Abstract: The present invention relates to variant Cas12i4 polypeptides, methods of producing the variant Cas12i4 polypeptides, processes for characterizing the variant Cas12i4 polypeptides, cells comprising the variant Cas12i4 polypeptides, and methods of using the variant Cas12i4 polypeptides. The invention further relates to complexes comprising a variant Cas12i4 polypeptide and an RNA guide, methods of producing the complexes, processes for characterizing the complexes, cells comprising the complexes, and methods of using the complexes.

Abstract: The present invention relates to variant polypeptides, methods of preparing the variant polypeptides, processes for characterizing the variant polypeptides, compositions and cells comprising the variant polypeptides, and methods of using the variant polypeptides. The invention further relates to complexes comprising the variant polypeptides, methods of producing the complexes, processes for characterizing the complexes, cells comprising the complexes, and methods of using the complexes.

Abstract: A subsurface representation that includes geometry and material properties of subsurface geological formations may be generated. To compute the initial (pre-production) state of stress, gravitational forces and tectonic forces may be applied to the subsurface representation. These loads may result in stress and displacement fields within the subsurface representation. The stress field may be calibrated against available measurements. At this point, the subsurface representation may be reinitialized by resetting the displacements within the subsurface representation while maintaining the stress field.

Abstract: A representative embodiment of a lighting system includes a carrying bag having a first opening with a control panel cover; a mounting bracket arranged in the carrying bag; a power supply coupled to or arranged on the mounting bracket; a control panel arranged within the first opening and coupled to the mounting bracket; a first flexible light strip; a second flexible light strip; and a power cable coupleable within the carrying bag to the control panel, the first flexible light strip, and the second flexible light strip. The first and second flexible light strips are coupleable to the power cable to receive DC power from the control panel, and may be extended throughout a structure, such as a tent, using a plurality of hangers. In a representative embodiment, the first flexible light strip provides white light and the second flexible light strip provides blackout light.

Abstract: A packaging substrate can include a first surface and a second opposing surface, the first surface including a first mounting region of a first electronic module region and the second opposing surface including a first electrical contacts region of the first electronic module region. The packaging substrate can include a saw street region with at least a portion that surrounds the first electronic module region, and a saw street feature formed on the second opposing surface within at least a portion of the saw street region, the saw street feature being a solder mask layer over a metal layer.

Abstract: Provided herein are pharmaceutically acceptable sodium nitrite and pharmaceutical compositions thereof. Also provided herein are methods for determining the total non-volatile organic carbon in a sodium nitrite-containing sample. Further provided herein are methods for producing pharmaceutically acceptable sodium nitrite. Still further provided herein are methods of treatment comprising the administration of pharmaceutically acceptable sodium nitrite.

Abstract: A system for removing particulate matter from a multiphase stream comprising gas, liquid and the particulate matter. The system comprises a first vessel for receiving the multiphase stream and separating a majority of gas from the multiphase stream and collecting a slurry of liquid and particulate matter; a second vessel for receiving the slurry and causing separation of the particulate matter from the liquid and for generating a pressure head of liquid against the particulate matter; a third vessel for receiving the particulate matter from the second vessel and collecting the particulate matter until a pre-determined mass or volume of particulate matter is collected; and an outlet in the third vessel for conveying the particulate matter out of the third vessel.

Abstract: The present disclosure relates to variant polypeptides, methods of preparing the variant polypeptides, processes for characterizing the variant polypeptides, compositions and cells comprising the variant polypeptides, and methods of using the variant polypeptides. The disclosure further relates to complexes comprising the variant polypeptides, methods of producing the complexes, processes for characterizing the complexes, cells comprising the complexes, and methods of using the complexes.

Abstract: The present disclosure relates to cells modified by a Cas12i polypeptide, methods of modifying the cells, processes for characterizing the modified cells, compositions and formulations comprising the modified cells, and uses of the compositions and formulations comprising the modified cells.

Abstract: In various embodiments, a goods to operator workstation is provided. The goods to operator workstation include an upper level. The upper level includes an upper level conveyor and one or more upper level container bays configured to receive and present one of the one or more upper level containers. The goods to operator workstation also includes at least one connector panel extending from the upper level. The goods to operator workstation further includes a lower level coupled to the at least one connector panel. The lower level is configured to receive one or more lower level containers.

