Patents by Inventor Bonnie L. Bassler

Bonnie L. Bassler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220010284
    Abstract: Disclosed are recombinant phages that infect and kill bacterial hosts in response to user-defined inputs. The components that encode the user-defined inputs can be combined, such that multiple inputs are maintained on a single recombinant phage, enabling precise control over the targeting strategy. The phages can be engineered to kill a specific bacterial species or multiple species simultaneously. Recombinant phages can also be engineered to harbor fluorescent and bioluminescent reporter genes that enable them to be used for tracking, detection, and in biosensing applications. Recombinant phages can also be used to lyse bacterial cells that produce recombinant proteins, as a rapid method to enable extraction and high-level purification of potentially valuable and/or industrially important proteins.
    Type: Application
    Filed: October 31, 2019
    Publication date: January 13, 2022
    Applicant: The Trustees of Princeton University
    Inventors: Justin E. Silpe, Bonnie L. Bassler
  • Publication number: 20210186010
    Abstract: The invention relates to compositions comprising Quorum-Sensing (QS) modulating molecules attached to a surface via a linker. This QS modulator attached surface can then be used to modulate QS, biofilm production, biofilm streamer production and/or virulence factor production. The length of the linker that attaches the QS modulating molecule to the surface as well as the surface coverage density impact QS modulation on surfaces. These QS modulator attached surfaces can be used to treat areas known to contain human pathogens notorious for causing hospital-acquired infections as well as fatal infections that occur outside of health care settings. Other surfaces that can be coated according to embodiments of the invention include abiotic materials, such as intravenous catheters, implants, medical devices, and cooling towers. Preferred microorganisms that can be treated with the compositions of the invention include, but are not limited to S. aureus and/or P. aeruginosa.
    Type: Application
    Filed: October 17, 2020
    Publication date: June 24, 2021
    Applicant: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Howard A. Stone, Min Young Kim, Thomas William Muir, Aishan Zhao
  • Publication number: 20190307818
    Abstract: The invention relates to compositions for inhibiting or agonizing quorum sensing (QS) pathways to repress biofilm formation, biofilm streamer formation, virulence factor production, and/or infections (including bacterial infections and bacterial infections with resistance to antibiotics) by: (a) a monotherapy using novel compounds, such as small molecule inhibitors or agonists, to inhibit or activate QS activation pathways and/or (b) a combination therapy including novel compounds to inhibit or activate QS and/or to sensitize bacteria to phage along with phage therapy. These compositions can be used to treat patients having infections. Additionally, these compositions can be used to treat surfaces/areas known to contain human pathogens notorious for causing hospital-acquired infections (such as intravenous catheters, implants, medical devices) as well as fatal infections that occur outside of health care settings.
    Type: Application
    Filed: April 7, 2017
    Publication date: October 10, 2019
    Applicant: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Nina M. Hoyland-Kroghsbo, Jon E. Paczkowski
  • Patent number: 10285978
    Abstract: A structurally distinct and potent series of synthetic small molecule activators of Vibrio cholerae quorum sensing have been chemically synthesized. The small molecule activators reduce virulence in V. cholerae. Acyl pyrrole molecules displayed strong potency and stability, particularly 1-(1H-pyrrol-3-yl)decan-1-one.
    Type: Grant
    Filed: November 29, 2017
    Date of Patent: May 14, 2019
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Lark J. Perez, Martin F. Semmelhack
  • Publication number: 20180346525
    Abstract: According to present invention embodiments, a trackable moiety can be attached to a quorum sensing (QS) molecule to form a QS modulating conjugate. QS modulating conjugates retain their activity for QS manipulation and are able to be detected by imaging techniques. The QS portion of the QS modulating conjugate can play a role in affecting bacterial behaviors, such as, inhibition of biofilms or disruption of toxin production, while the trackable moiety of the QS modulating conjugate enables monitoring, visualization in real time of its binding to the receptor on the bacterial surface, and the location of the bacterium itself, for example, in a biofilm and/or at an infection site. Since binding of the QS modulating conjugate to its cognate receptor is specific, the QS modulating conjugate can be used for diagnostic applications by enabling pinpointing of specific bacteria at infection sites.
