Abstract: Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Abstract: Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

Abstract: Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.

Abstract: Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

Abstract: Disclosed herein are multiplex methods for co-detecting a B cell marker, TP53 nucleic acid, and Chromosome 17 centromere DNA in a single sample. Samples stained for the B cell marker (e.g., CD79a protein), TP53 nucleic acid, and Chromosome 17 centromere DNA may allow for the identification of the subtype of chronic lymphocytic leukemia (CLL) with the 17p deletion. The methods feature staining the B cell marker (e.g., CD79a) a first distinct color, TP53 in a second distinct color, and chromosome 17 centromere DNA in a third distinct color. Further disclosed are nucleic acid probes specific for 19q12, INSR, ATM, DLEU2, TP53, and 13q12.

Abstract: A control system provides an interface for virtual characters, or avatars, during live avatar-human interactions. A human interactor can select facial expressions, poses, and behaviors of the virtual character using an input device mapped to menus on a display device.

Abstract: Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

Abstract: Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Abstract: Disclosed herein are methods for detecting the presence and/or amount of HER2 protein, HER2 nucleic acid (for example, HER2 genomic DNA), ER protein, and Chromosome 17 centromere DNA in a single sample. Samples stained for HER2 protein, HER2 DNA, ER protein, and Chromosome 17 DNA allow for the identification of various types of cancer cells, for example HER2 protein positive/ER protein positive/HER2 gene positive cells, HER2 protein positive/ER protein negative/HER2 gene positive cells, HER2 protein negative/ER protein positive/HER2 gene positive cells, and HER2 protein negative/ER protein negative/HER2 gene positive cells.

Abstract: Embodiments of a system, method, and kit for visualizing a nucleus are disclosed. A tissue sample is pretreated with a protease to permeabilize the nucleus, and then incubated with a nanoparticle/DNA-binding moiety conjugate. The DNA-binding moiety includes at least one DNA-binding molecule. The conjugate binds to DNA within the nucleus, and the nanoparticle is visualized, thereby visualizing the nucleus. Computer and image analysis techniques are used to evaluate nuclear features such as chromosomal distribution, ploidy, shape, size, texture features, and/or contextual features. The method may be used in combination with other multiplexed tests on the tissue sample, including fluorescence in situ hybridization. Kits for performing the method include a protease enzyme composition, a nanoparticle/DNA-binding moiety conjugate, and a reaction buffer.

Abstract: Methods and systems for providing an electrically dimmable combiner optics for head-up displays are disclosed. In one embodiment, a dimmable head-up display includes a display portion configured to provide head-up display information to an operator. Additionally, a dimmable portion is coupled to the display portion configured to change a transmissivity of the display portion from a first transmissivity level to a second transmissivity level.

Abstract: Methods and systems for providing an electrically dimmable combiner optics for head-up displays are disclosed. In one embodiment, a dimmable head-up display includes a display portion configured to provide head-up display information to an operator. Additionally, a dimmable portion is coupled to the display portion configured to change a transmissivity of the display portion from a first transmissivity level to a second transmissivity level.

Abstract: Systems and methods for controlling windows with variable light transmission are disclosed. In one embodiment, a system includes a sensor adapted to receive a light intensity, a user input device adapted to receive a user input value, a window having a variable transmissivity portion, and a control system. The control system is operatively coupled to the sensor, the user input device, and the window, and is adapted to receive the light intensity and user input value and to adjust a transmission level of the variable transmissivity portion of the window based on at least one of the light intensity and the user input value. The sensor includes a sensor adapted to receive a light intensity, a temperature, a humidity, or other environmental variables. In other embodiments, the system also includes a ventilation system, an air conditioning system, or a humidity control system.

Abstract: An airplane pitch guidance control law and display symbol for a Traffic alert and Collision Avoidance System (TCAS) is disclosed. The control law converts a TCAS computer-generated vertical air speed command into a pitch guidance command. The pitch guidance command controls the location of a symbol (51) on the pitch axis of an electronic attitude display (21) that guides a pilot toward a pitch attitude that will result in a vertical speed that eliminates a collision threat. The preferred symbol is a pitch axis leg (53) and a pair of outwardly diverging legs (55a, 55b). The pitch axis leg (53) defines the minimum climb or descent attitude required to achieve a safe climb or descent angle. The pitch axis leg (53) and the diverging legs (55a, 55b) combine to define a climb or descent attitude range to avoid. The distance between the pitch axis leg 53 and the boresight 39 of the airplane symbol of the display is controlled by a RA.sub.CMD signal produced by: subtractively combining the vertical speed command (V.

Abstract: A flight path angle symbol is positioned on an aircraft attitude display indicator as a function of either a measured flight path angle or a flight path angle commanded by the pilot. When the aircraft is operating within a selected airspeed, angle of attack, pitch attitude or load factor range, or the difference between measured flight path angle and commanded flight path angle is within a selected range, the symbol is positioned on the display as a function of commanded flight path angle. However when the aircraft is operating outside any of these ranges, the symbols is positioned on the display as a function of the measured flight path angle. To avoid a rapid jump in symbol position when transitioning between measured and commanded flight path angle displays, a phase lag filter is used to attenuate and lag the symbol positioning signal.

Abstract: A pitch limit symbol is generated at an aircraft attitude indicator which provides a pilot with display of a stall warning angle of attack margin which is referenced to an airplane symbol on the primary attitude indicator. The position of the pitch limit symbol relative to the aircraft symbol is a function of the difference in angle of attack between the current angle of attack at the aircraft, and a predetermined angle of attack at which the onset of stall warning occurs. Additional information is provided on the attitude indicator by a flight path angle symbol. The pitch limit symbol together with the flight path angle symbol provides the pilot with information to achieve a positive rate of climb during a pitch up maneuver while adjusting the pitch attitude of the aircraft to avoid a stall situation. The position of the pitch limit symbol may be compensated by various means to decrease the distance between the aircraft symbol and the pitch limit symbol.This is a continuation of application Ser. No.