Patents by Inventor Anthony P. Shuber

Anthony P. Shuber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
    Patent number: 6818404
    Abstract: The invention provides amplification-based methods for detecting hypermethylated nucleic acid in heterogeneous biological samples, e.g., stool. A screening procedure based on the detection of hypermethylation, preferably at multiple genes, provides a means for detecting various diseases, e.g., colorectal cancer. By using chimeric primers that contain a 5′ non-specific portion, the specificity and efficiency of the nucleic acid amplification is improved. Methods of the invention are especially useful in detection of rare events in a heterogeneous sample.
    Type: Grant
    Filed: April 11, 2002
    Date of Patent: November 16, 2004
    Assignee: Exact Sciences Corporation
    Inventor: Anthony P. Shuber
  • Method for alteration detection
    Patent number: 6750020
    Abstract: Methods are provided for detecting an alteration in a target nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease.
    Type: Grant
    Filed: November 20, 2001
    Date of Patent: June 15, 2004
    Assignee: Exact Sciences Corporation
    Inventor: Anthony P. Shuber
  • Method for alteration detection
    Publication number: 20040110179
    Abstract: Methods are provided for detecting an alteration or the absence of an alteration in a target nucleic acid. Methods of the invention are useful for detecting and identifying mutations that are indicative of disease or the predisposition for disease. Methods of the invention involve annealing a series of probes to a target nucleic acid and detecting the presence of a mutation in said target nucleic acid if one of the probes fails to anneal, thereby exposing a single stranded stretch of nucleic acid that is susceptible to degradation.
    Type: Application
    Filed: September 9, 2003
    Publication date: June 10, 2004
    Inventor: Anthony P. Shuber
  • Primer extension methods for detecting nucleic acids
    Publication number: 20040110162
    Abstract: Methods are provided for selective nucleic acid sequence detection in single base primer extension reactions of high sensitivity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.
    Type: Application
    Filed: February 5, 2003
    Publication date: June 10, 2004
    Applicant: EXACT SCIENCES CORPORATION
    Inventors: Anthony P. Shuber, Stanley N. Lapidus
  • Supracolonic aerodigestive neoplasm detection
    Publication number: 20040043467
    Abstract: The invention provides methods and materials for detecting supracolonic aerodigestive premalignant and malignant neoplasms. Specifically, the invention provides methods and materials for determining whether a stool sample from a mammal contains a neoplasm-specific marker from a neoplsm located in the supracolonic aerodigestive tissue of a mammal.
    Type: Application
    Filed: March 18, 2003
    Publication date: March 4, 2004
    Inventors: Anthony P. Shuber, David A. Ahlquist
  • Methods for disease detection
    Publication number: 20040014104
    Abstract: The present invention provides methods for detecting disease by analysis of a patient sample to determine the integrity of nucleic acids in the sample.
    Type: Application
    Filed: May 7, 2003
    Publication date: January 22, 2004
    Inventor: Anthony P. Shuber
  • Methods for analysis of molecular events
    Publication number: 20030203382
    Abstract: Methods and compositions are provided for detecting the presence of nucleic acid sequence variants in a subpopulation of nucleic acid molecules in a biological sample. These methods are particularly useful for identifying individuals with mutations indicative of cancer.
    Type: Application
    Filed: February 18, 2003
    Publication date: October 30, 2003
    Applicant: Exact Sciences Corporation
    Inventors: Anthony P. Shuber, Lisa Kann, Duncan Whitney
  • Automated sample preparation methods and devices
    Publication number: 20030204331
    Abstract: The invention provides methods and apparatus for automated sample processing. The invention comprises introducing a sample into a multistep dilution procedure at a step that is chosen based on the concentration of target analyte in the sample.
    Type: Application
    Filed: November 20, 2002
    Publication date: October 30, 2003
    Inventors: Duncan Whitney, Anthony P. Shuber, James Kent Moore
  • Methods for disease detection
    Patent number: 6586177
    Abstract: The present invention provides methods for detecting disease by analysis of a patient sample to determine the integrity of nucleic acids in the sample.
    Type: Grant
    Filed: December 7, 1999
    Date of Patent: July 1, 2003
    Assignee: Exact Sciences Corporation
    Inventor: Anthony P. Shuber
  • Primer extension methods for detecting nucleic acids
    Patent number: 6566101
    Abstract: Methods are provided for selective nucleic acid sequence detection in single base primer extension reactions of high sensitivity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.
    Type: Grant
    Filed: April 27, 1998
    Date of Patent: May 20, 2003
    Inventors: Anthony P. Shuber, Stanley N. Lapidus
  • Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
    Publication number: 20030087258
    Abstract: The invention provides amplification-based methods for detecting hypermethylated nucleic acid in heterogeneous biological samples, e.g., stool. A screening procedure based on the detection of hypermethylation, preferably at multiple genes, provides a means for detecting various diseases, e.g., colorectal cancer. By using chimeric primers that contain a 5′ non-specific portion, the specificity and efficiency of the nucleic acid amplification is improved. Methods of the invention are especially useful in detection of rare events in a heterogeneous sample.