Abstract: A bin volume predictor can receive data characterizing measurements of a temperature at a plurality of temperature sensors of a temperature sensor cable that is positioned to extend along a height of the storage bin in an interior of the storage bin and data characterizing measurements of an ambient temperature of an exterior of the storage bin. The bin volume predictor identifies, based on the measured temperature for temperature sensors in the interior of the storage bin, a topmost covered temperature sensor of the temperature sensor cable. Moreover, the bin volume predictor determines, based on the identified topmost covered temperature sensor, an amount of material stored in the storage bin.

Abstract: The technology disclosed directly operates on sequencing data and derives its own feature filters. It processes a plurality of aligned reads that span a target base position. It combines elegant encoding of the reads with a lightweight analysis to produce good recall and precision using lightweight hardware. For instance, one million training examples of target base variant sites with 50 to 100 reads each can be trained on a single GPU card in less than 10 hours with good recall and precision. A single GPU card is desirable because it a computer with a single GPU is inexpensive, almost universally within reach for users looking at genetic data. It is readily available on could-based platforms.

Abstract: The medical field of COVID-19 diagnosis relates to methods for detecting SARS-CoV-2 nucleic acids in a sample as well as combinations of oligomers for determining the presence or absence of SARS-CoV-2 in a Sample.

Abstract: A system for genetic editing of a stathmin 2 (STMN2) gene, comprising (i) a Cas12i2 polypeptide or a first nucleic acid encoding the Cas12i2 polypeptide, and (ii) an RNA guide or a second nucleic acid encoding the RNA guide, wherein the RNA guide comprises a spacer sequence specific to a target sequence within an STMN2 gene. Also provided herein are methods for editing a STMN2 gene using the gene editing system disclosed herein and/or for treating diseases associated with the STMN2 gene.

Abstract: An explosives delivery vehicle for delivering a booster for initiating an explosion of an explosive material in a hole in a floor of a mine pit to an operative depth in the hole. The vehicle comprises a storage assembly for storing a plurality of the boosters, a booster loading assembly for (i) supporting the booster in a delivery position above the hole and (ii) moving the booster downwardly into the hole and inserting the booster at an operative depth in the hole; and a delivery assembly for transporting the booster from the storage assembly to the loading assembly.

Abstract: A control system includes a refrigerant recovery module and at least one of a valve control module and a compressor control module. The refrigerant recovery module is configured to generate a refrigerant recovery signal to initiate a recovery of refrigerant from a first heat exchanger of a thermal system for an electric vehicle, and to stop the refrigerant recovery based on a temperature of refrigerant circulating through the first heat exchanger. The valve control module is configured to open a first valve to allow refrigerant to flow through the first heat exchanger in response to the refrigerant recovery signal. The compressor control module is configured to increase a speed of a compressor disposed upstream from the first heat exchanger in response to the refrigerant recovery signal.

Abstract: A thermal management system for a vehicle includes a plurality of fluid flow circuits including a heating ventilation and air conditioning (HVAC) circuit circulating a flow of refrigerant therethrough and including an evaporator, a chiller heat exchanger, a first expansion valve located upstream of the evaporator, a second expansion valve located upstream of the chiller heat exchanger, and a heat exchanger located fluidly upstream of the expansion valves. A propulsion cooling circuit circulates a flow of coolant therethrough which is utilized to condition one or more propulsion components of the vehicle. The flow of coolant is directed through the heat exchanger, thus subcooling the flow of refrigerant. A controller is operably connected to one or more control points of the thermal management system and is configured to adjust the one or more control points to achieve a target amount of subcooling of the flow of refrigerant at the heat exchanger.

Abstract: Packaged modules for use in wireless devices are disclosed. A substrate supports integrated circuit die including at least a portion of a baseband system and a front end system, an oscillator assembly, and an antenna. The oscillator assembly includes an enclosure to enclose the oscillator and conductive pillars formed at least partially within a side of the enclosure to conduct signals between the top and bottom surfaces of the oscillator assembly. Components can be vertically integrated to save space and reduce trace length. Vertical integration provides an overhang volume that can include discrete components. Radio frequency shielding and ground planes within the substrate shield the front end system and antenna from radio frequency interference. Stacked filter assemblies include passive surface mount devices to filter radio frequency signals.

Abstract: Devices for fabrication of shielded modules. In some embodiments, a carrier assembly can be provided for processing of packaged modules. The carrier assembly can include a plate having a first side that defines a plurality of openings, and an adhesive layer implemented on the first side of the plate. The adhesive layer can define a plurality of openings arranged to substantially match the openings of the plate, with each opening of the adhesive layer being dimensioned such that the adhesive layer is capable of providing an adhesive engagement between an underside perimeter portion of a package and a perimeter portion about the corresponding opening of the first side of the plate.