Abstract: A method for non-enzymatic 3D culture and amplification of mesenchymal stem cells (MSCs) includes the followings steps: preparing PLGA porous microspheres; preparing a PLGA-PEG-PLGA thermosensitive coating microcarrier; culturing and amplifying MSCs; and performing non-enzymatic separation of MSCs, including reducing a culture temperature to below a critical phase transition temperature, and centrifuging a culture medium to collect stem cells. The present invention adopts the method for non-enzymatic 3D culture and amplification of MSCs, wherein the PLGA porous microspheres are used as a cell culture microcarrier scaffold and the thermosensitive hydrogel PLGA-PEG-PLGA is coated on surfaces of such microspheres, without needing additional enzymolysis process, thus efficiently amplifying the stem cells.

Abstract: A probiotic microcapsule for the treatment of reproductive tract infection and a preparation method and application thereof are provided. The probiotic microcapsule is obtained by coating probiotics with the coating material. The probiotics include one or several of Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus crimpus, Lactobacillus brevis, Lactobacillus salivary, Lactobacillus plantarum, Lactobacillus acidophilus, and Lactobacillus bulgaricus. As probiotics screened in reproductive tract, it has high safety; It endows probiotics with adhesion function to ensure their effect in the reproductive tract; It overcomes the defects of current antibiotic therapy; It is expected to be used in a variety of reproductive tract infectious diseases.

Abstract: A transdermal double-layer microneedle based on hydrogen producing probiotics and its preparation method and application thereof are provided. The transdermal double-layer microneedle consists of a needle body and a backing layer. The raw material of the needle body includes hyaluronic acid and hyaluronidase. The raw material of the backing layer includes methacrylate anhydride gelatin and silk fibroin protein. The hydrogen producing bacteria are loaded in the microneedles, and the pore size of the matrix material is lower than the diameter of the probiotics, which will not lead to the leakage of bacteria and has high safety. Treatment of inflammatory skin diseases by gas has low side effects. The needle tip is loaded with hyaluronidase to increase the tissue space and improve the effect of gas treatment. It is expected to be used in psoriasis and other inflammatory skin diseases.

Abstract: Disclosed is a high anticoagulation extracorporeal circulation tube, which include the following preparation methods: S1, firstly, aminating the surface of the tube by extrusion molding; S2, activating heparin groups by a direct coupling method; S3, heparinizing PVC or PC tubes; S4, then, using albumin, functionalized PEG which can react with amino groups, and phosphate to further surface modify the inner wall of heparinized PVC or PC tube to block the sites not modified by heparinization, so as to shield the adsorption of platelets and protein in blood on the inner wall. The application can produce a novel high anticoagulation extracorporeal circulation tube with low price and high biocompatibility, which expands the application in clinic.

Abstract: Disclosed is a high anticoagulation ECMO and extracorporeal circulation consumable, which include the following preparation methods: S1, aminating the surface of ECMO blood circulation device and extracorporeal circulation consumables; S2, activating heparin groups; S3, heparinizing the ECMO blood circulation device and extracorporeal circulation consumables; S4, modification of enhancer. The application can produce a novel high anticoagulation extracorporeal circulation tube with low price and high biocompatibility, which expands the application in clinic.

Abstract: Disclosed is a high anticoagulation extracorporeal circulation tube, which include the following preparation methods: S1, firstly, aminating the surface of the tube by extrusion molding; S2, activating heparin groups by a direct coupling method; S3, heparinizing PVC or PC tubes; S4, then, using albumin, functionalized PEG which can react with amino groups, and phosphate to further surface modify the inner wall of heparinized PVC or PC tube to block the sites not modified by heparinization, so as to shield the adsorption of platelets and protein in blood on the inner wall. The application can produce a novel high anticoagulation extracorporeal circulation tube with low price and high biocompatibility, which expands the application in clinic.

Abstract: The present application generally relates to several processes for the preparation of (6R,10S)-10-{4-[5-chloro-2-(4-chloro-1H-1,2,3-triazol-1-yl)phenyl]-6-oxo-1(6H)-pyrimidinyl}-1-(difluoromethyl)-6-methyl-1,4,7,8,9,10-hexahydro-11,15-(metheno)pyrazolo[4,3-b][1,7]diazacyclotetradecin-5(6H)-one: Compound (I).