    Type: Application
    Filed: April 11, 2002
    Publication date: May 8, 2003
    Inventor: Anthony P. Shuber
  • Methods for preserving DNA integrity
    Patent number: 6551777
    Abstract: Methods for extracting DNA from a biological sample that result in a higher yield of target DNA than conventional methods. More particularly, methods for extracting DNA include exposing the biological sample to inhibitors of DNA degradation.
    Type: Grant
    Filed: January 24, 2000
    Date of Patent: April 22, 2003
    Assignee: Exact Sciences Corporation
    Inventors: Anthony P. Shuber, Frederick A. Huntress, Jr., James K. Moore
  • PRIMER EXTENSION METHODS UTILIZING DONOR AND ACCEPTOR MOLECULES FOR DETECTING NUCLEIC ACIDS
    Publication number: 20030044780
    Abstract: Methods are provided for selective nucleic acid sequence detection in primer extension reactions of high specificity. These methods are useful for detecting small amounts of mutant nucleic acid in a heterogeneous biological sample. These methods are particularly useful for identifying individuals with gene mutations indicative of early colorectal cancer.
    Type: Application
    Filed: November 23, 1999
    Publication date: March 6, 2003
    Inventors: STANLEY N. LAPIDUS, ANTHONY P. SHUBER
  • Methods for detecting mutations using primer extension for detecting disease
    Patent number: 6503718
    Abstract: Methods of the invention comprise assays for markers indicative of cancer, precancer, and other diseases or disorders. Assays of the invention are preformed on heterogeneous samples obtained from patients by non-invasive or minimally-invasive methods.
    Type: Grant
    Filed: January 10, 2001
    Date of Patent: January 7, 2003
    Assignee: Exact Sciences Corporation
    Inventors: Anthony P. Shuber, William Pierceall, Steven J. Laken
  • Methods for detecting mutations using primer extension
    Patent number: 6482595
    Abstract: Methods for detecting nucleotide deletions in biological samples are described. Methods of the invention are particulary useful for detecting deletions in regions of polynucleotide repeats. In particular, methods of the invention are useful to detect deletions at the BAT26 locus.
    Type: Grant
    Filed: August 27, 2001
    Date of Patent: November 19, 2002
    Assignee: Exact Sciences Corporation
    Inventors: Anthony P. Shuber, William Pierceall
  • Methods for stool sample preparation
    Publication number: 20020164631
    Abstract: The present invention provides methods for the preparation of stool samples to increase the yield of relevant DNA, and further provides methods for isolating and analyzing target DNA for characteristics indicative of colorectal cancer. Methods for screening patients for the presence of cancerous or pre-cancerous colorectal lesions are also provided.
    Type: Application
    Filed: March 25, 2002
    Publication date: November 7, 2002
    Inventors: Anthony P. Shuber, Stanley N. Lapidus, Gail E. Radcliffe
  • Methods for detecting mutations using primer extension for detecting disease
    Patent number: 6475738
    Abstract: Methods of the invention comprise assays for markers indicative of cancer, precancer, and other diseases or disorders. Assays of the invention are preformed on heterogeneous samples obtained from patients by non-invasive or minimally-invasive methods. Such assays may be employed alone or in combination with other disease screening techniques.
    Type: Grant
    Filed: June 18, 2001
    Date of Patent: November 5, 2002
    Assignee: Exact Sciences Corporation
    Inventors: Anthony P. Shuber, William Pierceall
  • Method for alteration detection
    Publication number: 20020132251
    Abstract: Methods are provided for detecting an alteration in a target nucleic acid.
    Type: Application
    Filed: November 20, 2001
    Publication date: September 19, 2002
    Inventor: Anthony P. Shuber
  • Methods for the detection of nucleic acids
    Publication number: 20020119469
    Abstract: Methods are provided for identifying nucleic acids. Methods of the invention are usefull for identifying and analyzing nucleic acids, especially variants of single nucleotide polymorphisms, that are indicative of disease or the predisposition for disease.
    Type: Application
    Filed: October 5, 2001
    Publication date: August 29, 2002
    Inventors: Anthony P. Shuber, Stanley N. Lapidus
  • Methods for disease diagnosis from stool samples
    Publication number: 20020119472
    Abstract: The present invention provides methods for preparing a stool sample in order to screen for the presence of indicators of a disease, for example a subpopulation of cancerous or precancerous cells. The methods take advantage of the recognition that cellular debris from cancerous and precancerous cells is deposited onto only a longitudinal stripe of stool as the stool is forming in the colon. Accordingly, methods of the invention comprise obtaining a representative sample, such as a circumferential or cross-sectional sample of stool in order to ensure that any disease indicator, such as cellular debris that is shed by colonic cells, is obtained in the sample.
    Type: Application
    Filed: October 15, 2001
    Publication date: August 29, 2002
    Inventors: Stanley N. Lapidus, Anthony P. Shuber