Abstract: A vehicle or shipping-container monitoring system is provided. The system is used to sample the interior environment of the container and if needed, clear a potentially hazardous material from the interior of the container prior to conventional opening. The system includes a monitoring assembly affixed to a first through-penetration in the vehicle container, an evacuation assembly affixed to a second through-penetration in the vehicle container, and an intake penetration with a cap affixed to a third through-penetration in the vehicle container. A method is also provided to assess the interior of a vehicle or shipping container for hazardous gases or vapors prior to opening the container.

Abstract: A valve assembly for a fuel tank includes a cage having a seat that defines an aperture and a spring latch that holds a poppet in an intermediate-open position, which spaces the poppet from a seat to allow fluid communication through an aperture. A method of operating a valve assembly due to a first-time fueling event is disclosed.

Abstract: A valve assembly for a fuel tank includes a cage having a seat that defines an aperture and a spring latch that holds a poppet in an intermediate-open position, which spaces the poppet from a seat to allow fluid communication through an aperture. A method of operating a valve assembly due to a first-time fueling event is disclosed.

Abstract: Bioreactor systems can include a first frame and a second frame, a well plate, a motor plate, a motor, and a controller. The first frame may define a well plate inset and standoff insets for a first set of metal standoffs. The second frame may define a plurality of mounts and a plurality of insets for a second set of metal standoffs. A gear may be positioned in each of the plurality of mounts. A paddle may be coupled to each of the gears. The well plate can be positioned within the well plate inset. The motor plate can be supported by and connected to the first set of metal standoffs and the second set of metal standoffs. The motor can be mounted on the motor plate and operatively connected to one of the plurality of gears.

Abstract: Disclosed are methods of targeted sequencing by using gRNA-endonuclease complexes and methods of designing pools of gRNAs. The disclosure also provides sequencing adapters that that comprise a double-stranded nucleic acid having a single-stranded overhang with degenerate overhanging bases. In a first aspect, methods for Dephosphorylate targeted sequencing of double-stranded nucleic acids comprises cleaving dephosphorylated double-stranded nucleic acids with a plurality of endonuclease-guide ribonucleic acid (gRNA) complexes to generate double-stranded nucleic acid fragments having phosphorylated 5? end overhangs at targeted sites.

Abstract: Disclosed herein are, among other things, systems and methods for providing digital content for multiple venues to mobile devices. In some embodiments, venue location data defining a geographic boundary of each of a plurality of venues and venue content data for a plurality of different attractions associated with the venue is stored. A processing device system may be configured to determine a current location of the mobile device, and provide at least some of the venue content data based at least on an analysis of the venue location data, or attraction location data, and the current location.

Abstract: Disclosed herein are, among other things, systems and methods for providing digital content for multiple venues to mobile devices. In some embodiments, venue location data defining a geographic boundary of each of a plurality of venues and venue content data for a plurality of different attractions associated with the venue is stored. A processing device system may be configured to determine a current location of the mobile device, and provide at least some of the venue content data based at least on an analysis of the venue location data, or attraction location data, and the current location.

Abstract: Disclosed herein are, among other things, systems and methods for providing digital content for multiple venues to mobile devices. In some embodiments, venue location data defining a geographic boundary of each of a plurality of venues and venue content data for a plurality of different attractions associated with the venue is stored. A processing device system may be configured to determine a current location of the mobile device, and provide at least some of the venue content data based at least on an analysis of the venue location data, or attraction location data, and the current location.

Abstract: Disclosed herein are, among other things, systems and methods for providing digital content for multiple venues to mobile devices. In some embodiments, venue location data defining a geographic boundary of each of a plurality of venues and venue content data for a plurality of different attractions associated with the venue is stored. A processing device system may be configured to determine a current location of the mobile device, and provide at least some of the venue content data based at least on an analysis of the venue location data, or attraction location data, and the current location.

Abstract: Disclosed herein are, among other things, systems and methods for providing digital content for multiple venues to mobile devices. In some embodiments, venue location data defining a geographic boundary of each of a plurality of venues and venue content data for a plurality of different attractions associated with the venue is stored. A processing device system may be configured to determine a current location of the mobile device, and provide at least some of the venue content data based at least on an analysis of the venue location data, or attraction location data, and the current location.

Abstract: Disclosed herein are, among other things, systems and methods for providing location-based digital stories to a user of processing a device system, such as a mobile device. In some embodiments, a user profile associated with the user and data indicating a plurality of location-specific digital stories related to a common theme at a plurality of locations may be stored. A processing device system may be configured to determine a current location of the mobile device and to provide a first or second digital story to the mobile device based on an analysis of the stored user profile and the current location.

