Abstract: The need to control the activity and fidelity of CRISPR-associated nucleases has resulted in the demand for inhibitory anti-CRISPR molecules. Current small-molecule inhibitor discovery platforms are not generalizable to multiple nuclease classes, only target the initial step in the catalytic activity, and require high concentration of nuclease, resulting in inhibitors with suboptimal attributes, including poor potency. Herein, Applicants report a high-throughput discovery pipeline consisting of a FRET-based assay that is generalizable to contemporary and emerging nucleases, operates at low nuclease concentration, and targets all catalytic steps. Applicants applied this pipeline to identify BRD7586, a cell-permeable small-molecule inhibitor of SpCas9, that is 2-fold more potent than current inhibitors. Furthermore, unlike the reported inhibitors, BRD7586 enhanced SpCas9 specificity and its activity was independent of the genomic loci, DNA repair pathway, or mode of nuclease delivery.

Abstract: A method and a system for determining analyte content in a fluid in a treatment apparatus. The method comprises positioning a device for treatment in a treatment apparatus. A surface of the device is contacted with a fluid. An analyte content in the fluid is determined based on an electromagnetic property of the fluid following contacting the surface of the device with the fluid. More specifically, an electromagnetic property is measured. The measuring comprises emitting electromagnetic radiation into the fluid in the treatment apparatus and detecting electromagnetic radiation from the fluid. In another aspect, a system comprising a treatment apparatus and a detector for determining the analyte content in the fluid in the treatment apparatus is provided.

Abstract: A layer of additive material is formed in a circular printing area on a substrate using additive sources distributed across a printing zone. The additive sources form predetermined discrete amounts of the additive material. The substrate and the additive sources are rotated with respect to each other around a center of rotation, so that a pattern of the additive material is formed in a circular printing area on the substrate. Each additive source receives actuation waveforms at an actuation frequency that is proportional to a distance of the additive source from the center of rotation. The actuation waveforms include formation signals, with a maximum of one formation signal in each cycle of the actuation frequency. The formation signals result in the additive sources forming the predetermined discrete amounts of the additive material on the substrate.

Abstract: A process of determining a sentiment score from a response from at least one individual regarding a status of a project that includes receiving a first numerical rating regarding the status of the project from a first individual of the at least one individual, receiving a first textual description regarding the heath of the project from the first individual, determining a first textual score dependent upon the first textual description with the first textual score being a numerical representation of the first textual description, and aggregating the first numerical rating and the first textual score to determine a first sentiment score reflective of the status of the project by the first individual.

Abstract: A method for designing an energy system is provided. An abstract structure of the energy system is determined based on a pre-defined abstract model for each one of the plurality of components. Configuration(s) of a system sizing associated with the energy system are determined. Each of the configuration(s) of the system sizing is determined based on the abstract structure and a set of requirements associated with the abstract structure of the energy system. For each of the configuration(s) of the system sizing, a respective complex structure of the energy system is determined based on a pre-defined complex model for each of the components and the abstract structure, and a performance of the respective complex structure of the energy system is determined based on a set of requirements associated with the energy system. The energy system is designed based on respective performances determined for the configuration(s) of the system sizing.

Abstract: A system includes an agricultural implement and a tractor for pulling the implement. The implement includes a frame supporting at least one ground-engaging tool and at least one image imager system coupled to a longitudinal end of the frame. The tractor includes a central controller, wherein the at least imager system is operably coupled to the central controller. The central controller is configured to receive image data from an imager system, analyze the image data to identify at least one mark formed in a soil surface, and responsive to identifying the at least one mark, cause feedback regarding a current position and orientation of the implement and an ideal position and orientation of the implement to be output via an input/output device.

Abstract: The present invention is directed to antibodies and antigen binding fragments thereof having binding specificity for PACAP. The antibodies and antigen binding fragments thereof comprise the sequences of the VH, VL, and CDR polypeptides described herein, and the polynucleotides encoding them. Antibodies and antigen binding fragments described herein bind to and/or compete for binding to the same linear or conformational epitope(s) on human PACAP as an anti-PACAP antibody. The invention contemplates conjugates of anti-PACAP antibodies and binding fragments thereof conjugated to one or more functional or detectable moieties. Methods of making said anti-PACAP antibodies and antigen binding fragments thereof are also contemplated.

Abstract: A system for maintaining accurate health data and for providing rewards to data market participants. A marketplace platform based on Blockchain technology leverages smart contracts to provide rewards to sellers of data assets that provide corrections or updates to medical data, such as provider demographics. A URL API is provided to a buyer of the data with an encrypted password that is used to access the data from the URL API.

