Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Compositions comprising modified recombinant polymerizing enzymes are provided, along with nucleic acid molecules encoding the modified polymerizing enzymes. In some aspects, methods of using such polymerizing enzymes to synthesize a nucleic acid molecule or to sequence a nucleic acid template are provided.

Abstract: The present disclosure relates to methods and compositions for protein identification via peptide barcodes.

Abstract: Provided herein are novel amino acid binding proteins that recognize aspartate and/or glutamate in protein sequencing reactions; novel amino acid binding proteins that recognize arginine in protein sequencing reactions; and novel amino acid binding proteins that recognize glycine, alanine, and/or serine in protein sequencing reactions.

Abstract: Aspects of the technology described herein relate to improved semiconductor-based image sensor designs. In some embodiments, an integrated circuit may comprise a photodetection region and a drain region electrically coupled to the photodetection region, and the photodetection region may be configured to induce an intrinsic electric field in a direction from the photodetection region to the drain region(s). In some embodiments, an integrated circuit may comprise a plurality of pixels and a control circuit configured to control a transfer of charge carriers in a plurality of time-binning pixels. In some embodiments, an optical component for optical rejection is provided in between a waveguide and the time-binning pixel and configured to block at least some excitation photons in a pulsed light stream from arriving at the photodetection region.

Abstract: There is provided amino acid cleaving reagents with improved cleavage activity, allowing for more structural information to be obtained from polypeptides in sequencing reactions.

Abstract: Systems and method are provided for detecting the display of media and/or specialized content using embedded watermarks. For example, a media device may receive video data including a set of video frames. The media device may detect, in at least one video frame of the set of video frames, a watermark embedded into the at least one video frame. The watermark may be usable to identify media to be displayed by the media device. The media device may display the media. The media device may then transmit an indication that the media has been presented by the media device to a destination device.

Abstract: The disclosure provides methods and compositions that enable the characterization of analytes in a sample, including the identification of polypeptides having one or more post-translational modifications. In some embodiments, the disclosure provides methods of determining a concentration of an analyte in a sample based at least in part on a count of detected series of signal pulses. In some embodiments, the disclosure provides methods of determining one or more chemical characteristics of an analyte (e.g., a polypeptide). In some embodiments, the disclosure provides a method (e.g., a single-molecule method) comprising contacting a single polypeptide with one or more post-translational modification-specific (PTM-specific) affinity reagents; and identifying whether the single polypeptide comprises a post-translational modification (PTM) by determining a luminescence signature representative of the binding interaction(s) between the single polypeptide and the one or more PTM-specific affinity reagents.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Systems and methods are provided for decoding watermarks in video frames. A media device may receive a video frame that includes a first predetermined region comprising a watermark and a second predetermined region having pixel values selected to reduce the perceptibility of the first predetermined region. The media device may detect the watermark in the first predetermined region of the video frame and identify one or more contiguous subsets of pixels that correspond to a first pixel value and one or more contiguous subsets of pixels that correspond to a second pixel value. The media device then assigns a first symbol to the one or more contiguous subsets of pixels that correspond to the first pixel value and second symbol the one or more contiguous subsets of pixels that correspond to the second pixel value. The media device then generates a first sequence of symbols from the assigned symbols.

Abstract: Methods, systems, devices, and computer-program products are described herein for providing dynamic content serving. The dynamic content serving technology can identify, in real-time, programming arriving at a client device, identify a specific media segment being received and/or displayed, and determine which pre-stored substitute media segment may be used to replace the identified segment. A picture-in-picture channel can be used to display the substitute media segment.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: The disclosure provides methods and compositions that enable the identification of polypeptides having one or more post-translational modifications. In some embodiments, the disclosure provides a method (e.g., a single-molecule measurement method) comprising contacting a single polypeptide with one or more post-translational modification-specific (PTM-specific) affinity reagents; and identifying whether the single polypeptide comprises a post-translational modification (PTM) by determining a luminescence signature representative of the binding interaction(s) between the single polypeptide and the one or more PTM-specific affinity reagents.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: A baking pan oiling device includes a housing for receiving a plurality of baking pans therethrough during a pan oiling operation, an oil supply fluidly coupled to a plurality of nozzles, and a pump for causing the nozzles to spray oil into at least one orifice of each of the baking pans. A sensor arranged downstream of the nozzles generates signals corresponding to a sensed presence or absence of oil in the baking pan, and a control unit determines the pan oiling device requires attention based on such signals, including causing the pan oiling operation to be stopped, transmitting a message, and/or causing an indicator to be actuated. The sensor may additionally sense pan type and/or color, oil spray pattern on the pan, spray amount on the pan, and so on, and in response, the control unit may generate control signals to facilitate operations of the pan oiling device.

Abstract: Systems and methods for evaluating utility infrastructure using LIDAR data by dynamically capturing and processing the LIDAR data for improved assessment accuracy and processing efficiency. The method includes determining an orientation of a mobile LIDAR system, dynamically capturing LIDAR data corresponding to a region of interest by collecting LIDAR samples based on the orientation and the region of interest, identifying a utility object based at least in part on the LIDAR samples, decimating the LIDAR samples within a predetermined area around the utility object, generating depth-encoded multi-perspective images of the utility object using the decimated LIDAR samples, and determining at least one condition of the utility object. Certain implementations include determining a speed and an orientation of a mobile LIDAR system and dynamically capturing position data corresponding to a region of interest by collecting LIDAR samples at a rate based on the speed, the orientation, and the region of interest.