Abstract: For communicably connecting with an opposing apparatus using VLANs on multiple communication pathways, communication addresses of the opposing apparatus and relay apparatuses located on the communication pathways are acquired. Used VLAN-IDs already being utilized for configuring other VLANs by the opposing apparatus and the relay apparatuses located on the communication pathways are acquired. A VLAN-ID that is not being utilized is identified based on the used VLAN-IDs that are already being utilized for configuring other VLANs by a local apparatus, the relay apparatuses, and the opposing apparatus. The identified VLAN-ID is used to set settings for communicably connecting the opposing apparatus and the relay apparatuses on a single communication pathway using a new VLAN.

Abstract: Provided herein are methods for treating cancer and for selecting a cancer treatment based on the expression of Stag2/3 proteins and/or the presence of mutations in the genes encoding Stag2/3 proteins.

Abstract: The present invention discloses a nano-armored single cell product, comprising a liposome and probiotics encapsulated by the liposome, wherein the probiotics are fermented to produce gamma-aminobutyric acid (GABA) that alleviates the activation of an inflammatory response in substantia nigra inducedin a MPTP induced PD model, thus mitigating an inflammatory injury to dopaminergic neurons in substantia nigra and having a neuroprotective effect; encapsulation of the probiotics by the liposome can protect the probiotics from strong acids and digestive enzymes in gastric acid.

Abstract: The present invention discloses a nano-armored single cell product, comprising a liposome and probiotics encapsulated by the liposome, wherein the probiotics are fermented to produce gamma-aminobutyric acid (GABA) that alleviates the activation of an inflammatory response in substantia nigra inducedin a MPTP induced PD model, thus mitigating an inflammatory injury to dopaminergic neurons in substantia nigra and having a neuroprotective effect; encapsulation of the probiotics by the liposome can protect the probiotics from strong acids and digestive enzymes in gastric acid.

Abstract: A method for non-enzymatic 3D culture and amplification of mesenchymal stem cells (MSCs) includes the followings steps: preparing PLGA porous microspheres; preparing a PLGA-PEG-PLGA thermosensitive coating microcarrier; culturing and amplifying MSCs; and performing non-enzymatic separation of MSCs, including reducing a culture temperature to below a critical phase transition temperature, and centrifuging a culture medium to collect stem cells. The present invention adopts the method for non-enzymatic 3D culture and amplification of MSCs, wherein the PLGA porous microspheres are used as a cell culture microcarrier scaffold and the thermosensitive hydrogel PLGA-PEG-PLGA is coated on surfaces of such microspheres, without needing additional enzymolysis process, thus efficiently amplifying the stem cells.

Abstract: Embodiments provide a seat connecting device with an adjustable backrest, belonging to the technical field of swivel chairs. In the embodiments, the seat connecting device includes a seat back tightness control assembly including a control handle, a universal joint, a transmission rod, a first bevel gear, a second bevel gear, a lifting part, a mounting seat of the lifting part, and a tightness adjust rod.

Abstract: A recombinant subunit vaccine microneedle product includes a backing and a recombinant subunit lung-associated virus vaccine solution-containing microneedle array attached to a side of the backing, wherein the recombinant subunit lung-associated virus vaccine solution-containing microneedle array includes a plurality of microneedles, wherein each microneedle contains a matrix and a recombinant subunit loaded in the matrix. The present invention adopts the recombinant subunit vaccine microneedle product as well as a preparation method and an application thereof, wherein such microneedle product can realize a rapid humoral immunity after being administered to the skins of animal bodies (especially human bodies), which solves the problems of traditional lung-associated virus vaccine injection, such as muscular pain and multiple injections.

Abstract: An mRNA vaccine microneedle product includes a backing and a mRNA vaccine solution-containing microneedle array attached to a side of the backing, wherein the mRNA solution-containing microneedle array includes several microneedles, and each microneedle contains a matrix and mRNA loaded in the matrix. The present invention adopts the mRNA vaccine microneedle product as well as a preparation method and an application thereof, and such microneedle product can realize a rapid humoral immunity after being administered to the skins of animal bodies (especially human bodies), which solves the problems of traditional vaccination, such as muscular pain and multiple injections.

Abstract: An inactivated virus vaccine microneedle product includes a backing and an inactivated virus-containing microneedle array attached to a side of the backing, wherein the inactivated virus-containing microneedle array includes a plurality of microneedles, and each microneedle contains a matrix and an inactivated virus loaded in the matrix. The present invention adopts the inactivated virus vaccine microneedle product as well as a preparation method and an application thereof, and such microneedle product realizes efficient transdermal absorption of a vaccine by loading an inactivated virus in the microneedle product after being administered to the skin, and a long-acting stable release of a vaccine is achieved, which solves the problems of traditional vaccination, such as muscular pain and multiple injections.