Patents by Inventor Asaph Aharoni
Asaph Aharoni has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240150744Abstract: The present invention provides polynucleotide sequences derived from Helichrysum umbraculigerum and encoding a protein or a plurality thereof belonging to the acyl activating enzyme (AAE) family. Further provided are an artificial nucleic acid molecule including the polynucleotide, a transgenic cell, tissue, or plant including same. Further provided are method for synthesizing an acyl coenzyme A (CoA).Type: ApplicationFiled: March 10, 2022Publication date: May 9, 2024Inventors: Asaph AHARONI, Prashant SONAWANE, Adam JOZWIAK, Paula BERMAN, Luis DE-HARO
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Patent number: 11957102Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.Type: GrantFiled: July 7, 2022Date of Patent: April 16, 2024Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Prashant Sonawane, Maxim Itkin
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Patent number: 11932887Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as Mirabilis. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.Type: GrantFiled: July 28, 2020Date of Patent: March 19, 2024Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Guy Polturak
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Publication number: 20230034320Abstract: Disclosed herein are genetically modified plants expressing multiple animal milk proteins. Further disclosed are vectors for expressing in a plant and parts thereof, multiple mammalian milk proteins; genetically modified and or gene-edited plants having at least one cell expressing and producing at least two recombinant mammalian milk proteins wherein expression may be in a seed, bean, grain, fruit, nut, legume, leaf, stem, root, or portion thereof, the recombinant protein being produced by the plant cell; and a method of producing a food, medicament, cosmetic or blocking composition from the genetically modified or gene-edited plant. Reduction or elimination of seed storage proteins in a cell or cells wherein the milk proteins are expressed, or reduction of plant enzymes that can increase the content of oleic and/or stearic fatty acids and/or reduce the content of saturated fats in the plants or plant products are also disclosed.Type: ApplicationFiled: September 30, 2021Publication date: February 2, 2023Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Aviel EVEN, Dan EVEN
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Publication number: 20220408679Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.Type: ApplicationFiled: July 7, 2022Publication date: December 29, 2022Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Prashant SONAWANE, Maxim ITKIN
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Patent number: 11412700Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.Type: GrantFiled: September 1, 2020Date of Patent: August 16, 2022Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Prashant Sonawane, Maxim Itkin
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Publication number: 20220217934Abstract: Provided herein are genetically modified cells and genetically modified plants having increased or decreased expression of a cellulose synthase like G (CSLG) enzyme. These cells and plants may have an increased or decreased content a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin, compared to a corresponding unmodified cell or plant. Also provided herein are methods of producing a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a genetically modified cell, as well as methods of reducing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part, and methods of increasing the content of a steroidal alkaloid, a steroidal saponin, or a triterpenoid saponin in a cell of a plant or a plant part.Type: ApplicationFiled: September 5, 2019Publication date: July 14, 2022Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Prashant SONAWANE, Maxim ITKIN, Adam JOZWlAK
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Publication number: 20210348179Abstract: Disclosed herein are methods for gene targeting in a plant cell, as well as recombinant nucleic acid molecules used in these methods. Further disclosed are recombinant nucleic acid molecules comprising a target gene operably linked to a regulatory region of the UBQ10 gene.Type: ApplicationFiled: July 21, 2021Publication date: November 11, 2021Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Samuel BOCOBZA, Tal DAHAN, Avraham A. LEVY
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Patent number: 11104910Abstract: Disclosed herein are methods for gene targeting in a plant cell, as well as recombinant nucleic acid molecules used in these methods. Further disclosed are recombinant nucleic acid molecules comprising a target gene operably linked to a regulatory region of the UBQ10 gene.Type: GrantFiled: August 22, 2018Date of Patent: August 31, 2021Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Samuel Bocobza, Tal Dahan, Avraham A. Levy
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Publication number: 20210007363Abstract: Provided is a plant exudate, methods for obtaining a plant exudate by inducing the plant to secrete an exudate and systems for the collection of a plant exudate which include: one or more plant container including at least two discrete compartments each configured to accommodate a split root of a plant, the compartments being a root stimulating compartment including one or more input being in fluid communication with at least a source of a plant root stimulant, and a root exudate harvesting compartment, and a root exudate collection compartment in fluid communication with the root exudate harvesting compartment.Type: ApplicationFiled: March 21, 2019Publication date: January 14, 2021Inventors: Asaph AHARONI, Elisa KORENBLUM