    Type: Application
    Filed: April 11, 2018
    Publication date: December 6, 2018
    Applicant: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Howard A. Stone, Min Young Kim, Aishan Zhao, Thomas William Muir
  • Patent number: 9968587
    Abstract: A structurally distinct and potent series of synthetic small molecule activators of Vibrio cholerae quorum sensing have been chemically synthesized. The small molecule activators reduce virulence in V. cholerae. Acyl pyrrole molecules displayed strong potency and stability, particularly 1-(1H-pyrrol-3-yl)decan-1-one.
    Type: Grant
    Filed: August 19, 2014
    Date of Patent: May 15, 2018
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Lark J. Perez, Martin F. Semmelhack
  • Publication number: 20180125066
    Abstract: The invention relates to compositions comprising QS modulating molecules attached to a surface via a linker. This QS modulator attached surface can then be used to modulate QS, biofilm production, biofilm streamer production and/or virulence factor production. The length of the linker that attaches the QS modulating molecule to the surface as well as the surface coverage density are features that impact QS modulation on surfaces. These QS modulator attached surfaces can be used to treat areas known to contain human pathogens notorious for causing hospital-acquired infections as well as fatal infections that occur outside of health care settings. Other surfaces that can be coated according to embodiments of the invention include abiotic materials, such as intravenous catheters, implants, medical devices, and cooling towers. Preferred microorganisms that can be treated with the compositions of the invention include, but are not limited to S. aureus and/or P. aeruginosa.
    Type: Application
    Filed: October 25, 2017
    Publication date: May 10, 2018
    Applicant: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Howard A. Stone, Min Young Kim, Thomas William Muir, Aishan Zhao
  • Publication number: 20180078526
    Abstract: A structurally distinct and potent series of synthetic small molecule activators of Vibrio cholerae quorum sensing have been chemically synthesized. The small molecule activators reduce virulence in V. cholerae. Acyl pyrrole molecules displayed strong potency and stability, particularly 1-(1H-pyrrol-3-yl)decan-1-one.
    Type: Application
    Filed: November 29, 2017
    Publication date: March 22, 2018
    Applicant: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Lark J. Perez, Martin F. Semmelhack
  • Patent number: 9751851
    Abstract: Antivirulence strategies to combat Pseudomonas aeruginosa, are described. One strategy encompasses synthesis of a series of compounds that inhibit the production of pyocyanin, a redox-active virulence factor produced by this pathogen. A related strategy encompasses synthesis of compounds that inhibit the two P. aeruginosa quorum-sensing receptors, LasR and RhlR, inhibit production of pyocyanin, and inhibit biofilm formation.
    Type: Grant
    Filed: September 19, 2014
    Date of Patent: September 5, 2017
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Martin F. Semmelhack, Knut Drescher, Albert Siryaporn, Laura C. Conrad-Miller, Colleen T. O'Loughlin
  • Patent number: 9752175
    Abstract: Methods and systems of measuring biofilms and/or biofilm streamers are presented. The system has at least one channel, a biofilm streamer promotion element, a fluid capable of moving along the channel, wherein the fluid has a flow driven by a controlled pressure; and a measuring element capable of measuring a flow rate of the fluid through the channel. Additionally, methods of screening for compound(s) that promote and/or inhibit biofilm and/or biofilm streamers using this system are also presented. Uses of the system, methods and identified compounds are presented for medical and/or industrial environments.
    Type: Grant
    Filed: January 5, 2015
    Date of Patent: September 5, 2017
    Assignee: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Howard A. Stone, Knut Drescher, Yi Shen
  • Patent number: 9573908
    Abstract: Using a whole-cell high-throughput screen, eleven molecules were identified that activate V. cholerae quorum sensing (QS). Eight molecules are receptor agonists and three molecules are antagonists of LuxO, the central NtrC-type response regulator that controls the global V. cholerae QS cascade. Pro-QS molecules are used for the development of novel anti-infectives.
