Patents by Inventor Brent Brower
Brent Brower has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11952578Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.Type: GrantFiled: December 26, 2022Date of Patent: April 9, 2024Assignee: Monsanto Technology LLCInventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
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Publication number: 20240110194Abstract: The present disclosure provides novel compositions and methods for collectively transforming or genetically modifying a population of distinct germplasm of different germplasms or having different genotypes. The compositions of the present disclosure may include a population of distinct germplasm, such as embryo explants, and a heterologous polynucleotide molecule, a ribonucleoprotein, or a site-specific nuclease. The methods of the present disclosure may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture or particle bombardment, bud induction, extended bud induction, and/or regeneration or development of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explants with a heterologous polynucleotide.Type: ApplicationFiled: October 6, 2023Publication date: April 4, 2024Inventors: Brent Brower-Toland, David Vincent Butruille, Edward J. Cargill, Yurong Chen, Megan Elizabeth Hassebrock, Thomas Ream, Jennifer Rinehart, Mary Ann Saltarikos, Michelle Folta Valentine
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Publication number: 20230235350Abstract: Provided are compositions and methods for altering TFL1 levels in soybean plants. Methods and compositions are also provided for altering the expression of genes related to the timing of terminal differentiation of stem tips through suppression, mutagenesis and/or editing of the TFL1 gene. Modified plant cells and plants having a suppression element or mutation reducing the expression or activity of a TFL1 gene are further provided comprising reduced TFL1 levels and improved characteristics, such as reduced plant height and increased lodging resistance.Type: ApplicationFiled: November 8, 2022Publication date: July 27, 2023Inventors: Brent Brower-Toland, Jeongwoon Kim, Keith Merrill, Linda Rymarquis, Thomas L. Slewinski, David R. Wooten, JR.
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Publication number: 20230212596Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.Type: ApplicationFiled: December 26, 2022Publication date: July 6, 2023Inventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
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Publication number: 20230159946Abstract: The present invention provides recombinant DNA constructs, vectors and molecules useful for attenuating and/or refining the expression of a florigenic FT gene or transgene using targeting sequences of small RNA molecules. Transgenic plants, plant cells and tissues, and plant parts comprising the recombinant constructs, vectors, and molecules are also provided. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant via suppression, relative to a control or wild type plant. Methods are further provided for introducing the recombinant DNA constructs, vectors, and molecules into a plant, and planting transgenic plants in the field including at higher densities. Transgenic plants of the present invention may provide greater yield potential than wild type or control plants.Type: ApplicationFiled: November 28, 2022Publication date: May 25, 2023Applicant: Monsanto Technology LLCInventors: Brent BROWER-TOLAND, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya Howell, Brad Mcdill, Dan Ovadya, Beth Savidge, Vijay Sharma
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Patent number: 11566254Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.Type: GrantFiled: October 15, 2021Date of Patent: January 31, 2023Assignee: Monsanto Technology LLCInventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
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Patent number: 11555201Abstract: The present invention provides recombinant DNA constructs, vectors and molecules useful for attenuating and/or refining the expression of a florigenic FT gene or transgene using targeting sequences of small RNA molecules. Transgenic plants, plant cells and tissues, and plant parts comprising the recombinant constructs, vectors, and molecules are also provided. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant via suppression, relative to a control or wild type plant. Methods are further provided for introducing the recombinant DNA constructs, vectors, and molecules into a plant, and planting transgenic plants in the field including at higher densities. Transgenic plants of the present invention may provide greater yield potential than wild type or control plants.Type: GrantFiled: October 18, 2017Date of Patent: January 17, 2023Assignee: Monsanto Technology LLCInventors: Brent Brower-Toland, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya Howell, Brad McDill, Dan Ovadya, Beth Savidge, Vijay Sharma
