Abstract: A water resistant article for positioning on an appendage to be treated comprises a knitted body constructed from synthetic yarns is provided, wherein each of the synthetic yarns comprise a bundle of substantially parallel fine monofilaments.

Abstract: An orthopedic padding including an elongate spacer fabric having spaced faces interconnected and separated by an intermediate spacer area, the spaced faces having a knitted construction different from a knitted construction of the intermediate spacer area, and the elongate fabric constructed at least in part of hydrophobic monofilament yarn knitted in an open construction for breathability.

Abstract: A medical bandaging product including a sealable enclosure and a resin-impregnated substrate including a knitted fabric layer having upper and lower surfaces with interconnected knitted fabric yarns knitted such that at least one yarn extends across a gap from the upper surface to the lower surface along a width of the substrate in a back-and-forth pattern forming a single, integrated three-dimensional structure, wherein the at least one yarn extends between non-adjacent needle positions in a back-and-forth pattern in successive courses to provide a continuous pattern on both the upper and lower major surfaces of the substrate, wherein needle bed gap spacing is between 7.1 mm and 8 mm and a total thickness of the substrate is between 4.75 mm and 4.90 mm.

Abstract: Compositions and methods are provided for enhancing enzymatic ligation between nucleic acid fragments that relies on one or more small molecule enhancers having a size of less than 1000 daltons. For example, enhancement of ligation efficiencies are observed for double-stranded nucleic acid fragments that are blunt-ended, have a single nucleotide overhang at the ligation end, or have staggered ends compared to ligation under similar conditions in the absence of the one or more small molecule ligation enhancer. The use of small molecule enhancers for ligating nucleic acids results in an increased number of transformed host cells after transformation with the ligated molecules. This enhancement can be observed with chemically transformed host cells and with host cells transformed by electroporation.

Abstract: Compositions and methods are provided for ligating polynucleotides having a length that is greater than 8 nucleotides on an RNA splint. The ligation reaction provides consistent results in high or low ATP concentrations. The reaction can occur rapidly and is generally at least 10 fold more efficient than T4DNA ligase under optimal conditions for T4DNA ligase and the reaction time is less than 6 hours for example, less than 1 hour.

Abstract: Wireless audio headset systems and methods for automatically activating target application programs on an attached processor-based host are disclosed. The system generally includes a headset base to communicate with the host executing a headset integrator application, a wireless headset in wireless communication with the headset base and configured to transmit audio signals to and from the headset integrator application via the headset base, and a program activation mechanism provided by the wireless headset that, upon activation of the mechanism, activates a target application program on the processor-based host via the headset base and the headset integrator application.

Abstract: Compositions and methods are provided that relate to solubilized phospholipids and their use in stabilizing nucleic acid polymerases. For example, a phospholipid with a tail containing at least 8 carbons can be solubilized in the presence of an amphipathic molecule.

Abstract: Compositions and methods are provided that relate to solubilized phospholipids and their use in stabilizing nucleic acid polymerases. For example, a phospholipid with a tail containing at least 8 carbons can be solubilized in the presence of an amphipathic molecule.

Abstract: A low density fabric substrate for a medical bandaging product. The substrate has a plurality of longitudinally-extending voids formed in the fabric for permitting water penetration through the fabric thickness from one of the major faces to the other major faces. A reactive system is impregnated into or coated onto the substrate and remains stable when maintained in substantially moisture-free conditions and hardens upon exposure to sufficient moisture to form a rigid, self supporting structure. A soft, flexible protective material covers at least one of the major faces of the substrate along its length to provide a cushioning barrier between the substrate and the skin of a patient when the material is in use.

Abstract: Compositions and methods are provided that relate to solubilized phospholipids and their use in stabilizing nucleic acid polymerases. For example, a phospholipid with a tail containing at least 8 carbons can be solubilized in the presence of an amphipathic molecule.

Abstract: Compositions and methods are provided for enhancing enzymatic ligation between nucleic acid fragments that relies on one or more small molecule enhancers having a size of less than 1000 daltons. For example, enhancement of ligation efficiencies are observed for double-stranded nucleic acid fragments that are blunt-ended, have a single nucleotide overhang at the ligation end, or have staggered ends compared to ligation under similar conditions in the absence of the one or more small molecule ligation enhancer. The use of small molecule enhancers for ligating nucleic acids results in an increased number of transformed host cells after transformation with the ligated molecules. This enhancement can be observed with chemically transformed host cells and with host cells transformed by electroporation.

Abstract: The present invention is a two or more subject retractable leash retractable leash, and methods of use regarding the same, which includes a roll-back feature on the retractable leash. The leashes may be locked in place by engaging a single lever. The leashes retract regardless of whether the spools are locked or unlocked. The leashes retract independently of the other leashes, such that a dual leash may be used to walk one subject.

Abstract: A spectrum use assessment server receives spectrum use data from each of plural electronic devices. The spectrum use data from each electronic device includes at least location of the electronic device and operating channel of the electronic device. The server determines a spectrum use coverage area of each electronic device and identifies an overlap in the coverage areas in which two or more of the electronic devices engage in competing spectrum use.

Abstract: The present invention is a two or more subject retractable leash retractable leash, and methods of use regarding the same, which includes a roll-back feature on the retractable leash. The leashes may be locked in place by engaging a single lever. The leashes retract regardless of whether the spools are locked or unlocked. The leashes retract independently of the other leashes, such that a dual leash may be used to walk one subject.

Abstract: The present invention is a two or more subject retractable leash retractable leash, and methods of use regarding the same, which includes a roll-back feature on the retractable leash. The leashes may be locked in place by engaging a single lever. The leashes retract regardless of whether the spools are locked or unlocked. The leashes retract independently of the other leashes, such that a dual leash may be used to walk one subject.

Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.

Abstract: Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.

Abstract: A steam generator system for a pressurized water reactor which employs an external to containment steam drum and recirculation loop piping. The steam generator system changes the arrangement of a typical pressurized water reactor recirculation steam generator by relocating the functions of steam separation and feedwater preheating outside of the reactor coolant system. The steam generator system and thermal hydraulic conditions are selected in order to minimize the size of the steam generator heat exchanger component volume inside of the containment. The external steam drum component can be isolated in accident conditions when desired and is used as a source of secondary fluid inventory for improved decay heat removal capability and tolerance for loss of feedwater events. Thus, the steam generator component volume inside of the containment is reduced and the amount of maintenance required for the reactor coolant system components are similarly reduced.

Abstract: Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.

Abstract: Compositions are provided that include a plurality of small molecules selected from the group consisting of an amide, urea or acetone having a molecular weight less than 300 g/mol; and dNTPs and a polymerase in a buffer suitable for use as an amplification buffer. Methods of use of the compositions are also described for reducing non-template DNA amplification.