    Type: Grant
    Filed: February 20, 2013
    Date of Patent: February 21, 2017
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Wai-Leung Ng, Lark J. Perez, Jianping Cong, Martin F. Semmelhack
  • Publication number: 20160368892
    Abstract: Antivirulence strategies to combat Pseudomonas aeruginosa, are described. One strategy encompasses synthesis of a series of compounds that inhibit the production of pyocyanin, a redox-active virulence factor produced by this pathogen. A related strategy encompasses synthesis of compounds that inhibit the two P. aeruginosa quorum-sensing receptors, LasR and RhlR, inhibit production of pyocyanin, and inhibit biofilm formation.
    Type: Application
    Filed: September 19, 2014
    Publication date: December 22, 2016
    Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Martin F. Semmelhack, Knut Drescher, Albert Siryaporn, Laura C. Conrad-Miller, Colleen T. O'Loughlin
  • Patent number: 9468624
    Abstract: A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
    Type: Grant
    Filed: June 15, 2015
    Date of Patent: October 18, 2016
    Assignee: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Lee R. Swem, Scott M. Ulrich, Colleen T. O'Loughlin
  • Publication number: 20150306067
    Abstract: A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
    Type: Application
    Filed: June 15, 2015
    Publication date: October 29, 2015
    Applicant: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Lee R. Swem, Scott M. Ulrich, Colleen T. O'Loughlin
  • Patent number: 9084773
    Abstract: A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
    Type: Grant
    Filed: May 28, 2014
    Date of Patent: July 21, 2015
    Assignee: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Lee R. Swem, Scott M. Ulrich, Colleen T. O'Loughlin
  • Publication number: 20150191765
    Abstract: The present invention relates to a method of measuring biofilms and/or biofilm streamers, wherein the method comprises a system that has at least one channel and a biofilm streamer promotion element, a fluid capable of moving along the channel, wherein the fluid has a flow driven by a controlled pressure; and a measuring element capable of measuring a flow rate of the fluid through the channel. Additionally, method of screening for compound(s) that promote and/or inhibit biofilm and/or biofilm streamers using this system are also contemplated. Uses of the system, methods and identified compounds are contemplated for medical and/or industrial environments.
    Type: Application
    Filed: January 5, 2015
    Publication date: July 9, 2015
    Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Howard A. Stone, Martin F. Semmelhack, Knut Drescher, Yi Shen, Colleen T. O'Loughlin, Laura C. Miller
  • Publication number: 20150126474
    Abstract: Using a whole-cell high-throughput screen, eleven molecules were identified that activate V. cholerae quorum sensing (QS). Eight molecules are receptor agonists and three molecules are antagonists of LuxO, the central NtrC-type response regulator that controls the global V. cholerae QS cascade. Pro-QS molecules are used for the development of novel anti-infectives.
    Type: Application
    Filed: February 20, 2013
    Publication date: May 7, 2015
    Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Wai-Leung Ng, Lark J. Perez, Jianping Cong, Martin F. Semmelhack
  • Publication number: 20140275232
    Abstract: A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 18, 2014
    Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Bonnie L. Bassler, Lee R. Swem, Scott M. Ulrich, Colleen T. O'Loughlin
  • Patent number: 8772331
    Abstract: A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
    Type: Grant
    Filed: July 17, 2012
    Date of Patent: July 8, 2014
    Assignee: The Trustees of Princeton University
    Inventors: Bonnie L. Bassler, Lee R. Swem, Scott M. Ulrich, Colleen T. O'Loughlin
  • Publication number: 20140024707
    Abstract: A novel small molecule antagonizes two types of acyl homoserine lactone receptors: membrane-bound and cytoplasmic. A focused library of analogs and derivatives of the original antagonist was synthesized. Analog and derivative molecules harbor a range of activities. The novel small molecule and most potent antagonist protects the eukaryote Caenorhabditis elegans from quorum-sensing-mediated killing by the bacterial pathogen Chromobacterium violaceum. The saving of C. elegans demonstrates the use of these molecules as small molecule antimicrobials.
    Type: Application
    Filed: July 17, 2012
    Publication date: January 23, 2014
    Inventors: Bonnie L. Bassler, Lee R. Swem, Scott M. Ulrich, Colleen T. O'Loughlin