Abstract: This invention relates to methods and kits for typing and assembling discontinuous genomic elements.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures, without use of a support bath, and the like. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed (e.g., continuously printed using a moving dispensing nozzle) in air without the use of supports (e.g., without the use of a support bath, a concurrently printed support posts, or the like) while a nebulized coagulation agent is dispersed alongside the printed polymer solution to at least partially coagulate the polymer solution and form an intermediate article. The self-supporting intermediate article may then be immersed in a post-printing coagulation solution to remove some or all of the remaining solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: Described are systems. apparatuses. and methods for three-dimensional (3D) printing engineered parts having spatially tunable porosity. Printing ink can include metal/ceramic powders, binder(s), sinterable additive(s), polymer(s), porogen(s), and/or solvent(s). Vapor-based phase separation of the printing material causes at least partial solidification of ink to form a green part. A coagulation bath can be used to complete solidification of the green part and remove porogen material(s). Debinding the green part removes polymer(s) and/or porogen material(s) to form a porous, sinterable part. Sintering densifies the debinded part, controls grain growth/size, and improves mechanical properties of the part. Tuning concentration and particle size of porogen in the ink and/or the inter-filament spacing during printing achieves desired porosity and pore size in the finished part.

Abstract: This disclosure provides a novel method and system using a “spatial genome aligner” that parses true chromatin signals from noise by aligning signals to a DNA polymer model. This spatial genome aligner can efficiently reconstruct chromosome architectures from DNA-fluorescence in situ hybridization (DNA-FISH) data across multiple scales and determine chromosome ploidies de novo in interphase cells.

Abstract: The present invention relates to methods for haplotype determination and, in particular, haplotype determination at the whole genome level as well as targeted haplotype determination.

Abstract: The present invention relates to methods for haplotype determination and, in particular, haplotype determination at the whole genome level as well as targeted haplotype determination.

Abstract: A method and device for training a model based on federated learning are provided.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures, without use of a support bath, and the like. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed (e.g., continuously printed using a moving dispensing nozzle) in air without the use of supports (e.g., without the use of a support bath, a concurrently printed support posts, or the like) while a nebulized coagulation agent is dispersed alongside the printed polymer solution to at least partially coagulate the polymer solution and form an intermediate article. The self-supporting intermediate article may then be immersed in a post-printing coagulation solution to remove some or all of the remaining solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: Methods and kits for genome-wide identification of chromatin interactions in a cell are provided.

Abstract: A three-dimensional (3D) printing method and apparatus are disclosed for freeform fabrication of metal articles. 3D printed articles are formed from a build material comprising metal powder(s), polymer(s), and solvent(s). A coagulation agent, such as a nebulized non-solvent, is disposed onto/about the build material during 3D printing to cause at least partial solidification of the build material to form a green body structure. Multiple build materials can be mixed at a variable ratio to achieve a composition gradient through the green body structure. The 3D printed green body structure can be heated to remove some or all of the polymer, solvent, and/or for debinding. The debinded green body structure can be sintered at a specific sintering temperature or over a temperature gradient, for a period of time, in accordance with the sintering properties of the particular metal powder in the debinded green body structure, to form a finished metal part.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures, without use of a support bath, and the like. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed (e.g., continuously printed using a moving dispensing nozzle) in air without the use of supports (e.g., without the use of a support bath, a concurrently printed support posts, or the like) while a nebulized coagulation agent is dispersed alongside the printed polymer solution to at least partially coagulate the polymer solution and form an intermediate article. The self-supporting intermediate article may then be immersed in a post-printing coagulation solution to remove some or all of the remaining solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: The present invention relates to methods for the joint analysis of regulation of gene expression and gene expression in single cells. Provided are methods for obtaining gene expression information for a single nucleus, the methods comprising deriving a DNA library from the genomic DNA in one or more nuclei and deriving an RNA library from the RNA in one or more nuclei, sequencing the molecules in the RNA library and the DNA library, and correlating the RNA library and the DNA library for each of the one or more nuclei.

Abstract: A three-dimensional (3D) printing method and apparatus are disclosed for freeform fabrication of metal articles. 3D printed articles are formed from a build material comprising metal powder(s), polymer(s), and solvent(s). A coagulation agent, such as a nebulized non-solvent, is disposed onto/about the build material during 3D printing to cause at least partial solidification of the build material to form a green body structure. Multiple build materials can be mixed at a variable ratio to achieve a composition gradient through the green body structure. The 3D printed green body structure can be heated to remove some or all of the polymer, solvent, and/or for debinding. The debinded green body structure can be sintered at a specific sintering temperature or over a temperature gradient, for a period of time, in accordance with the sintering properties of the particular metal powder in the debinded green body structure, to form a finished metal part.

Abstract: The present invention relates to methods for haplotype determination and, in particular, haplotype determination at the whole genome level as well as targeted haplotype determination.

Abstract: There are provided, inter alia, methods for treatment of cancer. The methods include means of identifying the NAD biosynthetic pathway on which depend cancer cells in a tumor, in a subject suffering from cancer. The methods further include administering to a subject in need a therapeutically effective amount of an inhibitor of the NMRK1 enzyme pathway to a tumor where the cancer cells are dependent of the NAD salvage pathway, allowing to lower the of dose FK866 administered to the subject. The methods also include administering to a subject in need a therapeutically effective amount of a bacterial inhibitor of NADSYN1 to a tumor where the cancer cells depend on the Preiss Handler pathway.

Abstract: Provided herein are, inter alia, methods and compositions to detect, monitor and treat cancer, wherein the cancer includes amplified extrachromosomal oncogenes. The methods are useful for personalized treatment and exploit differential expression and chromatin structure of extrachromosomal oncogenes in cancer cells.

Abstract: The present invention relates to methods for haplotype determination and, in particular, haplotype determination at the whole genome level as well as targeted haplotype determination.

Abstract: A polymer three-dimensional (3D) printing methodology is disclosed for freeform fabrication of polymeric structures under ambient conditions without the use of printed support structures, without use of a support bath, and the like. The build material can be dissolved in a suitable solvent for 3D printing. The polymer solution can be printed (e.g., continuously printed using a moving dispensing nozzle) in air without the use of supports (e.g., without the use of a support bath, a concurrently printed support posts, or the like) while a nebulized coagulation agent is dispersed alongside the printed polymer solution to at least partially coagulate the polymer solution and form an intermediate article. The self-supporting intermediate article may then be immersed in a post-printing coagulation solution to remove some or all of the remaining solvent, causing the build material to fully solidify to form a finished article from the intermediate article.

Abstract: A method and device for training a model based on federated learning are provided.

Abstract: A method and apparatus applies an action to a software application by determining a target object for the input action. The determination of the target object is performed by identifying the target object through socially identifying object information relative to a reference object. Then, the input action is applied to the target object.