Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.

Abstract: The present disclosure relates to methods of transforming various species of algae, for example, algae from the genus Scenedesmus and the genus Dunaliella, vectors and nucleic acid constructs useful in conducting such transformations, and recombinant algae, for example, Scenedesmus and Dunaliella produced using the vectors and methods disclosed herein.

Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The present disclosure details a thermoacoustic driven compressor having a pressurized housing, which contains within a thermoacoustic engine and a working gas, coupled to a positive displacement reciprocating compressor. The thermoacoustic driven compressor generates scalable compressed air from a given heat source.

Abstract: The present disclosure details a thermoacoustic driven compressor having a pressurized housing, which contains within a thermoacoustic engine and a working gas, coupled to a positive displacement reciprocating compressor. The thermoacoustic driven compressor generates scalable compressed air from a given heat source.

Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The present disclosure details a thermoacoustic driven compressor having a pressurized housing, which contains within a thermoacoustic engine and a working gas, coupled to a positive displacement reciprocating compressor. The thermoacoustic driven compressor generates scalable compressed air from a given heat source.

Abstract: Apparatus and method for mechanically attached connections of conduits may include a conduit gripping member (34), a drive member (36), and a seal member (48), the drive member causing axial movement of the conduit gripping member to indent into an outer surface of the conduit when the assembly is pulled-up, the drive member causing the seal member to form a zero clearance seal at a location that is axially spaced from the conduit gripping member. The zero clearance seal may comprise a face seal arrangement including a gasket (48), and the conduit gripping member may be a ferrule, ring or other device that can grip and optionally seal against the conduit outer surface. The assembly may include a sensing function for detecting or sensing a characteristic or condition of an assembly component or the fluid or both, hi one embodiment, a body coupling member has a two piece construction of a main body and a conduit socket insert. A flared fitting embodiment is also provided.

Abstract: Vectors capable of stable replication in yeast and bacteria and comprising all essential genes of vascular plant plastids are provided as well as the use of such vectors to construct an recombinant plastid genome and host cells transformed with said vectors and recombinant plastid genomes.

Abstract: A repository manager facilitates robust revision control of components of a large scale system. The repository manager stores system components under revision control in a repository, according to internal names. The repository manager also stores binding information between internal names of system components and associated external names, as well as component attribute information. The repository manager provides users with working areas, from which they are able to access system components according to their external names, via the repository manager. As users perform operations affecting system components, the repository manager keeps track of the performed operations. Responsive to a user committing a set of changes, the repository manager updates the master repository according to the performed operations, associates a new version number with the resulting state of the repository and synchronizes the user's working area with the updated repository.

Abstract: The present invention provides compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.

Abstract: The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.

Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

Abstract: The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.

Abstract: A repository manager facilitates robust revision control of components of a large scale system. The repository manager stores system components under revision control in a repository, according to internal names. The repository manager also stores binding information between internal names of system components and associated external names, as well as component attribute information. The repository manager provides users with working areas, from which they are able to access system components according to their external names, via the repository manager. As users perform operations affecting system components, the repository manager keeps track of the performed operations. Responsive to a user committing a set of changes, the repository manager updates the master repository according to the performed operations, associates a new version number with the resulting state of the repository and synchronizes the user's working area with the updated repository.

Abstract: A repository manager facilitates robust revision control of components of a large scale system. The repository manager stores system components under revision control in a repository, according to internal names. The repository manager also stores binding information between internal names of system components and associated external names, as well as component attribute information. The repository manager provides users with working areas, from which they are able to access system components according to their external names, via the repository manager. As users perform operations affecting system components, the repository manager keeps track of the performed operations. Responsive to a user committing a set of changes, the repository manager updates the master repository according to the performed operations, associates a new version number with the resulting state of the repository and synchronizes the user's working area with the updated repository.