Abstract: Designing a drillstring in for use in a wellbore, such as a deviated or horizontal wellbore, particularly with application to extended reach wellbores. Method comprises determining the downhole weight-on-bit transmitted by a drillstring by determining a baseline tension profile and baseline torque profile in a drill string rotating off-bottom, and then using such profiles in conjunction with a selected surface weight-on-bit slack-off value to calculate a drillstring tension profile and rotating torque profile with the bit on bottom and having weight applied thereto. The method includes buckling limit checks and rig operational limit checks to determine if sufficient torque and DWOB can be transmitted through the drillstring, and to determine whether the drill string remains operable even though portions thereof may be in a buckled condition.

Abstract: Designs for multiple segment lobe pumps are shown. The designs include pumps using rotors having two lobes to a plurality of lobes and segments that include two segments to a plurality of segments. Designs for both vertical, or straight walled conventional lobed rotors, as well as helical lobe rotors are shown. The designs are applicable to a variety of rotors and number of segments. In one particular case the designs enable a three lobe helical pump. Designs are also shown for separation plates used between the multiple segments. The separation float between the pairs of lobes in a segment and can also have a fixed position between the lobes by inclusion of end pieces that enable clamping of the separation plates in position.

Abstract: A transportable and modular field-deployable hydrolysis system for neutralizing toxic chemical agents is described. The system comprises a transportable chemical process plant that is capable of operating in a remote location without significant site infrastructure. The system is also capable of operating in a maritime, shipboard environment. A flexible design allows processing of chemical agents in both batch and continuous flow modes, as well as collection and accumulation of agents from a variety of containers whose form and size may not be known until the system is on-site.

Abstract: Designs for multiple segment lobe pumps are shown. The designs include pumps using rotors having two lobes to a plurality of lobes and segments that include two segments to a plurality of segments. Designs for both vertical or straight walled conventional lobed rotors as well as helical lobe rotors are shown. The designs are applicable to a variety of rotors and number of segments. In one particular case the designs enable a three lobe helical pump.

Abstract: Designs for multiple segment lobe pumps are shown. The designs include pumps using rotors having two lobes to a plurality of lobes and segments that include two segments to a plurality of segments. Designs for both vertical, or straight walled conventional lobed rotors, as well as helical lobe rotors are shown. The designs are applicable to a variety of rotors and number of segments. In one particular case the designs enable a three lobe helical pump. Designs are also shown for separator plates used between the multiple segments. The separator float between the pairs of lobes in a segment and can also have a fixed position between the lobes by inclusion of end pieces that enable clamping of the separator plates in position.

Abstract: A method for actuating a solenoid can include, when the solenoid is in the unactuated position: (i) providing a control signal at a first voltage level to the solenoid, and (ii) adjusting the control signal to a second voltage level to actuate the solenoid to the actuated position. The first voltage level can be greater than zero but less than a voltage sufficient to switch the solenoid to the actuated position. When the solenoid is in the actuated position, the control signal can be adjusted to a third voltage level (less than the second voltage level and less than a voltage insufficient to maintain the solenoid in the actuated position) to switch the solenoid to the unactuated position. The control signal can be adjusted to the first voltage level after the solenoid has returned to the unactuated position.

Abstract: Disclosed herein are, among other things, systems and methods for providing location-based digital stories to a user of processing a device system, such as a mobile device. In some embodiments, a user profile associated with the user and data indicating a plurality of location-specific digital stories related to a common theme at a plurality of locations may be stored. A processing device system may be configured to determine a current location of the mobile device and to provide a first or second digital story to the mobile device based on an analysis of the stored user profile and the current location.

Abstract: Disclosed herein are, among other things, systems and methods for providing digital content for multiple venues to mobile devices. In some embodiments, venue location data defining a geographic boundary of each of a plurality of venues and venue content data for a plurality of different attractions associated with the venue is stored. A processing device system may be configured to determine a current location of the mobile device, and provide at least some of the venue content data based at least on an analysis of the venue location data, or attraction location data, and the current location.

Abstract: Methods and systems for detecting downhole bit dysfunction in a drill bit penetrating a subterranean formation comprising receiving a plurality of drilling parameters characterizing a wellbore drilling operation and calculating bit aggressiveness (?) at each of a plurality of points during drilling, wherein each point corresponds to time, depth, or both. A depth-of-cut (DOC), time derivative of bit aggressiveness ({dot over (?)}), calculated as d?/dt, or both, is calculated at each of the plurality of points. A two-dimensional data representation of the plurality of points, comprising ? in one dimension and DOC, {dot over (?)}, or both, in another dimension is created. Data features are extracted from the two-dimensional data representation and downhole bit dysfunction is identified by comparing the extracted data features with predefined criteria.