Abstract: An insert for an arthropod trapping device. The insert comprising a substrate and a frame for supporting the substrate, where a surface of the substrate has an adhesive disposed thereon, an optional mounting bracket spaced apart from the adhesive surface of the insert and located at a first end of the insert, and an optional graspable tab extending from the frame at a second end of the insert.

Abstract: Embodiments of the present invention are directed to an adjustable-incline climbing wall. The climbing wall comprises one or more climbing panels supported by a frame and a system for adjusting the incline of the frame to provide a climbing wall of a desired incline. The incline of the climbing wall may be adjusted by activating an actuator, which extends the upper portion of the frame a distance from a support wall, tilting the climbing wall to a desired incline. When the climbing wall is substantially vertically oriented, the system for adjusting the incline of the climbing wall may extend only a small distance from the support wall so that the adjustable-incline climbing wall has a relatively small footprint. In some embodiments, the climbing wall may also comprise one or more fitness accessories that can brought to an optimal height for a particular user through adjustment of the climbing wall incline.

Abstract: Disclosed are a device, a computer-readable medium, and techniques that provide an onboarding process and an adaptivity process for a drug delivery device. A processor executing an onboarding process determines whether a history of delivered insulin to a user meets certain sufficiency requirements. The onboarding process enables a processor to cause the drug delivery device to administer doses of insulin to a user according to an initial total daily insulin dose calculation that is determined based on the sufficiency of the insulin delivery history. The initial total daily insulin may be adapted according to the adaptivity process as new insulin delivery is collected. The insulin delivery history, when sufficient, may be used to set total daily insulin dosages that enable automated insulin delivery upon replacement of a drug delivery device. The adaptivity process may be implemented to modify an initial insulin delivery doses to provide adapted insulin delivery doses.

Abstract: Systems and methods of using a multiport assembly and associated components are disclosed. The multiport assembly can include a multiport device that communicatively couples multiple sets or pairs of connectors, such as optical connectors or electrical connectors, together. The multiport assembly can also include an auxiliary port device that couples to the multiport device to expand the capacity of the multiport assembly. Both the multiport and auxiliary port devices can be selectively configured to receive specific types of connectors by selectively coupling to different types of adapters, where the types of adapters correspond to the types of connectors. When coupled to the multiport or auxiliary port devices, the adapters facilitate the formation of the communication between its corresponding set of connectors.

Abstract: A method of preparing a high fat extruded protein product includes feeding water, a first fat, and a blend of plant proteins to an extruder to produce an extruded protein mixture. The extruded protein mixture is directed through a cooling die and divided into pieces, with the pieces being subsequently tumbled with oil. The resulting high fat extruded protein product will have at least 15-23% total fat by weight and, upon cooking, exhibits a desirable grease-out effect (e.g., reminiscent of a traditional pepperoni product). The blend of plant proteins preferably includes soy protein, wheat gluten and a grain or starch-based ingredient, and makes up at least 40-50%, by weight, of the high fat extruded protein product.

Abstract: Feeds in a network-based marketplace are described. The system receives a request, over a network, from a user that is associated with feed selection information, and identifies source feeds based on the feed selection information. The source feeds respectively include a first plurality of content elements. The system generates a presentation feed by retrieving a second plurality of content elements from the source feeds. The generating is performed continuously and in real-time. The system segments the presentation feed into pages and generates interfaces that include a first interface based on the pages. The system communicates the first interface, over the network, to the user, responsive to the receiving of the request.

Abstract: A device for performing autonomous agricultural operations may include a toolbar to which a plurality of implements may be interchangeably coupled, and a pair of parallel chassis beams mounted perpendicularly on the toolbar. At least a portion of each of the chassis beams may be telescopic and configured to be extended outward from, and retracted inward towards, the toolbar. The device may also include a plurality of drive assemblies each mounted on one of the chassis beams, and a plurality of motors corresponding to the drive assemblies and configured to drive the drive assemblies in accordance with one or more drive parameters to move the device throughout a site. The device may further include a computing device configured to automatically determine the drive parameters, and cause the plurality of motors to drive the corresponding drive assemblies in accordance with the drive parameters.

Abstract: A chip includes a first active region, first gates extending over the first active region in a first direction, wherein the first gates correspond to a first transistor, and second gates extending over the first active region in the first direction, wherein the second gates correspond to a second transistor.

Abstract: A method for producing a tandem sub for a perforating gun system includes positioning an electrically conductive signal bar in a central passage of a tubular housing of the tandem sub, and injecting a mold material into an annulus formed between an outer surface of the signal bar and an inner surface of the tubular housing that sealably adheres to both the signal bar and the tubular housing.