Abstract: Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.

Abstract: Crosslinking reagents and methods for using the same for analysis of protein-protein interactions, are provided. The crosslinking reagents include a trifunctional scaffold that links two protein linking groups to each other and branches to link an affinity tag, where the protein linking groups can be fragmented from the scaffold. The distance between the two protein linking groups can be selected to crosslink two proteins of a protein complex via accessible amino acid residues. Also provided are crosslinked polypeptide compounds and kits that include crosslinking reagents. These reagents and methods find use in a variety of applications in which crosslinking of proteins in desired.

Abstract: An improved riser system which comprises a connector for connecting conduits and a mooring system for mooring the connector to the floor of a body of water. The connector may include a pivoting device. The improved riser system also comprises a buoy system for supporting the connector. The buoy system is configured to provide a fixed buoyancy for the connector, the mooring system and at least a portion of the conduits and for providing variable buoyancy for placement of the connector at a predetermined water depth. The improved riser system may also include a flexible conduit made of titanium.

Abstract: A relatively low frequency chopping operation is applied to a delta-sigma ADC to reduce DC offsets resulting from non-ideal component operation. Sequential chopping takes place outside a closed loop and may include an inverted polarity feedback for a part of the chopping period. Nested chopping involves chopping within the closed loop, and may include an inverted polarity feedback and a time shift. The feedback compensation for sequential and nested chopping permits the correct polarity feedback to be provided at the desired time in conjunction with sampling and quantization events. Integrating capacitor(s) may be swapped in relative polarity during nested chopping to preserve residual conversion information for the desired polarity. The ADC operation is non-temperature dependent and avoids modification to the useful signal, resulting in higher accuracy.

Abstract: A relatively low frequency chopping operation is applied to a delta-sigma ADC to reduce DC offsets resulting from non-ideal component operation. Sequential chopping takes place outside a closed loop and may include an inverted polarity feedback for a part of the chopping period. Nested chopping involves chopping within the closed loop, and may include an inverted polarity feedback and a time shift. The feedback compensation for sequential and nested chopping permits the correct polarity feedback to be provided at the desired time in conjunction with sampling and quantization events. Integrating capacitor(s) may be swapped in relative polarity during nested chopping to preserve residual conversion information for the desired polarity. The ADC operation is non-temperature dependent and avoids modification to the useful signal, resulting in higher accuracy.

Abstract: An extendable cleaning tool with multiple, cleaning, agitating and wiping implements, each being simultaneously in ready-for-use configuration and proximity during a waterless cleaning operation, requiring axial rotation of the tool's pole for positioning for use and without requiring multiple repositioning of the tool's distal end between the user's location and the proximity of a to-be-cleaned surface area during waterless cleaning of that surface area.

Abstract: An integrated shipping scheme enables seller users of a third-party payment service to facilitate shipping transactions in connection with payment transactions for items sold by the sellers. Shipping information is automatically gathered and/or entered by the seller via a computer interface hosted by the third-party payment service and sent “behind the scenes” to a selected shipping vendor. The shipping vendor processes the shipping information, and returns shipment data, including data to generate a shipping label. A web page containing the shipping label is then generated and served to a client operated by the seller and displayed on a browser screen, enabling the shipping label to be printed out by the seller. At the same time, electronic payment transfer operations are performed to effectively transfer payment from the seller directly to the shipping vendor via the third-party payment service in a manner that is transparent to both the seller and the shipping vendor.

Abstract: A low cost method of attaching an actuator arm assembly to a single head hard disk drive that has a reduced part count The actuator arm assembly includes a suspension arm stamped from a metal and a pivot bearing cartridge contained by a reduced mass outer sleeve.

Abstract: A system and method for facilitating a financial transaction over a network including use of a passphrase account identifier is described herein. In one embodiment, a system for facilitating a financial transaction over a network comprises a communication interface; and a payment provider system configured to receive via the communication interface a passphrase account identifier from a merchant system, match the passphrase account identifier to a corresponding funding instrument number of a corresponding funding instrument, communicated the corresponding funding instrument number to an issuer system, receive from the issuer system a notification indication of one of acceptance of the funding instrument or decline of the funding instrument, and communicate back to the merchant system the notification indication.

Abstract: A printer driver may be used to pass transaction information to a remote location for storage. Such transaction information may pertain, for example, to online transactions performed by a user in relation to various online merchants. Such transactions, for example, may also be facilitated by a payment service provider. In one example, a method of processing transaction information includes receiving transaction information at a client device. The transaction information is associated with a user-merchant transaction. The method also includes receiving a user-initiated selection of a printer driver. The method further includes, in response to the selection, passing the transaction information to a remote location to store the transaction information in a transaction record maintained at the remote location. The passing is performed by software implemented in the printer driver. Related methods, devices, and systems are also disclosed.