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Publication number: 20200390053Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.Type: ApplicationFiled: September 1, 2020Publication date: December 17, 2020Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Prashant SONAWANE, Maxim ITKIN
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Publication number: 20200367493Abstract: The present invention relates to the identification and use of natural plant compounds for inducing, enhancing and maintaining beneficial rhizobacteria biofilm formation on a plant or plant part thereof, and conversely, inhibiting formation of biofilms and pellicle by plant pathogens. Methods of use for stimulating matrix production from the beneficial rhizobacteria and enhanced adhesion of the rhizobacteria to a plant or plant part thereof are also described. Methods of use for inhibition plant pathogen adhesion to biofilms are also described.Type: ApplicationFiled: June 6, 2017Publication date: November 26, 2020Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Ilana KOLODKIN-GAL, Asaph AHARONI
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Publication number: 20200354759Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.Type: ApplicationFiled: July 28, 2020Publication date: November 12, 2020Inventors: Asaph AHARONI, Guy POLTURAK
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Patent number: 10806119Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.Type: GrantFiled: September 6, 2018Date of Patent: October 20, 2020Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Prashant Sonawane, Maxim Itkin
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Patent number: 10767201Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4.5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.Type: GrantFiled: September 11, 2016Date of Patent: September 8, 2020Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Guy Polturak
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Publication number: 20190367940Abstract: Disclosed herein are genetically modified plants having altered biological activity of 3-?-hydroxysteroid dehydrogenase/isomerase (GAME25), or 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plants have an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof. Further disclosed herein are genetically modified plants having altered expression of a gene encoding a 3-?-hydroxysteroid dehydrogenase/isomerase (GAME25), or a 2-oxoglutarate-dependent dioxygenase (GAME31), or a combination thereof, wherein the genetically modified plant has an altered content of at least one cholesterol derived compound selected from the group including a steroidal alkaloid or a glycosylated derivative thereof and an unsaturated or saturated steroidal saponin or a glycoside derivative thereof.Type: ApplicationFiled: February 8, 2018Publication date: December 5, 2019Applicants: YEDA RESEARCH AND DEVELOPMENT CO. LTD., Yissum Research Development Company, of The Hebrew University of Jerusalem Ltd.Inventors: Asaph AHARONI, Prashant SONAWANE, Pablo D. CARDENAS, BURDMAN Saul
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Publication number: 20190059314Abstract: The present invention relates to key genes in the biosynthesis of steroidal alkaloids and saponins, including regulatory genes and enzyme-encoding genes, and to use thereof for altering the content of steroidal (glyco)alkaloids or phytosterols in plants. The present invention provides genetically modified plants or gene edited plants with altered content of steroidal (glyco)alkaloids, particularly to Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids and to the increase in phytosterols, including cholesterol or cholestanol in these plants. The present invention also provides methods of altering gene expression.Type: ApplicationFiled: September 6, 2018Publication date: February 28, 2019Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Prashant Sonawane, Maxim Itkin
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Publication number: 20190048330Abstract: Disclosed herein are methods for gene targeting in a plant cell, as well as recombinant nucleic acid molecules used in these methods. Further disclosed are recombinant nucleic acid molecules comprising a target gene operably linked to a regulatory region of the UBQ10 gene.Type: ApplicationFiled: August 22, 2018Publication date: February 14, 2019Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Samuel Bocobza, Tal Dahan, Avraham A. Levy
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Patent number: 10100322Abstract: The present invention relates to genetically modified plants by key genes involved in the biosynthesis of steroidal alkaloids. These plants have altered content of steroidal (glyco)alkaloids. Solanaceous crop plants with reduced content of antinutritional steroidal glycoalkaloids are provided.Type: GrantFiled: June 2, 2014Date of Patent: October 16, 2018Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Maxim Itkin
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Publication number: 20180251801Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.Type: ApplicationFiled: September 11, 2016Publication date: September 6, 2018Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph AHARONI, Guy Polturak