and to determine capabilities of antennas registered with the ground station.

Abstract: A method of leveraging a dialogue history of a conversational computing interface to execute an updated dialogue plan. The method comprises maintaining an annotated dialogue history of the conversational computing interface. The annotated dialogue history includes a plurality of traced steps defining a data-flow including input data used to execute a context-dependent operation and output data recorded from a previous execution of the context-dependent operation. The method further comprises recognizing an updated dialogue plan including a prefix of executable steps and an updated executable step following the prefix. The method further comprises automatically computer-recognizing that the prefix of executable steps of the updated dialogue plan matches a corresponding prefix of traced steps in the annotated dialogue history. The method further comprises re-using the data-flow from the prefix of traced steps in the annotated dialogue history to automatically determine input data of the updated executable step.

Abstract: A method comprising recognizing a user utterance including an ambiguity. The method further comprises using a previously-trained code-generation machine to produce, from the user utterance, a data-flow program including a search-history function. The search-history function is configured to select a highest-confidence disambiguating concept from one or more candidate concepts stored in a context-specific dialogue history.

Abstract: The present disclosure relates to methods of and systems for modifying the transcriptional regulation of stem or progenitor cells to promote their differentiation or reprogramming of somatic cells. Further, the labeling and editing of human genomic loci in live cells with three orthogonal CRISPR/Cas9 components allow multicolor detection of genomic loci with high spatial resolution, which provides an avenue for barcoding elements of the human genome in the living state.

Abstract: A matchmaking service can match a satellite owner/operator with antennas that are available and capable of servicing a communication request from the satellite owner/operator (e.g., to exchange data with the satellite owner/operator's satellite). The communication request may be received at a ground station service, and information included therein may be compared to capabilities of antennas registered with the ground station service to determine antennas that are compatible with the request. The ground station service may reserve a time slot for a selected compatible antenna to service the request and control the selected compatible antenna (e.g., to track and communicate with the satellite) during the time slot. In some embodiments, the matchmaking of satellite owners/operators to antennas uses models to determine communication parameters for a communication request and to determine capabilities of antennas registered with the ground station.

Abstract: A method, comprising recognizing a user utterance for processing. The method further comprises using a previously-trained code-generation machine to generate, from the user utterance, a data-flow program configured to produce a return value upon successful execution. The method further comprises beginning execution of the data-flow program. Responsive to reaching an error condition resulting from execution of the data-flow program, the method further comprises, prior to the data-flow program producing the return value, suspending execution of the data flow program. The method further comprises using the previously-trained code-generation machine to generate an error-handling data-flow program, wherein the error-handling data-flow program is configured to produce the return value; beginning execution of the error-handling data-flow program to produce the return value; and outputting the return value.

Abstract: The present invention provides a Cas9 platform to facilitate single-site nuclease gene editing precision within a human genome. For example, a Cas9 nuclease/DNA-targeting unit (Cas9-DTU) fusion protein precisely delivers a Cas9/sgRNA complex to a specific target site within the genome for subsequent sgRNA-dependent cleavage of an adjacent target sequence. Alternatively, attenuating Cas9 binding using mutations to the a protospacer adjacent motif (PAM) recognition domain makes Cas9 target site recognition dependent on the associated DTU, all while retaining Cas9's sgRNA-mediated DNA cleavage fidelity. Cas9-DTU fusion proteins have improved target site binding precision, greater nuclease activity, and a broader sequence targeting range than standard Cas9 systems. Existing Cas9 or sgRNA variants (e.g., truncated sgRNAs (tru-gRNAs), nickases and FokI fusions) are compatible with these improvements to further reduce off-target cleavage.

Abstract: A method includes receiving an utterance at a computerized automated assistant system, and detecting, via a date/time constraint module of the computerized automated assistant system, one or more constraints in the utterance associated with a date or time. The utterance is associated with a domain. The method further comprises generating, via the date/time constraint module, a periodic set for each of the one or more constraints associated with the date or time, and combining, via the date/time constraint module, the one or more periodic sets. The method further comprises processing, via a dialogue manager module of the computerized automated assistant system, the combined periodic sets to determine an action, and executing the action at the computerized automated assistant system.

Abstract: A method comprising recognizing a user utterance including an ambiguity. The method further comprises using a previously-trained code-generation machine to produce, from the user utterance, a data-flow program including a search-history function. The search-history function is configured to select a highest-confidence disambiguating concept from one or more candidate concepts stored in a context-specific dialogue history.

Abstract: A method, comprising recognizing a user utterance for processing. The method further comprises using a previously-trained code-generation machine to generate, from the user utterance, a data-flow program configured to produce a return value upon successful execution. The method further comprises beginning execution of the data-flow program. Responsive to reaching an error condition resulting from execution of the data-flow program, the method further comprises, prior to the data-flow program producing the return value, suspending execution of the data flow program. The method further comprises using the previously-trained code-generation machine to generate an error-handling data-flow program, wherein the error-handling data-flow program is configured to produce the return value; beginning execution of the error-handling data-flow program to produce the return value; and outputting the return value.

Abstract: A method includes receiving an utterance at a computerized automated assistant system, and detecting, via a date/time constraint module of the computerized automated assistant system, one or more constraints in the utterance associated with a date or time. The utterance is associated with a domain. The method further comprises generating, via the date/time constraint module, a periodic set for each of the one or more constraints associated with the date or time, and combining, via the date/time constraint module, the one or more periodic sets. The method further comprises processing, via a dialogue manager module of the computerized automated assistant system, the combined periodic sets to determine an action, and executing the action at the computerized automated assistant system.

Abstract: Techniques for cloud data backup are disclosed. Example methods may store backup data from a client computer on portions of data storage media associated with peer computers. In some example embodiments, a file may be encoded into segments using an error-tolerant encoding scheme, and the segments may be stored on peer computers. In some example embodiments, individual segments may be stored on more than one peer machine and/or an individual peer machine may not receive all of the segments associated with the file.

Abstract: A method for a dialogue system includes establishing a dialogue session between an application executing on a server and a remote machine. The dialogue session includes one or more utterances received from a user at the remote machine. A natural language processing machine identifies a request associated with a computer-readable representation of an utterance. A dialogue expansion machine generates a plurality of alternative actions for responding to the request. A previously-trained machine learning confidence model assesses a confidence score for each alternative. If a highest confidence score for a top alternative does not satisfy a threshold, the plurality of alternatives including the top alternative are transmitted to a remote machine (which may be the same remote machine or a different remote machine) for review by a human reviewer. After the dialogue system and/or the human reviewer select an alternative, computer-readable instructions defining the selected alternative are executed.

Abstract: A method for a dialogue system includes establishing a dialogue session between an application executing on a server and a remote machine. The dialogue session includes one or more utterances received from a user at the remote machine. A natural language processing machine identifies a request associated with a computer-readable representation of an utterance. A dialogue expansion machine generates a plurality of alternative actions for responding to the request. A previously-trained machine learning confidence model assesses a confidence score for each alternative. If a highest confidence score for a top alternative does not satisfy a threshold, the plurality of alternatives including the top alternative are transmitted to a remote machine (which may be the same remote machine or a different remote machine) for review by a human reviewer. After the dialogue system and/or the human reviewer select an alternative, computer-readable instructions defining the selected alternative are executed.

Abstract: The present disclosure provides methods of treating polycystic ovary syndrome (PCOS) with tetrahydro-iso-alpha acid (THIAA) derivatives and substantially enantiomerically pure compositions and pharmaceutical formulations thereof, in particular KDT500 and KDT501.