Abstract: A method of treating human and/or animal waste products comprising contacting the waste products with an effective amount of Fusarium culmorum and Muscodor albus, together with a buffering agent and starch. The treatment process covered by the present invention can be employed in connection with pit toilets, portal a toilets, disposable waste bags, or any other environment in which the treatment of human and/or animal wastes is desired. Both the Fusarium culmorum and the Muscodor albus can be stored on infested seed grains, including, but not limited to, barley, rye, rice, wheat, mustard and grass. A method or preparing Fusarium culmorum for use in the treatment of human and/or animal wastes. An isolated culture of Fusarium culmorum. A composition comprising the same four elements used in the treatment process. A method for controlling the pH of a mixture of human liquid and sold wastes.

Abstract: The invention provides, in some aspects, devices for image acquisition that use seals (e.g., O-rings) between concentrically disposed portions of an enclosure and an optics assembly (or sub-assemblies thereof) in order to protect image acquisition components from the surrounding environment (and vice versa) while, at the same time, providing adequate friction for both adjusting and locking focus. Such devices include, in one aspect of the invention, an image capture medium (e.g., a CMOS sensor, CCD array, etc.) that is disposed within an enclosure and an optics assembly that is also disposed within that enclosure, rotatably. The optics assembly, which includes at least a lens, can have a cylindrical outer diameter along at least a portion of its length that is received within the enclosure along a length that has a corresponding cylindrical inner diameter. A first seal (e.g., an “O-ring”) is disposed between, and in contact with, the optics assembly and the enclosure, e.g., along these corresponding lengths.

Abstract: An container-attachable beverage container coaster with a container attachment member dynamically attached to the coaster base for engagement with beverage container bottom surfaces of varying sizes and contours.

Abstract: A panel assembly for covering a storage recess in a vehicle floor includes a base panel and an extension panel. The base panel is adapted to be movable relative to the recess to cover a first portion of the recess. The extension panel is slidably mounted on the base panel and movable with the base panel. The extension panel is slidable between a retracted position in which a portion of the extension panel is adjacent the base panel, and an extended position such that the portion of the extension panel extends outwardly from the base panel and is adapted to cover a second portion of the recess.

Abstract: A center seat console that moves between a forward position between front seats, an intermediate position, and a rear position between rear seats is disclosed. The seat/console may function as a seat in the rear seat position, or in the intermediate position. In the intermediate position, the seat/console in the seat configuration may advantageously be used to support a child safety seat. The seat/console may also be configured as a console in either the rear seat, intermediate, or front seat positions. The seat/console is provided with an adjustable height support that permits the seat/console to be lowered to fit beneath arm rests assembled to the front seats when it is desired to place the console between the front seats.

Abstract: This invention provides a Graphical User Interface (GUI) that operates in connection with a machine vision detector or other machine vision system, which provides a highly intuitive and industrial machine-like appearance and layout. The GUI includes a centralized image frame window surrounded by panes having buttons and specific interface components that the user employs in each step of a machine vision system set up and run procedure. One pane allows the user to view and manipulate a recorded filmstrip of image thumbnails taken in a sequence, and provides the filmstrip with specialized highlighting (colors or patterns) that indicate useful information about the underlying images.

Abstract: An expandable sealant is provided for a fastener including a threaded defining surface. The fastener includes the expandable sealant as a coating adhered to at least a portion of the thread-defining surface to lock the fastener in place and to expand to fill space between the thread-defining surface of the fastener and an aperture through which the fastener is passed to seal the aperture to prevent fluid and gases from passing therethrough.

Abstract: Compounds represented by Formula (I): (I) or pharmaceutically acceptable salts thereof, are effective as NMDA NR2B antagonists useful for relieving pain

Abstract: A vehicle seat assembly is attached to a floor attachment bracket by pivoted front and rear leg assemblies is disclosed. A seat base frame, the floor attachment bracket and the front and rear leg assemblies form a four bar link that support the seat assembly on a floor pan of a vehicle. The floor pan defines a stowage well in which the seat assembly may be selectively stored. The four bar link may be rotated to a limited extent when the seat back frame is in its normal seating position to provide a tip and kneel position. In the tip and kneel position the seat is disposed intermediate the seating position and fully stowed position with the seat back upright to provide improved ingress and egress to areas of the vehicle behind the seat assembly.

Abstract: Compounds represented by Formula (I) or pharmaceutically acceptable salts thereof, are effective as NMDA/NR2B antagonists useful for treating neurological conditions such as, for example, pain, Parkinson's disease, Alzheimer's disease, epilepsy, depression, anxiety, ischemic brain injury including stroke, and other conditions.