Abstract: The present invention provides a Cas9 platform to facilitate single-site nuclease gene editing precision within a human genome. For example, a Cas9 nuclease/DNA-targeting unit (Cas9-DTU) fusion protein precisely delivers a Cas9/sgRNA complex to a specific target site within the genome for subsequent sgRNA-dependent cleavage of an adjacent target sequence. Alternatively, attenuating Cas9 binding using mutations to the a protospacer adjacent motif (PAM) recognition domain makes Cas9 target site recognition dependent on the associated DTU, all while retaining Cas9's sgRNA-mediated DNA cleavage fidelity. Cas9-DTU fusion proteins have improved target site binding precision, greater nuclease activity, and a broader sequence targeting range than standard Cas9 systems. Existing Cas9 or sgRNA variants (e.g., truncated sgRNAs (tru-gRNAs), nickases and FokI fusions) are compatible with these improvements to further reduce off-target cleavage.

Abstract: A method includes receiving an utterance at a computerized automated assistant system, and detecting, via a date/time constraint module of the computerized automated assistant system, one or more constraints in the utterance associated with a date or time. The utterance is associated with a domain. The method further comprises generating, via the date/time constraint module, a periodic set for each of the one or more constraints associated with the date or time, and combining, via the date/time constraint module, the one or more periodic sets. The method further comprises processing, via a dialogue manager module of the computerized automated assistant system, the combined periodic sets to determine an action, and executing the action at the computerized automated assistant system.

Abstract: An intervertebral scaffolding system is provided having a laterovertically-expanding frame operable for a reversible collapse from an expanded state into a collapsed state, the laterovertically-expanding frame having a stabilizer, one or more tensioners, or a combination of the stabilizer with one or more tensioners. The stabilizer slidably engages with the distal region of the laterovertically-expanding frame and both the stabilizer and the one or more tensioners are configured for retaining the laterovertically-expanding frame from a lateral movement that exceeds the expanded state, and the stabilizer can include a locking element that engages with the expansion member to lock the expansion member to the stabilizer when the expansion member is fully inserted into the frame. The expanded state, for example, can be configured to have an open graft distribution window that at least substantially closes upon the reversible collapse.

Abstract: An intervertebral scaffolding system is provided having a laterovertically-expanding frame operable for a reversible collapse from an expanded state into a collapsed state, the laterovertically-expanding frame having a stabilizer, one or more tensioners, or a combination of the stabilizer with one or more tensioners. The stabilizer slidably engages with the distal region of the laterovertically-expanding frame and both the stabilizer and the one or more tensioners are configured for retaining the laterovertically-expanding frame from a lateral movement that exceeds the expanded state, and the stabilizer can include a locking element that engages with the expansion member to lock the expansion member to the stabilizer when the expansion member is fully inserted into the frame. The expanded state, for example, can be configured to have an open graft distribution window that at least substantially closes upon the reversible collapse.

Abstract: Techniques for cloud data backup are disclosed. Example methods may store backup data from a client computer on portions of data storage media associated with peer computers. In some example embodiments, a file may be encoded into segments using an error-tolerant encoding scheme, and the segments may be stored on peer computers. In some example embodiments, individual segments may be stored on more than one peer machine and/or an individual peer machine may not receive all of the segments associated with the file.

Abstract: A method includes receiving an utterance at a computerized automated assistant system, and detecting, via a date/time constraint module of the computerized automated assistant system, one or more constraints in the utterance associated with a date or time. The utterance is associated with a domain. The method further comprises generating, via the date/time constraint module, a periodic set for each of the one or more constraints associated with the date or time, and combining, via the date/time constraint module, the one or more periodic sets. The method further comprises processing, via a dialogue manager module of the computerized automated assistant system, the combined periodic sets to determine an action, and executing the action at the computerized automated assistant system.

Abstract: Devices and methods are provided herein for locating and monitoring subcutaneous target sites. A device as described herein may comprise a base layer configured to attach to a portion of a subject's body in proximity to the target site; and a plurality of transducer elements on the base layer, wherein the plurality of transducer elements are configured to (1) transmit a set of signals that penetrate beneath the skin of the subject and (2) receive a set of reflected signals associated with underlying anatomical structures, the underlying anatomical structures comprising the target site and bodily tissue surrounding the target site, wherein the set of reflected signals is used to identify a location of the target site while the device is placed in situ on the subject's body, without having to physically move the device over the portion of the subject's body to search for the target site.