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Publication number: 20220135991Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.Type: ApplicationFiled: October 15, 2021Publication date: May 5, 2022Inventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
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Patent number: 11319983Abstract: An arrangement configured to introduce a force from a fastening element into a plastic component, the arrangement including a force-limiting element that is embedded in the plastic component; wherein the fastening element is configured to fasten the plastic component at a body; wherein the fastening element is insertable through the force-limiting element, and wherein the plastic component includes a retention device which protrudes at least partially into an interior of the force-limiting element so that the fastening element that is inserted into the force-limiting element is retained in the force limiting element.Type: GrantFiled: December 14, 2019Date of Patent: May 3, 2022Assignee: ECO Holding 1 GmbHInventors: Daniel Stanhope, Brent Brower, Matt Carlson, Matt Stolzman
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Publication number: 20220090105Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.Type: ApplicationFiled: December 7, 2021Publication date: March 24, 2022Applicant: Monsanto Technology LLCInventors: Brent Brower-Toland, Rico A. Caldo, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya D. Howell, Balasulojini Karunanandaa, Sivalinganna Manjunath, Bradley W. McDill, Daniel J. Ovadya, Sasha Preuss, Elena A. Rice, Beth Savidge, Vijay K. Sharma
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Patent number: 11225671Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.Type: GrantFiled: March 29, 2019Date of Patent: January 18, 2022Assignee: Monsanto Technology LLCInventors: Brent Brower-Toland, Rico A. Caldo, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya D. Howell, Balasulojini Karunanandaa, Sivalinganna Manjunath, Bradley W. McDill, Daniel J. Ovadya, Sasha Preuss, Elena A. Rice, Beth Savidge, Vijay K. Sharma
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Patent number: 11186843Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.Type: GrantFiled: February 27, 2015Date of Patent: November 30, 2021Assignee: Monsanto Technology LLCInventors: Brent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
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Publication number: 20210180641Abstract: An arrangement configured to introduce a force from a fastening element into a plastic component, the arrangement including a force-limiting element that is embedded in the plastic component; wherein the fastening element is configured to fasten the plastic component at a body; wherein the fastening element is insertable through the force-limiting element, and wherein the plastic component includes a retention device which protrudes at least partially into an interior of the force-limiting element so that the fastening element that is inserted into the force-limiting element is retained in the force limiting element.Type: ApplicationFiled: December 14, 2019Publication date: June 17, 2021Inventors: Brent Brower, Daniel Stanhope, Matt Carlson, Matt Stolzman
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Publication number: 20210172345Abstract: An actuator for a cam phaser, the cam phaser including a hydraulic valve that is adjustable by the actuator, wherein the actuator is receivable at a housing section of a component that receives the cam phaser, wherein the actuator includes a retaining element and a housing with functionally relevant components, wherein the actuator is attached by the retaining element at the housing section, wherein the retaining element is configured separate from the housing, wherein safe positioning of the actuator at the housing section is provided by a section of the retaining element that supports the actuator at the housing section in a direction of an axial orientation of the actuator, and wherein a housing cover of the housing is at least partially arranged between the section of the retaining element and the housing section.Type: ApplicationFiled: February 4, 2021Publication date: June 10, 2021Inventors: Daniel Stanhope, Brent Brower
