Abstract: Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.

Abstract: A peer to peer communication system and method are provided to enable interfacing with an application running on a gaming engine for an avatar simulation or video conference. The system and method establish a real-time peer-to-peer communication link between remotely located users for transmission in real-time of audio, video, and data communications. The system and method capture incoming audio and video transmissions from input devices operable by the users while controlling one or more avatars, and transmit, in real time, synchronized audio, video, and data communications to the users over the communication link.

Abstract: Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Abstract: Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for facilitating the detection of targets located proximally to each other in a sample.

Abstract: Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.

Abstract: A method for dispensing a beverage container to a consumer. The method includes receiving in a beverage container dispenser a first beverage container that contains a beverage corresponding to a beverage selection of a user. The method further includes dispensing a second beverage container containing the beverage from a temperature-regulated compartment of the beverage container dispenser. The second beverage container that is dispensed from the temperature-regulated compartment corresponds to the beverage selection of the user.

Abstract: Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Abstract: Methods and systems for predictive measures of anti-EGFR therapy response in wild type RAS/EGFR+ samples, e.g., histochemical staining methods for staining EGFR, AREG, and EREG, digital analysis of stained slides, and scoring algorithms that allow prediction of a response to anti-EGFR therapies. Analysis of the stained slides and scoring algorithms may include but are not limited to: a percent tumor cell positivity, computerized clustering algorithms, area density (e.g., area of tumor positive for one or more markers over total tumor area), average intensity (e.g., computerized methodology measuring average gray scale pixel intensity), average intensity broken down according to membrane, cytoplasmic, or punctate staining patterns), or any other appropriate parameter or combination of parameters. The methods of the present invention allow for resolving spatial expression patterns of the ligands and the receptor to determine what patterns are predictive for response to anti-EGFR therapies.

Abstract: A method for dispensing a beverage container to a consumer. The method includes receiving in a beverage container dispenser a first beverage container that contains a beverage corresponding to a beverage selection of a user. The method further includes dispensing a second beverage container containing the beverage from a temperature-regulated compartment of the beverage container dispenser. The second beverage container that is dispensed from the temperature-regulated compartment corresponds to the beverage selection of the user.

Abstract: A method for dispensing a beverage container to a consumer. The method includes receiving in a beverage container dispenser a first beverage container that contains a beverage corresponding to a beverage selection of a user. The method further includes dispensing a second beverage container containing the beverage from a temperature-regulated compartment of the beverage container dispenser. The second beverage container that is dispensed from the temperature-regulated compartment corresponds to the beverage selection of the user.

Abstract: Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Abstract: Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

Abstract: Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.

Abstract: Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.

Abstract: Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

Abstract: Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes “click chemistry” to covalently bind reporter molecules to tissue.

Abstract: Aspects of systems and methods for collaborating physical-virtual interfaces are disclosed. In an example, a method may include transmitting digital content to display in a virtual environment that includes one or more human inhabited characters, the digital content corresponding to content displayed on one or more interactive devices. The method may also include receiving first input data representing content markup of the digital content from a first user of the first interactive device. The method may also include determining an action state or an appearance state for a human inhabited character in response to the first data. The method may also include transmitting the first input data to display in the virtual environment and transmitting the human inhabited character to display in the virtual environment according to the action state or the appearance state.

Abstract: A peer to peer communication system and method are provided to enable interfacing with an application running on a gaming engine for an avatar simulation or video conference. The system and method establish a real-time peer-to-peer communication link between remotely located users for transmission in real-time of audio, video, and data communications. The system and method capture incoming audio and video transmissions from input devices operable by the users while controlling one or more avatars, and transmit, in real time, synchronized audio, video, and data communications to the users over the communication link.

Abstract: Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Abstract: Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.