Abstract: Provided herein are methods for assessing a preparation of guide RNA molecules for quality, including for purity and identity.

Abstract: Provided herein are safe harbor loci and methods for identifying and using safe harbor loci to engineer cells to express transgenes. The safe harbor loci exhibit increased transgene knock-in efficiency and allow for increased, stable expression of transgenes in engineered cells. Guide ribonucleic acids (gRNAs) may be used for insertion of transgenes in the safe harbor loci.

Abstract: Provided herein are safe harbor loci and methods for identifying and using safe harbor loci to engineer cells to express transgenes. The safe harbor loci exhibit increased transgene knock-in efficiency and allow for increased, stable expression of transgenes in engineered cells. Guide ribonucleic acids (gRNAs) may be used for insertion of transgenes in the safe harbor loci.

Abstract: A system and method for endpoint-supplemented distributed financial computing, comprising a plurality of financial institutions, a plurality of varied endpoints, an endpoint software distributor, and a preliminary analysis system. The system performs algorithmic analysis of financial data in two parts, first a preliminary analysis on the preliminary analysis system, and then a second portion of analysis on an endpoint running software delivered by the endpoint software distributor. The financial analysis may be performed with a single or plurality of financial institutions supplying base financial data to be analyzed, and the system may involve automated algorithms such as regularly-scheduled datamining algorithms in certain implementations.

Abstract: Provided herein are safe harbor loci and methods for identifying and using safe harbor loci to engineer cells to express transgenes. The safe harbor loci exhibit increased transgene knock-in efficiency and allow for increased, stable expression of transgenes in engineered cells. Guide ribonucleic acids (gRNAs) may be used for insertion of transgenes in the safe harbor loci.

Abstract: Provided herein are safe harbor loci and methods for identifying and using safe harbor loci to engineer cells to express transgenes. The safe harbor loci exhibit increased transgene knock-in efficiency and allow for increased, stable expression of transgenes in engineered cells. Guide ribonucleic acids (gRNAs) may be used for insertion of transgenes in the safe harbor loci.

Abstract: Provided herein are safe harbor loci and methods for identifying and using safe harbor loci to engineer cells to express transgenes. The safe harbor loci exhibit increased transgene knock-in efficiency and allow for increased, stable expression of transgenes in engineered cells. Guide ribonucleic acids (gRNAs) may be used for insertion of transgenes in the safe harbor loci.

Abstract: Modified template switching oligonucleotides (TSOs), compositions containing modified TSOs, and methods for employing modified TSOs to synthesize cDNA from RNA templates, where the cDNA includes an adapter region at the 3? end, are provided. The modified TSOs include at least one 2?-fluoro-ribonucleotide in the 3? annealing region and provide for improved conversion of RNA into full-length cDNA, resulting in increased yield and complexity as compared to non-modified TSOs and thereby finding use in generating cDNA from samples having low RNA input.

Abstract: Provided herein are methods and compositions for labeling target nucleic acid molecules with molecular barcodes.

Abstract: Provided herein are methods and compositions for labeling target nucleic acid molecules with molecular barcodes.

Abstract: This invention pertains to the creation of a complex pool of adapters that contain complementary barcodes to be utilized in next generation sequencing library prep methods and methods of using barcoded adapters for next generation sequencing.