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Patent number: 10895178Abstract: An actuator for a cam phaser, wherein the cam phaser includes a hydraulic valve that is adjustable by the actuator, wherein the actuator is receivable at a housing section by at least one form locking connection, wherein the at least one form locking connection includes a first form element pair and a second form element pair, wherein the first form element pair includes a first stop and the second form element pair includes a second stop, and wherein the first stop and the second stop are oriented in opposite directions of rotation of the actuator.Type: GrantFiled: February 9, 2018Date of Patent: January 19, 2021Assignee: ECO Holding 1 GmbHInventors: Tim Wells, Brian Kujawski, Brent Brower
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Publication number: 20200362360Abstract: This disclosure provides recombinant DNA constructs and modified or transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency, and enhanced drought tolerance or water use efficiency. Modified or transgenic plants may include field crops as well as plant propagules, plant parts and progeny of such modified or transgenic plants. Methods of making and using such modified or transgenic plants are also provided, as are methods of producing seed from such modified or transgenic plants, growing such seed, and selecting progeny plants with enhanced traits. Further disclosed are modified or transgenic plants with altered phenotypes or traits which are useful for screening and selecting transgenic events, edits or mutations with a desired enhanced trait.Type: ApplicationFiled: November 21, 2018Publication date: November 19, 2020Inventors: Robert M. Alba, Edwards M. Allen, Brent Brower-Toland, Molian Deng, Todd DeZwaan, Charles Dietrich, Alexander Goldshmidt, Cara L. Griffith, Miya D. Howell, Niranjani J. Iyer, Hongwu Jia, Saritha V. Kuriakose, Hong Li, Linda L. Lutfiyya, Anil Neelam, Shengzhi Pang, Mingsheng Peng, Monnanda Somaiah Rajani, Daniel Ruzicka, Daniel P. Schachtman, Vijay K. Sharma, Tyamagondlu V. Venkatesh, Huai Wang, Xiaoyun Wu, Nanfei Xu
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Publication number: 20200024610Abstract: The present disclosure provides methods and compositions for identification of optimal genomic loci in plant genome for site-directed integration in plants.Type: ApplicationFiled: September 29, 2017Publication date: January 23, 2020Applicant: MONSANTO TECHNOLOGY LLCInventors: Brent BROWER-TOLAND, Paul S. CHOMET, Robert T. GAETA, Andrei Y. KOURANOV, Jonathan C. LAMB, Richard J. LAWRENCE, Ruth WAGNER
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Publication number: 20190300890Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.Type: ApplicationFiled: March 29, 2019Publication date: October 3, 2019Applicant: Monsanto Technology LLCInventors: Brent Brower-Toland, Rico A. Caldo, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya D. Howell, Balasulojini Karunanandaa, Sivalinganna Manjunath, Bradley W. McDill, Daniel J. Ovadya, Sasha Preuss, Elena A. Rice, Beth Savidge, Vijay K. Sharma
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Publication number: 20190218563Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.Type: ApplicationFiled: March 29, 2019Publication date: July 18, 2019Applicant: Monsanto Technology LLCInventors: Brent Brower-Toland, Rico A. Caldo, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya D. Howell, Balasulojini Karunanandaa, Sivalinganna Manjunath, Bradley W. McDill, Daniel J. Ovadya, Sasha Preuss, Elena A. Rice, Beth Savidge, Vijay K. Sharma
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Patent number: 10294486Abstract: The present invention provides recombinant DNA constructs, vectors and molecules comprising a polynucleotide sequence encoding a florigenic FT protein operably linked to a vegetative stage promoter, which may also be a meristem-preferred or meristem-specific promoter. Transgenic plants, plant cells and tissues, and plant parts are further provided comprising a polynucleotide sequence encoding a florigenic FT protein. Transgenic plants comprising a florigenic FT transgene may produce more bolls, siliques, fruits, nuts, or pods per node on the transgenic plant, particularly on the main stem of the plant, relative to a control or wild type plant. Methods are further provided for introducing a florigenic FT transgene into a plant, and planting transgenic FT plants in the field including at higher densities. Transgenic plants of the present invention may thus provide greater yield potential than wild type plants and may be planted at a higher density due to their altered plant architecture.Type: GrantFiled: April 18, 2016Date of Patent: May 21, 2019Assignee: Monsanto Technology LLCInventors: Brent Brower-Toland, Rico A. Caldo, Shunhong Dai, Karen Gabbert, Alexander Goldshmidt, Miya D. Howell, Balasulojini Karunanandaa, Sivalinganna Manjunath, Bradley W. McDill, Daniel J. Ovadya, Sasha Preuss, Elena A. Rice, Beth Savidge, Vijay K. Sharma