Abstract: A method for a dialogue system includes establishing a dialogue session between an application executing on a server and a remote machine. The dialogue session includes one or more utterances received from a user at the remote machine. A natural language processing machine identifies a request associated with a computer-readable representation of an utterance. A dialogue expansion machine generates a plurality of alternative actions for responding to the request. A previously-trained machine learning confidence model assesses a confidence score for each alternative. If a highest confidence score for a top alternative does not satisfy a threshold, the plurality of alternatives including the top alternative are transmitted to a remote machine (which may be the same remote machine or a different remote machine) for review by a human reviewer. After the dialogue system and/or the human reviewer select an alternative, computer-readable instructions defining the selected alternative are executed.

Abstract: A therapeutic support device includes a bladder having one or more independently inflatable compartments, each including a plurality of inflatable cells. When inflated, each inflatable cell forms a contact node that may support a user or another object disposed thereon. The inflatable compartments can be alternately inflated and deflated such that contact pressure can be applied to and relieved from corresponding portions of the user's body in an alternating manner.

Abstract: A method for a dialogue system includes establishing a dialogue session between an application executing on a server and a remote machine. The dialogue session includes one or more utterances received from a user at the remote machine. A natural language processing machine identifies a request associated with a computer-readable representation of an utterance. A dialogue expansion machine generates a plurality of alternative actions for responding to the request. A previously-trained machine learning confidence model assesses a confidence score for each alternative. If a highest confidence score for a top alternative does not satisfy a threshold, the plurality of alternatives including the top alternative are transmitted to a remote machine (which may be the same remote machine or a different remote machine) for review by a human reviewer. After the dialogue system and/or the human reviewer select an alternative, computer-readable instructions defining the selected alternative are executed.

Abstract: Provided is a computer-implemented method for providing an electronic funds transfer (EFT) based on issuer system requirements that includes receiving an EFT request associated with an account, the EFT request associated with a transfer of an amount of funds to the account, determining whether an issuer system supports the transfer to the account according to a first electronic fund transfer protocol, determining second electronic fund transfer protocol settings data associated with performing the transfer to the account using the second electronic fund transfer protocol based on determining that the issuer system that issued the account of the user does not support the transfer to the account of the user according to the first electronic fund transfer protocol, and processing the transfer to the account in real-time based on the second electronic fund transfer protocol settings data. A system and computer program product are also disclosed.

Abstract: A portable fire pit is provided that includes a frame, a support structure, and a mesh. The frame can include a plurality of upwardly extending rods and cross-bars extending between the upwardly extending rods. The support structure can include a plurality of supports having an upper wall, a base, and an aperture sized to receive an upwardly extending rod of the frame. The support structure can extend around a periphery of the frame.

Abstract: An intervertebral scaffolding system is provided having a laterovertically-expanding frame operable for a reversible collapse from an expanded state into a collapsed state, the laterovertically-expanding frame having a stabilizer, one or more tensioners, or a combination of the stabilizer with one or more tensioners. The stabilizer slidably engages with the distal region of the laterovertically-expanding frame and both the stabilizer and the one or more tensioners are configured for retaining the laterovertically-expanding frame from a lateral movement that exceeds the expanded state, and the stabilizer can include a locking element that engages with the expansion member to lock the expansion member to the stabilizer when the expansion member is fully inserted into the frame. The expanded state, for example, can be configured to have an open graft distribution window that at least substantially closes upon the reversible collapse.

Abstract: A vented fire tarp is provided that includes a base portion and a plurality of vents formed in the base portion, wherein each vent allows for air, smoke, and carbon dioxide to pass through the vent from one side of the base portion to the other. The vented fire tarp also includes securing devices to couple the base portion to a ground surface over a fire. In use, the vented fire tarp covers a fire and controls a burn rate of wood of the fire in response to controlling airflow through the plurality of vents of the vented fire tarp.